BIOHELLENIKA SERVICES

1.
PLATELET RICH PLASMA (PRP)
Platelet Rich Plasma (PRP) is an autologous advanced skin rejuvenation product derived from patient's own blood as a serum enriched with growth factors from platelets and bioactive proteins that induce cell growth and regeneration. This personalized serum aims to stimulate collagen and elastin production of the skin fibroblasts, both molecules fill the desired areas. After a specific process, patient's blood results to a PRP serum, a natural anti-aging solution very beneficial to skin rejuvenation. Also, PRP eliminates acne scars and minimize skin pores leaving your skin smoother and younger. Platelet Rich Plasma is an increasingly popular method as it is the ideal collagen and elastin stimulating therapy for treating ageing skin of face, neck, eyes, hands and dcolletage by improving texture, tone, fine lines and wrinkles, helping it to become progressively thicker, tighter and suppler. Benefits of PRP Therapy PRP method has many advantages such as:
Natural result without the risk of 'looking done' or Minimal discomfort: reduces the pain duration much

faster
Shorten downtime: faster stimulation of healing

process
Cost Effective: the cost is lower compared to

alternative treatments
Simple procedure Long lasting result: rejuvenation lasts for a longer

period of time comparing with other fillers

Not causes trauma

AESTHETICS

VETERINARY

PLASTIC SURGERY

PRP
OPHTHALMOLOGY ORTHOPEDICS

asymmetry
No risk of transmissible infection, rejection, allergic

DENTISTRY

reaction or allergy (100% your own blood by-product)

PLATELET RICH PLASMA (PRP)

PRP APPLICATIONS CLINICAL AREAS Today Platelet Rich Plasma is used to:
Hair loss Fine lines and wrinkles around and under the eyes Wound and scar healing Cheeks and entire face Hand and arms Jaw Neck Chest and dcolletage

AESTHETICS
Manage your skin age a radiant and fresh look just for you
rejuvenate facial skin (including nasolabial lines,

marionette lines, forehead lines), neck chest dcolletage aging

improve the texture of the skin, fine lines, wrinkles,

especially around and under the eyes and neck

improve the quality of aging skin
Loose skin & skin tone

correct acne or other scars post skin laser treatment improve stretch mark back of hands and arms reduce hair loss, strengthen
Hand and arms Other body areas

hair roots and nourish hair follicles

PLASTIC SURGERY
Platelet Rich Plasma stimulates angiogenesis, promoting vascular growth and fibroblast proliferation. In addition, PRP functions as haemostatic by forming a fibrin clot. Also application of PRP enhances wound-healing in both soft and hard tissue.

Biohellenika has developed autologous therapeutic products for the healing of:
pressure sores diabetic ulcers burns wounds

PLATELET RICH PLASMA (PRP)

ORTHOPEDICS
The earlier treatment, the better outcome Except the natural process of ageing, our life itself includes injuries, traumas and pain. PRP is very effective in treating osteoarthritis, especially in early stages. PRP stimulates cartilage regeneration inhibiting the deterioration effects of age and slows the development of arthritis. Biohellenika owns innovative technology and products to enhance soft and bone tissue healing. Most common applications
cartilage/ligament repair osteoarthritis tendonitis post-operative pain inflammation reduction chronic pain

Natural Therapy, No Side Effects

Platelet Rich Plasma (PRP) is a 100% non surgical natural treatment for pain and injury. PRP is injected directly to the site of injury trigging body's nature to repair itself in a drug-free way within a short recovery time for most patients.

Avoid Major Surgery! Heal More Rapidly

DENTISTRY
Platelet rich plasma enhances bone regeneration. Biohellenika is one of the pioneers that developed a new approach to apply PRP
bone graft for closure clefts, lips, fistulas and palate

VETERINARY
PRP applications were first developed in veterinary medicine and now is used for
tendon and ligament injuries wound healing osteoarthritis

defects
repair bone defects after tooth or cysts extraction implant in the dental surgery

1. Treatment With Platelet-Rich Plasma Is More Effective Than Placebo for Knee Osteoarthritis: A Prospective, Double-Blind, Randomized Trial. Patel S, Dhillon MS, Aggarwal S, Marwaha N, Jain A.Am J Sports Med. 2013. 2. A Novel Method For Iatrogenic Vesicovaginal Fistula Treatment: Autologous Platelet-Rich-Plasma Injection And Platelet-Rich-FibrinGlue Interposition. Shirvan MK, Alamdari DH, Ghorifi A. J Urol. 2012 Dec 28

