Results and Discussions Nutrient agar plate (NA) o Unknown specimen (J) on NA shows growth of cream colored colonies

es representing bacterial colonies. Monnitol salt agar plate (MSA) o J on MSA exhibits inhibited growth, suggesting gram negative bacterial colony o Staphylococcus aureus on MSA exhibits growth of yellow colonies; the agar possess a ph indicator changing the media from reddish-pink to yellowish-green suggesting a positive sign for fermentation (Monnitol fermenter). o Micrococcus luteus on MSA exhibits growth of yellow glossy colonies, agar maintain its reddish-pink color suggesting no sign of fermentation (Non-monnitol fermenter). Starch agar plate (SA) o J on SA exhibit growths of cream colored colonies, application of iodine on the media brought out a change on media color from white to black. The iodine reacts to the starch still present on the media staining it black in color; this suggests a negative result toward starch hydrolysis. o Bacillus subtilis on SA exhibits growth of cream colored colonies, application of iodine on the media brought out a change on media color from white to black but regions around the colonies remained the same. The regions around the bacterial colonies are remained unstained since Bacillus subtilis secretes amylase; the starch becomes degraded, thus preventing the change on color brought upon by the iodine. This suggests that Bacillus subtilis is positive for starch hydrolysis. Eosin methylene blue agar plate (EMB) o J on EMB exhibits growth of dark colored colonies with green metallic sheen; this suggests that J is a gram negative bacterium, since EMB is selective toward gram negative bacterial colonies. The results also suggests that J exhibits lactose fermentation and acid production. o Escherichia coli on EMB exhibits growth of dark colored colonies with green metallic sheen this suggests lactose fermentation and acid production. o Klebsiella oxytoca on EMB exhibits growth of dark colored mucoid colonies with green metallic sheen this suggests lactose fermentation and acid production. MacConkey agar plate (MCA) o J on MCA exhibits growth of pink colonies; this suggests that J is a gram negative bacterium, since MCA is selective toward gram negative bacterium. The result also suggests that J exhibits lactose fermentation and acid production. o Escherichia coli exhibits growth of pink colonies. This suggests lactose fermentation and acid production. o Pseudomonas aeruginosa exhibits growth of cream colored colonies. In addition, the MCA changed in color from reddish-pink to yellowish-green, this suggests no fermentation of lactose. Triple sugar iron agar slant (TSIA) o J on TSIA exhibits K/A type of growth that has a red slant and yellow butt, this suggest an alkaline slant and an acid but. Other than that, J exhibits gas production. o Pseudomonas aeruginosa on TSIA exhibits K/K type of growth that has a red slant and butt, this suggest that both slant and butt are alkaline. Pseudomonas aeruginosa does not exhibit gas production. o Klebsiella oxytoca on TSIA exhibits A/A type of growth that has a yellow slant and butt, this suggests that both slant and butt are acidic. Klebsiella oxytoca exhibits gas production. Lysine iron agar slant (LIA) o J exhibits a negative reaction towards LIA, having a purple slant and a yellow butt. o Pseudomonas aeruginosa exhibits a positive reaction towards LIA suggesting lysine decarboxylation, having purple slant and butt. Both slant and butt is alkaline. o Klebsiella oxytoca exhibits a negative reaction towards LIA, having a purple slant and a yellow butt. Simmons citrate agar slant (Citrate) o J on Citrate exhibits growth but the green color of the media remains the same. o Klebsiella oxytoca on Citrate exhibits growth and change of medium color from green to blue; this is because of the use of citrate as the main source of carbon increases the pH of the medium. The increase in pH then causes color change in the bromothymol blue indicator, turning it blue. Oxidative/ fermentation tube deep (OF) o J on OF changed the medium from green to yellow, a clear sign for fermentation and acid production. o Klebsiella oxytoca OF changed the medium from green to yellow, a clear sign for fermentation and acid production. Tryptone broth o J on tryptone exhibits cloudy growth on the broth, and shows positive result towards indole test (utilization of kovacs reagent) producing a cherry red ring on top of the medium. o Klebsiella oxytoca on tryptone exhibits a sedimentary type of growth on the broth, and shows a positive result towards indole test producing a cherry red ring on top of the medium.

Nutrient gelatin deep (NG) o J on NG exhibits growth of cream colored colonies. After subjecting the tube to an ice bath, the media solidify; suggesting that the bacterial colony is negative for enzyme gelatinase. o Staphylococcus aureus on NG exhibits growth of cream colored colonies. After subjecting the tube to an ice bath, the media remained liquid; suggesting that Staphylococcus aureus is positive for enzyme gelatinase. Glucose fermentation broth (GFB) o J on GFB exhibits a change in media color from red to yellow. Like MSA, GFB also has a ph indicator which determines bacterial colonies that ferments and produces acid. J also produces pyruvate bubbles within the Durham tube. o Escherichia coli on GFB exhibits a change in media color from red to yellow, suggesting glucose fermentation and acid production. o Klebsiella oxytoca on GFB exhibits a change in media color from red to yellow, suggesting glucose fermentation and acid production. Pyruate gas is also collected within the Durham tube. Urea agar slant (Urea) o J on Urea exhibits growth of cream colored colonies and maintains the yellow coloration of the media, suggesting a negative urease test. o Klebsiella oxytoca on Urea exhibits the change in medium color from yellow to pink, suggesting a positive urease test. Nitrate broth o J on Nitrate exhibits flocculent colonial growth. After applying nitrate reduction test the broth is stained red-brown, suggesting presence of nitrate reductase. o Staphylococcus aureus exhibits sediment type of colonial growth. After applying nitrate reduction test the broth maintains its yellow color, suggesting Staphylococcus aureus is negative for nitrate reductase. Motility indole ornithine agar deep(MIO) o J on MIO agar change medium color from blue to yellow suggesting the colony is positive for motility, indole production and decarboxylase activity. o Escherichia coli on MIO agar change medium color from blue to yellow suggesting the colony is positive for motility, indole production and ornithine decarboxylase activity. o Micrococcus luteus on MIO agar medium color from purple to red suggesting the colony is negative for motility, positive for ornithine decarboxylase activity, and indole production. Nutrient broth (NB) o J on NB exhibits a sediment type of colony growth. Broth changes color to red in methyl red test, suggesting the bacterial colony produces stable acids. o Escherichia coli on NB exhibits a sediment type of colony growth. Broth changes color to red in methyl red test, suggesting the Escherichia coli produces stable acids. o Pseudomonas aeruginosa on NB exhibits a cloudy colony growth. Broth color remains the same after letting it set, suggesting a negative methyl red test result. o Bacillus subtilis on NB exhibits a cloudy colony growth. Broth color remains the same after letting it set, suggesting a negative methyl red test result. Nutrient broth with 0.5% glucose (NB 0.5%) o J on NB 0.5% exhibits no initial color change and media color change after methyl red test, suggesting the bacterial colony is positive in producing stable acids. o Pseudomonas aeruginosa on NB 0.5% exhibits no initial color change and no media color change after methyl red test, suggesting Pseudomonas aeruginosa is negative for acid production. o Klebsiella oxytoca on NB 0.5% exhibits an initial color change to pink and no media color change after methyl red test, suggesting Klebsiella oxytoca is negative for acid production.