Professional Documents
Culture Documents
Venipuncture
Course &
Training Kit
PREFACE
A basic understanding of general human (or veterinary)
anatomy and physiology, especially the cardiovascular
system, is required in order to understand and safely apply
the techniques that the student will learn.
Every simple venipuncture procedure is in essence a
minor surgical procedure governed by the basic principles
of surgery:
Have a comprehensive understanding of basic
medical sciences, especially anatomy and physiology
of the relevant areas and systems.
Follow the basic principles of sterility and asepsis,
including the use of barrier techniques.
Take a medical history and interpret this information
to modify your treatment plan.
Use good lighting.
Respect life and bodily tissues.
Perform the procedure in a humane and professional
manner.
Anticipate the possibility of complications and deal
them promptly and effectively.
The Apprentice Doctor Venipuncture Skills Course
and Kit are not intended to be used as a substitute for
clinical training. Instead, The Apprentice Doctor offers
a firm foundation so students can successfully master
the initial learning curve in a non-clinical setting before
confidently entering the clinical situation.
The Apprentice Doctor Venipuncture Course and Kit
consist of:
1. The Apprentice Doctor Venipuncture Course on
DVD-ROM
2. The Apprentice Doctor Venipuncture Kit with a
Venipuncture Trainer, as well as a variety of medical
items to complete all the practical projects.
IMPORTANT NOTES:
The information offered in The Apprentice Doctor
Venipuncture Course material is based on recent
guidelines set by the World Health Organization (WHO),
and the Centers for Disease Control and Prevention (CDC).
The References section gives more information with links
to help you keep track of the latest information.
Keep in mind that specifics may differ from the information
or protocol of your local hospital or training institution.
In a clinical field, there are often a number of acceptable
protocols, knowledge of more than one offers students
a fuller picture. Protocols and standards may vary in
different regions and countries, as well as in different
FOREWORD
In any practical endeavorfrom writing a book, to
painting a picture, to performing a surgical operationa
solid foundation in the basic skills of the discipline is an
essential prerequisite.
The days when clinical skills were simply taught from senior
to junior, from one year to the next are long gone. Clinical
skills require an in-depth knowledge of the procedure
as well as the opportunity to practice in a simulation
environment, with definite guidelines to follow, and
parameters to evaluate the students progress. Students
need to train in a non-clinical setting until they have the
WARNINGS
Before starting the course, please read these warnings
carefully:
The Apprentice Doctor Venipuncture Course and
Kit is an Educational product. All items in this kit are
intended exclusively for non-clinical purposes. Do not
use any of these items on actual human or animal
patients, even in an emergency.
This kit and its components are intended exclusively
for training/educational purposes. They are not
intended for use in any clinical setting, or in the cure,
mitigation, treatment, or prevention of disease in man
or other animals.
The Apprentice Doctor Venipuncture Course and
Kit contains sharp instruments. Please be cautious!
Keep out of reach of children of 14 years and younger,
especially toddlers and babies.
Adult supervision is required for students 15-17 years
of age. It is essential that students 18 years and older
take extreme care while doing the practical projects.
Some items may contain latex rubber, not suitable for
persons with latex allergies.
Always use clean items. Wash used items with liquid
soap and water after each session then leave in an
antiseptic solution (e.g., Savlon) for 60 minutes. Rinse
thoroughly with clean water and dry before replacing
in the kit.
VIDEO
Sharps will be used!
Sharp or potentially sharp medical items or objects (e.g., glass medicine vials) will be used.
Perform the procedures in these projects with great caution and care.
Discard sharp and potentially sharp items ONLY in the sharps waste container.
Study the section ASPECTS OF SAFETY before doing these projects.
Blood hazard
SIMULATION PROCEDURE
Procedure may only be performed on real patients in a proper medical setting by either
qualified medical professionals or students under proper supervision in a formal training
facility with all relevant legalities and medical indemnities in place.
These procedures can be practiced on a suitable fellow student or other adult volunteer.
DISCLAIMER
The producer or supplier of this application does not:
Offer any warranty regarding the accuracy or
correctness of any information contained in this
application.
Assume any responsibility for any damage or
consequential damage related in any way to the
information, instrumentation, or items contained in
this product/application or as a result of their use.
The user takes full and exclusive responsibility for the safe
application of any information contained in this application.
The user also takes full and exclusive responsibility for
all safety aspects related in any way to the use of any
instrument, or item supplied with this application. This
exclusive responsibility applies equally to the user or to
any person being supervised by the user.
No warranties are offered on the functional status or fitness
for the specific application of any information, instrument
or item supplied in this application.
The supplier accepts no responsibility for the malfunction
of any instrument or item. (The buyer will be entitled to
the replacement of such defective items within the time
limits of the Basic Terms and Conditions).
REIMBURSEMENT POLICY
The Apprentice Corporation is confident that you will
be satisfied with this product in each and every way, as
supported by our extremely low return statistics.
If for any reason, you are dissatisfied with your choice,
The Apprentice Corporation will be happy to reimburse
you (less postage and shipping charges) should you
wish to return the complete medical kit, as well as the
DVD-ROM in an undamaged state within 8 weeks of
purchase.
Please be ethical. It is simply unfair to order and open
the kit, as well as some of the items then copy the course
material on your computer or other electronic device
and then expect a refund on returning the product.
Before returning, delete ALL copies of the course
RECOMMENDATIONS ON
HOW TO USE THE APPRENTICE DOCTOR VENIPUNCTURE KIT
VIDEO
To gain maximum benefit from The Apprentice
Doctor Venipuncture Course and Kit, the following
guidelines should be followed:
Read the WARNINGS and DISCLAIMER sections
attentively. They are available on The Apprentice
Doctor Venipuncture DVD.
Work systematically through course material. Be
sure that you understand each section and can
perform the practical projects skillfully before
proceeding to the next section. Do not skip a
section because you think it is unimportant or too
simple. Although basic principles often appear to
be simple, you must understand and practice these
simple building blocks in order to succeed later
with more complicated applications. Perform the
practical skills projects only when you understand
the theory involved in that specific section.
Start with the Introduction section. You will learn
about the items contained in The Apprentice
Doctor Venipuncture Kit. Section 2 contains
vital information about taking a medical history,
sterility and asepsis and offers the opportunity to
practice relevant basic skills.
Systematically study The Apprentice Doctor
Venipuncture Course Sections 3, 4 and 5 in
order and perform all of the practical projects using
your Venipuncture Trainer where applicable.
Do not omit Section 5 on complications it is
of utmost importance that one has a thorough
10
INDEX
PREFACE
FOREWORD
WARNINGS
KEYS TO SYMBOLS
DISCLAIMER
REIMBURSEMENT POLICY
10
INDEX
11
SECTION 1: INTRODUCTION
15
16
18
19
22
24
25
28
28
29
30
31
32
33
34
35
36
Blood
36
Blood plasma
36
Whole blood
37
Blood cells
37
37
Hemoglobin
37
Hematology
37
SECTION 2 : PREPARATION
39
Case study 2: Contracting One of the Most Feared Diseases in the World Today
40
41
Patient information
42
42
43
PROJECTS 3A 3I
43
43
46
46
47
11
47
47
47
48
48
PATIENT POSITIONING
49
TOURNIQUETS
51
51
53
54
54
55
57
59
61
63
69
PROJECT 6C HOW TO PREPARE THE PUNCTURE SITE ASEPTICALLY (COLLECTING BLOOD FOR BLOOD CULTURE)
71
PROJECT 6D HOW TO PREPARE THE PUNCTURE SITE ASEPTICALLY (COLLECTING BLOOD FROM BLOOD DONOR)
73
75
76
77
77
79
81
Intradermal injections
84
85
88
89
90
90
90
90
90
91
96
99
102
106
107
107
107
VETERINARY VENIPUNCTURE
108
108
109
VeinViewer
109
AccuVein
109
Breastlight
109
Ultrasound
109
12
Radiography
109
110
112
114
BLOOD TRANSFUSIONS
117
117
Agglutination
118
Blood donations
119
120
123
Case study 4: Despite All the Training and the Necessary Care, Accidents Do Happen
124
125
ARTERIAL CATHETERIZATION
126
CORONARY ARTERIOGRAPHY
127
INTERVENTIONAL RADIOLOGY
127
KIDNEY DIALYSIS
128
KIDNEY DIALYSIS
129
PORTS
129
130
ANESTHESIA
130
Topical Anesthetic
130
Local Anesthesia
130
130
131
134
Regional Anesthesia
134
General Anesthesia
135
Infusion Pumps
135
NEW DEVELOPMENTS
135
135
Needleless Injections
135
136
Saliva
136
Sputum
136
Breast milk
136
Semen
136
Sweat
137
137
Urine
137
Urinalysis
137
Feces
137
138
138
Ascites
138
Effusion
138
Pleural
138
Joint effusion
139
139
Pus
139
13
SECTION 5: COMPLICATIONS
141
142
143
Allergic responses
144
Contact dermatitis
144
Skin rash/Urticaria
144
144
145
Hematoma
145
Ecchymosis
146
146
146
147
Catheter-related infections
147
148
148
149
Superficial phlebitis
149
Septic thrombus
150
150
Embolism
151
Air embolism
151
153
Nerve damage
153
Arterial cannulation
153
153
153
SECTION 6: CONCLUSION
155
ASSESSMENT MODULE
156
EPILOGUE
156
REFERENCES
156
CREDITS
157
GLOSSARY
160
14
CASE STUDY 1:
AN AVOIDABLE ACCIDENTAN UNNECESSARY DEATH
16
The Venipuncture Trainer in this kit has been designed with effectiveness and affordability in mind. Students of phlebotomy
will be able to use this versatile Venipuncture Trainer to practice basic skills over and over while experiencing a realistic feel
similar to the real clinical situation. As an optional extra, The Apprentice Corporation has artificial arms available for sale.
Keep in mind that no training system can replace the clinical phase of your training. Practice, gain confidence, then face
real clinical situations under supervision and always learn from both your successes and your failures.
PHLEBOTOMY
Phlebotomy is the procedure of removing (drawing)
blood from the vascular system by puncturing a vein
or sometimes an artery with a needle or by making an
incision (rarely) to obtain a blood sample for:
Diagnostic purposes
To be analyzed by a medical laboratory
Therapeutic purposes
-- To treat polycythemia vera, a condition that causes
an elevated red blood cell volume (hematocrit).
Phlebotomy is also prescribed for hepatitis B and
C and for patients with disorders that increase the
amount of iron in their blood to dangerous levels,
such as hemochromatosis. Phlebotomy may be
performed on patients with pulmonary edema to
decrease their total blood volume.
-- Collecting blood from blood donors, commonly
one unit of blood (500 mL) in a session.
17
TYPE
Isotonic
Isotonic
SOLUTION
(EXAMPLE)
USES
SPECIAL
CONSIDERATIONS
Dextrose 5% in water
Fluid loss
(D5W)
Dehydration
patients
Hypernatremia
Shock
Hyponatremia
Blood transfusions
Resuscitation
Fluid challenges
DKA (diabetic ketoacidosis)
Isotonic
Ringers Lactate/Lactated
Dehydration
Ringers (LR)
Burns
Hypotonic
Water replacement
normal saline)
DKA
Hypertonic
Dextrose 5% in
normal saline
Hypertonic
Temporary treatment
18
PROJECT 1A
FAMILIARIZE YOURSELF WITH YOUR VENIPUNCTURE KIT
Use the Content List and follow these steps to ensure that your Apprentice Doctor Venipuncture Kit and Trainer is
complete. Learn the names and functions of each item as you go.
VIDEO
WARNING
This kit contains sharp items that can be potentially
hazardous if they are not used correctly and safely. Keep
the kit and contents away from babies and children under
the age of 15. Adult supervision is required for students 1517. It is essential that all students take extreme care while
doing the practical projects.
Prepare yourself for the clinical situation and imagine
19
REQUIREMENTS
Venipuncture Kit and Trainer and a clean, uncluttered work surface. Follow these steps:
STEP 1
20
IV Catheter
Venipuncture Trainer
Connectors /
Lumen stoppers
Disposable Tourniquet
Butterfly Needles
Regular Syringes
IV Fluid Bag
Regular Needles
IV Lines
Safety Needles
Glass Vial
Plastic Vial
10
Safety Vacuum
Container Device
Gauze Squares
10
pairs
Gloves
pairs
pairs
Cotton Wool
Roll of Strapping
Reusable Tourniquet
* PLEASE NOTE:
Tournistrip
Transparent Dressing
21
STEP 2
Open the Venipuncture Trainer and compare it with the
illustration below.
STEP 4
Replace all the items in your Kit and proceed with the
Venipuncture Course, or close the kit and place it in a safe
location out of the reach of children.
POINTS OF INTEREST
The trainer is simple, effective, and functional.
STEP 3
Buy red food colorant available from grocery stores and
add to the kit.
22
IMPORTANT!
If you are injured or pricked by a needle or other sharp
object or get blood or other potentially infectious
materials in your eyes, nose, mouth or on broken skin,
immediately flood the exposed area with water and clean
any wound with soap and water or a skin disinfectant if
available. Immediately report this to your employer and
seek immediate medical attention.
Credit: U.S. Department of Labor
23
Alternatively contact us per email and we will mail a selfaddressed box. Return with your sharps container for safe
disposal.
Email to enquiries@theapprenticedoctor.com
24
PROJECT 1B
HOW TO USE A SAFETY NEEDLE/DEVICE
It is all about safetyfor your patients AND YOU!
VIDEO
WARNINGS:
INFORMATION
25
FOLLOW THESE
INSTRUCTIONS EXACTLY:
STEP 1
Look at the diagram of the needle with its various parts
STEP 7
Withdraw the plunger of the syringe to fill the syringe with
3-5 ml of air, just for practicing purposes.
STEP 8
Pull the safety mechanism back and hold gently, just on
the one side, in the activated position with your middle or
index finger.
STEP 9
Remove the protective sleeve of the needle.
STEP 10
STEP 2
STEP 11
STEP 3
Wash your hands. Start now to develop this simple
but effective habit. If you like, don clean gloves (gloves
optional).
STEP 4
Orientation is important when opening the AutosafeReflex safety needle. Hold the needle with the paper
cover facing up. The needle is packed with its bevel facing
up, towards the paper cover. Kept in this orientation, the
needle will be positioned correctly for performing clinical
procedures like venipuncture.
STEP 5
Lift the edge of the paper cover and peel it backwards.
Pinch the package at the fold (at the base of the package)
and fold the base down.
STEP 6
Fit the needle to the syringe, and withdraw the safety
needle from the package while maintaining the needles
orientation.
26
STEP 12
Remove your index finger from the safety mechanism.
STEP 13
Withdraw the needle; you will notice the reflex mechanism
activates spontaneously and passively. The sharp needle
tip will be covered by the safety cap, in a somewhat offcenter position.
STEP 14
Remove the needle by disconnecting it from the hub
of the syringe. Discard the used needle into the sharps
waste container. Never try to recap a regular needle or
reassemble a safety needle. In a clinical setting, you will
discard the complete unit (syringe and needle).
NOTE:
In rare instances, after activating the AutosafeReflex needles safety device, you may need to
expose the needle again for example when
withdrawing medication from a vial using the safety
needle. See this demonstration on how to safely
expose the needle again: [VIDEO-CLIP]
Look at the Autosafe-Reflex Vacutainer
Phlebotomy Device it is equipped with an
Autosafe-Reflex needle. Do not open the device at
this stage this device will be used in PROJECT 11 A
DRAW VENOUS BLOOD USING A VACUUM TUBE.
POINTS OF INTEREST
27
[DOWNLOAD PDF]
28
[DOWNLOAD PDF]
29
[DOWNLOAD PDF]
30
[DOWNLOAD PDF]
31
[DOWNLOAD PDF]
32
[DOWNLOAD PDF]
33
[DOWNLOAD PDF]
34
[DOWNLOAD PDF]
35
BLOOD
Blood is classified as a specialized connective tissue from
an embryological point of view. Blood is the fluid that
circulates through the heart, arteries, capillaries, and veins
and is the chief means of transport within the body. It
transports oxygen from the lungs to the body tissues, and
carbon dioxide from the tissues to the lungs. It transports
nutritive substances and metabolites to the tissues and
removes waste products to the kidneys and other organs
of excretion. It has an essential role in maintaining fluid
Blood can be separated into 3 layers by a process of centrifugation (fast spinning of blood filled tubes in a laboratory
apparatus called a centrifuge).
1. The upper yellowish layer is blood plasma.
2. The thin, middle, buffy layer is white blood cells, as well as blood platelets.
3. The bottom layer is packed erythrocytes, or red blood cells. Blood serum is blood plasma without fibrinogen
or the other clotting factors.
BLOOD PLASMA
The liquid phase of the blood, obtained by sedimentation
or centrifugation of blood treated with an anticoagulant
(anti-clotting agent).
