Biotechnology: Introduction to Gel Electrophoresis Go to:

http://www.classzone.com/cz/books/bio_07/resources/htmls/virtual_labs/virtualLabs.html

Name: ________________________________________________ Explore: Define the following/Describe what is it used for: * Agarose Gel: se! to view "NA # "NA $arkers: "NA %ragments with known lengths # "igeste! "NA &amples: # 'lectrophoresis (hamber: # )ower &uppl*: # +romophenol +lue: se! to see how %ar the "NA samples have move! # ,+' +u%%er: se! to help carr* electric currents # Gel &taining ,ra*: )lace where electrophoresis takes place # 'thi!ium +romi!e: -luorescent !*e use! %or staining "NA # $icropipette: se! to trans%er "NA an! !*e # )ipette ,ips: se! with micropipettes. new one must be use! with each use # "eionize! /ater: se! to rinse the gel a%ter it is staine! # 0 Light +o1: se! to help rea! the gel a%ter it is straine! Procedure: Step #1: Explain how comparing DNA Fingerprints can help identify a person who has committed a crime Go through the steps #2-11 Record the BASICS o% each step below: (over the Gel with the bu%%er 2nsert the each "NA %oun! %rom the scene into the gel with ++ ,urn on the power suppl* with positive %acing the other !irection o% the wells /ait %or 3 hour )lace gel in !ish an! %ill it with bromi!e an! let it sit %or 40 minutes an! then empt* it

-ill the !ish with the gel with !eionize! water an! let it sit %or 40 minutes then empt* )lace the gel on the 0 tra*. it shoul! be rea!able now. 5ecor! the in%ormation

Step #12: Questions 3: Ev lu te: Does the DNA found on the hair sample match suspect #1 or suspect #2 !ow do you "now
Suspect 2 because the bands in the DNA match

6: An ly!e: #hy do a series of $ands appear on the gel ,he* show the "NA se7uence which can be use! to i!enti%* the criminal. 4: Identi"y C use: #hy is the largest DNA fragment $and found closest to the well in which it was placed
Positive charges attract the DNA so the largest bands will be closer the positive charge .

8: In"er: #hat is true of the DNA fragment $and closest to the positi%e end of the gel
That it is the smallest due to the fact that it was not stopped earlier on.

9: Predict: #hat would happen if the electrodes were plugged into the wrong outlets
The test would not work because the DNA wouldnt be able to go in the other direction of the gel.

:: Apply: #hy is DNA fingerprinting more conclusi%e when pro%ing a person&s innocence rather than their guilt Everyone has a unique strand of DNA so if the DNA found at the scene does not match the DNA of the innocent suspect then the suspect is safe to go home but if one of the suspects DNA is identical to the one found then they are the culprit and there is no way they can disprove it.