*Address for 6orrespondence:

8ama Venkatesh, 0. Pu||a Reddy Co||ege ol P|arracy, Ve|d|palrar,

lyderaoad- 500 028, Ard|ra Prades|, lrd|a.
E-ma||: ver|ales|sara_|olra||.cor
T|e aqueous el|aro||c exlracl ol Panoanus ooorar|ss|mus (Pardaraceae) rool Was lesled lor |ls ellecl or o|ood g|ucose |eve|s |r rorra| ard d|aoel|c rals.
lypog|ycer|a Was ooserved |r oasa| cord|l|ors W|er lesled al ar ora| dose ol Z5, 150 ard 300 rg/|g oody We|g|l. T|e el|aro||c exlracl |as d|sp|ayed a
s|gr|l|carl dose-deperderl arl||yperg|ycer|c acl|v|ly |r ora| g|ucose lo|erarce lesl ard a|so lourd lo reduce l|e |rcreased o|ood g|ucose |r a||oxar-|rduced
d|aoel|c rals (3Z al 150 rg/|g ard 51 al 300 rg/|g oody We|g|l). C|ror|c adr|r|slral|or (10 days) ol l|e el|aro||c exlracl ol rool s|gr|l|carl|y reduced l|e
o|ood g|ucose |r a||oxar-|rduced d|aoel|c rals. T|e exlracl Was a|so lourd lo reduce l|e |rcreased o|ood urea, |r||o|l l|e oody We|g|l reducl|or ard |eucoper|a
|rduced oy a||oxar adr|r|slral|or. T|e el|aro||c exlracl Was lourd lo ellecl|ve|y scaverge l|e 0PPl ard ||p|d perox|de lree rad|ca|s |n v|rro W|l| ar lC va|ue ol
50
10 ard 8 g/r|, respecl|ve|y. T|e pre||r|rary p|yloc|er|ca| exar|ral|or revea|s l|e preserce ol l|avaro|ds ard larr|rs, W||c| ray oe allr|ouled lo ooserved
arl|ox|darl ard s|gr|l|carl arl||yperg|ycer|c properl|es.
Keywords: Panoanus ooorar|ss|mus, a||oxar, g|ucose lo|erarce, d|aoeles, rools, arl||yperg|ycer|a, arl|ox|darl acl|v|ly.

Pevisec: 17/08/2012 3cbuittec: 18/11/2011 Acceptec: 00/0/2012

|NTR00U6T|0N
Diabetes mellitus is chronic metabolic disorder characterized
by abnormalities in carbohydrate, Iat, protein metabolism and
appropriate hyperglycemia resulting Irom deIects in insulin
1
secretion or peripheral insulin resistance . In India, diabetes
has been known Ior ancient ages and sweetness oI the diabetic
urine was mentioned by Sushruta in Ayurveda and use oI plant
extract based on traditional knowledge is common practice.
Available ethanobotanical inIormation reports about 800
2
plants which may possess antidiabetic potential . However,
most orally active hypoglycemic remedies extracted Irom
plant material are not scientiIically evaluated, incompletely
characterized. Although insulin therapy and oral
hypoglycemic agents is the mainstay oI treatment oI diabetes
and are eIIective in controlling hyperglycemia, they have
prominent side eIIects and Iailed to signiIicantly alter the
3
course oI diabetic complications . ThereIore, the search Ior
more eIIective and saIer hypoglycemic agents has continued
to be an important area oI active research.
Pandanus odoratissimus Linn. (Pandanaceae) is a much
branched shrub or a small tree with many aerial stilt roots.