Plasma is mostly fluid, consisting mainly of water, as well
as three specific types of proteins (albumin, globulins,
36
BLOOD CELLS
If a drop of human blood is thinly smeared across a
microscopy slide, you can see various different blood cells.
The pink roundish structures with white centers are the
erythrocytes (red blood cells).
HEMOGLOBIN
Hemoglobin has four protein chains, and each protein chain is called a
globin. Hemoglobin consists of four contorted protein globin chains, two
Apha and two Beta.
Packed red blood cells are red blood cells that have
been separated from whole blood for transfusions. Packed
red blood cells (RBCs) essentially contain the same amount
of hemoglobin as whole blood, but most of the plasma
has been removed.
37
CASE STUDY 2:
CONTRACTING ONE OF THE MOST FEARED DISEASES IN THE WORLD TODAY
40
WARNING!
41
PROJECT 2
TAKE A MEDICAL HISTORY
Gather the relevant information and prevent avoidable mishaps!
See The Apprentice Doctor Foundation Course for information on how to take a comprehensive medical history[CLICK
HERE]
PATIENT INFORMATION
When a physician orders a laboratory blood test, a lab requisition form needs to be filled out accurately and signed by
the physician. It is important to have a double-check system on requisition forms and sample labeling to ensure that the
correct blood samples are taken from the correct patient and that the correct results are allocated to the correct patient.
[PRINT A FORM]
A number of forms are available in the kit.
42
SHORT NOTES
ON ASEPTIC TECHNIQUE
Study this section carefully as it can make the difference between frequent and occasional complications and
possibly the difference between life and death!
Follow an acceptable hands-hygiene protocol:
Handwashing techniques
Wash your hands with an acceptable
method before and after every
venipuncture procedure.
Alcohol rub
Hygienically preparing your hands
with an alcohol-based hand sanitizer
before and after procedures is
a permissible way to prepare
uncontaminated hands aseptically.
Contamination
may
be
any
environmental dirt, bodily fluids
(e.g., blood) secretions (e.g., saliva) or
excretions (e.g., feces.)
Don clean gloves
PROJECTS 3A 3I
PROJECT 3A
A TECHNIQUE FOR PROPER HANDWASHING
Learn to wash your hands prepare them hygienically before examining a patient
VIDEO
43
INFORMATION
SETTING:
The bathroom or any room with a suitable faucet and sink
for washing hands.
REQUIREMENTS
NOTE:
1. Handwashing can be subdivided into the following
seven important steps.
Open faucet
Wet
Soap
Wash
Rinse
Dry
Close faucet
2. Hands should be washed for at least 40-60 seconds
to be effective.
3. A healthcare workers nails should ALWAYS be kept
neat, short, and hygienically clean!
PROCEDURE:
STEP 1
Turn on the faucet and adjust to a moderate stream of
water. Wet both hands up to the wrists.
44
STEP 2
Apply enough soap to the hands until you have a rich
foamy lather. Completely lather the surface of both hands
and up the wrist.
STEP 3
Repeat the following actions at least five times:
3.1 Rub hands palm to palm.
3.2 Right palm over the back of the left hand with
fingers interlaced and vice versa.
3.3 Palm to palm with fingers interlaced.
3.4 Backs of fingers to opposing palms with
fingers interlocked.
3.5 Rotational rubbing of left thumb clasped in
right palm and vice versa.
3.6 Rotational rubbing, backwards and forwards
with clasped fingers of right hand and vice
versa.
3.7 Rotational rubbing of wrist by opposing palm
and vice versa.
STEP 4
Rinse the hands well. Allow running water to flow over
the hands. If possible let the water run from the fingertips
to the palms and then towards the wrists. Rinse soap off
completely.
STEP 5
Dry hands thoroughly with a single-use disposable paper
towel. Start at the fingers, work to the palms and back of
the hands, and lastly dry the wrist areas. Use the same
towel to turn off the faucet. Alternatively use your elbow
to close the faucet. Do not use your clean hands.
STEP 6
Your hands are now hygienically prepared. If you intend to
perform a clinical examination, don clean gloves.
(See PROJECT 3C)
HINTS:
POINTS OF INTEREST
45
PROJECT 3B
CLEANING HANDS WITH AN ANTISEPTIC RUB
Print out the World Health Organizations (WHO) guideline diagram and follow the steps
VIDEO
PROJECT 3C
HOW TO DON (PUT ON) CLEAN GLOVES
Print out the World Health Organizations (WHO) guideline diagram and follow the steps
46
Touching only the cuff, take the first glove out of the
original box.
Try to touch only the wrist area of the glove, i.e., the
top end of the cuff.
Don the first glove by sliding it over the fingers, palm,
and wrist.
With the bare hand, take a second glove from the box
again, only touching the gloves cuff or wrist.
PROJECT 3D
HOW TO SAFELY REMOVE USED GLOVES
Print out the World Health Organizations (WHO) guideline diagram and follow the steps
PROJECT 3E *
HOW TO CHANGE INTO THEATER ATTIRE
*See The Apprentice Doctor Foundation Course for further information on how to perform these procedures. [CLICK HERE]
PROJECT 3F *
HOW TO SCRUB FOR A STERILE PROCEDURE
*See The Apprentice Doctor Foundation Course for further information on how to perform these procedures. [CLICK HERE]
PROJECT 3G *
HOW TO GOWN FOR A STERILE PROCEDURE
*See The Apprentice Doctor Foundation Course for further information on how to perform these procedures. [CLICK HERE]
47
PROJECT 3H
HOW TO DON STERILE GLOVES
Print out the World Health Organizations (WHO) guideline diagram and follow the steps
PROJECT 3I*
HOW TO REMOVE CONTAMINATED GLOVES
*See The Apprentice Doctor Foundation Course for further information on how to perform these procedures. [CLICK HERE]
48
PATIENT
POSITIONING
PERFORMING VENIPUNCTUREON A VEIN OF THE UPPER
EXTREMITY
Position the patient comfortably with their arm at heart
level or just below. Outpatients should be placed in the
sitting position and hospital patients lying in bed, in the
semi-Fowlers or supine position.
Inspection and palpation are essential components
for selecting a suitable vein; therefore the intended
venipuncture site must be exposed. Good lighting is
required. If needed, position the light at an angle to
enhance inspection of the veins.
Place a clean linen-saver below the arm to protect bed
sheets.
Ensure that all the venipuncture equipment and items that
you need are within easy reach.
49
50
TOURNIQUETS - PROJECTS 4A 4D
PROJECT 4A
HOW TO APPLY A TOURNIQUET (DISPOSABLE)
A simple technique to facilitate easier venipuncture
INFORMATION
A tourniquet is a constricting or compressing device used
to control (stop or reduce) venous or arterial circulation to
an extremity for a period of time.
IMPORTANT NOTE
REGARDING ARTERIAL
TOURNIQUETS:
A surgeon may use an arterial tourniquet under controlled
conditions within specific time limits to stop the arterial
blood flow to a limb. However, in the following discussion
we will exclusively focus on the use of tourniquets to reduce
or stop the venous return of blood to the heart for a period
of time.
A venous tourniquet is usually applied 7-10 cm (3-4 inches)
above the intended venipuncture point on either the upper
or lower extremity. The idea behind applying a tourniquet
51
REQUIREMENTS
You will need:
A volunteer test patient
One disposable (latex-free) tourniquet
A re-usable tourniquet
A blood pressure cuff (if you have one available)
STEP 2
Remove clothing from the arm up to the middle of the
upper arm. Place the tourniquet about 7-10 cm (3-4
inches) above the elbow.
STEP 3
Place the tourniquet under the patients arm with an end
in each hand. Ensure that it lies flat on the skin surface
STEP 4
Swap the two tourniquet ends to opposite hands so
that the end on the right is closer to you. Pull the ends
upwards to form an X.
52
STEP 5
Fold the end on the right side over on itself. Pull both
ends upwards, with the end on the right side being
somewhat tighter.
STEP 6
Tuck the double-folded end halfway under the other left
end, leaving the free end, approximately 5 cm (2 inches)
long, pointing away from you. Ask your volunteer patient
to clench a fist to help distend the veins. Inspect and
palpate the veins.
STEP 7
When you are finished with the venipuncture procedure,
simply pull the free end to release the tourniquet.
POINTS OF INTEREST
If a tourniquet is used for preliminary vein selection, do not
leave the tourniquet on for more than one minute. If you
need more time, release it for two minutes then reapply.
Recommended maximum tourniquet time for phlebotomy
procedures is one minute. WHO guidelines give the
maximum time as two minutes.
The following guidelines ONLY apply to practicing:
When practicing you may leave the tourniquet on for
longer as long as one does not make it so tight as to
stop the arterial flow as well which is unlikely. For safety
reasons when practicing do not leave the tourniquet on for
longer than 5 minutes. Take a break for at least 5 minutes
before reapplying.
PROJECT 4B
HOW TO APPLY A TOURNIQUET (TOURNISTRIP)
The Tournistrip is an easy-to-use disposable tourniquet that complies with single-use tourniquet protocol.
INSTRUCTIONS
STEP 1
Remove Tournistrip from box.
STEP 2
Expose the adhesive panel on
Tournistrip (see peel here)
STEP 3
Place Tournistrip around arm with
the printed side facing outward.
STEP 4
Hold the tab end between thumb
and forefinger and pull the slim end
to tighten.
STEP 5
Release Tournistrip.
Pull a Tournistrip from the roll and tear along the perforated
line. You can use the tabs on the box to help further reduce
cross infection.
Slip the slim end through the slot in the wider tab end.
53
PROJECT 4C
HOW TO APPLY A TOURNIQUET (REUSABLE)
Print out the World Health Organizations (WHO) guideline diagram and follow the steps
The main concern with reusable tourniquets is the
possibility of transferring harmful microbes to a patient,
especially if the cleaning recommendations are not
followed to maintain the highest levels of hygiene possible.
Reusable tourniquets if you follow the hygienic handling
recommendations see TOURNIQUETS CLEANING
GUIDE for more information.
The BOA IV constricting band is an innovative reusable tourniquet that is simple to use and makes applying a tourniquet
both effective and simple visit www.NARescue.com for more information.
PROJECT 4D
HOW TO APPLY A TOURNIQUET (BLOOD PRESSURE CUFF)
Apply a blood pressure cuff 7-10 cm (3-4 inches) above the
intended venipuncture site.
Inflate the cuff to about 60 mmHg.
Proceed with the venipuncture procedure.
Deflate as soon as the task is completed (1 minuteno
more than 2 minutes if drawing blood for the lab).
54
PROJECTS 5A 5D
PROJECT 5A
IDENTIFY THE VEINS OF THE UPPER EXTREMITY
INFORMATION
REQUIREMENTS
55
STEP 3
Ask the volunteer to lie down and let the arm hang down
below the rest of the body to allow passive gravitation to
fill the veins with blood. Apply the tourniquet about 5-7
cm (2-3 inches) above the elbow joint
STEP 2
STEP 4
STEP 1
STEP 5
Use the simplified diagram to identify the veins of the
ventral (front) side of your arm by inspection and by
palpation. Use the tips of your middle three fingers.
STEP 6
Use the skin marker pen to draw the veins on the arm.
STEP 7
Figure 10a: The ventral arm veins
STEP 8
Use the skin marker pen to draw the veins on the hand.
Feel free to take a photograph of the venous pattern of
your volunteers arm. You may also want to label the veins
using the diagrams in Step 2.
POINTS OF INTEREST
56
PROJECT 5B
IDENTIFY THE VEINS OF THE LOWER EXTREMITY
INFORMATION
REQUIREMENTS
You will need:
Alcohol hand rub
A pair of clean gloves
A tourniquet
A skin marker pen
A suitable volunteer (A person with a low BMI, male,
athletic, middle-aged, or older with a fair skin will
show the veins more clearly.)
If no volunteer is available, use your own leg
Good lighting
STEP 3
Ask the volunteer to expose the leg area up to some
distance above the knee. The person should be standing,
sitting, or lying down with the leg lower than the rest of
the body to allow passive gravitation to fill the veins with
blood. Apply the tourniquet somewhere at least 10-15 cm
(4-6 inches) above the knee joint.
STEP 2
Have a look at the simplified diagram:
STEP 4
Prepare your hands hygienically and don clean gloves.
(Gloving is optional but strongly recommended.)
See PROJECTS 3A, 3B and 3C
57
STEP 5
Identify the veins, using the simplified diagram, by
inspection and by palpation. Use the tips of your middle
three fingers.
IMPORTANT WARNINGS
STEP 6
Use the skin marker pen to draw the veins on the leg. Feel
free to take a photograph of the venous pattern of your
volunteers leg (of course, with permission). You may also
label the veins using the diagram in Step 2.
POINTS OF INTEREST
There are two types of veins in the legs: superficial veins
and deep veins. Superficial veins lie just below the skin
and are usually visible on the surface. Deep veins are
located much deeper, next to the muscles and arteries
of the leg close to the femur and tibia. Blood flows from
58
PROJECT 5C
OTHER IMPORTANT VEINS (FACE, NECK AND CHEST)
INFORMATION
The external jugular vein has two pairs of valves: the lower pair is located at its entrance into the subclavian vein and
the upper in most cases is about 4 cm above the clavicle. If you position a patient in the Trendelenburg position (body
tilted about 15 with the head lower than the feet) you may notice the external jugular vein pulsating. This is caused by
retrograde pressure from atrial systole (keep in mind that the entrances of the atriums are valveless and the venous valves
are flimsy [SEE VIDEO CLIP].
REQUIREMENTS
You will need:
Alcohol hand rub
A pair of clean gloves
A skin marker pen
A suitable same-gender volunteer (A person with a low
BMI and a fair skin will show the veins more clearly.)
If no volunteer available, use your own neck in the
mirror
Good lighting
59
POINTS OF INTEREST
STEP 1
STEP 2
Have a look at the simplified diagram:
STEP 3
Ask the volunteer to expose the neck area. Place the person
in the Trendelenburg position (head and neck about 15
down), tilt the head slightly to the opposite side, and
apply light pressure just above the clavicle. You can also
ask your volunteer to forcefully exhaling against resistance
(closed lips) so you can see the veins more clearly. This will
increase the intra-thoracic pressure and help to engorge
the external jugular veins. Keep in mind that the course
of the neck veins may vary to some extent; some patients
have double external jugular veins.
STEP 4
Prepare your hands hygienically and don clean gloves.
(Gloving is optional but strongly recommended.)
See PROJECTS 3A, 3B and 3C
STEP 5
Identify the external jugular vein on both sides by inspection
and palpation as it crosses the sternocleidomastoid
muscle as shown in the diagram. Identify the anterior and
posterior jugular veins if possible.
STEP 6
Use the skin marker pen to indicate the course of the vein.
With permission, take a photograph of the venous pattern
of your volunteers neck. You may also label the veins using
the diagrams in Step 2.
60
PROJECT 5D
MAP THE VALVES IN VEINS
INFORMATION
There are valves in most veins; exceptions include the portal, the hepatic, and the internal jugular veins. Venous
valves are bicuspid (two) flap-like structures made of elastic tissue. The valves function to keep blood moving in
one direction only. Once the blood has passed from the arteries through the capillaries, it flows at a slower rate
because little pressure remains to move the blood along towards the heart. In the veins below the heart, blood flow
is facilitated by muscular contraction. When the muscles contract, blood within the veins is squeezed forward in the
vein and the valves open. When the muscle is at rest, the valves close, which helps prevent the backward flow of
blood. This is called the muscle pump.
The direction of venous return in the extremities is from finger and toe tips towards the body
REQUIREMENT
You will need:
Alcohol hand rub
A pair of clean gloves
A skin marker pen
A volunteer (Look for a person with a low BMI,male,
physically fit, middle-aged or older, or with fair skin
to show the veins more clearly.)
If no volunteer is available, use your own arm
Good lighting
METHOD 1
FOLLOW THESE STEPS
STEP 1
Prepare your hands hygienically and don clean gloves
(gloving optional but strongly recommended).
See PROJECTS 3A, 3B and 3C
STEP 3
Occlude the vein distally (on the fingers side) by
applying firm pressure with an index finger. Press your
second index finger next to your first index finger.
Move the second index finger towards the elbow while
exerting mild pressure. This empties the blood from the
lumen as you move your finger along the vein.
STEP 4
Stop at the proximal side of the section identified and
then release the second index finger. The vein will
immediately refill up to the point where a venous valve
is situated. Notice that the previously distended vein
remains flat up to the valve inside the vein. Lift the first
index finger and note the flat section of vein filling up
with venous blood.
STEP 5
Mark the position of the valves with the skin marker pen
and photograph the valve-mapped arm.
STEP 2
Identify a suitable volunteer: someone with clear and
prominent superficial veins on their arms. Ask this
person to make a fist and extend the arm, with the palm
up and slightly below elbow level. Locate a prominent
section of vein of about 10 cm (4 inches) on the inside
of the forearm.
61
METHOD 2
FOLLOW THESE STEPS
STEP 1
Prepare your hands hygienically and don clean gloves.
