LD-50 LAB

Initial Questions: 1. Would 10% concentration have more death than the other? 2. Will there be any death in 0%? Time (Minutes) Treatment 0% Initial 5 Observation Wiggling 6-7 Wiggling 4 not moving* 8 Wiggling 2 not moving 2 moving 8 not moving Clumping and wiggling 1 not moving Clumping and wiggling 10 2dead 8 not moving 3 Dead 7Not moving 4Dead 3Not moving 3 moving Stressed 0 dead 15 2 Dead 8 Not moving 1Dead 9Not moving 2Dead 8Not moving Stressed All moving 20 2Dead 8 Not moving 1Dead 9Not moving 3Dead 7Not moving Stress 0 dead 30 2 dead Final 2/10= 20% dead 1/10= 10% dead 6/10= 60% dead 0/10= 0% dead

0.01%

Wiggling

1 dead

0.1%

Wiggling

6 Dead

1%

Wiggling

Stressed 0dead

10%

Wiggling

All moving stressed

1 not moving

2dead 1 not moving

dead

3/10 = 30% dead

*Not moving- still alive but it doesnt move

70 Death of Blackworms (Percent) 60 50 40 30 Death of Blackworks(Percent) 20 10 0 0 0.01 0.1 1 10 Concentration of CuSO4 (Percent)

Follow-up questions: 1. Review your initial questions before starting the lab. Did you answer these after finishing the lab? Explain. Both of the initial questions were solved. Both were falsified. 0.1% concentration had more deaths than 10%, but in 10% they were always moving because they were stress. Surprisingly there was 2 deaths for 0% concentration, so it was false. 2. Look at the graph you made above of the % of blackworms that died at each concentration of copper sulfate. What is the LD-50 that you would calculate from this data? Somewhere between 0.01 to 0.1 concentration but more to 0.1%.

3. How would your answer change if you were trying to determine the ED-50 (effective dose, or dose where 50% of the blackworms changed behavior)? Hint: Think about what concentration of copper made about half of the blackworms stop moving unless poked, etcIs this value less than the LD-50? Why? It would be 0 to 0.01% concentration because half of them stop moving. It is less because the effective dose just has to affect them. In LD-50 50% of the population dies. You need less for ED-50

4. If you were the policy-maker in charge of determining the highest level of copper that should be allowed in San Diego Bay, what concentration would you choose? Why? Less than 0.01% concentration of CuSO4 because around that range is were ED-50 would be. We dont want the living organism to die. And there would be a lot of boats so the CuSO4 would go up, so less than 0.01% would just do the job. Ill protect most of the living organisms.