Newstrategytoreverse signsofaging

SWSCC Educational Seminar & Suppliers Day August 23, 2012 - Las Colinas

questforeternallife immortality alchemistshuntedinvainfortherejuvenatingPhilosophersstone

Who is Unipex?

Chronological aging

Photoaging

SkinAging

Genetic

External/Internalfactors:
pollution,foods,alcohol,tobacco,emotions

Strategiestoreverseskinaging
Antiagingcream

SkinAging

Plasticsurgery

Makeupandphotoshop

Physiologicalchangeswithage

Youngskin Hyper orhypopigmentation Seborrheic keratoses Dry,scalyskin Increaseinskinfragility(barrierfunction) Increaseinroughness,wrinkles Laxity Easybruising Decreaseimmunefunction

Matureskin

AGING

Skinchangeswithage
Flaky Thick +++ SC Epidermis DEJ Dermis Hypodermis +++ Wavy +++

Stratumcorneum Epidermis

Compact

Thin Thickness =properskinbarrier Keratinocytes,Mc: proliferation,maturation Proteoglycanssynthesis

Dermal Epidermal junction

Dermis/epidermiscohesion

Flat

Dermislevel
+++ Low +++ ECMcomponent(collagens,elastin..) MMPsactivities Immunefunctions

Hypodermislevel
+++ Subcutaneousfat

AGING

Ourstrategytoreverseskinaging
Flaky Thick +++ SC Epidermis DEJ Dermis Hypodermis +/- Progerin +++ Wavy +++

Stratumcorneum Epidermis

Compact

Thickness =properskinbarrier Thin Keratinocytes,Mc,proliferation,maturation Proteoglycanssynthesis

Dermal Epidermal junction

Dermis/epidermiscohesion

Flat

Dermislevel
+++ Low +++ ECMcomponent(collagens,elastin..) MMPsactivities Immunefunctions

Hypodermislevel
+++ Subcutaneousfat +++ Progerin

AGING

Structuralagingduetosenescencemarker:progerin 1
Progerin asanewmarkerofaging Progerin isanalteredformoftheLamin Aprotein(nuclearintermediatefilamentprotein) Accumulationofprogerin innucleus OriginallyassociatedwiththeHutchinsonGilfordSyndrome(=Progeria) Progerin andaccelerationofaging Accumulation ofprogerin inducesvariousdefectsinnuclearstructureandfunctions Alterationscellcycleprogressioncausedbyprogerin knowntoacceleratehumanaging

Lamin A/C
From Cao et al. 2011

Thisprocessofaginggallopstoaboutseventimesthenormalrate

Progerin inhealthycells
The same molecular mechanisms responsible for premature aging in progeria act at low level in healthy cells

Progerin is expressed in healthy cells (fibroblasts and terminally differentiated keratinocytes mainly) and
accumulated with age (3% per year)

Cell nuclei from old individuals acquire defects similar to those of progeria patient cells

From Scaffidi et al. 2008

9yearsold

96yearsold

Progeria

Progerin hasbeenlinkedtophysiologicalageingaspresenceofprogerin isconcomitantwithalterations ofthedermalcompartment suchasdisorganizedconnectivetissue

TT2 abiomimeticstructuralpeptide
dermal structure and connective tissue organization TT2 (INCI: F3Acetyl Tripeptide2) : 3 amino acid peptide derived biomimetic of Elafin, an enzyme inhibitor

Focus on biomimetic peptide derived from structural proteins and enzymes implicated in the maintenance of

Protocol Maturehumandermisfibroblastswereincubatedfor96hourswithTT2peptide TheprogerinwasquantifiedusinganELISAmethodology.Resultsaregiveninpgofprogerinpergofproteins

22%inhibition progerin production

TT2peptide

TT2peptide decreasessignificantlytheproductionofprogerin, attestingitsefficacyonthesenescenceprocess

EffectofTT2peptide ondermalstructureand connectivetissueorganization

MMP1,MMP3&MMP9inhibition
Syndecan Dermis contraction Elastase,collagenase MMPs
2peptide control TT Progeline TT2peptide control Progeline

+27%collagen contraction
Progerin T0 After24h

EffectofTT2peptide ondermalstructureand connectivetissueorganization

Control

+Elastase

TT2peptide +Elastase +Progeline

100% inhibition elastase

Syndecan Dermis contraction Elastase,collagenase MMPs
Control +collagenase TT2peptide +collagenase +Progeline Elastinfibers