(Gloving optional but strongly recommended.)
See PROJECTS 3A, 3B and 3C
STEP 2
Have a volunteer make a fist and extend the arm, with
the palm up and slightly below elbow level. Locate a
prominent vein on the inside of the forearm.
STEP 3
Starting near the elbow, run your finger along the vein
STEP 4
Blood will immediately refill the vein up to the point
where a venous valve is encountered and then you will
notice the distended vein remaining flat up to the point
of the valve inside the vein. Lift your finger and notice
how the flat section of vein fills up with venous blood.
STEP 5
Mark the position of the valves with the skin marker
pen. Take a photograph of the valve-mapped arm.
POINTS OF INTEREST
62
PROJECT 6A
PREPARE TO ADMINISTER AN INJECTION
Choose a suitable syringe and needle and draw up medication from various medicine vials
63
IMPORTANT NOTES:
Always keep your and your patients safety as your
first priority!
Use insulin syringes only for insulin.
Too high a dose of insulin may cause a hypoglycemic
coma, or irreversible brain damage. It can even be lethal!
Too low a dose given to a diabetic may lead to a
hyperglycemic coma.
Use safety needles whenever possible according
to the manufacturers instructions and discard in a
dedicated sharps safety container after use.
In all cases when preparing more than one syringe of
different medications or if you are not administering
the medication straight away, clearly label the syringe
above the volume markings the type of medication
and the concentration (e.g., ketamine 100 mg/ml).
Always take great care to avoid needle stick injuries
when working with sharps!
If you need to recap a needle use the one hand
scoop technique (see below).
Never recap a blood-contaminated needle.
Figure 18: The one hand scoop technique for recapping a needle
64
HOW TO DRAW UP
MEDICATION
GENERAL PREPARATION*
HOW TO DRAW UP
MEDICATION FROM A GLASS
VIAL
FOLLOW THESE STEPS
65
IMPORTANT NOTE
REGARDING WITHDRAWING
MEDICATION FOR AN IM
INJECTION
Most training centers recommend a standard twoneedle protocol when performing an IMIthe first
needle for withdrawing the medication and the
second sterile needle for injecting the patient.
Be careful when changing needles. You may use a
conventional needle to draw up the medication, but
use a safety needle when injecting the patient.
A single needle protocol for IMI injections is not
recommended for the following reasons:
The fine, sharp needle tip is easily damaged when
hit against the bottom of the glass vial. This is not
too uncommon and increases pain on subsequent
injection!
The first needle may touch a non-sterile surface and
become contaminated with microbes.
HOW TO DRAW UP
MEDICATION FROM A
GLASS VIAL WITH A RUBBER
MEMBRANE
YOU WILL NEED:
66
HOW TO DRAW UP
MEDICATION FROM A
PLASTIC CONTAINER
SAFETY
Plastic vials are safe from the point of view that it eliminates
the possibility of a sharps injury to the clinician.
On the down side, one can accidentally stick a needle in
an unused vial and unintentionally contaminate it, or stick
the needle right through the container and cause a needle
stick injury.
HOW TO DRAW UP
MEDICATION FROM
A CONTAINER WITH THE
MEDICATION IN POWDER
FORM.
(MANY ANTIBIOTICS COMES
AS A POWDER IN A VIAL.)
SINGLE UNIT
HOW TO DILUTE
MEDICATION IN A 1:10 RATIO
NOTE:
It is safer to administer a medication that has potentially
serious or even life threatening side effects by diluting it
and injecting it slowly!
67
POINTS OF INTEREST
STUDY THE CDC GUIDELINES FOR INJECTION
SAFETY:
What is injection safety?
Injection safety, or safe injection practices, is a set of
measures taken to perform injections in an optimally safe
manner for patients, healthcare personnel, and others. A
safe injection does not harm the recipient, does not expose
the provider to any avoidable risks, and does not result in
waste that is dangerous for the community (e.g., through
inappropriate disposal of injection equipment). Injection
safety includes practices intended to prevent transmission
of infectious diseases between one patient and another,
for example between a patient and healthcare provider,
and also to prevent harm such as needlestick injuries.
What is aseptic technique?
In this context, aseptic technique refers to the manner
of handling, preparing, and storing of medications and
injection equipment/supplies (e.g., syringes, needles and
IV tubing) to prevent microbial contamination.
What are some of the unsafe injection practices that
have resulted in transmission of pathogens?
The most common practices that have resulted in
transmission of hepatitis C virus (HCV), hepatitis B virus
(HBV), and/or other pathogens include:
Using the same syringe to administer medication
to more than one patient, even if the needle was
changed or the injection was administered through
an intervening length of intravenous (IV) tubing;
Accessing a medication vial or bag with a syringe that
has already been used to administer medication to a
patient then reusing contents from that vial or bag for
another patient;
Using medications packaged as single-dose or singleuse for more than one patient;
Failing to use aseptic technique when preparing and
administering injections.
68
PROJECT 6B
HOW TO PREPARE THE PUNCTURE SITE ASEPTICALLY
(ROUTINE VENIPUNCTURE)
A simple but essential step to prevent infections
INFORMATION
The skin harbors, in large numbers, a variety of bacterial
species, as well as other microorganisms. These microbes
(also called flora) can be divided into two groups:
resident flora (lives in and on the skin) and transient flora
(temporary visitor microorganisms). Resident and transient
flora do not normally cause diseases on the skin but if they
enter the body they may cause diseases. For example,
Staphylococcus epidermidis lives quite innocently on the
skin in great numbers but may cause sub-acute bacterial
endocarditis (SBE) under certain conditions if they enter
the blood stream.
It is impossible to sterilize (kill all known microorganisms
and spores) on a patients skin but one can reduce and
REQUIREMENTS
You will need:
Antiseptic soap for handwashing or alcohol hand rub
A clean work surface cover
A tourniquet (re-usable)
Clean gloves
Alcohol prep swabs
69
POINTS OF INTEREST
STEP 1
STEP 2
Prepare your hands hygienically (wash or alcohol hand
rubPROJECT 3A/B). Choose a venipuncture site, apply
a tourniquet, and select a suitable vein.
STEP 3
Tear open an alcohol prep sachet and place on the work
surface.
Put on clean gloves and remove the alcohol-saturated square.
STEP 4
Cleanse in a circular fashion for 30 seconds beginning at
the intended puncture site then make circular motions
outwards (see the diagram below).
STEP 5
The next step will be performing the venipuncture
procedure (PROJECTS 11A, B, C and D) for the purpose
of this project you may now remove the tourniquet.
70
IMPORTANT:
Adhering to a meticulous sterility and aseptic protocol will
dramatically reduce the number of infective complications
that your patients could experience. Insignificant
deviations from the recommended protocol make a big
difference!
Ensure that the alcohol prep square is saturated with clear
alcohol. If the square is dry or semi-dry, cloudy or colored,
then discard it and use a new one.
PROJECT 6C
HOW TO PREPARE THE PUNCTURE SITE ASEPTICALLY
(COLLECTING BLOOD FOR BLOOD CULTURE)
Minimize the chances of contaminant bacteria of entering the blood culture bottle
This project is for your information only as there are no blood culture bottles supplied in the kit.
Labs usually supply dedicated cleaning kits for the purpose of aseptically preparing the puncture site before taking blood
samples for culturing.
STEP 1
STEP 5
STEP 2
Use a 2% chlorhexidine gluconate in 70% alcohol solution,
as well as 3-6 sterile swabs opened onto the sterile field.
Alternatively a 2% iodine tincture or 10% povidone iodine
may be used in place of the chlorhexidine gluconate and
alcohol solution.
STEP 3
Remove the cap of each blood culture bottle and use a
non-touch technique to scrub the vial stoppers well with a
fresh chlorhexidine and 70% alcohol swab. Allow these to
dry for 30 seconds.
STEP 4
Ensure that all the items and equipment for drawing blood
are ready and prepared.
STEP 6
Perform hand hygiene for the second time.
STEP 7
Put on CLEAN gloves (do not touch the venipuncture site
after skin preparation. If palpation is absolutely necessary
then STERILE GLOVES must be used prior to palpation).
STEP 8
Using swabs saturated with 2% chlorhexidine in 70%
alcohol, disinfect the venipuncture site in a scrubbing
motion. Perform 2-3 scrubs using a fresh swab for each
scrub, with the last scrub starting at the intended puncture
site and spiraling out in a circular motion towards the
periphery. Clean for a total of 1-2 minutes, and then allow
the site to dry for approximately 30 seconds.
(If tincture of iodine is used, remove with 70% ethanol after
the procedure.)
71
STEP 9
POINTS OF INTEREST:
72
PROJECT 6D
HOW TO PREPARE THE PUNCTURE SITE ASEPTICALLY
(COLLECTING BLOOD FROM BLOOD DONOR)
Prevent bacterial contamination of donated blood
ONE-STEP PROCEDURE
TWO-STEP PROCEDURE
73
CASE STUDY 3:
MY LIFE CHANGED DRASTICALLY IN A SPLIT SECOND
76
PROJECT 7A
DRAW CAPILLARY BLOOD ADULT
A useful method of sampling a couple of drops of blood
VIDEO
The student is allowed to perform this project under supervision of a suitably qualified medical professional. If
you have friend or family member who is a diabetic then offer to test their blood glucose level.
INFORMATION
Blood from a finger stick differs from blood collected from
a vein in the fact that it is a mixture between venous,
(mainly) capillary and arterial blood, as well as minute
amounts of tissue fluid.
If properly executed, blood collected from a finger (or
heel stick) will offer surprisingly accurate bio-chemical
information. Keep in mind that the following readings may
be slightly different:
Lower concentrations of potassium, total protein, and
calcium.
Higher glucose.
77
REQUIREMENTS
STEP 6
STEP 1
Open the above items on the clean work surface cover.
STEP 2
Greet the patient, introduce yourself and positively identify
the patient.
Do short medical history (allergies, bleeding tendencies,
and anticoagulant medication). Verify the patients status
regarding fasting, dietary restrictions, medications taken
(and time), and other relevant information. Properly fill out
and make appropriate notes on the lab requisition form
including the specific tests requested.
STEP 3
Prepare your hands hygienically.
STEP 4
The patient should be comfortably positioned sitting or
lying down. Extend the patients arm, keeping the hand
relatively open.
Choose a suitable puncture site. Use the pads of the
middle or fourth fingers of the non-dominant hand and
somewhat to the side of the finger pad.
Avoid:
The thumb, index finger, as well as the fifth fingers if
possible
The tip-area and central pad area of the finger
Puncturing a finger that is cold or cyanotic, swollen,
scarred, or covered with a rash
STEP 5
Ensure that the fingertip is clean with no visible dirt. Wipe the
fingertip with an alcohol prep swab. Wait 30 seconds to air dry.
78
STEP 7
Wipe away the first drop of blood which may contain
excess tissue fluid.
STEP 8
STEP 9
Cap then rotate and invert the collection container to mix
the blood collected.
STEP 10
Have the patient hold a small gauze pad over the puncture
site for a couple of minutes to stop the bleeding.
STEP 11
Dispose of contaminated materials in their designated
containers.
Important note: All lancets are single-use only and
must be disposed of in an approved sharps container
immediately after use.
STEP 12
Label all appropriate tubes at the patient bedside and
deliver specimens promptly to the laboratory.
POINTS OF INTEREST
A number of disposable spring-loaded skin puncture
devices are available that will ensure a safer procedure. The
spring-load mechanism should be pre-activated. The lancet
will automatically puncture the skin when the auto-stab
mechanism is released, and will then immediately retract
back into the housing of the device. This virtually eliminates
the possibility of accidental needlestick injury to the medical
professional performing the procedure.
Regular venipuncture tubes generally hold 5-10 ml (adult)
and 2-4 ml (pediatric) of blood. Tubes for finger sticks or heel
sticks generally hold one-half ml or less.
PROJECT 7B
DRAW CAPILLARY BLOOD BABY
A useful method of sampling a couple of drops of blood
VIDEO
Figure 6: A surgeon scrubbing before surgery
INFORMATION
Warning: This project is intended for your information only. Under no circumstances may you use a baby to
practice this technique!
A blood sample obtained from a heel puncture is a useful and simple way of collecting a blood sample from a newborn
baby up to about 6 months of age.
It is commonly used for the following purposes in
babies:
Metabolic and genetic screening tests
Bilirubin levels (to monitor jaundice of the newborn)
Blood glucose and Lactate analysis
REQUIREMENTS
79
STEP 2
Check the patient for correct identity.
Check the medical history with the babys parent (allergies,
bleeding tendencies, medication, etc.). Verify the patients
status regarding the physicians specific orders. Properly
fill out and make appropriate notes on the lab requisition
form including the specific test(s) requested.
STEP 3
Ensure that the baby is lying comfortably, warm and
secure (for example, safely on a bed or on a persons lap).
Ask a parent or a nursing professional to assist by passively
restraining the baby.
Wash your hands. See handwashing guidelines and put on
clean gloves (PROJECT 3A, 3B and PROJECT 3C).
Clean the site with warm water/saline and gauze or cotton
wool. Do not use alcohol wipes to clean the skin of a baby.
STEP 4
Hold the babys heel with the non-dominant hand. It
may be necessary to compress the foot beforehand to
get a good flow of blood. With the foot flexed (see Fig.
14), prick the heel, preferably with a loaded automated
lancet or with a disposable lancet, to a depth of 1-2mm in
the plantar surface of the heel (see Fig. 15). The puncture
should be made perpendicular to the heel-print ridges. If
you must use a regular lancet, then use a sterile single-use
lancet to stab the heel at 90 to the skins surface in a single,
brisk stabbing movement.
STEP 5
Gently but firmly compress the babys heel (avoid excessive
pressure). Release the tension, wipe away the first drop of
blood, and then re-apply the tension to allow the blood to
collect in globules, which can then be collected into the
blood bottle.
STEP 6
Cap then rotate and invert the collection container to mix
the blood collected.
STEP 7
Apply pressure to the site with gauze and maintain the
pressure until bleeding has stopped. Tape a small piece
of gauze or cotton wool over the puncture site using
hypoallergenic tape.
STEP 8
Dispose of contaminated materials in designated
containers.
Important note: All lancets are single-use only and must be
disposed of in an approved sharps container immediately
after use.
STEP 9
Take a consecutive blood sample from the alternate heel.
Vary the puncture site positions.
STEP 10
Figure 20: How to hold the heel when performing a heel prick procedure
80
STEP 11
Label all appropriate tubes at the patient bedside and
deliver specimens promptly to the laboratory.
POINTS OF INTEREST
To avoid irritating, and even possible mutilating
complications the operator should adhere to a very strict
procedural protocol (as above).
Complications that can arise in capillary sampling
include:
PROJECT 8
HOW TO GIVE A SUBCUTANEOUS INJECTION
Deposit medication in the subcutaneous layer of the skin
VIDEO
*If you have an insulin dependent diabetic friend or family member, offer to administer their next insulin
subcutaneous injection. Strictly follow the physicians orders!
INFORMATION
The skin is made up of different layers. Underneath the
epidermis and dermis, which contain sweat glands and
hair follicles, is a layer of fat. Subcutaneous injections are
given into this area. As a general rule, suitable areas for
subcutaneous injections are those areas with a substantial
amount of fat below the skin, for example, the thighs,
buttocks, and abdomen.
81
REQUIREMENTS
You will need:
Alcohol wipe
Clean work surface cover
Clean gloves
Ampule of medication
Small syringe (0.5 ml)
30 gauge needle (preassembled on syringe)
Cotton wool or gauze
The Venipuncture Trainer
82
STEP 2
Follow the steps described in Project 6A to draw up the
medication for SQ injection.
STEP 3
Choose the injection site for this dose and expose the
area (if working on a real patient). There are several areas
STEP 4
Prepare your hands hygienically and don clean gloves
(sterile gloves are not required).
STEP 5
Open an alcohol prep swab and wipe the intended area
for SQ injection in a circular motion and allow to air dry.
STEP 6
Spread the index finger and thumb of the non-dominant
hand about 5 cm (2 inches) apart and place them on either
side of the planned injection spot. Pinch (bunch-up) the
skin in the chosen injection area between your thumb and
index finger.
Warnings: Stay clear of your own fingers. Take great
care not to prick your own finger!
Use a safety needle in a clinical setting.
Note: In a home-care situation, no aseptic preparation
of the skin is required, presuming reasonable personal
hygiene. When a medical professional gives the SQ
injection, skin prepping is advisableespecially in a clinic
or hospital where medical professionals need to maintain
a sterile chain, unlike the home-care situation.
STEP 7
Continue to hold the skin and insert the needle into
the skin in the center of the skin fold so that the needle
is at an angle of 45-90. *Aspirate if your hospital/unit
recommends aspiration when giving a subcutaneous
injection.
STEP 8
Push the syringe plunger to inject the medicine. It is
recommended that you count slowly from 1 to 10 while
injecting the medication.
Remove the needle from the skin and release the skin.