OPTHALMOLOGY
PRP's growth factors quicken the recovery of corneal epithelial cells, shorten the recovery of surgical area by carrying out anti-inflammatory activity, minimize postsurgery pain and stabilize the corrected eyesight by stimulated healing.
epithelial defects trauma of the cornea dry eye syndrome post laser surgery syndrome

2.
AUTOLOGOUS CONDITIONED SERUM (ACS)
Today's applications show that its most common uses are in osteoarthritis, especially in the knee and the hip joint area. As people mature, the percentage of the population that suffers from osteoarthritis increases. A special messenger, the protein interleukin-1 (IL-1) plays an essential role in the development of osteoarthritis. It promotes joint and cartilage inflammation that leads to pain. This protective protein called interleukin-1 receptor antagonist (IL-1Ra) suppresses the aggressive IL-1 and thus acts anti-inflammatory, analgesic and cartilageprotective. Except the natural aging process, other factors such as overweight, genetic predisposition, injuries lead to pain, severity of osteoarthritis and loss of mobility. Beneficiary Effect on Osteoarthritis A unique natural medical procedure in which an autologous blood product is introduced to the patient's body to reduce chronic pain, osteoarthritis and treat arthritis of the elbow, antebrachiocarpal joints, tarsi, phalangeal joints, knee, shoulder, muscular injury in athletes and the sciatica and lumbar back pain. Although osteoarthritis can't be cured yet, symptoms can be treated effectively and degenerative process will be delayed. The Autologous Condition Serum enhances body's natural defense mechanisms against inflammation, reduces pain, improves function and promotes natural healing. A safe, simple and effective treatment.

Concerns All of Us for a New Lifetime

3.
KEEP YOR JUVENILENESS FOR EVER
Discover YOUTH a revolutionary personalized cell product using your own fibroblasts for face rejuvenation Fibroblasts, the richest source of collagen and elastin, are widely spread cells in the body, especially in dermis. Aging decreases all biological activities of fibroblasts like cell proliferation and secretion of extracellular matrix components, thus the skin ages. YOUTH, composed by pure autologous fibroblasts, improves the appearance of your skin. Its implantation enriches the skin with activated cells that produce collagen and elastin for long term rejuvenation of the skin. Fibroblasts are extracted from a small skin biopsy, cultured in Biohellenika's laboratories to reach an adequate number, activated by a low energy laser and injected into the wrinkles and the imperfections of the face. Effective and Simple YOUTH is prepared especially for you!!! The method is safe without any risk, no complications but with long term results. The fibroblasts can be cryopreserved in separated vials and used in more than once future applications. The younger age the fibroblasts, the better results. So, we advise that fibroblasts should be preserved in young ages. Fibroblasts injection usually is combined with PRP for even better results.

4.
MY CREAM
My Cream is a sur mesure revolutionary anti-aging product based on the latest discoveries in stem cell research. This advanced formula completely restores skin's youth, diminishes wrinkles, reverses skin damage and minimizes visible signs of aging. My cream contains high-tech ingredients using the selfrenew abilities of stem cells promoting firmness and elasticity leaving skin smoother and younger-looking. My Cream, since autologous, is personalized for each individual according to the skin type and age. Benefits
Firmer and smoother skin Reduction of fine lines and wrinkles Plumper skin Deeply hydrated skin

Application Apply My cream twice a day (morning & evening) after cleansing. Gently massage in until the cream is absorbed. Size: 30ml

Two Products To Try

Two types of My Cream are available containing a unique anti-aging cream base enriched with two different skincare extracts supporting skin's natural repair process. For the first type, the base is mixed with autologous PRP and for the second we mix the supernatant of the autologous mesenchymal stem cell cultures and the cream base. Both types help cell renewal, have incredible nourishing abilities and prevent the skin from aging prematurely.