This plant is popularly known as 'Screw pine Tree' and locally
known as 'Mogili'. The plant distributed through out India,
commonly Iound along the seacoast, river banks, ponds and
4,5
near to water streams . In traditional system oI medicine the
6,7
roots are claimed to be useIul in treating diabetes . The leaves
Ant|d|abet|c Act|v|ty of Pandanus odoratissimus Root Extract
1 1 1 1 2
8ama Venkatesh* , Kusuma R , 8ateesh V , Hadhava Reddy and Ramesh Hu||ang|
1
0. Pu||a Reddy Co||ege ol P|arracy, Ve|d|palrar, lyderaoad - 500 028, lrd|a.
2
Juo||arl lrroval|or, 9, lrduslr|a| 3uouro, Yes|Warl|pur, 8arga|ore-5 022, lrd|a
Ia4|ea Iecrae| e| P|ermecec||ce| I4cce||ea ea4 keseerc| Assec|e||ea e| P|ermecec||ce| Ieec|ers e| Ia4|e
and Ilowers oI P. odoratissimus are reported to be useIul in
8
leucoderma, tumours, leprosy and skin diseases In India and
Burma the male Ilowers are valued Ior Iragrance and used as a
hair decoration. Physcion, cirsilineol, n-triacontanol,
sitosterol, camphosterol, daucosterol and palmitic acid,
9
stearic acid in rhizomes has been reported . To the best oI our
knowledge no report is available on antidiabetic eIIects oI P.
odoratissimus roots. The present study was undertaken to
veriIy the claim and evaluate the antidiabetic property oI P.
odoratissimus roots with the aim oI developing a natural
antidiabetic drug.
HATER|AL8 AN0 HETh008
P|ant mater|a|
P. odoratissimus roots were collected Iresh Irom Bethavolue
Village, Nalgonda district, Andhra Pradesh, India. The
botanical identiIication oI plant was perIormed by ProI.
Ramakrishna, Head, Department oI Botany, P.G College oI
Sciences, Osmania University, Hyderabad. Voucher
specimen (PNO-308-9) was deposited in Department oI
Pharmacognosy and Phytochemistry oI G. Pulla Reddy
College oI Pharmacy, Hyderabad, India. The roots were shade
dried and grounded by electric mill and passed through mesh
# 60.
Preparation of extract
The dried root powder (850 g) was extracted at room
0
temperature (25-30 C) with 80 aqueous ethyl alcohol Ior 7
days with occasional shaking Iollowed by re-maceration with
the same solvent Ior 5 more days. The macerates were
combined, Iiltered, and distilled oII in reduced pressure. The
resulting concentrate was vacuum dried at 40C to yield the
lrd J P|arr Edu Res, 0cl-0ec, 2012/ Vo| 4o/ lssue 4 340
dry extract (yield:3.03 w/w). The dry extract was kept in a
vacuum desiccator until use. The resultant organic extract was
tested qualitatively Ior the presence oI various
10, 11
phytoconstituents using standard procedures .
Animals
Male Wistar rats (160-180 g) were used Ior the study. Animals
were Ied with standard diet (National Institute oI Nutrition,
Hyderabad) and water ad libitum during quarantine period.
Animals described as Iasted had been allowed Iree access to
water. The animal experimentation was carried out according
to the Committee Ior the Purpose oI Control and Supervision
oI Experimentation on Animals (CPCSEA) guidelines and
Institutional Animal Ethics Committee approved all the
procedures Ior investigation. The aqueous ethanolic extract oI
P. odoratissimus root (POE) was administered at diIIerent
doses as a Iine suspension using 0.5 (w/v) aqueous carboxy
methyl cellulose (CMC).
Acute toxicity
To determine the acute toxicity, a single oral administration oI
the POE at diIIerent doses (0.5, 1, 2, 3.0 g/kg body weight)
12
was administered to diIIerent groups oI mice . Each group
consists oI six animals. Control group received the vehicle.
The animals were observed continuously Ior the initial period
oI 2 h, intermittently Ior the next 6 h and then 24 h and 48 h
Iollowing oral administration oI diIIerent doses oI drug
administration Ior death and abnormality in behavioral
changes.
Effect of P. odoratissimus extract on blood glucose levels of
normal fasted rats
Fasted rats were divided into 4 groups oI 6 rats per group. The
Iirst group oI animals are given 0.5 w/v aqueous CMC
through oral route and served as control. Group II-IV received
CMC suspension oI POE at dose oI 75, 150 and 300 mg/kg,
respectively. Blood samples were collected Irom retro orbital
under light ether anesthesia just prior to and at 1, 2 and 3h
Iollowing oral administration oI extract. Plasma was
separated and blood glucose levels were assessed Ior
biological changes. Glucose levels were estimated by using
commercially available glucose kit (Autospan, Span
13
diagnostics, India) based on glucose-oxidase method and
absorbance was measured at 505 nm.