*Comments:
As a general rule, aspirating before injecting is a good
habit and is thus recommended.
How to aspirate:
With the needle in the injection site, gently
withdrawing the plunger, exerting negative
pressure before injecting the medication. This is
done to make sure that you are not in a blood vessel,
and thus administering an inadvertent IV injection
and causing a possible medical emergency!
STEP 9
Press a piece of cotton wool or gauze lightly over the
injection site for a couple of seconds (optional). Do not
massage the injection site.
STEP 10
Discard the used syringe and needle in the sharps waste
container. Do not recap or remove the needle! Remove
gloves and discard in suitable medical waste bin.
STEP 11
Mark the puncture site, date, and time of the injection on a
suitable chart or diagram.
STEP 12
Simulate the project by following Steps 1-10 using the
unassembled Venipuncture Trainer. Do not inject in the
blood vessel areas.
Use 2ml of air or withdraw 2ml of fluid from the In of one
of the IV fluid bags to use as medication when doing the
simulation exercise.
83
POINTS OF INTEREST
Safe areas for giving subcutaneous injections:
Abdomen: Uncover the abdomen from about 5 cm
(2 inches) below the umbilicus up to the waist area. You
may give a shot below the waist, to just above the hipbone,
and from where the body curves at the side to about
5 cm (2 inches) from the middle of the abdomen. Avoid
the umbilicus.
Thigh: Uncover the leg from the knee to the hip. The midsection of the thigh, from mid-front to mid-side on the
outside area of the thigh, is a safe site. Gently, grasp the
area with index finger and thumb to ensure that you can
pinch one to two inches of skin.
Upper Arm: Uncover the arm to the shoulder. Have the
patient stand with hand on hip. Stand to the side, slightly
behind the patient. Find the area halfway between the
elbow and shoulder. Gently grasp the skin at the back of
the arm between your thumb and first two fingers. You
should have 1-2 inches of skin.
Various other areas see Figure 23.
When a patient receives multiple injections over a period
84
PROJECT 9
HOW TO GIVE AN INTRAMUSCULAR INJECTION
Deposit medication in a suitable muscle
VIDEO
Figure 6: A surgeon scrubbing before surgery
INFORMATION
Medication is injected well into the muscle layer below the
various layers of skin.
REQUIREMENTS
85
SIMULATE A
SUBCUTANEOUS INJECTION
USING THE VENIPUNCTURE
TRAINER. SEE STEP 12.
FOLLOW THESE STEPS AS
IF IN A REAL-LIFE CLINICAL
SITUATION:
STEP 1
Greet and positively check the patients identity.
Check the following on the medication ampule or vial
before proceeding:
The correct medication, name, amount (or volume,
e.g., 10 ml), and concentration (e.g., 5 mg/ml).
The expiration date. Never use expired medication.
Abnormalities regarding consistency and color of the
medication in the container. If in doubt, send back to
the pharmacy.
STEP 2
Follow the step-by-step instructions on withdrawing
medication for an IM injectionsee PROJECT 6A.
STEP 3
Prepare your hands hygienically and don clean gloves
(sterile gloves not required).
STEP 4
Choose the injection site for the dose and expose the area
(if working on a real patient). There are several areas of
the body suitable for giving intramuscular injections (IMI),
shown on the diagrams.
STEP 5
Swab the injection site with an alcohol pad for 30 seconds
in a circular outward motion, up to 5 cm around. Allow
the alcohol to air dry (30 seconds). Spread the index finger
and thumb of the non-dominant hand about 5 cm (2
inches) apart and place them on either side of the planned
injection spot. Gently spread your index finger and thumb
to tense the tissue. Ask the patient to relax and then to
take a deep breath. As the patient inhales, make a quick
dart-like motion to insert the needle at a 90 angle to an
appropriate depth, ensuring that the needle tip enters the
muscle properly.
Warning: Stay clear of your own fingers. Take great care
not to prick your own finger!
86
STEP 6
It is essential to aspirate before depositing the medication
into the muscle. To do this, gently withdraw the plunger
before injecting the medication into the muscle, in order
to avoid an inadvertent intra-vascular injection and thus a
possible medical emergency.
If you aspirate blood, withdraw the needle and prepare a
new syringe.
STEP 7
If no blood is aspirated, continue to hold the skin and
push the syringe plunger to inject the medicine. It is
recommended that you count slowly from 1 to 10 for
every milliliter (ml) being injected.
STEP 8
When all of the medication has been injected, promptly
withdraw the needle and apply pressure to the injection
site using a gauze square to prevent bruising or a
hematoma; this will also minimize medication seeping into
the subcutaneous space. Certain medications may require
massaging of the injection area for a minute or two, but
for others this may be contraindicated. Apply an adhesive
bandage strip if necessary.
STEP 9
Discard the used syringe and needle in a designated
sharps waste container. Do not recap or remove the
needle! Remove gloves and discard in a suitable medical
waste bin.
STEP 10
Document the time, medication, dose, route, site, and
patients response to injection.
STEP 11
Perform the project by following Steps 1-10 using the
unassembled Venipuncture Trainer. Do not inject in the
blood vessel areas.
Withdraw 2ml of air or withdraw 2ml of fluid from the In
of one of the IV fluid bags as medication when doing the
simulation exercise.
POINTS OF INTEREST
Relatively safe sites for IM injection are the deltoid, dorsal gluteal, ventrogluteal, and vastus lateralis (especially for children
under two years of age).
Deltoid Muscle: The deltoid muscle is located in the
upper arm, just below the shoulder. To mark this site, place
the palm of your hand on the shoulder and spread your
thumb away from the four fingers in an upside down
V shape. Ensure that the middle of the patients arm is
centered in your V. You will want to give the injection into
the middle of this V.
Figure 26a and b: Giving an intramuscular injection in the vastus lateralis muscle
Figure 28a and b: Giving a intramuscular injection in the dorsal gluteus
muscle
87
PROJECT 10 A
SET UP THE VENIPUNCTURE TRAINER FOR PHLEBOTOMY
How to prepare the trainer for performing various practical projects
VIDEO
INFORMATION
REQUIREMENTS
88
STEP 7
You are ready to start with the phlebotomy projects.
(PROJECTS 11-14)
stand
STEP 2
Inspect the IV fluid bag. Note the two ports marked as
In and Out.
IV bag
STEP 3
in port
out port
drip chamber
STEP 4
Connect the adult IV line to the bag using the Out
port. Connect the other end to one of the tubes
(Venipuncture Trainer veins) using a connector. Ensure
that the connection is secure and doesnt leak.
IV line
flow-control clamp
STEP 5
Place or suspend the IV-fluid bag about a meter ( a
yard) above the work surface. Suspend it from a hook
or nail in the wall or place it on a shelf above the work
surface.
connector
IV Trainer
simulation veins
STEP 6
Keep the lumen stoppers nearby. Place a shallow
container at the outflow end of the Venipuncture
Trainer. Open the infusion flow-speed mechanism. Press
and release the drip chamber once or twice to fill it
lumen stoppers
PROJECT 10 B
SET UP THE VENIPUNCTURE TRAINER FOR IV PROJECTS
For PROJECT 11D HOW TO SETUP AN IV LINE, you will have to modify the Venipuncture Trainer setup as follows:
Close or occlude the near side of both veins with the lumen stoppers.
Place a shallow container (e.g., a kidney dish) at the far, open ends of the veins to receive the IV fluid following
a successful venipuncture procedure.
Proceed with PROJECT 11D.
89
PROJECT 10 C
SET UP THE VENIPUNCTURE TRAINER FOR ARTERIAL BLOOD
For PROJECT 12C DRAW ARTERIAL BLOOD you will have to modify the Venipuncture Trainer setup as follows:
Fill a 10 ml syringe with fake blood and connect to the smaller vein, and let it run through the tube.
Now tightly occlude one side of the smaller vein.
Ask another person to sit opposite you and then to press down on the plunger of the syringe. They can use a moderate
pulsing pressure to simulate the pulsations of an artery.
Perform the simulation procedure for drawing arterial blood PROJECT 12C.
THERAPEUTIC PHLEBOTOMY
(BLOODLETTING)
MAXIMUM ALLOWABLE
TOTAL BLOOD DRAW
VOLUMES
The maximum allowable total blood draw volumes
depends on the patients body weight, blood Hb
(Hemoglobin) level, and the general condition at the time
of the draw.
[CLICK HERE] to see and print the Table for Maximum
Allowable Total Blood Draw Volumes.
90
PROJECT 11A
DRAW VENOUS BLOOD USING A VACUUM TUBE
One of a number of methods to draw venous blood
VIDEO
INFORMATION
It is strongly recommended that the student read the WHO
guidelines on drawing blood: Best Practices in Phlebotomy
before proceeding with this project.
Notes on choosing a venipuncture site:
The most commonly used veins are the larger and
easily accessible median cubital or cephalic veins of
the arm, followed by the basilic vein on the dorsum of
the arm or dorsal hand veins.
The veins of the foot are a last resort because of the
higher probability of complications.
Other veins, like the external jugular vein, are rarely
used.
A good vein will be both visible and palpable.
However, occasionally you may have to depend only
on your sense of palpation.
To make it easier to see the veins, warm the arm for 10
minutes with a hot pack or let the hand hang down.
If you feel a pulse when palpating the blood vessel,
you may be looking at a superficial artery. Inspect the
area further to identify a definite vein.
Avoid inserting the catheter into a bifurcation (where
the vein splits) or near large valves.
91
REQUIREMENTS
You will need:
Laboratory specimen labels (only in clinical setting)
Pen for writing
Laboratory forms
The sharps waste container
Alcohol wipe
Clean gloves
Clean work surface cover
A Vacutainer holder (hub)
A Vacutainer needle
Vacutainer tubes
An Autosafe Safety Phlebotomy Device (safety
needle preassembled on hub/holder)
Cotton wool or gauze
Strapping (adhesive bandage strip)
A fully set up Venipuncture Trainer
See PROJECT 10A
A phlebotomist will also need leak-proof transportation
bags and containers (not supplied in kit).
IMPORTANT NOTE:
92
STEP 2
Open a clean work surface cover. From the REQUIREMENTS
list above, gather the relevant items and equipment (open
outer plastic wrapping) and place on this cover. Set out
all of the tubes you will need by the order of the draw*
and have any necessary tools (tourniquet, alcohol swabs,
sharps waste container, and biohazard waste bag) nearby.
Tear open the alcohol prep sachet.
Assemble the Vacutainer by attaching the Vacutainer
needle to the Vacutainer hub or use the Autosafe Safety
Vacutainer Phlebotomy Device (with needle preassembled
to the hub).
STEP 3
Perform hand hygiene. See PROJECT 3A A TECHNIQUE
FOR PROPER HANDWASHING and PROJECT 3B
CLEANING HANDS WITH AN ANTISEPTIC RUB.
STEP 4
Select the site, preferably at the cubital (antecubital) area
(i.e., the inner bend of the elbow).
Locate the anatomic landmarks. Inspect and palpate the
veins in the intended venipuncture site. If necessary, one
may apply a tourniquet temporarily, about 4-5 fingers
above the intended venipuncture site in order to facilitate
the inspection and palpation. Remove the tourniquet until
ready to proceed.
Note: To make it easier to see the veins, warm the arm for
10 minutes with a hot pack or let the hand hang down.
STEP 5
When ready for drawing the blood, apply the tourniquet
about 4-5 finger widths above the selected venipuncture
site.
STEP 6
Ask the patient to form a fist to make the veins more
prominent.
STEP7
Put on clean (non-sterile) gloves.
STEP 8
Disinfect the site using 70% isopropyl alcohol for 30
seconds and allow it to dry completely (30 seconds).
DO NOT touch the site again once the alcohol (or other
antiseptic) has been applied! If you must touch it again to
feel the vein, touch a clean alcohol pad first to aseptically
treat your glove.
STEP 9
Anchor the vein by holding the patients arm and placing
the thumb of the opposite hand below the venipuncture
site.
DO NOT insert the needle where veins are branching as
this will increase the chance of a hematoma.
STEP 10
Enter the vein swiftly at a 15-30 angle. Ensure that the
bevel of the needle is pointing up. DO NOT bend the
needle.
Note: Angling the needle less than 15 increases the
chance of the needle staying above the vein and an angle
of more than 30 increases the chance of penetrating the
deep wall of the vein.
STEP 11
Support the Vacutainer tube holder, then push the
Vacutainer tube into the hub and check for blood flow.
Allow the tube to fill and then remove it from the tube
holder, all the time gently supporting the tube holder to
prevent the needle from slipping out of the vein. When
blood flow stops, remove the tube by holding the hub
securely and pulling the tube off the needle.
STEP 13
Withdraw the needle gently and then give the patient a
clean gauze or dry cotton-wool ball to apply to the site
with firm pressure for 2-3 minutes to achieve hemostasis
and avoid a hematoma. Ensure that the patient has
stopped bleeding and then apply tape and gauze to the
venipuncture site.
STEP 14
Discard sharps (e.g., the used needle/s and broken glass
and syringe or blood-sampling device) into a punctureresistant sharps container. Place other items like used
gloves and all items contaminated with blood or body
fluids into the infectious waste.
STEP 15
Check the labels and forms for accuracy.
STEP 16
Perform hand hygiene.
STEP 17
Simulate the project by following Steps 1-16 using the fully
setup Venipuncture Trainersee PROJECT 10A.
Ensure that the tubes are filled with fake blood, that the IV
fluids tubing flow-speed regulation device is set on open,
and that the ends of the tubes have lumen stoppers in
position.
STEP 12
If tube used has additives mix the contents by gently
inverting the tube 5-8 times (do not shake). Fill the
remaining tubes and repeat the mixing routine after each
tube has been filled.
Once sufficient blood has been collected, release the
tourniquet BEFORE withdrawing the needle.
Note:
When filling additional tubes determine what tests
are ordered and what tubes will be necessary *BEFORE
you begin drawing blood, follow the correct sequence
of drawing blood for these tubes (SEE TABLE 3).
93
POINTS OF INTEREST
Tubes
Additives
Tests
Yellow
SPS
Blue
Sodium Citrate
Red
Green
Ammonia Level
Light green
Lavender
EDTA
Pale yellow
Gray
Table 3: *Recommended order of draw for plastic vacuum tubes (may differ slightly from your hospitals protocol).
DO
DO NOT
DO discard the used device (a needle and syringe is a single unit) immediately into a robust sharps container
94
ADDITIONAL SAFETY
RECOMMENDATIONS:
VIDEO
95
PROJECT 11 B
DRAW VENOUS BLOOD USING A SYRINGE AND NEEDLE
One of a number of methods to draw venous blood
VIDEO
INFORMATION
REQUIREMENTS
96
STEP 2
Open a clean work surface cover then gather and open the
relevant items and equipment. Assemble equipment (see
REQUIREMENTS above). Set out all of the tubes you will
need and have all the necessary tools (e.g., tourniquet and
alcohol swabs) nearby. Tear open the alcohol prep sachet.
The needle should be 21g or wider in order to minimize
hemolysis. ALL needles and syringes are single-use only.
Briefly inspect the needle, especially the tip, to ensure it is
sharp and undamaged.
Remove the syringe from the packaging and insert the
nozzle of the syringe firmly into the exposed hub of the
capped hypodermic needle. Move the plunger within the
barrel to ensure free movement.
Note: Use safety needles in all clinical settings. When
simulating the procedure you may use a regular needle
but take great care not to injure yourself.
STEP 3
Perform hand hygiene.
See PROJECT 3A A TECHNIQUE FOR PROPER
HANDWASHING and PROJECT 3B Cleaning hands
with an antiseptic rub.
STEP 4
Select the site, preferably at the cubital area.
Locate the anatomic landmarks. Inspect and palpate the
veins in the intended venipuncture site. One may apply a
tourniquet temporarily, if necessary, 4-5 fingers above the
intended venipuncture site to facilitate the inspection and
palpation. Remove the tourniquet until ready to proceed.
Note: Warming the arm with a hot pack or hanging the
hand down may make it easier to see the veins.
STEP 5
When you are ready to draw blood, apply the
tourniquet about 45 finger widths above the selected
venipuncture site.
STEP 6
Put on clean (non-sterile) gloves.
STEP 7
Ask the patient to form a fist so that the veins are more
prominent.
Important note: Dont ask the patient to pump his/her fist.
STEP 8
Disinfect the site using 70% isopropyl alcohol for 30
seconds and allow it to dry completely (another 30
seconds).
DO NOT touch the site again once the alcohol (or other
antiseptic) has been applied! If you must touch it again to
feel the vein, touch a clean alcohol pad first to aseptically
treat your glove.
STEP 9
Anchor the vein by holding the patients arm and
placing the thumb of the opposite hand below the
venipuncture site.
Do not insert the needle where veins are branching, as this
will increase the chance of a hematoma.