5.
MESENCHYMAL STEM CELLS FOR COSMETICS OR REGENERATIVE MEDICINE
Mesenchymal stem cells are collected from adipose tissue after liposuction and are used for cosmetic and regenerative medicine applications. Adipose tissue contains a fraction of stem cells, 1.000 times more than other source. The fraction contains perivascular mesenchymal stem cells from the vessels of microcirculation, vascular endothelial and proendothelial stem cells and mature fibroblasts. In cosmetic medicine, mesenchymal cells are used for face reconstruction combined with PRP, to treat wrinkles and skin rejuvenation. Also, mesenchymal cells are used to treat autoimmune diseases, like multiple sclerosis, Crohn's disease, ulcerative colitis, rheumatoid arthritis and newly diagnosed diabetes type I. Also are used to treat chronic pulmonary obstruction, osteoarthritis (traumatic or degenerated), fractures, diabetic ulcers and burns.
1. Mosna F, Senseb L, Krampera M. Human bone marrow and adipose tissue mesenchymal stem cells: a user's guide. Stem Cells Dev. 2010 Oct;19(10):1449-70. 2. Rada T, Reis RL, Gomes ME. Adipose tissue-derived stem cells and their application in bone and cartilage tissue engineering. TissueEng Part B Rev. 2009 Jun;15(2):113-25. 3. Zhen G, Liu H, Gu N, Zhang H, Xu Y, Zhang Z. Mesenchymal stem cells transplantation protects against rat pulmonary emphysema. Front Biosci. 2008 May 1;13:3415-22. 4. Griffin M, Iqbal SA, Bayat A. Exploring the application of mesenchymal stem cells in bone repair and regeneration. J Bone Joint Surg Br. 2011 Apr;93(4):427-34.

Clinical applications: 1. Cartilage rejuvenation (simple incidents). Mesenchymal stem cells modification to chondrocytes. Biohellenika has developed an innovative technology to isolate, purify and regenerate the patient's own chondroblasts and chondrocytes for either intrarticular or arthroscopic re-injection. In the case of osteoarthritis at any age, mesenchymal cells extracted from adipose tissue modified to chondrocytes and with minimal invasive endoscopy applied into the damaged cartilage. The number of injected cells is analogous to the extent of the damage.

2. Cartilage rejuvenation (for high cartilage lossheavy incidents). Mesenchymal stem cells modification to chondrocytes. Adipose tissue derived stem cells cultured and differentiated into chondrocytes,implanted in three dimensionalscaffold and infused arthroscopically, for cartilage loss over 34 cm2 .

3. Autologous chondrocytes implantation. This method is applied to young patients in the cases of sports injury and not to elder patients, because the ability of chondrocytes to proliferate is decreased during the age. A small biopsy of the healthy part of the cartilage is removed and the chondrocytes are separated after enzymatic digestion. The chondrocytes are proliferated and since an adequate number of cells, according to the extent of injury,is accomplished they are implanted into the damaged area, in a three dimensional sticky gel of fibrin glue. This method is applied in the cases of cartilage loss of over 8cm2. The cartilage reconstruction is applied in all genders, age 15-60 years old, for damaged areas ranging from 1.6 to 12 cm2 and in athletes of all sports. Autologous chondroblasts or chondrocytes preparation treatments are compliant with current European Parliament and of the council of 13 November 2007 on: Advanced therapy medicinal products and amending Directive 2001/83/EC and Regulation (EC) No 726/20042.

6.
BONE MARROW STEM CELLS
Bone marrow is the part of the body that produces the hematopoietic stem cells and all the mature blood elements. Bone marrow transplantation is a medical procedure whose aim is to replace unhealthy blood stem cells with healthy in order to treat a blood malignant or inherited disease. Bone marrow is selected by iliac crest aspiration and is a rich source of hematopoietic and mesenchymal stem cells. This product is also used to treat solid malignant tumors or in regenerative medicine.