Progerin
Collagenfibers

43% inhibition collagenase

EffectofTT2ondermalstructureandconnective tissueorganization

Bymodula ngsenescenceprocess(progerin),
Syndecan Dermis contraction Elastase,collagenase MMPs

TT2peptide inducesseveralbiological responsesatthedermis&epidermislevels

Progerin

13

ClinicalStudies
Firmness,Laxity

Clinicalevaluationofantisaggingeffectonjawlines 1
Protocol

10healthyvolunteers :5464yearsoldwithagedskin Formulation:2%TT2peptide emulsion creamorplacebo Application:splitface andsplitneck 2X/dayfor56days Measurementofthevolumeofthejawlines ofthefacebyinvivo fringeprojectionprofilometry
TT2peptide2%

80%ofvolunteers had animprovement

TT2peptide decreasessignificantlythevolumeofthejawlinesafter 28and56days

Clinicalevaluationoffirmness&elasticity Cutometry
Protocol

13healthyvolunteers(54 66yearsold)withsaggyskin(lossoffirmness,slackeningskin) Formulation:2%TT2peptideemulsioncreamorplacebo Application:splitface andsplitneck 2X/dayfor28days Measurementsareperformedonbothcheeks(diameterprobe=2mm)bycutometry

Improvement upto: 93%ofcutaneous elasticity 82%ofcutaneous firmness 42%ofcutaneous viscoelasticity

TT2peptide2%

TT2peptide increasessignificantlyfirmness,elasticityandviscoelasticity after28days

Clinicalevaluationoffirmness&elasticity
Majorbuildingeffect overtime! D0

TT2peptide

Slight improvement

Summary TT2peptideeffect
Firmness,Elasticity Laxity

Syndecan Dermis contraction Elastase,collagenases Progerine

Reverseeffect of TT2peptide

Ourstrategytoreverseskinaging
Flaky Thick +++ SC Epidermis DEJ Dermis Hypodermis +++ Wavy +++

1
Compact

Stratumcorneum Epidermis

Thickness =properskinbarrier Thin Keratinocytes,Mc,proliferation,maturation Proteoglycanssynthesis

Dermal Epidermal junction

Dermis/epidermiscohesion

Flat

Dermislevel
+++ Low +++ ECMcomponent(collagens,elastin..) MMPsactivities Immunefunctions

Hypodermislevel
+++ Subcutaneousfat

Structural agingduetolipoatrophy
Youngskin
Fullfatcompartments

Matureskin
Deflatedfatcompartments

Nasolabial wrinkles

Hormesis concept

Hormesis : homeostatic and adaptative beneficial response of cells to microstress

exposure in order to correct a physiological imbalance

The word hormesis comes from the greek root hormeticos, which means
stimulation and the first mention of the term hormesis in the open scientific literature was published in 1943

+ + +

Concept launched by Givenchy with Vaxin (2010) :

This vaccinelike serum is formulated with a unique complex capable of generating microstimuli, which encourage the skin's cells to produce their own agedefying protein HSP70

Lipohormesis Newproprietarypatentedconcept 2
Lipohormesis = hormesis applied to adipose tissue Various stresses in the adipose tissue are able to stimulate its expansion through the
activation of Hypoxia Inducible Factor (HIF1), a transcription factor
Matureskin +

HIF1
Adipogenesis activation
(adipocytedifferenciation)

Activationof adipocyteanabolism
Lipolysis Inhibition
(freefattyacidrelease)

Lipogenesis activation
(triglyceridesynthesis)

Youngskin
Adiposetissuegrowth

Reverseaging Wrinklereduction Plumperskin

L.O. Alipofillinglikealternative
LO(INCI:Ornithine(and)Phospholipids(and)Glycolipids):Lornithine,anaminoacid
obtainedbybiotechnologyfromvegetablestarch,encapsulatedinaIonosome

Ionosome: new generation of delivery system (proprietary patented). Polysaccharidecoated encapsulation: polysaccharides (stearoyl inulin) inserted in the
phospholipid membrane = protective coating

Ionosome benefits: Resistantdeliverysystem Highpenetrationcapacitytotargetadipocytes Higherefficacy