Effect of P. odoratissimus extract on glucose tolerance in

rats
Fasted rats were divided into 4 groups oI 6 rats in each group.
Control rats were given 0.5 aqueous CMC (group I). CMC
suspension oI POE (75, 150, and 300 mg/kg) was
administered orally to II, III and IV group oI rats. The rats oI
all the groups were given glucose (2 g/kg, orally) 30 min aIter
administration oI the extract. Blood samples were collected
Irom the retro orbital just prior to glucose administration and
14
at 30, 90 and 120 min aIter glucose loading . The blood
glucose levels were measured immediately by glucose-
13
oxidase method .
Effect of the P. odoratissimus extract on alloxan induced
diabetic rats
Male Wistar rats were made diabetic by a single intra
peritoneal injection oI 120 mg/kg body weight oI alloxan
monohydrate in sterile normal saline. Five days later blood
samples were drawn and glucose levels were determined to
conIirm the development oI diabetes (~ 250 mg /dl). The
diabetic rats were divided into 3 groups, each containing 6
animals. Control rats (group I) were given 0.5 w/v aqueous
CMC orally, while POE at dose oI 150 and 300 mg/kg body
weight were given to the II and III groups. Blood samples
were collected just prior to and at 1, 3 and 5 h aIter extract
administration and plasma glucose levels were measuredwere
13
measured immediately .
The action oI POE was also tested Ior longer duration oI
treatment. The male Wistar rats were divided into 4 groups oI
10 rats each. Group I served as diabetic control group,
received vehicle 0.5 aqueous CMC. The rats oI group II and
III received two diIIerent concentrations oI POE (150 and 300
mg/kg) Ior 5 days. ThereaIter all rats oI the diabetes control
and treated groups (groups I, II and III) were injected with
alloxan monohydrate (120 mg/kg, i.p.). Group IV animals
15
served as the normal and received vehicle .
The administration oI test extract was continued Ior 10 more
days aIter alloxan treatment. Blood samples collected through
the retro orbital puncture just prior to and on days 5 and 10
aIter the alloxan injection. The Iollowing parameters were
measured:
1. Blood sugar 2. Blood urea 3. Body weight and 4. Total
leukocyte count.
Antioxidant studies
The Iree radical scavenging activity oI POE was measured by
using stable DPPH (2,2-diphenyl-1- picryl hydrazyl) as
16
described by Aquino et al . DPPH in its radical Iorm has an
absorption peak at 517 nm, which disappears on reduction by
an antioxidant compound. 1 ml oI POE in diIIerent
concentrations (10-100 g) was added to 2 ml oI Ireshly
prepared methanolic solution oI DPPH (90 M) and the
volume was made up to 4 ml with methanol. Absorbance was
measured at 517nm, aIter 1 h. Curcumin served as a standard.
The percentage inhibition oI DPPH in the reaction medium
was calculated by comparing with control.
lrd J P|arr Edu Res, 0cl-0ec, 2012/ Vo| 4o/ lssue 4 341
3ara ver|ales| er a| Arl|d|aoel|c Acl|v|ly ol Panoanus ooorar|ss|mus Rool Exlracl
Rat liver homogenate was used Ior determining the extent oI
lipid peroxidation. The lipid peroxide Iormed was measured
by thiobarbituric acid reacting substance (TBARS) Iormation
17
as described by Ohkawa et al . Reaction mixture (4 ml)
containing 0.5 ml oI rat liver homogenate in Tris-HCl buIIer
H
(40 mM, p -7), 3.2 ml oI diIIerent concentrations oI test
substances(10-100 g/ml) and 100 l oI each oI 0.15 M KCl,
15 mM oI Ierrous sulphate and 6 mM ascorbic acid was
0
added and incubated at 37 C Ior 1 h. To the incubation mixture
1ml oI 10 TCA (Trichloro acetic acid) was added and a
0
sample was centriIuged at 3000 rpm Ior 20 min at 4 C. 1 ml oI
0.8 TBA (thibarbituric acid) was added to the supernatant
0
solution and the mixture is heated at 100 C Ior 20 min in water
bath. AIter cooling the colored TBA MDA complex was
extracted with 2 ml butanol and absorbance oI pink
chromophore was measured at 532 nm. Butyl hydroxyl
toluene served as a standard. The percentage inhibition oI
lipid peroxidation was determined by comparing the results oI
test compound with those oI control not treated with extract.