7.
DENTAL PULP MESENCHYMAL STEM CELLS
Mesenchymal stem cells from the dental pulp have the potential to differentiate into a variety of cell types. Several publications show their importance in tissue regeneration due to the unique characteristics they demonstrated such as proliferation, differentiation and plasticity. Advantages
Easy & noninvasive procedure Safe cryopreservation Extensive proliferation Long lifespan Highly efficient Maximum regenerative ability

The interaction of mesenchymal stem cells with PRP is a promising tool for their therapeutic use in: Regenerative dentistry
Regenerative medicine of

many organs like:

Nerve & spinal cord Brain Heart Liver Bone Ligaments

Biohellenika developed an innovative technology to bank stem cells from teeth for people to secure their future health.
Chlidren Deciduous teeth Adults Wisdom teeth

and cartilage
Muscle Skin

Mesenchymal Stem Cells

8.
STEM CELLS FROM UMBILICAL CORD-PLACENTA UNIT
1. Hematopoietic Umbilical Cord BloodStem Cells. After the ligation of the umbilical cord the placenta blood is collected in a blood bag via umbilical cord vein puncture. Cord blood contains hematopoietic stem cells with clinical applications for malignant diseases for more than 20 years. Over 25.000 cord blood hematopoietic stem cells transplantations have been referred until today. 2. Hematopoietic Stem Cells from Placenta Drainage. Many stem cells remain in the placenta after puncturing the umbilical vein, because of the structure of the placenta and the conditions of birth. In the emergencies during the delivery, the volume of the collected placenta blood is low and the drainage of the placenta offers double amount of cells. The stem cells that are collected by this procedure are enriched in hematopoietic stem sells, endothelia and pro endothelia cells and are proposed to improve angiogenesis after coronary artery occlusion. The cell therapies are safe because the cells are autologous (patient's own cells), the procedures are minimally invasive with short recovery time for the patient but with long-lasting results. 3. Wharton's Jelly Mesenchymal Cells. Biohellenika developed a patent for a unique and innovative method to collect mesenchymal stem cells from the umbilical cord. The main advantages of this method are the highest number of collected cells, the cost effectiveness and a ready to use product. The cells are collected from the total length of the cord and cryopreserved in more than of one vials, so there is no need to proliferate and the cells maintain the stemness and all biological activities during the storage.

GREECE
THESSALONIKI
65, Georgikis Scholis Avenue, 57001 Thessaloniki ZEDA building T: 0030 2310.474.282, 2310.474.284 F: 0030 2310.474.285 Mobile: 0030 6944.677.746 E-mail: info@biohellenika.gr

INTERNATIONAL OFFICES
PATRA
Kos 18 & Panepistimiou 3 : 0030 2610.437.436 F: 0030 2610.431.339 M: 0030 6978.483.170

LARISSA
3 Megalou Alexandrou Str. 6th floor, 41222 T: 0030 2410.535.603 F; 0030 2410.535.664 M: 0030 6973.984.260

ROMANIA Bucharest Street Costache Negri No. 10A, District 5 T: 0040 213130063 F: 0040 765893583 0040 765893578 BULGARIA
Sofia 66 Exarch Joseph Str. T: 0700 10 804

ATHENS
2 Mesogion Str, Athens Tower, Building A T: 0030 210.77.08.882, 210.77.08.218 F: 0030 210.77.90.788 M: 0030 6973.346.704 email: info@biollenikasa.gr NATIONAL HELLENIC RESEARCH FOUNDATION (NHRF) 48 Vasileos Konstantinou Str. 11635 Athens : 0030 210.72.73.700 F: 0030 210.72.46.618

IRAKLION - CRETE
17 Amaltheias & Katechaki Str., 71201 : 0030 2810.229.351 M: 0030 6970.803.497

SERBIA
Belgrade Beo Stem ul. Balkanska 18, lokal 100 T: 0038 111.362.2383 M: 0038 162.758.688

CHANIA - CRETE
40-42 Tzanakaki Str. : 0030 2821058758 M: 0030 6976.977.384

SKOPJE Skopje
Frenklin Ruzvelt 4, lokal 5 1000, Skopje T: 0038 923.111.114 F: 0038 923.111.114

IOANNINA
M: 6970267540

ALEXANDROUPOLI
M: 6984604009

CYPRUS
Nicosia 55 Alekos Constantine Street T: 00357 22.028.208 F: 00357 22.028.210 Center Cyprus Phone: 00357 77.77.6001

SERRES
M: 6974197090

ITALY
Bel Paso Via Prima Retta Levante 237 CT T: 00393480695748

www.biohellenika.gr
OPEN LINE ___ 801.11. (Calls only from line phone)

99299

SCIENTIFIC CONSULTANTS K. Kouzi, MD PhD Professor Histology - Embryology, AUTh, Mobile: +30 6944677746 G. Koliakos, MD PhD Professor of Biochemistry, AUTh