Water Potential/Osmotic & Pressure Name: ................................................ Student ID: .........................

WS - 02

1.

Which beaker(s) contain(s) a solution that is hypertonic to the bag?

a. Beaker 3 b. Beakers 2 and 4 d. Beaker 4 e. Beakers 3 and 4 2.

c. Beakers 1, 2, and 5

Which bag would you predict to show the least change in mass at the end of the experiment?

a. The bag in Beaker 1 b. The bag in Beaker 2 d. The bag in Beaker 4 e. The bag in Beaker 5

c. The bag in Beaker 3

3. Arrange the beakers in order of the mass of the bags inside them after the experiment has run for 30 minutes. List the bag that loses the most mass first.

a. 1,2,3,4,5

b. 1,5,2,3,4

c. 4,3,2,5,1

d. 3,2,1,4,5

e. 2,1,5,3,4

4. In beaker B, what is the water potential of the distilled water in the beaker, and of the beet core?

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a. Water potential in the beaker = 0, water potential in the beet core = 0 b. Water potential in the beaker = 0, water potential in the beet core = -0.2 c. Water potential in the beaker = 0, water potential in the beet core = 0.2 d. Water potential in the beaker cannot be calculated, water potential in the beet core = 0.2 e. Water potential in the beaker cannot be calculated, water potential in the beet core = -0.2 5. Which of the following statements is true for the diagrams?

a. b. c. d. e.

The beet core in beaker A is at equilibrium with the surrounding water. The beet core in beaker B will lose water to the surrounding environment. The beet core in beaker B would be more turgid than the beet core in beaker A. The beet core in beaker A is likely to gain so much water that its cells will rupture. The cells in beet core B are likely to undergo plasmolysis.

6. Match each Term on the left with the best Descriptor on the right. Each Descriptor may be used only once Term Concentration Diffusion A B Descriptor Moves oxygen into cells

Equal amount of water inside a cell as outside

Allows some substances through

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More water outside a cell than inside

Selectively permeable membrane Osmosis

E F

Amount of a substance in a certain place

More water inside a cell than outside

Moves water into and out of cells

Essay

1. There are two components to water potential: solute concentration and pressure. How do you think this fact affects the movement of water into and out of cells? For example, can two solutions that differ in their solute concentration be at equilibrium in terms of water movement? Can a solution with a molarity of 0.2 be in equilibrium with a solution with a molarity of 0.4?

2. The molar concentration of a sugar solution in an open beaker has been determined to be 0.3M. Calculate the solute potential at 27 degrees. Round your answer to the nearest hundredth. 3. The pressure potential of a solution open to the air is zero. Since you know the solute potential of the solution, you can now calculate the water potential. What is the water potential for this example? Round your answer to the nearest hundredth. 4. Can you give a plant too much fertilizer? Explain why or why not. . 5. Given the following scenarios draw an arrow representing the movement of water across these semi-permeable membranes. a. b. c.

= 1.2

= 3.2

=0

= 2.3

= -2.3

=0

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6. For the following molarities of sucrose, calculate the water potential of each. S = -I C R T. Sucroses ionization constant is 1. The pressure constant (R) is 0.0831 liter bars/mole K. And the experiments was run at 23 C. (Convert to Kelvin by adding Celsius to 273). a. 1.1 Mol/Liter = __________________ b. 0.3 Mol/liter = ___________________ c. 0.0 Mol/Liter = ___________________ d. 7 Mol/Liter = ___________________ 7. If You trying to repeat these experiments at the North Pole. The temperature is -3 C, calculate the new for each problem. e. = ___________________ f. = ___________________ g. = ___________________ h. = ___________________ 8. Describe the relationship of temperature to water potential. 9. Describe the relationship of tonicity to water potential.

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