STEP 10
Enter the vein swiftly at a 15-30 angle. Ensure that the
bevel of the needle is pointing up. D NOT bend the
needle. Hold/stabilize the barrel of the syringe and gently
withdraw the plunger until the required amount of blood
has filled the syringe.
Note: Angling the needle less than 15 increases the
chance of the needle staying above the vein and an angle
of more than 30 increases the chance of penetrating the
deep wall of the vein.
97
STEP 11
STEP 15
STEP 12
Withdraw the needle gently and place clean gauze or a
dry cotton-wool ball with strapping to the site. Apply firm
pressure to achieve hemostasis and avoid a hematoma.
Inspect the site after 2-3 minutes to ensure that the patient
has stopped bleeding.
STEP 13
**Always use a safety transfer device for transferring blood
from a syringe to the vacuum tubes or the blood culture
bottles. [Click here] for more information on the method
of transferring blood with a safety device.
If no safety transfer device is available, place the vacuum
tubes in a test tube rack before inserting the needle into
the vacuum tube. Carefully penetrate the needle through
the tubes stopper and let the blood passively fill the tubes.
Warnings when transferring blood:
Do not hold vacuum tube in your hand!
Do not exert pressure on the plunger of the syringe.
This ensures that you avoid hemolysis or causing the
needle or stopper to pop off thus creating a spray of
blood droplets with the danger of exposing you and
others to bloodborne pathogens.
STEP 14
If the tube used has additives, mix the contents by gently
inverting the tube 5-8 times (do not shake). Fill the
remaining tubes and repeat the mixing routine after each
tube has been filled.
STEP 16
Recheck the labels and forms for accuracy.
STEP 17
Perform hand hygiene.
STEP 18
Simulate the project by following Steps 1-17 using the
fully setup Venipuncture Trainersee PROJECT 10 A.
Ensure that the tubes are filled with fake blood, that the IV
fluids tubing flow-speed regulation device is set on open,
and that the ends of the tubes have lumen stoppers in
position.
POINTS OF INTEREST:
Comments on safety and avoiding injury to you and
your patient
Students of phlebotomy should be aware of the most
recent information regarding the safety aspects related to
safety needles and other devices by visiting the following
websites:
*WHO (World Health Organization) http://whqlibdoc.
who.int/publications/2010/9789241599221_eng.pdf
**OHASA (Occupational Safety and Health Administration)
http://www.osha.gov
USA: Safety holders (preferably disposable), safety
needles, safety blood transfer devices, and shields are
mandatory, regardless of the blood collecting system
used. See Occupational Safety and Health Agency
(OSHA) guidelines.
Other countries: visit your countrys governmental
Occupational Health and Safety Department.
98
PROJECT 11C
DRAW BLOOD USING A BUTTERFLY NEEDLE
One of a number of methods to draw venous blood
VIDEO
INFORMATION
Winged infusion sets, commonly known as butterfly
infusion sets or butterfly needles, are frequently used to
perform venipuncture procedures. They are especially
useful when doing venipuncture on patients with spastic,
thin, or rolling veins. Winged needles are most commonly
used when the available veins are very small, fragile, and
difficult to access or when veins are in a location that
would make a standard evacuated tube system difficult to
use. Winged needles are also used on very shallow veins
because the design allows the needle to be inserted at a
much shallower angle (10-15) compared to a standard
evacuated tube system. Winged needles are nearly always
used when drawing blood from the hand, wrist, or other
places where veins are very close to the skin.
Due to the fact that the winged needle is attached to a
flexible tube, there is less chance of the needle slipping out
or perforating the deep end of the vein. This can happen
if either the patient or the phlebotomist moves during the
procedure, especially when drawing blood for multiple
tubes. Winged needles are usually 21g (green label) or 23g
(blue label). Rarely, a 25g (orange label) is used, mostly in
pediatrics or in very difficult cases; a needle of such small
diameter may cause hemolysis, thus invalidating test
results.
The needle is held by the wings and placed into the vein,
generally at a fairly shallow angle. The wings allow the
phlebotomist to grasp the needle very close to the end
to ensure accurate insertion into a vein. When the needle
enters the lumen of a vein a flash of blood can be seen. The
flash is a small amount of blood that flows back into the
tubing when the needle enters a vein. The phlebotomist
can then push vacuum tubes into the hub or use a syringe
to draw blood.
REQUIREMENTS
You will need:
Laboratory specimen labels
Pen for writing
Laboratory forms
The sharps container
Alcohol wipe
Clean gloves
Clean work surface cover
Winged infusion set21 gauge
Syringe (10ml or 20ml)
Cotton wool or gauze square
Strapping (adhesive bandage strip)
Fully setup Venipuncture Trainersee PROJECT 10A
Phlebotomist, in addition to the above items, will also
need leak-proof transportation bags and containers.
99
STEP 2
Assemble equipment (see REQUIREMENTS above). Set
out all of the tubes needed and have all the necessary
tools (tourniquet and alcohol swabs, etc.) nearby. The
butterfly needle should be a 21 gauge or wider in order to
minimize hemolysis. ALL needles and syringes are singleuse only. Open the outer packaging of all items to be used,
including the butterfly needle and syringe.
Insert the nozzle of the Vacutainer or the syringe firmly
into the hub end of the butterfly needle tubing.
Note: Use safety butterfly needles in all clinical settings.
When simulating the procedure you may use a regular
needle but take great care not to prick yourself.
STEP 3
Perform hand hygiene.
See PROJECT 3A A technique for proper handwashing
and PROJECT 3B Cleaning hands with an antiseptic rub
STEP 4
Select the site, preferably at the cubital (antecubital) area.
Locate the anatomic landmarks. Inspect and palpate the
veins in the intended venipuncture site. One may apply a
tourniquet temporarily, if necessary, 4-5 fingers above the
intended venipuncture site to facilitate inspection and
palpation. Remove the tourniquet until ready to proceed.
Note: Warm the arm with a hot pack or hang the hand
down to make it easier to see the veins.
STEP 5
When ready to draw the blood sample, apply the
tourniquet about 4-5 finger widths above the selected
venipuncture site.
100
STEP 6
Put on clean (non-sterile) gloves.
STEP 7
Ask the patient to form a fist to make the veins more
prominent.
Important note: Dont ask the patient to pump the fist.
STEP 8
Disinfect the site using 70% isopropyl alcohol and allow to
dry completely (clean for 30 seconds and allow to dry for
another 30 seconds).
Do not touch the site again once the alcohol (or other
antiseptic) has been applied! If you must touch it again to
feel the vein, first touch a clean alcohol pad to aseptically
treat your glove.
STEP 9
Anchor the vein by holding the patients arm and placing the
thumb of the opposite hand below the venipuncture site.
DO NOT insert the Butterfly needle where veins are
branching as this will increase the chance of a hematoma.
STEP 10
Use the butterfly needle to enter the vein with a swift
movement at a 15 to 30 angle. Ensure that the bevel of
the needle is pointing up. DO NOT bend the needle. Keep
your eyes open for the flash-back of blood appearing in
the tube lumen indicating a successful venipuncture.
STEP 11
Draw blood by using either a Vacutainer or a syringe
connected to the butterfly tubing (some winged
needles have the Vacutainer hub pre-attached). See
PROJECT 11A or PROJECT 11B.
Note: If you have to draw a tube for a coagulation
specimen (citrate/light blue top) as the first specimen,
then draw blood using a clear top* (no additive) vacuum
tube before the citrate tube in order to fill the empty
tube space with blood, thereby ensuring the proper
blood-to-additive ratio (discard this tube after use).
*Preferably a clear top but any other color top tube will be
good.
STEP 12
Once you collect sufficient blood, ask patient to relax the
fist, and then remove the tourniquet.
Note: The tourniquet must be released after a maximum
of two minutes* regardless of whether or not you have
completed the venipuncture task.
STEP 13
Gently withdraw the butterfly needle and give the patient
a clean gauze or dry cotton-wool ball to apply firm pressure
to the site to achieve hemostasis and avoid a hematoma.
Ensure that the patient has stopped bleeding, and then
apply tape and gauze to the venipuncture site.
STEP 14
Always use a safety transfer device for transferring blood
from a syringe to the vacuum tubes or the blood culture
bottles. [CLICK HERE] for the method of transferring
blood with a safety device.
If no safety transfer device is available place the vacuum
tubes in a test tube rack before inserting the needle into
the vacuum tube. Carefully penetrate the needle through
the tubes stopper and let the blood passively fill the tubes.
Warnings when transferring blood:
DO NOT hold the vacuum tube in your hand!
DO NOT exert pressure on the plunger of the syringe
to avoid hemolysis or causing the needle or stopper
to pop off, thus creating a spray of blood droplets
with the danger of exposing you and other people to
bloodborne pathogens.
STEP 15
If the tube used has additives, mix the contents by gently
inverting the tube 5-8 times (do not shake). Fill remaining
tubes and repeat the mixing routine after each tube has
been filled.
STEP 16
Discard sharps (e.g., the used needles and broken glass)
and syringe or blood-sampling device into a punctureresistant sharps container. Place other items like used
gloves and all items contaminated with blood or body
fluids into the infectious waste.
POINTS OF INTEREST:
Notes on safety:
Two examples of butterfly needles with built-in safety
features are:
The Punctur-Guard uses an internal blunt needle.
The mechanism is activated after blood is drawn.
The Angel Wing is activated by sliding a safety shield
over the needle after venipuncture.
Figure 29: The Angel Wing Safety butterfly needle [For more information]
Important note:
Always use a needle with safety features in a clinical
setting!
Safety needles minimize the risk of needle prick
injuries but do not eliminate these risks completely.
There is no substitute for being careful.
STEP 17
Recheck the labels and forms for accuracy.
STEP 18
Thank the patient and perform hand hygiene.
STEP 19
Simulate the project by following Steps 1-18 using the fully
set up Venipuncture Trainer. See PROJECT 10B SET UP
THE VENIPUNCTURE TRAINER FOR IV TRAINING.
Ensure that the tubes are filled with fake blood, the IV fluids
101
PROJECT 11 D
HOW TO START AN IV LINE
How to place a venous cannula into a vein
VIDEO
INFORMATION
Mastering the skill of setting up an IV line requires, time,
patience, and a lot of practice.
102
the tip of the needle and cannula are located in the vein
the needle is withdrawn and discarded and the cannula is
then advanced inside the vein and secured into position
with tape.
REQUIREMENTS
You will need:
The sharps container
Alcohol wipe
Clean gloves
Clean work surface cover
STEP 2
Assemble equipment and all the relevant items using
REQUIREMENTS above. Open the outer packaging of all
the items on the clean work surface cover.
Note: Use safety catheters and safety needles in all clinical
settings. When simulating the procedure, you may use a
regular catheter/needle but take great care not to prick
yourself with the sharp needle.
STEP 3
Perform hand hygiene.
See PROJECT 3A A TECHNIQUE FOR PROPER
HANDWASHING and PROJECT 3B CLEANING
HANDS WITH AN ANTISEPTIC RUB.
STEP 4
Connect the IV tubing to the Out port of the IV fluid bag.
Prime the line by pressing the chamber once or twice,
allowing it to fill about halfway. Open the control-flow
mechanism and allow fluid to fill the tube until all air
bubbles are out of the tube.
STEP 5
Select a suitable site for setting up an IV line. Start by
looking for a suitable vein on the dorsal part of the hand.
If no suitable vein is identified (or if you fail with the
venipuncture attempt) move proximally to the side of
the wrist, possibly the forearm, and then the cubital area.
Inspect and palpate the veins in the intended venipuncture
site. If necessary, you may temporarily apply a tourniquet
4-5 fingers above the intended venipuncture site to
facilitate inspection and palpation. Remove the tourniquet
until ready to proceed.
Place a linen saver under the patients arm to protect the
bed linens as it is difficult to avoid a couple of drops of
blood from occasionally flowing out of the vein.
Warning: When setting up an IV line, it is of utmost
importance to ensure that one is placing the cannula in a
vein, and not in an artery. When performing phlebotomy
procedures, an intra-arterial draw might not be the end
of the world, but as medication are often infused with an
IV line, an intra-arterial infusion of medication may have
catastrophic results!
103
STEP 6
STEP 12
STEP 7
When ready to perform the venipuncture, apply the
tourniquet about 45 fingers above the selected
venipuncture site.
STEP 8
Don preferably sterile gloves, otherwise use clean
examination gloves. See PROJECT 3C or 3H.
STEP 9
Ask the patient to form a fist to make the veins more
prominent. (Some clinicians will first ask the patient to
form a fist first, and then don gloves while the veins are
distending).
Important note: If you do not intend performing
phlebotomy before attaching the IV line, you may ask the
patient to clench and open the fist a couple of times to
facilitate vein distention.
STEP 10
Disinfect the site using 70% isopropyl alcohol for 30 seconds
and allow it to dry completely (another 30 seconds).
DO NOT touch the site again once the alcohol (or other
antiseptic) has been applied! If you must touch it again to
feel the vein, touch a clean alcohol pad first to aseptically
treat your glove.
STEP 11
Remove the protective cap from the needle section of
the catheter. Anchor the vein by holding the patients arm
and placing the thumb of the opposite hand below the
venipuncture site. Insert the needle, bevel up, at an angle
of 15-20. Some instructors advise students to imagine an
airplane landing on a runway as they insert the needle.
DO NOT insert the needle where veins are branching as
this will increase the chance of a hematoma.
104
STEP 13
Remove the tourniquet.
If required, take the opportunity to draw blood before
attaching the IV line.
STEP 14
Remove the plastic connector cap/plug from the catheters
connector (if applicable).
Apply finger pressure on the vein above the catheter to
prevent the retrograde flow of blood. Apply a small gauze
square under the catheter to catch any escaping blood.
STEP 15
Attach the IV tubing to the catheter. Secure the catheter
with tape and a transparent dressing.
STEP 16
Open up the IV line. If you were successful, you will see
fluid dripping in the drip-chamber of the tubing.
Observe the area for a couple of minutes. If the surrounding
tissue swells, the drip is infiltrating the tissue. Stop the drip,
apologize, and move to another site.
Important note: If you see a drop of IV fluid in the drip
chamber moving in and out in a pulsatile fashion then
you are probably in an artery. Stop the drip immediately,
remove the needle and apply pressure on this site for five
minutes.
To avoid grave complications, never inject medication into
an artery!
STEP 17
Adjust the drip rate to whatever is appropriate for your
patient. To keep the line open, turn the drip rate down to
just a few drops a minute.
STEP 18
Discard sharps (e.g., the used needles) into a punctureresistant sharps container. Place other items like used
gloves and all items contaminated with blood or body
fluids into the infectious waste container.
STEP 19
Thank your patient. Compliment pediatric patients for
being brave. Perform hand hygiene.
STEP 20
Simulate the project by following Steps 1-19 using
the Venipuncture Trainer. You will have to modify the
Venipuncture Trainer setup:
Do not attach the tubing to the tubes on the trainer. Place
a lumen stopper on one end of the tube/imitation vein
and a shallow container below the open end. When you
are convinced that you have successfully entered a vein
on the Venipuncture Trainer, attach the free end of the
IV tube to the catheter, and then open the flow-control
mechanism. If you were successful, fluid will flow out of the
open end of the fake vein. If no flow is observed, close the
flow-control mechanism and try again. Keep practicing!
POINTS OF INTEREST:
If you fail, a bit of blame shifting is quite permissible. Blame
it on the vein, on the weather or anything else, as you
need to retain your patients confidence in your abilities
for the next attempt! If you fail for a third time, apologize
and ask for assistance from a more experienced medical
professionalunless that person is you!
The veins of elderly people tend to slip to one or the other
side if you puncture it from the top. Secure the vein with
a finger of your other hand and puncture the skin on the
side of the targeted vein.
Example:
3000 ml IV Saline is ordered over 24 hours. Using a drop
factor of 15 drops/ml, how many drops per minute need
to be delivered?
3000 (ml) x 15 (drops/ml)
= 31.25 drops/minute
24 hrs. x 60 (gives us total minutes)
105
PROJECT 11 E
HOW TO REMOVE THE IV LINE
How to remove a venous cannula from a vein
VIDEO
ALTERNATIVES TO IV
INFUSION FOR ACCESSING
THE BLOODSTREAM:
Intraosseous infusion (commonly used in pediatric
patients)
106
SPECIAL GROUPS
OF PATIENTS
THE NEONATE PATIENT
Abstract:
Venous cannulation has been in regular use in neonates
since the 1940s. This was at first through the umbilical
vein, but the frequency of complications lead to other
central and peripheral routes being used for infusion of
fluid, nutrients and drugs. Today, peripheral venous access
is preferred except for high volume fluid resuscitation,
reliable infusion of irritant drugs and long-term parenteral
nutrition. Intraosseous infusion provides a reliable
alternative to peripheral veins for rapid infusion of fluid.
