PROJECT SEMESTER REPORT B.

Tech Biotechnology (January-June, 2011)

ISOLATION AN S!"##NIN$ O% IN&LINAS# '"O &!IN$ %&N$I %"O( ")I*OS')#"# SOIL O% IN&LIN !ONTAININ$ 'LANTS

Su+,itte- +y Danish goyal Roll No. 700900036 &n-er the $ui-ance o. Dr.Sumit ain

Department of Biotechnology & Environmental Sciences

Tha!ar "ni#$rsity% Patiala Jun$% &0''

T()*E O+ CONTENTS
S.NO !)A'T#"S ABST"A!T 1. 2. 2.1 2.1.1 2.1.2 2.1.5 2.2 2.2.1 2.2.2 2.2.5 INT"O &!TION "#/I#0 O% LIT"#AT&"# Inulina2e-e34re22ing ,icroorgani2,2 (oul-2 7ea2t Bacteria !haracteri2tic2 o. inulina2e2 (olecular 0eight o. inulina2e2 O4ti,u, 4) an- te,4erature acti:ity o. inulina2e2 #..ect o. ,etal2 ion2 an- 4rotein inhi+itor2 on inulina2e acti:ity 2.5 2.5.1 2.5.2 2.5.5 2.5.; 5. 5.1 5.2 5.5 5.; A44lication2 o. inulina2e2 )igh .ructo2e 2yru4 Inulo-oligo2acchari-e 4ro-uction Bioethanol 4ro-uction Other a44lication2 o. inulina2e2 (AT#"IALS AN (#T)O S (aterial2 u2e!ollection2 o. 2a,4le2 I2olation o. inulina2e 4ro-ucing .ungi 'uri.ication an- ,aintaince o. .ungal i2olate2 21 21 22 22 25 2; 26 26 26 26 'A$# NO. 1 2 1 15 16 16 18 19 19 20 21

5.6 5.1 5.1.1 5.1.2 5.1.2.1 5.1.2.2 5.1.2.5 5.1.2.; 5.8

!haracteri<ation an- I-enti.ication o. .ungal i2olate2 Screening an- en<y,atic e2ti,ation #n<y,e 4ro-uction ,e-ia u2e#n<y,atic a22ay "eagent2 re=uireSu+2trate2 Sto44ing reagent ( NS reagent) Stan-ar- 2toc> 2olution #..ect o. te,4erature, 4) an- ,etal ion2 on inulina2e acti:ity +y 4otential 4ro-ucer A.niger $1

21 21 21 21 28 28 28 29 29

5.8.1 5.8.2 ;. ;.1 ;.2

#..ect o. 4) an- te,4erature #..ect o. ,etal ion2 an- -etergent2 "#S&LTS AN ISS!&SSIONS

29 2? 50 51 58

I2olation an- i-enti.ication o. .ungi Screening an- e2ti,ation o. .ungal i2olate2 .or inulina2e acti:ity

;.5

#..ect o. te,4erature, ') an- ,etal ion2 on inulina2e acti:ity +y 4otential 4ro-ucer A.niger $1

5?

;.5.1 ;.5.2 ;.5.5 6. 1.

#..ect o. te,4erature #..ect o. 4) #..ect o. ,etal ion2 an- -etergent2 S&((A"7 AN !ON!L&SIONS "#%#"#N!#S A''#N I@

5? ;0 ;1 ;2 ;; 66

TABL#S S.NO 2.1 2.2 2.5 2.; ;.1 ;.2 TABL#S Su+2trate2 inulina2e 4ro-uction u2e- .or 1 Inulin content2 o. 2o,e 4lant2 9 Inulina2e e34re22ing ,icroorgani2,2 'ro4ertie2 o. 2o,e ,icro+ial inulina2e 'A$# NO. ? 11 15 1?

i2tri+ution o. 2a,4le2 in -i..erent ha+itat2 51 %ungal i2olate2 Aith their ,or4hological 55 characteri2tic2

;.5 ;.;

%ungi i2olate- .ro, rhi<o24heric 2oil Inulina2e acti:ity o. culture .iltrate2

5; 59

S. NO 2.1 2.2 Structure o. inulin

LIST O% %I$&"#S

'A$# NO 8 12

Inulin +eing acte- u4on +y ,icro+ial e3o- an- en-o-inulina2e en<y,e2.

;.1 ;.2 ;.5 ;.;

!ulture characteri2tic2 o. 2electe- .ungi on S A ,e-iu, #..ect o. te,4erature on inulina2e acti:ity 5; #..ect o. 4) on inulina2e acti:ity 56 #..ect o. ,etal ion2 on inulina2e acti:ity 51

56 5? ;0 ;1