L-ornithine
Lipophilic side C18 C18 Hydrophilic side

C18 C18

L O

EffectofLOontheactivationofHIF1and adipocyteanabolism
Protocol Human preadipocytes were isolated from biopsiesandcultivated fordifferentiation. 0.02%LO was added onadipocytesculture Genic expressionwas evaluated through cDNA methodology Results Adipofill
HIF1 genes involved inadipogenesis genes involved in lipolysis

genes involved inlipogenesis

Adipocyte differenciation

Triglyceride synthesis

Freefatty acid release

LOreproduceslipohormesis phenomenonandmimics lipofilling result byincreasing fatty tissuevolumewith aglobalaction

Lipidstorageenhancement lipolysis
Protocol

Humanadipocyteobtainedfromapieceofsurgicalresectionwereincubatedwith0.05%and0.2%LO Free fatty acids (NEFA) were quantified in the media using a spectrophotometric method and the adipocyte size was evaluated through the measurement of their average diameter after staining with red oil (specific for lipids)

LO

Increase insize LO Increase inlipid content

LOinhibitslipolysisanddecreasesthereleaseoffattyacidsfrom adipocytesbyincreasinglipidstorage

ClinicalStudies
Nasogenian fold

Reductionofnasogenian fold

Protocol 20healthyvolunteers(40 60yearsold)withnasogenian folds. Formulation:2%LOemulsioncreamorplacebo Application:splitfaceandsplitneck2X/dayfor56days Measurementofdepth androughness byprofilometry andevaluationofsmoothness byadermatologist

Reduction upto: 27.5%ofroughnesss 37.6%ofwrinkle depth

85% of volunteers had an improvement

LO2% LO2%

LOstronglyreducestheappearanceofnasogenian foldafter onlyonemonth

Reductionofnasogenian fold

D0

D60

LOstronglyreducestheappearanceofnasogenian fold

Summary LOeffect

Nasogenian fold

HIF1 Adipogenesis Lipogenesis Lipolysis Adiposetissue

Reverseeffect of L O

1
TT2peptide
Bymodula ngsenescenceprocess(progerin), TT2peptide targetsDERMIS restructurationfora remodellingeffectandwrinklesreduction

2
LO
Byinducinglipohormesis(HIF1), LO targetsHYPODERMIS restructurationfora lipofilling effectandnasogenian foldreduction

+ 2

LaSpiritCream
forasynergisticefficacy

LaSpiritCream
UNIQUE, SOPHISTICATED AND BIOEFFICIENT ACTIVE INGREDIENTS AND DELIVERY SYSTEMS
TT2peptide:Improves firmness &reduces wrinkles andsagging LO: Reversesagerelated lipoatrophy toplump theskin

DELICIOUS TEXTURES THAT DELIVER EXQUISIT SKIN FEEL

Hydrogenated Lecithin (and)C1216Alcohols (and)Palmitic acid:Brings the phospholipids touch (coolness,softness)&comfort SodiumAcrylatesCopolymer (and)Lecithin:Brings alighttexturetothe emulsion

LaSpiritCream
Tested by62women (45to70)twice aday for28day SKINBENEFITS Theproductispleasant toapply Thetextureissmooth Theskinfeelssofter Theskinfeelssuppler Theskinseemshydrated Theskinismoreluminous Theskinlooksmoreuniform Thesignsoffatigue arelessvisible Theskintexture looksimproved Theskinfeelsfirmer Theskinhaveahealthyglow Theskinlooksvisiblysmooth out AFTER 1WEEK 100% 96% 92% 86% 85% 71% 67% 69% 67% 63% 56% 51% AFTER 4WEEKS 100% 97% 97% 91% 87% 73% 79% 75% 75% 73% 72% 70% 87% 89% 84% 73% 77% Compared toIris historical result on antiaging consumer tests2567tests IRISDATABASE*

Conclusion Focusonstructuralaging
Cellularagingwithaprogressivedysfunctionofthecellmetabolism(progerin) Cutaneous aging characterized by a loss of elasticity, appearance of wrinkles Structural aging due to a lipoatrophy (loss of adipose tissue)

Reverseeffect of TT2peptide&LO YoungSkin MatureSkin

ThankYou!

JeanneCalment (1875 1997) Livingtotheageof122years=44724days