Long, thin silastic catheters can be inserted through a
peripheral venous cannulae for parenteral nutrition or
other central venous infusions as an alternative to direct
central venous cannulation using the Seldinger or other
techniques. Broviac or Hickman catheters, inserted through
a subcutaneous tunnel are only considered when central
venous cannulation is likely to be needed for more than six
weeks. The most common serious complication of vascular
access is infection. Infection associated with central
venous catheters is reduced by prophylactic vancomycin
or teicoplanin. Other complications of central venous
infusion are associated with cannulae malpositioning,
bleeding and thrombosis. Distal hypoperfusion may follow
arterial cannulation. Modern emergency and intensive
care paediatrics is impossible without adequate venous
and arterial vascular access; however, no other skill for
neonatal intensive care causes more anxiety among
primary care providers, is more difficult to teach and is
associated with an increased risk of median nerve injury.
For the complete article [Click Here]
107
VETERINARY
VENIPUNCTURE
The animal patient
The basic principles of performing venipuncture procedures on the animal patient are the same as for the human patient.
VIDEO
Generalized differences include:
Most animals do have fur or lots of hair covering the areas with veins suitable for venipuncture. Removal of fur or hair
is often necessary to visualize the veins and successfully perform phlebotomy or set up an IV line.
The temperaments of animals are quite varied and different from the human patient.
Communicating with animals is quite different to communicating with humans.
The general and thus venous anatomy of various animal species may vary considerably.
The skin of certain species of animals, for example reptiles, may be thick and difficult to penetrate.
When treating wild animals, levels of aggression may be dangerous or life threatening. A sedative (i.e., administered by
darting) may be a prerequisite before a successful venipuncture procedure may be safely performed.
Clinicians may need to take specialized protective measures to protect themselves from injury when treating animal
patients.
In veterinary practice, vascular access has many variables
and techniques specific to many different species that
may be encountered. This topic is beyond the scope of
this course. Veterinary students are referred to veterinary
literature and books for detailed information on
venipuncture in animals.
108
1. Vascular access: theory and techniques in the small animal emergency patient
Abstract
Acquisition of vascular access in the emergent small animal patient is one of the keys to successful management of
a population of patients that are often unstable with regard to their major body systems. Venous and intraosseus
cannulation allow for the administration of a variety of fluids and potentially life-saving medications. In addition, central
venous and arterial access also serves as conduits for atraumatic blood sampling and intravascular pressure monitoring.
A thorough knowledge of vascular access theory, the dynamics of fluid flow, vascular anatomy, catheter selection criteria,
and placement techniques are critical to the proper and safe use of the vascular access options that are available to small
animal clinicians today.
2. Vascular access techniques in the dog and cat
Abstract
The rapid and reliable attainment of vascular access may prove crucial for the provision of an effective therapeutic solution
in the critically ill or emergency small animal patient. Although in such cases it is more common to consider venous vascular
access for the administration of medication and for the measurement of venous pressures, the attainment of arterial
vascular access may prove just as important, allowing the direct measurement of arterial blood pressure and the sampling
of arterial blood. This article provides guidelines on appropriate catheter selection for vascular access, placement techniques
for both venous and arterial access, and procedures required for the long-term maintenance of these access sites.
Veterinary students will find The Apprentice Doctor Venipuncture Course and Kit a valuable resource for attaining the
basic venipuncture knowledge and skills required by their curriculum.
Here are a number of clinical examples of Venipuncture procedures in the animal patient: [SEE VIDEO CLIP ON PAGE 108]
AIDS
TO ASSIST THE CLINICIAN
VeinViewer
VeinViewer is a medical imaging device that uses nearinfrared light to produce a digital image of a patients veins
and project it directly on their skin.
See this YouTube video: [CLICK HERE]
AccuVein is a similar medical imaging device that uses a
specific frequency of light to produce a digital image of a
patients veins projected directly on their skin.
See: [CLICK HERE]
Breastlight
Breastlight was originally designed as a breast cancer
screening modality for examining the female breast for
nodules. However, it is also quite useful to show blood
vessels or venipuncture purposes!
The frequency of light waves penetrates soft tissue readily,
but shows blood vessels as dark lines.
For more information [CLICK HERE]
It is also more affordable than the previous two products.
Ultrasound
Ultrasound is useful in detecting problems with most of
the larger blood vessels in the body (e.g., the abdominal
aorta and the carotid arteries). Using Doppler ultrasound
technology, the flow of blood through vessels can be
observed and measured. This makes it possible to detect
arterial stenosis and aneurysms.
Ultrasound is also useful in demonstrating superficial or
deep veins and to differentiate between veins and arteries.
[CLICK HERE]
Anesthetists use ultrasound to guide them to correctly
place the needle when performing regional anesthesia.
Radiography
Central lines are commonly placed in critically ill patients. Chest
radiographs are used to ensure proper positioning and to rule
out complications during placement. The ideal location for the
tip of a central line is at the cavoatrial junction, which is where
the superior vena cava meets the right atrium. This allows for
the infusion of large volumes of fluids or medications.
Also see section on interventional radiology.
109
PROJECT 12A
IDENTIFY THE BODYS PULSE POINTS
How to identify the bodys pulse points and how to determine a persons heart rate
INFORMATION
HINTS
REQUIREMENTS
A suitable volunteer for checking the pulse points
A watch with a minute indicator (if you want to determine
the heart rate)
STEP 1
Look at this diagram showing a number of the more common pulse points of the body:
STEP 2
NOTE:
110
Note: A thorough knowledge of the regional anatomy will be helpful to locate these pulse points.
STEP 3
STEP 6
WARNING
Do not press too hard to find the carotid pulse in the neck.
Do not press on both carotid arteries at the same time. This
may cause fainting!
STEP7
Practice by identifying a variety of these pulse points and
checking the pulse rate of other people.
POINTS OF INTEREST:
STEP 4
Identify the brachial pulse an important pulse point used
for the purpose of taking routine blood pressure readings.
STEP 5
Identify the pulse points as shown in the diagram above.
The femoral pulse is reserved for self-examination.
111
PROJECT 12 B
PERFORM A MODIFIED ALLENS TEST
Check the collateral circulation of the hand
VIDEO
INFORMATION
In the majority of the population, two arteriesthe radial and ulnar arteriessupply the hand with oxygenated blood.
These arteries anastomose in the hand. In a minority number of people, this dual blood supply is absent.
The Allens test and the modified Allens tests are used to test the collateral blood supply to the hand, specifically the
patency (openness) of the radial and ulnar arteries. It is performed prior to radial arterial blood sampling or cannulation, as
well as before coronary bypass surgery as the cardiothoracic surgeon may choose to harvest the radial artery to be used
as a graft/conduit for bypass surgery.
112
INTERPRETATION:
When you release the occlusive pressure on the ulnar
artery, you should notice a return of normal color to the
palm and nails within 7 seconds. This indicates that the
ulnar artery is patent and has good blood flow.
Negative Allens test: The normal color (flushing) returns
within 7 seconds.
Positive Allens test: The normal color of the hand does
not return (flushing) within the specified time. A negative
modified Allens test indicates that ulnar circulation is
inadequate or nonexistent.
SIGNIFICANCE
Despite the fact that some researchers question the
validity of the Allens test, the following guidelines are still
recommended:
Negative: Allens test (normal pink color returns): You may
use the radial artery for blood sampling, cannulation, or
to harvest as a graft. The ulnar artery will be sufficient for
supplying blood to the hand, even without a patent radial
artery, should occlusion complications occur.
STEP 2
Elevate the hand and ask the patient to make a tight
clenching fist for about 30 seconds.
STEP 3
Apply firm pressure over the ulnar and the radial arteries,
occluding both of them.
STEP 4
Still elevated, the hand is then opened. It should appear
blanched. (Pallor can be observed over the palm, as well
as the fingernails).
STEP 5
Release the ulnar pressure and the color should return
within 7 seconds (between 5 and 15 seconds).
113
PROJECT 12 C
DRAW ARTERIAL BLOOD
Sample arterial blood from an artery
VIDEO
INFORMATION
REQUIREMENTS
114
STEP 2
Greet, identify, and inform the patient. Explain the
procedure shortly (unless comatose) and that they will
experience a small needle prick, a mosquito bite, or
whatever works for you. You can ask them to please, keep
your arm still.
STEP 3
Perform hand hygiene.
See PROJECT 3A A TECHNIQUE FOR PROPER
HANDWASHING and PROJECT 3B CLEANING HANDS
WITH AN ANTISEPTIC RUB.
STEP 4
Site selection: as a first choice, select the radial artery of the
non-dominant wrist. See PROJECT 12A IDENTIFY THE
BODYS PULSE POINTS.
Warning: Radial arteries are contraindicated in patients
who have a fistula or shunt in place for dialysis or have had
the radial artery used as a coronary artery bypass graft on
the side of the intended sampling.
STEP 5
Perform a modified Allens test. See PROJECT 12B
PERFORM A MODIFIED ALLENS TEST.
If you have a positive Allens test (normal pink color doesnt
return): dont use the radial artery for blood sampling or
cannulation so as to avoid serious ischemic (insufficient
blood supply) complications to the hand.
Rather use the radial artery of the opposite hand (remember
to do an Allens Test first) or choose another artery.
STEP 6
Position is important! The patient should be seated
comfortably (patients in bed should be in the semirecumbent position) and the arm must be comfortably
extended towards you, wrist up, and the skin over the
radial artery taut. Let the forearm rest on a small pillow;
use a rolled towel or linen under the back of the hand
to facilitate the extended wrist position. Cover the rolled
support with a linen saver.
STEP 7 (OPTIONAL)
Give local anesthetic. Don non-sterile gloves and prepare the
skin aseptically. Infiltrate (e.g., 2% plain Lidocaine 0.2 0.3 ml)
intradermally with a 25G needle to reduce the anticipated
pain associated with the procedure. Remove your gloves.
STEP 8
Perform hand hygiene, don sterile gloves, and disinfect
the site with an alcohol wipe for 30 seconds and allow to
dry completely (allow another 30 seconds).
STEP 9
With the fingertips of your gloved left hand, find the area
of maximal pulsation of the radial artery. In addition to the
pulsation, you should be able to feel the radial artery as a
cord-like structure beneath your fingers.
With the fingers of your left hand over the radial artery,
visualize the course of the radial artery underneath your
fingers in three dimensions.
STEP 10
Hold the syringe with the attached exposed needle in
your right hand like a pencil. Approach the skin at 3045, in line with the radial artery, pointing in the direction
towards the elbow. The needle should enter the radial
artery immediately below the gloved fingers of the left
hand (careful not to slip and injure yourself ). Keep the skin
taut and enter the skin with a brisk movement is the skin
penetration that causes the most pain.
STEP 11
Once in the soft tissue, slowly advance the needle to
where you think the radial artery is. Do not rush; it is easy
to go straight through the radial artery. You may feel a
slight give as the needle penetrates the wall of the radial
artery. Once the needle has entered the artery you should
see a flashback of blood pulsating into the syringe. If you
dont see blood you may have missed the artery or may
have gone right through it. If so, withdraw the needle until
blood starts filling the syringe or you may have to try again
by re-aiming the syringe towards the pulsating artery.
115
STEP 12
STEP 21
STEP 13
Immediately place a gauze pad or cotton ball over the
site and firmly apply pressure for 5-10 minutes. Use the
tourniquet over the cotton ball to apply pressure.
STEP 14
Inspect the syringe for air bubbles and slowly eject using
a gauze square. Mix the blood with the heparin by gently
rolling the syringe a couple of times between your fingers.
STEP 15
Seal the needle or tip of the syringe with a rubber stopper
to prevent the influx of air.
STEP 16
Place the syringe onto the ice (pack some ice cubes
over the syringe) and send it off to the lab immediately.
ABG samples should be analyzed within 10 minutes of
collection for accurate results.
STEP 17
Discard all sharps (e.g., the used needles, syringes) and
potential sharps such as glass items into a punctureresistant sharps container. Place other items like used
gloves and all items contaminated with blood or body
fluids into the infectious waste.
STEP 18
Recheck the labels and requisition forms for correctness of
the patients name, the date, time, puncture site, etc.
STEP 19
Monitor the site and extremity for a while for any
sign of circulatory problems, nerve damage, or other
complications.
STEP 20
Thank the patient and perform hand hygiene.
116
POINTS OF INTEREST:
Although arterial puncture is a fairly complicationfree procedure, you may encounter the following
complications:
Hematoma
Blood under pressure is initially more prone to leak from
an arterial puncture than from a venipuncture site. It is
important to exert sufficient pressure over the puncture
site for 5-10 minutes.
Hemorrhage
Hemorrhage is especially a problem with patients receiving
anticoagulant therapy or patients with blood coagulation
disorders. A longer compression time will be necessary.
Nerve damage
Compression neuropathy secondary to hematoma may
cause temporary numbness of the hand. Direct needle
injury to a nerve may cause permanent numbness of part
of the hand. Know your anatomy and avoid continuous
blind and deep poking of the wrist area.
Aneurysm and AV (Arterio-Venous) Fistula
These rare complications usually occur with repeated
punctures. An aneurism will cause the artery to balloonout due to a weakened muscular wall. An AV fistula is a
communication between an artery and a vein before the
capillary bed.
Arteriospasm
Arterial spasm may decrease the pulse volume and cause
pain but fortunately is temporary.
Thrombus formation
Injury to the artery can lead to clot (thrombus) formation. A
large thrombus can obstruct the flow of blood and impair
circulation to the hand.
Infection of the puncture site
Use sterile/aseptic protocol as recommended by your
institution/hospital.
BLOOD TRANSFUSIONS,
BLOOD TYPE (BLOOD GROUPS) AND AGGLUTINATION
A persons blood type is determined by certain proteins
markers (antigens) on the surface of red blood cells (RBCs).
A total number of 30 human blood group systems are now
recognized by the International Society of Blood Transfusion
(ISBT). There are many types of blood; however, the most
important ones are ABO and the Rhesus factor.
On average the general population has the following percentages of blood groups (percentages vary from country to country):
0+
A+
B+
AB+
0-
A-
B-
AB-
36%
28%
21%
5,0%
4%
4%
1,5%
0,5%
Table 7: Prevalence of Blood groups in the general population (approximate global averages)
117
AA or A0
BB or B0
AB
AB
00
118
AGGLUTINATION REACTION
(CLUMPING TEST)
Should a medical professional infuse the wrong blood
type to somebody by accident, the result will be that
blood will agglutinate (create clumps of red blood cells
followed by serious life threatening complications). It
is important to test the blood from the donor and the
recipient by mixing a small amount in a test tube or on a
glass slide to test for compatibility. Agglutination will be
noted if the bloods are incompatible. Type O Rh- blood
can be given to anybody because theres nothing on
the blood cells for the person to attack. A person with
type O Rh- blood is considered a Universal donor and
any person in any other blood group may receive type
O Rh- blood. A person with type AB Rh+ blood carries
both A, B, and D (Rh) antigens but neither A nor B nor
D antibodies and can therefore receive anyones blood
(types A or B or AB or O blood Rh+ or Rh-) because they
dont have antibodies to fight antigens. Type AB is known
as a universal recipient.
Recipient
00+
AA+
BB+
ABAB+
0-
0+
A-
A+
B-
B+
AB-
AB+
119
PROJECT 13
DONATING BLOOD FOR THE BLOOD BANK
Become a blood donor (if you arent a donor already)!
INFORMATION
Study pages 25 30 (Practical guidance on
venipuncture for blood donation) of the WHO
document:
WHO guidelines on drawing blood: Best practices in
phlebotomy. WHO Publication 2010
Your mission is as follows:
Identify your closest blood bank.
Arrange a date and time to donate blood.
Observe the steps and method used by the
phlebotomist to collect blood from you as a blood
donor and compare it with the information below.
Ask questions and have a hands-on learning
experience!
REQUIREMENTS
You will need:
The contact details of your local blood bank.
Transport to and from your local blood bank.
COLLECTING BLOOD
For collection of blood for donation use the procedure
detailed in Section 2 for blood sampling (e.g., for hand
hygiene and glove use) as far as it is relevant and follow
the six steps given below:
STEP 1
120
STEP 2
Select the vein
Select a large, firm vein, preferably in the cubital fossa,
from an area free from skin lesions or scars.
Apply a tourniquet or blood pressure cuff inflated to
4060 mm Hg to make the vein more prominent.
Ask the donor to open and close their hand a few
times.
Once the vein is selected, release the pressure device
or tourniquet before the skin site is prepared.
STEP 3
Disinfect the skin
If the site selected for venipuncture is visibly dirty,
wash the area with soap and water and then wipe it
dry with single-use towels.
One-step procedure (recommendedtakes about
one minute):
Use a product combining 2% chlorhexidine
gluconate in 70% isopropyl alcohol.
Cover the whole area and ensure that the skin
area is in contact with the disinfectant for at
least 30 seconds.
Allow the area to dry completely or for a
minimum of 30 seconds by the clock.
Two-step procedure (if chlorhexidine gluconate in 70%
isopropyl alcohol is not available, use the following
proceduretakes about two minutes):
Step 1: Use 70% isopropyl alcohol
Cover the whole area and ensure that the skin
area is in contact with the disinfectant for at
least 30 seconds.
