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Microbes are grouped under Protista, which is further divided into the
Eukaryotes and the Prokaryotes. Eukaryotes comprise the Algae, Protozoa,
Fungi and the Slime Molds. Prokaryotes comprise the Bacteria and the Blue
green Algae.
PROKARYOTES
These are single-celled organisms, which have a 70S ribosomes, whereas the
eukaryotes have the 80 S ribosomes. These are important since certain
antibiotics target the 70 S ribosomes and affect protein synthesis.
Histones are proteins which have no enzymatic activity, but are involved in
DNA packaging. There are five types: H1, H2A, H2B, H3 and H4.
They have structures such as the flagella, pili, fimbriae and a cell wall.
BACTERIAL NOMENCLATURE
The names always have the genus and the species name (e.g.
Staphylococcus aureus). These namescome about due to the bacteria’s
morphology, biochemical properties and metabolic differences. The
classification has become more detailed now since the bacteria’s
immunologic and genetic characteristics are included.
The cell envelope is defined as all the layers that enclose the cytosol of a
bacterium. The cell wall is the peptidoglycan layer ONLY.
(100-800 A)
None Endotoxin/Lipid A
None Porins
None Murein
The cytoplasmic membrane is similar for the Gram (+) and Gram (-) bacteria.
It is a lipid bilayer, which is made up of lipids and proteins. All the lipid
components are amphipathic: the polar had interacts with the aqueous
environment, while thetails interacts with one another to form the interior of
the bilayer. Unlike animal cytoplasmic membrane, bacteria do NOT have
cholesterol in their cytoplasmic membrane. It does have 70 % protein, 20-
30% lipid and small amounts of carbohydrates. It also has embedded
proteins, which aid in its function. Mesosomes, or invaginations in the
cytoplasmic membrane, maybe the site of DNA replication.
1. Osmotic barrier
CELL WALL
1. D-glutamic acid
2. D-alanine
3. Diaminopimelic acid
1. Shape
2. Barrier
3. Sieve
4. Prevents lysis
5. Aids In dessification
TECHOIC ACID
This is an important polysaccharide in the Gram (+) cell wall since it acts as
an antigenic determinant. It is also called the virulence factor.
1. Proteins
2. Peptidoglycans
3. Hydrolytic enzymes
4. Penicillinases
Lastly, the Gram (-) bacteria have an outer membrane. In this outer
membrane, there are the following:
1. Lipopolysaccharides
2. Somatic O antigen
3. Core polysaccharides
4. Porin proteins
Core polysaccharide
Lipid A
The O-specific side chains are oligosaccharides and will always differ from
one organism to another, so they can be used for antigenic determinants.
b. Prevents phagocytosis
7. Pili, similar to the flagella, but shorter; function for adherence, and for
reproduction. This is mostly present in Gram (-) bacteria.
Review Questions:
What is the structure that totally encloses the cytosol of the bacterium?
CELL ENVELOPE
SELF-STUDY
Kr/08