Statistical analysis
All values were expressed as mean + SEM. Results were
analyzed statistically by using analysis oI variance (ANOVA)
Iollowed by Dunnett's test. Values oI P0.05 were considered
signiIicant.
RE8ULT8
In oral acute toxicity test, no mortality and abnormal
behavioural changes were observed in mice up to a dose oI 3
g/kg body weight. Further the antihyperglycemic activity was
carried out at an oral dose oI 75, 150 and 300 mg/kg.
Administration oI POE was Iound to reduce blood sugar
levels signiIicantly in normal rats; however the reduction was
marginal and not severe. The maximum reduction in blood
glucose was noted at 3 h aIter the administration oI extract.
The reduction was 13.09 at a maximum dose oI 300 mg/kg
(Table 1).
The eIIect oI POE on glucose tolerance is given in Table 2. A
sharp increase in glucose concentration was observed in
control rats at 60 min aIter glucose load. The signiIicant
(P0.001) glucose tolerance was observed with all test dose
levels at 60 min aIter glucose loading (90 min aIter drug
dosing), however the tolerance was decreased at 2 h. The
maximum glucose tolerance was noted Ior rats administered
with 300 mg/kg oI extract. The decreased glucose levels in
comparison to control rats are 24.77, 41.66 and 54.31 oI 75,
150 and 300 mg/kg oI extract, respectively at 60 min aIter
glucose charge.
Administration oI P. odoratissimus root extract was Iound to
reduce blood glucose levels in alloxan induced diabetes rats
Croup Treatment P|asma g|ucose |eve|s (mg|d|}
(0ose| kg body we|ght} |n|t|a| 1 h 2 h 3 h
l Corlro| -urlrealed Z9.8 0.98 Z.ZZ 1.05 ZZ.8 1.02 ZZ.1 0.85
ll El|aro||c exl., Z5 rg ZZ.12 0.Z1 Z3.18 0.85 Z2.2 0.92 Z1.Z2 0.92
(1.Z) (.Z3) (3.Z)
lll El|aro||c exl., 150 rg 81.0 0.98 Z1.53 0.5 9.51 0.81 8.Z0 0.0
(3.21) (10.Z2) (11.51)
lv El|aro||c exl., 300 rg 80. 0.91 Z0.Z3 0.91 Z1.9 0.81 Z.1Z 0.8
(Z.8) (Z.92) (13.09)
Tab|e 1: Effect of P. odoratissimus root extract on b|ood g|ucose |eve|s |n fasted norma| rats
a
va|ues are rear 3.E.V., n=, P < 0.05, P < 0.01 vs Corlro|. F|gures |r parerl|es|s |rd|cale l|e percerlage
decrease |r p|asra g|ucose |eve|s
Croup Treatment P|asma g|ucose |eve|s (mg|d|}
(0ose| kg body we|ght} |n|t|a| 30 m|n 60 m|n 120 m|n
l Corlro|- g|ucose, 2g Z9.10 1.11 108.1Z 1.1Z 193.32 1.32 108.12 1.0Z
ll El|aro||c exl. Z5rgg|ucose 2g 8Z.11 1.31 95.81 1.25 115.13 1.33 100.Z0 1.23
(11.8) (21.ZZ) (.8)
lll El|aro||c exl. 150rgg|ucose 2g ZZ.Z1 1.32 110.13 1.18 112.ZZ 1.30 105.01 1.10
(11.) (2.81)
lv El|aro||c exl. 300rgg|ucose 2g 9.9 1.19 101.1 1.29 88.32 1.13 98.99 1.15
(.32) (51.31) (8.11)
a
Tab|e 2: Effect of P. odorat|ss|mus root extract on ora| g|ucose to|erance test |n rats
a
va|ues are rear 3.E.V., n=, P < 0.05, P < 0.01 vs Corlro|. F|gures |r parerl|es|s |rd|cale l|e percerlage