Allow the area to dry completely (about 30
seconds).
Step 2: Use tincture of iodine (more effective
than povidone iodine) or chlorhexidine (2%).
Cover the whole area and ensure that the skin
area is in contact with the disinfectant for at
least 30 seconds.
Allow the area to dry completely (about 30
seconds).
Whichever procedure is used, DO NOT touch
the venipuncture site once the skin has been
disinfected.
STEP 4
Perform the venipuncture
Perform venipuncture using a smooth, clean entry
with the needle. Take into account the points given
below which are specific to blood donation.
In general, use a 16 gauge needle, which is usually
attached to the blood collection bag. A retractable
needle or safety needle with a needle cover is
preferred, if available, but all should be cut off at the
end of the procedure (as described in step 6 below)
rather than recapped.
Ask the donor to open and close their fist slowly every
10-12 seconds during collection.
Remove the tourniquet when the blood flow is
established or after 2 minutes, whichever comes first.
STEP 5
Monitor the donor and the donated unit
Closely monitor the donor and the injection site
throughout the donation process. Look for:
Sweating, pallor, or complaints of feeling faint
that may precede fainting
Development of a hematoma at the injection
site
Changes in blood flow that may indicate the
needle has moved in the vein and needs to be
repositioned
About every 30 seconds during the donation, mix the
collected blood gently with the anticoagulant, either
manually or by continuous mechanical mixing.
POINTS OF INTEREST
Current FDA guidelines allow a maximum of 10.5 ml/
kilogram body weight of whole blood to be collected every
eight weeks. The majority of blood collection facilities use
500 ml whole blood bags, with an additional 50 ml (10%)
allowed to be drawn for mandated screening tests.
Great advances have been made with minimizing the
need for blood during surgery. See Bloodless surgery for
more information: [Click Here]
STEP 6
Remove the needle and collect samples
Cut off the needle using a sterile pair of scissors.
Collect blood samples for laboratory testing.
121
CASE STUDY 4:
DESPITE ALL THE TRAINING AND THE NECESSARY CARE, ACCIDENTS DO HAPPEN
124
Warnings:
In all clinical settings use safety needles and dont
allow for any exceptions.
Ensure that your Hepatitis B inoculations are up to date.
125
Thrombosis
Other complications
Air embolism (rare)
Hemorrhage and formation of a hematoma
ARTERIAL
CATHETERIZATION
An arterial line is a thin, hollow tube that is inserted
into an artery the most common being the radial
or femoral arteries. It is often used in intensive care
medicine and anesthesia to monitor the blood pressure
real time and/or to obtain multiple samples for arterial
blood gas measurements. The arterial line must be
clearly marked to avoid accidental intra-arterial injection
of intravenous drugs.
126
RISKS INCLUDE
Pain: Discomfort can result from the needle stick and
placement of the catheter at the time it is inserted.
Consider infiltrating the skin over the intended
insertion site before catheterization.
Infection: As is the case with all catheters inserted
into the body, bacteria can travel up the catheter from
the skin and into bloodstream causing bacteremia or
septicemia. The longer the catheter remains in the
artery, the more likely it is to become infected.
Thrombus formation: If blood clots form on the tips
of arterial catheters, the clots may block blood flow
and, very rarely, may cause the loss of a hand or a
leg. This complication can be minimized by regularly
checking the flow of blood in the relevant extremity.
Bleeding: Bleeding may occur at the time of inserting
the catheter. Patients on anticoagulation therapy are
at high risk. The bleeding usually stops spontaneously,
but in some cases the catheter may require removal
followed by the application of pressure to the site.
CORONARY
ARTERIOGRAPHY
A cardiologist may catheterize the coronary arteries,
usually via a femoral artery access to evaluate the coronary
arteries or to perform an interventional procedure, such as
placing a stent.
VIDEO
INTERVENTIONAL
RADIOLOGY
Interventional radiologists utilize minimally invasive,
image-guided procedures to diagnose and treat diseases
in nearly every organ system. The concept behind
interventional radiology is to diagnose and treat patients
using the least invasive techniques available in order to
minimize risk to the patient and improve health outcomes.
Interventional radiologists pioneered modern minimally
invasive medicine using X-rays, CT, ultrasound, MRI, and
other imaging modalities. Interventional radiologists
obtain images which are used to direct interventional
instruments throughout the body. These procedures are
usually performed using needles and catheters instead of
making large incisions into the body as in conventional
surgery.
127
KIDNEY
DIALYSIS
Conventional chronic hemodialysis is usually done
three times per week for about 3-4 hours per dialysis
treatment, during which the patients blood is drawn
out through a tube at a rate of 200-400 ml/min. The
tube is connected to a 15, 16 or 17 gauge needle
inserted into the dialysis fistula or graft, or is connected
to one port of a dialysis catheter. The blood is pumped
through the dialyzer and then the processed blood is
pumped back into the patients bloodstream through
another tube connected to a second needle or port.
During the treatment, the patients entire blood volume
(about 5000 cc) circulates through the machine every
15 minutes.
128
KIDNEY
DIALYSIS
A port is most commonly inserted as a day surgery procedure in a hospital or clinic by a surgeon or an interventional
radiologist under conscious sedation.
Implantable ports are often used to give chemotherapy treatment and/or other medicines to cancer patients.
Chemotherapy is relatively toxic to normal cells and can damage skin and muscle tissue, as well as small veins.
129
PARENTERAL NUTRITION
(TPN)
Parenteral nutrition is given intravenously.
Partial parenteral nutrition supplies only part of daily
nutritional requirements, supplementing oral intake.
Many hospitalized patients are given dextrose or amino
acid solutions by this method.
ANESTHESIA
LOCAL ANESTHESIA
A local anesthetic is a drug that causes reversible local
anesthesia (loss of sensation), inducing the blocking of
pain impulses to the brain with the aim of performing a
pain-free procedure.
Topical anesthetics are usually in the form of a
cream, gel, or spray and are applied to the skin or
mucous membrane before penetrating it with a needle.
Applications include ophthalmology, dentistry, the relief
of symptoms (e.g., sun burn), and before venipuncture.
130
PROJECT 14
INFILTRATING A WOUND WITH LOCAL
ANESTHETIC BEFORE SUTURING
Two methods of infiltrating a wound with a local anesthetic solution before debridement and suturing
VIDEO
INFORMATION
REQUIREMENTS
131
METHOD 1
FOLLOW THESE STEPS AS
IF IN A REAL-LIFE CLINICAL
SITUATION:
STEP 1
Remember the basics: take a medical history, prepare your
hands hygienically, and don clean gloves.
STEP 2
Withdraw some of the fluid from one of the IV fluid
bags from the out port. This will be used as a fake local
anesthetic solution.
STEP 3
Draw a 5 cm (2 inch) line on the Venipuncture Trainer to
represent the laceration.
STEP 4
Insert the needle into the tissue, about 5 mm (1/4 inch)
away from the laceration, beside one end of the laceration.
STEP 5
Deposit a drop or two of local anesthetic and wait for 3040 seconds. Advance the needle parallel to the long axis of
the laceration to the other end of the laceration or to the
length of the needle.
STEP 6
Aspirate to ensure that you are not in a blood vessel, to
avoid accidentally injecting the local anesthetic solution
intravenously.
STEP 7
Inject the local anesthetic solution continuously just below
the skin as you withdraw the needle. Note the skin rising
as you deposit the local anesthetic fluid. With a real patient
you will see blanching (the tissue will become whiter).
STEP 8
If the laceration is longer than the needle, repeat the
same procedure along the next section of skin next to the
laceration but ensure that you enter the skin in already
locally anesthetized skin.
132
STEP 9
Repeat the same procedure on the other side of the
laceration.
STEP 10
Wait 2-3 minutes; test the effectiveness of the local
anesthetic by poking the wound area with a probe or
pinching it with a forceps. You are now ready to prepare
the wound for suturing.
Information:
Do you want to learn all the basics about suturing, such as
how to tie a surgeons knot and learn 12 different suturing
techniques? Get your very own Apprentice Doctor How
to Suture Wounds Course and Kit
STEP 11
Lift the skin, dry the trainer, and discard used needles in
the mini sharps waste container. Clean up and replace all
reusable items in your kit. Keep in a safe place and out of
reach of children!
METHOD 2
For this method, use a thin 27G needle. A short dental syringe and needle will work just fine.
Penetrate the laceration through the raw edge of the wound. Starting at one side of the laceration, advance the needle
into the adjacent tissue for about 1 cm (3/8 inch) at an angle of about 30-45. Repeat the same process of injecting local
anesthetic solution every 7 mm (1/4 inch) on both sides of the wound. Follow with Step 9 as above.
By avoiding the penetration of intact skin, the patient experiences significantly less pain compared to Method 1, although
most people would think that injecting straight into the wound should be more painful.
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POINTS
OF INTEREST
Local and general adverse effects and complications
include:
GENERAL SYSTEMIC
ADVERSE EFFECTS
Central nervous system
Dangerous side effects involving the central nervous
system usually follow when the safety dosage margins
of the specific drug were exceeded or when an
inadvertent intravenous or intra-arterial injection of the
local anesthetic has occurred. It may either excite or
depress the central nervous system which may manifest
at lower blood levels as convulsions or coma, respiratory
arrest, and death at higher concentrations
Cardiovascular system
Complications related to the conductive system of the
heart include heart palpitations (innocent and usually
due to the vasoconstrictors effects), arrhythmias, and
a complete heart block (extremely rare but potentially
fatal if not treated promptly)
Allergic reactions
An allergy may vary from hypersensitivity (e.g., skin rash
and itchiness) to a life-threatening anaphylactic shock.
A patient may be allergic to any one of the components
in a local anesthetic solution:
The local anesthetic (The two main groups
are esters and amides.)
The vasoconstrictor
The preservative
Ask specifically about allergies to local anesthetics when
preparing for suturing under local anesthetic.
Be prepared with all the emergency equipment to
resuscitate a patient in the case of an allergic reaction.
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General anesthesia
Prerequisite before starting the administration of a
general anesthetic is a dependable venous access
route for injecting the induction agent, various other
medications, as well as possible emergency medications.
Total Parenteral Anesthesia (TPA) is the term used when
the anesthetist administers a general anesthetic using
intravenous drugs, which are infused with an infusion
pump, instead of maintaining the anesthetic with
volatile anesthetic gasses.
Infusion pumps
External infusion pumps are medical devices that
deliver fluids, including nutrients and medications (such
as anesthetic agents, antibiotics, chemotherapy drugs,
and pain relievers) into a patients body in controlled
amounts. Many types of pumps, including large volume,
patient-controlled analgesia (PCA), elastomeric, syringe,
enteral, and insulin pumps, are used worldwide in
healthcare facilities such as hospitals and in the home.
NEW
DEVELOPMENTS
Scientists and bioengineers are developing amazing
new technologies. Here are a few examples:
Microprobes for continuous monitoring
Instead of frequently sampling blood and then sending
it to the lab and waiting for results, certain biochemical
substances can be monitored very accurately and in
real time using an indwelling microprobe. This has
especially useful applications in diabetics with real time
monitoring of blood glucose.
[CLICK HERE] for more information
Needleless injections
In future, people who have a fear of needles one may
barely perceive receiving a needleless injectionat
most they may feel the discomfort of a nail-scratch!
How it works:
Commercially available needleless injection systems:
Have a look at this pain-free, needleless dental injection:
[CLICK HERE]
And other pain-free injections: [CLICK HERE]
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SHORT NOTES
ON OTHER BODILY SECRETIONS
SALIVA
BREAST MILK
SPUTUM
Sputum refers more to the mucous substance secreted
by the mucous glands in the throat and upper airways
(nasal mucosa, trachea, bronchi, and bronchiole).
Sputum needs to be coughed up and spit out by the
patient into a specimen collection container. Sputum
is usually sent to the microbiology lab and is especially
useful in the diagnosis of tuberculosis (deep early
morning sputum collected three consecutive days).
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SEMEN
Basic semen analysis: Almost all laboratories will report
on the following information using values established
by the World Health Organization.
SWEAT
The sweat chloride test (sweat test) measures the
concentration of chloride that is excreted in sweat. It is
used to screen for cystic fibrosis (CF).
Sweating is stimulated by applying a colorless, odorless
chemical that causes sweating. An electrode is applied
SHORT NOTES
ON OTHER BODILY EXCRETIONS
URINE
FECES
URINALYSIS
The urine can be tested very quickly using a strip of special
paper, which is dipped in urine just after urination. This will
show if there are any abnormal products in the urine such
as sugar, protein, or blood.
If more tests are needed to get more details, the urine will
be analyzed at a laboratory.
Normally urine is sterile, but skin contaminant bacteria
may be added to the sample during urination.
The patient should be instructed to wash genital areas
before taking the sample, specifically taking the midstream urine.
A urine sample for the lab is collected in a standard lab
collection container (100-150 ml required).
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SHORT NOTES
ON OTHER BODILY FLUIDS
Cerebrospinal fluid (CSF) is a clear, colorless bodily fluid
produced in the choroid plexus of the brain and occupies
the subarachnoid space, the ventricular system around
and inside the brain, as well as the central canal of the
spinal cord.
CSF can be tested for the diagnosis of a variety of
neurological diseases. It is commonly obtained by a
procedure called a lumbar puncture. Lumbar puncture
is performed in an attempt to count the cells in the fluid
and to detect the levels of biochemical constituents like
protein and glucose. These parameters alone may be
extremely beneficial in the diagnosis of subarachnoid
hemorrhage and central nervous system infections such
as encephalitis and meningitis. Microbiological CSF culture
examination may yield the specific microorganism causing
the infection.
By using more sophisticated methods, such as the
detection of the oligoclonal bands, conditions like multiple
sclerosis may be recognized.
Beta-2 transferrin is almost exclusively found in the
cerebrospinal fluid. It is not found in blood, mucus, or tears,
thus making it a specific marker of cerebrospinal fluid and
the detection of leakage like CSF rhinorrhea.
Figure 44: Cerebrospinal fluid fills the ventricles and surrounds the brain
and spinal cord.
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JOINT EFFUSION is the presence of increased intraarticular fluid, a fairly common finding in the inflamed
knee joint, but any joint may be affected. It may happen as
a result of trauma, inflammation, hematologic conditions
or infections.
EXUDATES AND
TRANSUDATES
AN EXUDATE is any fluid that filters from the circulatory
system into lesions or areas of inflammation.
It is rich in the protein and cellular elements that ooze out
of blood vessels due to inflammation and is deposited
in surrounding tissues. The altered permeability of blood
vessels permits the passage of large molecules and cells
through the blood vessel walls.
A TRANSUDATE is an accumulation of fluid that passed
through a membrane due to increased pressure in the veins
and capillaries forcing the fluid through the vessel walls.
This process filters out most of the protein and cellular
elements, thus yielding a watery solution. Transudates are
caused by disturbances of hydrostatic or colloid osmotic
pressure and not by inflammation.
There is an important distinction between transudates and
exudates. Exudates have a higher protein content and thus
a higher specific gravity, while transudates have a lower
protein content and thus a lower specific gravity.
PUS
Pus is a viscous, yellowish-white fluid formed in infected
tissue, consisting of white blood cells, cellular debris,
necrotic tissue and masses of bacteria, both dead and alive.
The following are recommended steps to take for
sending a pus sample from a closed abscess to the
microbiology lab for MC&S (Microscopy, Culture and
Sensitivity).
COMMENT:
Pus aspirated in a syringe is always preferable to a
swab.
Sample pus, if possible, before initiating antibiotic
therapy.
Contaminant bacteria (normal resident bacteria),
such as Staphylococcus epidermidis (skin) and
Streptococcus viridians (mouth and throat), grow
easily and often overgrow the pathological bacteria in
the lab giving valueless results.
The empirical treatment of an abscess is incision and
drainage.
External heat therapy increases the blood flow to the
area and assists with localizing the pus.
Antibiotics and analgesics play a supportive role in
treating infections. Not even the strongest antibiotic
will clear a pus-producing abscess!
As far as possible, use a narrow spectrum antibiotic
with proven sensitivity rather than treating the
infection blindly.
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CASE STUDY 5:
A ROUTINE VENIPUNCTURE CASE
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Important to remember:
Always take a short medical history (or check the
medical history in the patients records) before
performing venipuncture or setting up an IV line.
Use the veins of the foot as a last resort, especially if
any contraindications are noted.
VASOVAGAL RESPONSE
AND VASOVAGAL SYNCOPE
A vasovagal attack is a disorder that causes a rapid drop
in blood pressure and heart rate, resulting in decreased
blood flow to the brain, followed by fainting. It is most
often evoked by emotional stress associated with fear or
pain. The clinician will notice the following signs: pallor,
nausea, sweating, bradycardia, a rapid fall in arterial blood
pressure, and eventually, loss of consciousness. Symptoms
include lightheadedness, nausea, the feeling of being
extremely hot (accompanied by sweating), ringing in the
ears (tinnitus), an uncomfortable feeling in the heart, and
incoherent thoughts.
