Programme: I- M.Sc., (Biotechnology) Course : Animal Biotechnology Part A (14X1=14) Answer ALL the questions. 1.

. The tissue culture is the technique in which tissue one grown in a)Suitable medium b) BSS c) agar medium d) agarose medium 2. Primary culture is suitable for.. a)vaccine production b) pharmaceutical purpose c)vitaminproduction production

TRUE or FALSE: 11. In mammols,tolipotert cells have potenaleol to become adelt body 12. Growth hormone is synthesized in the gonadotroptic cells 13. Hematopoielic stem cells develop RBC,WBC and platelets 14. Bone marrow trens placlition programme stated in 1986 Part B (4X3=12) Answer any FOUR questions. 15. Explain the types of disaggregation of tissue

d)enzyme 16. Explain the cell separation and maintance of cell culture 17. Give an account on scaling up of animal cell culture 18. Explain the ESC cells and their application 19. Explain apoptosis 20. What are the applications of animal cell culture Part C (3X8=24) Answer any THREE questions. 21. Give an detail account on cell synchronization 22. Explain cell transformation 23. Write the type of stem cells and its applications in human tharaphy 24. Explain the method of seri culture and its applications in human therapeutic proteins 25. What is trangenic animal with goat,sheep and fishes as an example.

3. Established cell lines behave in similer manner with a) Irrespective of their origin b) they have similer nutritional requirenment c) they grow much higher densities than primary cell lines d) d)all the above 4. Primary cell lines retain their a) Diploid karyotype b)triploid karyotype b) polyploidy d)none of the above Fill in the blanks: 5. Blood plasma is the ------------ in which cells could survine 6. Once a primary culcture is subcultured it becomes as --------7. The used ratio of medium volume to surface area is---------8. The cell synchronization method ----------9. The duration of mphase can be calculated by ----------10. Several agents that prevent DNA replication have been used to prepare -----

Course (14X1=14)

: Basic Industrial Biotechnology Part A

15. Define antibiotic and their types 16. Importance of Penicillin. 17. Role of vitamins in pharmacy 18. Define cellulose. 19. Application of biofertilizers.

Answer ALL the questions. A) Choose the best answer

1. Father of antibiotics. (i) Flamming (ii)Janner (iii) Hook (iv) Watson Part C (3X8=24) 2. Penicilin was described by -------------------Answer any THREE questions. ----(i) 1929 (ii) 1932 (iii)1875 (iv) 1930 3. Vitamin B12 was isolated by. 20. Production process of penicillin and their (i) E.L Ricke (ii) Watson & Crick (iii) application. Goldsby (iv) Robert 4. Enzymes are ---------------------21. Briefly describe the steroids (I)Bio catalyst (ii)Protein receptor (iii) 22. Production of biopesticides and Solvent (iv) Substrate biofertilizers. 5. SCP was inverted by. (i) 1932 (ii) 1966 (iii) 1969 (iv) 1945 23. Describe single cell protein. B) True or false 6. Antibiotic used as animal growth promoters and in veterinary medicine. 7. Riboflavin is an artificial chemical vitamins. 8. Microbial rennin is next in order of significant enzymes. 9. Mushroom is a muticellular protein

D) Match the following 10. Single cell protein 11. Lipase enzymes 12. Macrolides binds 13. Penicillin chain 14. - amylases

- - Lactase Industrial - 1,4 glycosidie Human food

- Erythromycin

Part B Answer any FOUR questions.

(4X3=12)