decrease |r p|asra g|ucose |eve|s
3ara ver|ales| er a| Arl|d|aoel|c Acl|v|ly ol Panoanus ooorar|ss|mus Rool Exlracl
lrd J P|arr Edu Res, 0cl-0ec, 2012/ Vo| 4o/ lssue 4 342
197 mg/dl. The extract has produced a dose dependent
antihyperglycemic property. Treatment with POE
signiIicantly reduced the serum glucose levels by 9.03 and
25.03 respectively oI 150 and 300 mg/kg oI extract on day
10. The decreased blood glucose level Irom 340 to 263 mg/dl
in diabetic rats is observed indicating the pancreatic tissue
gets repaired by itselI aIter a single alloxan injection. The
repaired was Iound to be much Iaster in animals received P.
odoratissimus extract (Table 4).
A decrease in rat body weight (p 0.001) was noted in alloxan
induced diabetic rat but when the animals were treated with P.
odoratissimus extract, the decrease in body weight was
completely suppressed and recovery oI body weight was
observed in the animals received POE with the dose oI 300
mg/kg body weight (Table 5). In diabetic animals the normal
Iunction oI the kidney is assessed as blood urea levels and is
distributed (30.86 in control versus 69.50 mg/dl in diabetic
rats) in diabetic rats 5 days aIter oI alloxan administration.
(Table 3). The Iasting blood glucose levels in alloxan induced
diabetes rats were 262 298 mg/dl. There was no signiIicant
diIIerence in blood glucose levels oI diabetic control.
However the administration oI POE has shown a signiIicant
dose dependent Iall in blood glucose levels. The
antihyperglycemic eIIects were observed Irom 1 h aIter drug
administration and the activity was Iound to be maximum at 5
h oI drug administration. The decreased blood glucose levels
in diabetic rats at 5 h aIter the administration oI root extract is
37.81 and 51.44 oI 150 and 300 mg/kg extract.
Sub-chronic administration oI P. odoratissimus root extract
was Iound to reduce blood glucose levels in alloxan induced
diabetic rats. The plasma glucose levels were markedly raised
(up to 4.75 times) in the diabetic control as compared with the
normal control on day 5 aIter the alloxan injection. The
maximum reduction in blood glucose levels is observed with
animals administered with 300 mg/kg on day 5, with percent
protection oI 37.73. Continuous administration oI POE
signiIicantly (P0.001) reduced the blood glucose levels to
Croup Treatment P|asma g|ucose |eve|s (mg|d|}
(0ose| kg body we|ght} |n|t|a| 1 h 3 h 5 h
l 0|aoel|c corlro| 290.05 5.19 283.8 5.Z8 293.81 3.18 298.01 1.10
ll El|aro||c exl., 150rg 2Z5.50 5.82 223.15 1.88 20.Z2 1.08 185.31 3.93
(21.23) (29.1) ( 3Z.81)
lll El|aro||c exl., 300 rg 22.10 1.Z9 251.9 5.91 180.0 3.53 119.Z2 3.5Z
(11.2Z) (38.Z1) ( 51.11 )
a