It is not too uncommon to see a vasovagal attack or syncope
(fainting) during or following venipuncture.
Not drive home (and not drive at all) on the day blood
is drawn.
Clinicians should:
Anticipate the possibility of a vasovagal syncope
and prevent injury to the patient by assisting and
supporting the patient.
Reassure the patient frequently.
Not show off their equipment in front of the patient,
especially sharp needles.
Have calming music in the background.
Have the patient comfortably seated, or if prone to
vasovagal attacks, in the supine position.
Have simple monitor equipment available: a blood
pressure meter and a basic pulse Oximeter.
When reporting for venipuncture or donating blood,
patients should:
Have a light meal before the procedure (unless they
have specific instructions regarding fasting).
Sit down comfortably for a couple of minutes after the
procedure (under supervision) before leaving.
Have a light refreshment after the procedure, especially
after donating blood.
Leave with a responsible person, instructed to support
the patient and what to do if syncope occurs.
Consider asking for a wheelchair instead of walking to
their transport.
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ALLERGIC
RESPONSES
A number of different allergic reactions may be
encountered during routine venipuncture procedures,
including the following:
CONTACT DERMATITIS
This usually manifests as an increased redness of the
skin where a specific strapping was placed.
Remove strapping
Apply a suitable cortisone-containing ointment
and give instructions on further use.
Inform the patient regarding the specific brand
of strapping. Ask them to avoid it in future and to
inform medical professionals when necessary.
SKIN RASH/
urticaria following the infusion of medication or
administration of a local anesthetic
Stop the infusion immediately and inform the
medical professional in charge of the patient.
Treatment with IV or oral antihistamines and/or
cortisone will usually suffice as definitive treatment.
Inform the patient regarding the specific
medication so that they can avoid it and inform
medical professionals accordingly in the future.
The patient should arrange for a Medical Alert
bracelet engraved with relevant information.
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CAUSES
Any medication may potentially trigger anaphylaxis.
Other causes include severe latex allergy and food
allergens.
DIAGNOSIS
Anaphylaxis is diagnosed based on clinical criteria.
When two or more of the following signs occurs
within minutes or hours of exposure to an allergen,
there is a high likelihood of anaphylaxis:
a. Involvement of the skin or mucosal tissue
(an itchy rash and/or urticaria)
b. Respiratory difficulty
c. Low blood pressure
d. Gastrointestinal symptoms
TREATMENT
Anaphylaxis is a medical emergency that may require
resuscitation measures such as airway management,
supplemental oxygen, large volumes of intravenous
fluids, and close monitoring.
NEEDLE PENETRATION
THROUGH THE VEIN
Withdraw the needle somewhat, re-angulate the needle
a bit more superficially, and enter the vein lumen. If
unsuccessful, apply pressure and move to another site. As
a general rule, if performing phlebotomy (drawing blood
for the lab), move to a more distal site from the previous
attempt (not proximal), or move to another extremity. If
putting up an IV line, change to a more proximal site from
the previous attempt (not distally).
HEMATOMA
If a hematoma forms under the skin adjacent to the
puncture site release the tourniquet immediately and
apply firm pressure while withdrawing the needle. Move
to another site. Older patients are prone towards forming
hematomas.
Considerations for preventing a hematoma:
Use the major superficial veins.
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ECCHYMOSIS
Even in the best of hands mild bruising may occur
occasionally, especially so in very fair skinned patients and
older patients.
Management:
For mild bruising, a simple explanation to the patient
will usually be sufficient.
For more severe bruising, apply a cold pack with
pressure to help limit bruising.
A physical therapist can apply ultrasound to help
break down a blood clot and diffuse ecchymosis.
Figure 47: Extensive ecchymosis in an elderly patient
NEEDLE/
CANNULA IN THE TISSUE
If performing phlebotomy and you land up in the tissue surrounding the vein, you have one of the following choices:
Go a bit deeper, if you are right above the vein.
Go laterally towards the vein, if you are on the side of a vein.
If you saw a flashback of blood and it disappears, you may have to withdraw the needle a bit as you may have
gone right through the vein.
If no luck, move to another site or ask a more experienced colleague to assist.
TISSUE INFILTRATION
(EXTRAVASATION)
If the needle lands up in the tissue during IV infusion
of fluid/medication, the IV fluid will infiltrate into the
surrounding tissue. The tissue will swell around the IV
needle, becoming edematous and cool to the touch.
The patient will complain about pain and discomfort.
Stop the infusion immediately!
Start the IV in a new spot on the patients body at
the correct rate for the given dosage.
Observe the infiltrated area for 24 hours for
possible complications. Treat these complications
empirically.
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CANNULA/
CATHETER BLOCKED (OCCLUDED)
CATHETER-RELATED
INFECTIONS
Central venous catheter-related infections are common
and an estimated 80,000 central venous catheter
related bloodstream infections occur in intensive care
units each year. Students should study the following
CDC publication for detailed information including
prevention guidelines. [CLICK HERE]
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CLINICAL DIFFERENTIATION
BETWEEN ARTERIES AND VEINS
WARNINGS:
NEVER inject any medication or fluids into a blood vessel
unless you are 100% sure you are inside a VEIN!
When setting up an IV line and if you suspect that the
needle has entered an artery stop the procedure, remove
the needle, and apply firm pressure as instructed above.
SUPERFICIAL
PHLEBITIS
Superficial phlebitis, also called superficial throm
bophlebitis, is a condition where a vein close to the surface
of the body becomes tender, swollen, red, and develops
a blood clot. This is differentiated from thrombophlebitis
of the deep veins of a limb (usually a lower limb), which
is called deep vein thrombosis or DVT.
Sometimes phlebitis may occur at the site where
a peripheral intravenous (IV) line was started. The
surrounding area may be sore and tender along the vein.
Thrombophlebitis may be caused by damage to a veins
wall as a result of injecting substances that cause irritation
or introduce bacteria into the vein from a contaminated
needle/cannula as well as the prolonged insertion of
a cannula for intravenous infusion.
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SEPTIC THROMBUS
If a thrombus becomes septic, or invaded by pathogenic bacteria, the patient will become febrile with all the local
and systemic signs and symptoms of infection (raised white blood cell count, etc.). In severe cases, septic shock may
ensue. Treat empirically with relevant antibiotic therapy and supportive treatment. Blood cultures may be required.
DEEP VEIN
THROMBOSIS (DVT)
It is not recommended to use veins, for either
phlebotomy or setting up an IV line, in the lower
extremities of adults due to possible complications that
include, but are not limited to, phlebitis and/or DVTs
especially in high risk individuals:
Patients with a history of thrombosis
Patients who will be immobile for an extended
period of time (e.g., orthopedic traction patients)
The elderly
Diabetics
People with blood disorders
Women who take oral contraceptives (birth control
pills)
People who have just undergone major surgeries or
have just suffered a bone fracture
Signs and symptoms of deep vein thrombosis
include:
Tenderness in the calf
Leg tenderness
Pain in the leg
Swelling of the leg
A warmer than normal leg
Redness in the leg
Bluish skin discoloration
Discomfort when the foot is flexed
Treatment of DVT includes:
Bed rest. Individuals with DVT usually require bed
rest until symptoms are relieved. The leg should be
elevated to a position above the heart to reduce
swelling. Moist heat may be applied to the affected
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EMBOLISM
Lung embolism from a dislodged deep vein thrombus
is a life-threatening condition requiring treatment in an
intensive care environment by suitable qualified and
experienced specialists in this field.
AIR
EMBOLISM
An air embolism is caused by air bubbles in the vascular
system. Venous air embolism can result from the
introduction of air through intravenous lines, especially
central lines, and generally must be substantial to block
pulmonary blood flow and cause symptoms.
Small amounts of air often get into the blood circulation
accidentally during surgery and other medical
procedures, but most of these air emboli enter the
veins and are stopped at the lungs. Thus, a venous air
embolism that shows any symptoms is very rare.
The risk of catheter-related venous air embolism is
increased by a number of factors:
Breakage or detachment of catheter connections
Failure to occlude the needle hub/catheter during
insertion or removal
Dysfunction of self-sealing valves in plastic
introducer sheaths
Presence of a persistent catheter tract following the
removal of a central venous catheter
Deep inspiration (inhalation) during insertion
or removal, which increases the magnitude of
Treatment:
The primary aim is to identify the reason for air entry
and prevent further air embolization.
Supportive care includes the use of mechanical
ventilation, vasopressors, and volume restoration.
The following may be of value:
High-flow supplemental oxygen
Hyperbaric therapy
Placing the patient in the Trendelenburg position
and other positional maneuvers may help in
dislodging the air embolus
Closed-chest cardiac massage
Aspiration of air from the venous circulation
With air embolism, prevention is better than cure!
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NEEDLESTICK INJURIES
Needlestick injuries may involve the patient or the
medical professional.
There are very specific legalities to be aware of, ways to
minimize you and your patients risk factors and steps to
take if such an incident should occur.
Kindly familiarize yourself with the most current
information on the subject.
Study the legalities: (USA) [OSHA Occupational
Safety & Health Administration]
Medical professionals outside of the USA, contact your
countrys Health and Safety authorities for information
regarding legalities and other specifics.
For sensible guidelines and more information see:
[Click Here]
VIDEO
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LOCAL
TISSUE DAMAGE
The skin surrounding the venipuncture site may (rarely)
break down, usually due to infection.
Applying a local antiseptic or antibiotic ointment may be
all that is required.
NERVE DAMAGE
The two nerves with the highest risk of being injured
during a venipuncture procedure are the radial and
median nerves. Permanent nerve damage is a difficult
complication for the patient to come to grips with and
carries a high medicolegal risk.
occur.
Recognize the signs and symptoms of nicking a nerve
and take appropriate action.
If your patient complains of an electric shock sensation
radiating down into the hand while the needle is
being inserted, remove the needle immediately to
minimize nerve injury.
Danger areas:
The distal part of the radial nerve just above the
thumb (radial nerve)
The inner/medial cubital fossa (median nerve)
The inner aspect of the wrist above the palm of the
hand (median nerve)
Although it is considered safe to use the cephalic vein in the
lateral aspect of the antecubital fossa area, risk of damage
has occasionally been described to the lateral antebrachial
cutaneous nerve of the arm following phlebotomy.
Arterial cannulation
Brachial artery cannulation is associated with an increased
risk of median nerve injury.
153
ASSESSMENT
MODULE
(Available online) [CLICK HERE]
The evaluation module consists of two sections:
Section 1. Short multiple choice questions (20 marks)
Section2. A selection of one practical project performed
by the student and assessed by a designated evaluator
(20 marks)
CONGRATULATIONS
AND FINAL REMINDERS
Congratulations you have completed The Apprentice
Doctor Venipuncture Course!
May we remind you carefully close the sharps waste
container and to hand it to a medical professional at a
hospital, a medical clinic, or to your family doctor for
proper sharps waste disposal.
Warning:
Never discard the sharps into a regular waste bin or bag!
EPILOGUE
In essence, the simple technique of venipuncture is a minor surgical procedure and all the rules common to surgery
apply. On occasion, simple procedures may become complicated due to various reasons. The most serious complication
following a simple venipuncture procedure is deathusually as a complication of a complication.
Be alert and minimize the risks to your patients for developing complications and your risk regarding medicolegal
consequences.
REFERENCES
1. Mario Saia,et al. Needlestick Injuries: Incidence and Cost in the United States, United Kingdom, Germany, France,
Italy, and Spain. Biomedicine International 2010; 1: 41-49.
2. Preventing Needle-stick Injuries in Health Care Settings. CDC Publication 1999.
3. Sharps Injuries: Assessing the burden of disease from sharps injuries to health care workers at national and
local levels. WHO Publication 2005.
4. WHO Guidelines on Hand Hygiene in Health Care. WHO Publication 2009.
5. Kouji Yamada et al. Cubital Fossa Venipuncture Sites Based on Anatomical Variations and Relationships of
Cutaneous Veins and Nerves. Clinical Anatomy. 2008 21: 307313.
6. Joan Barenfanger et al. Comparison of Chlorhexidine and Tincture of Iodine for Skin Antisepsis in Preparation for Blood
Sample Collection. J Clin Microbiol. 2004 May; 42(5): 22162217.
156
7. WHO guidelines on drawing blood: best practices in phlebotomy. WHO Publication 2010.
8. Mller JC, Reiss I, Schaible T. Vascular access in neonates and infantsindications, routes, techniques and devices,
complications. Intensive Care World. 1995 Jun; 12(2):48-53
9. Laura L et al. Difficult Venous Access in Children: Taking Control. JOURNAL OF EMERGENCY NURSING September
2009; 35:5
10. Beal MW, Hughes D. Vascular access: Theory and techniques in the small animal emergency patient. Clin Tech
Small Anim Pract. 2000 May; 15(2): 101-9.
11. Rob White. Vascular access techniques in the dog and cat. In Practice 2002; 24: 174-192.
12. Guidelines for the Prevention of Intravascular Catheter-Related Infections. CDC Publication 2011.
CREDITS
Facilities for videos and photography:
Rhesa Van Der Merwe: Hospital Manager, Union
and Clinton Hospitals
Hans Van De Zee: Specialist Veterinary Surgeon,
Valley Farm Animal Hospital in Pretoria
Our gratitude to all the skillful veterinary practitioners and
staff at Valley Farm Animal Hospital.
[CLICK HERE] to meet the team.
Patient models:
Anton Scheepers
Elna Van Der Hever
Jacquiline Sumerville
Rgardt Scheepers
Ruan Klut
Stfan Scheepers
Graphic design:
Maria Andor
Package, DVD, EBook, Brochure, and various other
graphic design aspects.
Portfolio site:
http://www.behance.net/marcsiandor
Production:
Open Window School of Visual Communication Hub
Arthur Twigge (Coordinator)
Chase Jordan Coetzee (Assistant videographer)
Dagan Read (Software development and final
compiling)
Natalie Scheepers (Photography - Dip Visual
Communication)
Stephan Calitz (Games and other interactive
components)
Wihann Strauss (Videographer and editing of
videos)
Illustrations:
Kevin Berry: Medical and General Illustrator
Drawing Conclusions:
www.drawingconcusions.co.za
Professional RNs:
Adelle Du Toit, RPN
Annette Klut, RPN
Lili Van Der Zee, RPN
Linguistic care:
Eizabeth Scheepers
Jacqui Summerville
Natalie Scheepers
Voiceovers:
Female:
Suehyla El-Attar
Voice123.com
Male:
Craig Gildner
Voice123.com
Stories (narration):
Dave Pettitt
Voice123.com
Model:
Gizela Marais
Box/package cover and DVD
Email: Giz.lubbe@gmail.com
157
OTHER PRODUCTS
BY THE APPRENTICE CORPORATION
After completing The Apprentice Doctor Venipuncture course, would you like to be able to confidently tie surgical knots
and suture wounds?
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GLOSSARY
KINDLY NOTE:
The Apprentice Doctor Venipuncture Course glossary does not include most of the common anatomical nomenclature
(terminology). Students are referred to their anatomy resources for definitions of those terms.
ABG Arterial Blood Gas.
ABO blood group The major human blood type system
which depends on the presence or absence of antigens A
and B.
Absorb To suck up or take in, as through pores.
Acid-citrate-dextrose (ACD) An anticoagulant containing
citric acid, sodium citrate, and dextrose.
Acquired immunodeficiency syndrome (AIDS) A disease
caused by an infection of the human immunodeficiency
virus (HIV-1, HIV-2).
Acute Of short duration. Rapid and abbreviated in onset in
reference to a disease process.
Adsorb To attract and retain other material on the surface.
Adult Respiratory Distress Syndrome (ARDS) A life
threatening inflammatory reaction of the lungs in response
to various forms of injuries or acute infection.
Aerobic Referring to organisms requiring an oxygenated
environment to grow and live.
Agglutination The process of cells clumping together,
such as red blood cells or bacteria, with the formation of
clumps of cells.
AHF Antihemophilic Factor. See Factor VIII.
AIDS Acquired Immune Deficiency Syndrome, caused by
human immunodeficiency virus (HIV).
Air Embolism (Emboli) An air embolism is a potentially
fatal pathological condition caused by air bubble/s in a
blood vessel and/or one or more of the heart chambers.
Albumin Main protein in human blood.
Allens test (Modified Allens test) Allens test is used to
test blood supply to the hand, specifically, the patency of
the radial and ulnar arteries.
Allergen A substance capable of producing a
hypersensitivity reaction (allergy).
Allergy An unusual sensitivity to a normally harmless
substance that provokes a strong reaction in a persons
body.
Ambulatory Mobile, walking around.
Anaerobic Organisms that can grow, live, and multiply in
the absence of oxygen.
Anaphylaxis (Anaphylactic Shock) A serious allergic
reaction that is rapid in onset and may cause death. It
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