CHE 451 Biochemistry homework #2

Due 4pm Thursday Jan 23 2!14" #5 points $isted % 5! points possi&$e There are 75 points listed here: about 25 pts worth will be selected for grading by your instructor; All the remaining questions will be scored for completion only, to add up to 50pts total A complete answer !ey to A"" questions will be made a#ailable on $lac!board %lease do not share it &nor any homewor! !ey' with future biochem students (se of any pre#ious year)s homewor! !ey to answer questions in this homewor! is academic dishonesty 1 (6 pts) The human blood clotting factor Xa has an amino acid composition as follows: Ala (A) 24 (5 !") #l$ (#) %6 (& !") 'ro (') 1& (4 4") Arg (() 22 (5 4") )is ()) * (1 *") +er (+) 2% (5 *") Asn (,) 16 (4 -") .le (.) 16 (4 -") Thr (T) %% (& 1") Asp (/) 25 (6 2") 0eu (0) 26 (6 4") Trp (1) 5 (1 2") 2$s (2) 22 (5 4") 0$s (3) 2& (6 !") T$r (4) 12 (% -") #ln (5) 1& (4 4") 6et (6) 5 (1 2") 7al (7) 2(4 !") #lu (8) %1 (* *") 'he (9) 1& (4 4") 1hat would be the net charge on this protein in blood: where the p) is * 4; <<<<< .f $ou had this protein in a test tube at p) 5: what would its net charge be; <<<<< This protein is acti=e outside of cells )ow might the 2$s residues affect its structure; 2 (6 pts) 0oo> up 6A'3 on #oogle or elsewhere 1hat >ind of post?translational modification of amino acids do these en@$mes cause; 1hat >ind of process are the$ in=ol=ed with; 1h$ are some 6A'3As classified as oncogenes; % (& pts) .n $our own words: describe the secondar$: tertiar$ and Buaternar$ structure of >inesin (see teCtboo> p 144?146D14&?15-) .nclude microtubules in $our answer 4 (6 pts) .n two or three sentences: compareDcontrast fibrous and globular proteins 5 (% pts) 1hat common purpose do ?helices and ?sheets ser=e; Eown words: one sentenceF 6 (! pts) /escribe the h$drogen bonding pattern of an ?heliC E1hat does i: iG4 mean?F /escribe the h$drogen bonding pattern of an anti?parallel ?sheet /escribe the h$drogen bonding pattern of a parallel ?sheet * (5 pts) 1h$ will the phiDpsi angles of an amino acid in an ?heliC be different from the phiDpsi angles of an amino acid in a ?sheet; )ow are the phiDpsi angles possible for 'ro and for #l$ different from those of the other amino acids; & (6 pts) .n what T1H wa$s is entrop$ in=ol=ed in protein folding; ! (6 pts) 8Cplain the statement: secondar$ structure does not determine protein folding: but it is reBuired in order for folding to occur 1(4 pts) 1e ha=e been tal>ing about proteins that are soluble in water: where the h$drophobic effect causes proteins to fold so that more of their non?polar residues will be protected from water 6an$ proteins are inserted into (and right through) nonpolar cell membranes )ow might this difference in en=ironment affect the wa$ that membrane proteins are organi@ed: =s water? soluble ones; 11 (6 pts) 1h$ do proteins tend to aggregate after the$ unfold; 1h$ does increasing the temperature tend to cause proteins to unfold; 1hat other factor can cause proteins to unfold; 12 (& pts) )ow does #ro80D8+ wor>; Additional stud$ Buestions (could be on Bui@I) 2hapter 4 problems: 1&: 21: 22: 25: 2*?2!: %1: %2: %4: %6: %* (2nd edition) 2!: %%: %4: %*: %!?41: 4%: 44: 46: 4&: 4! (%rd edition)