Tab|e 3: Effect of P. odorat|ss|mus root extract on a||oxan- |nduced d|abet|c rats
a
va|ues are rear 3.E.V., n=, P < 0.05, P < 0.01 vs Corlro|. F|gures |r parerl|es|s |rd|cale l|e percerlage
decrease |r p|asra g|ucose |eve|s
Croup Treatment P|asma g|ucose |eve|s (mg|d|}
(0ose| kg body we|ght} |n|t|a| 0ay 5 0ay 10
l 0|aoel|c corlro|-a||oxar 120rg Z2.3Z 1.1 312.9Z 1.23 23.88 2.3
ll El|aro||c exl. 150rga||oxar 120rg Z9.12 1.2Z 29.8 3.03 212.00 5.99
(15.53) (9.039)
lll El|aro||c exl. 300rga||oxar 120rg Z8.9 1.35 213.Z5 3.91 19Z.30 5.23
(3Z.Z3) (25.03)
lv Norra| Z.5 1.0 2Z5.1 1.3 2Z1.Z2 1.13
a
va|ues are rear 3.E.V., n=, P < 0.05, P < 0.01 vs Corlro|. F|gures |r parerl|es|s |rd|cale l|e percerlage
decrease |r p|asra g|ucose |eve|s
Tab|e 4: Effect of chron|c adm|n|strat|on of P. odoratissimus root extract on b|ood g|ucose |eve| |n rats
treated w|th a|oxan
Croup Treatment ody we|ght (g}
(0ose| kg body we|ght} |n|t|a| 0ay 5 0ay 10
l 0|aoel|c Corlro| - a||oxar 120 rg 10 1.1Z 130 3.5 120 2.21
ll El|aro||c exl. 150 rg a||oxar 120 rg 15 1.22 125 2.55 135 3.101
lll El|aro||c exl. 300 rg a||oxar 120 rg 13 2.11 115 3.52 111 3.0Z
lv Norra| 1Z 1.11 18 3.12 189 1.91
a
va|ues are rear 3.E.V., n=, P < 0.05, P < 0.01 vs Corlro|.
a
Tab|e 5: Effect of P. odorat|ss|mus root extract on body we|ght |n rats treated w|th a||oxan
3ara ver|ales| er a| Arl|d|aoel|c Acl|v|ly ol Panoanus ooorar|ss|mus Rool Exlracl
lrd J P|arr Edu Res, 0cl-0ec, 2012/ Vo| 4o/ lssue 4 343
Animals treated with P. odoratissimus extract along with
alloxan had lower (P~ 0.001) blood urea levels signiIicantly.
The maximum reduction in urea level is noted with the
animals received the POE at the dose oI 300 mg/kg with
percent reduction oI 32.26 (Table 6).
EIIect oI P. odoratissimus extract on total white blood cells
(WBC) count in alloxan treated animals is shown in (Table 7).
Total WBC was signiIicantly reduced Irom 7434 to 5263,
Iive days aIter administration oI alloxan indicating cellular
injury brought about by alloxan. Administration oI POE
signiIicantly prevented alloxan induced cellular damage at
both test dose levels, as seen Irom the number oI total WBC.
The Iree radical DPPH was eIIectively scavenged by POE.
The radical scavenging capacity oI test extract and standard
curcumin is increased with increasing concentration (Fig.1).
The IC was Iound to be 13 g/ml whereas standard curcumin
50
shows 10 g/ml. POE was Iound to inhibit in vitro tissue lipid
peroxidation in a concentration dependent manner ranging
Irom 10-100 g/ml. Fig. 2 represent, the inhibition oI lipid
peroxidation activity. IC value oI POE is 8 g/ml. The butyl
50
hydroxyl toluene (BHT) was used to compare the antioxidant
potential, and the IC oI BHT (6 g/ml) was comparable with
50
that oI POE.
0|8U88|0N
The results oI present study indicates that POE was Iound to
Croup Treatment |ood urea (mg|d|}
(0ose| kg body we|ght} |n|t|a| 0ay 5 0ay 10
l 0|aoel|c Corlro|-a||oxar 120rg 30.8 0.38 9.50 0.33 12.3Z 0.1
ll El|aro||c exl. 150rga||oxar 120rg 2.1Z 0.29 0.Z0 0.1Z 31.9 0.31
(12.2) (1Z.18)
lll El|aro||c exl. 300rga||oxar 120rg 28.158 0.31 8.28 0.38 28.Z0 0.28
(1.Z5)(32.2)
lv Norra| 25.11 0.31 29.5 0.38 2Z.53 0.29
a
Tab|e 6: Effect P. odorat|ss|mus root extract on b|ood urea |n an|ma| treated w|th a||oxan
a
va|ues are rear 3.E.V., n=, P < 0.05, P < 0.01 vs Corlro|. F|gures |r parerl|es|s |rd|cale l|e percerlage
decrease |r p|asra g|ucose |eve|s
3
Croup Treatment Tota| |eukocyte counts (mm }
(0ose| kg body we|ght} |n|t|a| 0ay 5 0ay 10
l 0|aoel|c Corlro| - a||oxar 120rg Z131 32 523 129 533Z 113
ll El|aro||c exl. 150 rg a||oxar 120rg ZZ0 35Z 5Z0 399 Z15Z 153
lll El|aro||c exl. 300 rg a||oxar 120rg 8910 193 Z29 520 8310 333
lv Norra| 8Z32 10 9018 32 910 310
a
Tab|e 7: Effect of P. odorat|ss|mus root extract on tota| |eukocyte counts |n rats treated w|th a||oxan
a
va|ues are rear 3.E.V., n=, P < 0.05, P < 0.01 vs Corlro|. F|gures |r parerl|es|s |rd|cale l|e percerlage
decrease |r p|asra g|ucose |eve|s
F|g. 1: 8caveng|ng effects on 0PPh rad|ca| of P. odoratissimus
root extract
F|g. 2: Effects of P. odorat|ss|mus root on |nh|b|t|on of ||p|d
perox|dat|on
3ara ver|ales| er a| Arl|d|aoel|c Acl|v|ly ol Panoanus ooorar|ss|mus Rool Exlracl
lrd J P|arr Edu Res, 0cl-0ec, 2012/ Vo| 4o/ lssue 4 344
reduce the glucose levels in normal, glucose loaded animals
and in animals made diabetic with alloxan. Alloxan has been
shown to induce Iree radical production and cause tissue
18
injury . The pancreas is especially susceptible to the action oI
alloxan induced Iree radical damage. The present Iindings
indicate that P. odoratissimus can act as Iree radical scavenger
in vitro in both DPPH and lipid peroxides and ameliorate the
destruction oI WBC and conIirms the possibility that the
major Iunction oI the extract is on the protection oI vital
tissues including the pancreas, thereby reducing the causation
oI diabetes in these animals. Other possible mechanism
includes the stimulation oI -cells and subsequent release oI
insulin and activation oI the insulin receptors. Estimation oI
insulin levels may give more insight into the mechanism oI
the antidiabetic activity shown by the extract.
The present study also indicates that P. odoratissimus can
partially inhibit alloxan renal toxicity as seen Irom the blood
urea levels. The preliminary phytochemical investigation
results indicate the presence oI tannins, steroids, triterpenoids
and Ilavonoids and/or their glycosides in POE. Flavonoids
and tannins are well-known polyphenolic natural
antioxidants, which may be responsible Ior the antioxidant
role oI P. odoratissimus and Ior observed antihyperglycemic
properties. The active ingredient in the extract which reduces
the blood sugar is not known at present.
A6KN0wLE0CEHENT8
The authors wish to thank management oI the college Ior
providing research Iacilities.
REFEREN6E8
1. V|s|ra VK, Parda 88, 0|os| 0, V|s|ra 3K, 8ar|| 88. Ererg|rg
lrerds |r l|e raragererl ol d|aoeles: ar overv|eW. lrd|ar 0rugs 2008,
15: 111-.
2. A|arcor-Agu||ar FJ, Rorar-Raras R, Perez-0ul|errez 3,
Agu||ar-Corlreras A, Corlreras -weoer CC , F|ores -3aerz JL. 3ludy
ol l|e arl|-|yperg|ycer|c ellecl ol p|arls used as arl|-d|aoel|cs. J
El|arop|arraco| 1998, 1: 101-10.
3. Rarg lP, 0a|e VV, R|ller JV, F|oWer RJ. Rarg ard 0a|e's
l|
P|arraco|ogy. ed. P|||ade|p||a: C|urc|||| L|v|rgslore E|sev|er,
200Z, 39Z-9.
1. Aroryrous. T|e wea|l| ol lrd|a, A 0|cl|orary ol lrd|ar RaW Valer|a|
ard lrduslr|a| Producls. vo|. 3. NeW 0e|||: Puo||cal|r ard lrlorral|or
0|reclorale, Courc|| ol 3c|erl|l|c ard lrduslr|a| Researc|, 1959, p. 219-
21.
5. Aroryrous. T|e Ayurved|c P|arracope|a ol lrd|a. vo|. 1. NeW 0e|||:
Corlro||er ol Puo||cal|or, 0overrrerl ol lrd|a, 2005,9.
. Nad|arr| KV, Nad|arr| AK. (1995). lrd|ar Valer|a Ved|ca. vo|.1.
8oroay: Popu|ar Pra|as|ar, 1995, 891-5.
Z. Vad|ava C|elly K, 3|vaj| K, Tu|as| Rao K. F|oWer|rg p|arls ol C||lloor
0|slr|cl, Ard|ra Prades|, lrd|a. T|rupal||: 3luderls 0llsel Pr|rler, 2008,
38-9.
8. K|rl||ar KR, 8asu 80. lrd|ar Ved|c|ra| P|arls. vo|.1. 0e|radur:
lrlerral|ora| 8oo| 0|slr|oulers, 199, 2591-3.
9. Raslog| RP, Ve|rolra 8N. Corperd|ur ol lrd|ar Ved|c|ra| p|arls. vo|.
1. Luc|roW: Cerlra| 0rug Researc| lrsl|lule, 2002, 531.
l|
10. Ko|ale CK. Pracl|ca| P|arracogrosy. 5 ed. NeW 0e|||: va||ao|
Pra|as|ar, 2001, 10Z-13.
l|
11. Evars wC. Trease ard Evars P|arracogrosy. 15 ed. Lordor:
3aurders, 2001, 13Z-1.
rd
12. 0|os| VN. Furdarerla|s ol Exper|rerla| P|arraco|ogy. 2 ed.
Ca|culla: 3c|erl|l|c 8oo| Agercy, 1981,153-Z.
13. Tr|rder P. 0elerr|ral|or ol g|ucose |r o|ood us|rg g|ucose ox|dase W|l|
ar a|leral|ve oxyger acceplor. Arr c||r o|oc|er 199, : 21.
11. 3ac|deWa A, Ra|ra 0, 3r|vaslava AK, K|erar| L0. Ellecl ol /ege|e
marme|ous ard l|o|sous rosas|nens|s |eal exlracl or g|ucose lo|erarce
|r g|ucose |rduced |yperg|ycer|c rals. J Erv|ror 8|o| 2001, 22, 53-Z.
15. Joy KL, Kullar R. Arl|-d|aoel|c acl|v|ly ol P|ororrn|za kurrora exlracl. J
El|arop|arraco| 1999, Z: 113-18.
1. Aqu|ro R, Vore||| 3, Lauro VR, Aodo 3, 3a|ja A , Tora|ro A.. P|ero||c
corsl|luerls ard arl|ox|darl acl|v|ly ol ar exlracl ol /nrnur|um
vers|ou|ar |eaves. J Nal| Prod 2001. 1: 1019-23.
1Z. 0||aWa l, 0s||s| N, Yag| K. Assay lor ||p|d perox|de |r ar|ra| l|ssue oy
l||ooaro|lur|c ac|d reacl|or. Ara| 8|oc|er 19Z9, 95: 351-8.
rd
18. la|||We|| 8, 0uller|dge JVC. Free rad|ca|s |r o|o|ogy ard red|c|re. 2
ed. 0xalord: C|arerdor Press, 1989, 215
3ara ver|ales| er a| Arl|d|aoel|c Acl|v|ly ol Panoanus ooorar|ss|mus Rool Exlracl
lrd J P|arr Edu Res, 0cl-0ec, 2012/ Vo| 4o/ lssue 4 345
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