Code No: RR322304

Set No. 1

III B.Tech II Semester Supplementary Examinations, Aug/Sep 2006 BIOPROCESS ENGINEERING-II (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks 1. How can you classify the bioreactors based on the operational mode and explain about them ? [16] 2. Write short notes on: (a) One-parameter model (b) Two-parameter model 3. Explain about Stability analysis of bioreactors? 4. How can you determine (a) Cellular ATP content (b) Single cell characteristics (c) Assay of cells with and without plasmids. 5. Discuss in detail about computer interfaces and peripheral devices. 6. Explain in detail about the various stages in gene expression. 7. Explain the integration of major metabolic pathways in an animal cell. 8. Discuss in detail about growth cycle phases for batch cultivation. [16] [16] [16] [16] [16] [6+4+6] [8+8]

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Code No: RR322304

Set No. 2

III B.Tech II Semester Supplementary Examinations, Aug/Sep 2006 BIOPROCESS ENGINEERING-II (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks 1. What are the dierent types of the bioreactors employed in Animal Cell Culture ? Explain about packed glass bead reactors and Perfusion cultures ? [16] 2. Write short notes on: (a) Filter sterilization of liquids (b) Continuous sterilization (c) Sterilization of gases. 3. Name the type of bioreactor in which heterogeneous biocatalyst particles are suspended by drag forces exerted by rising liquid and explain in detail about it with a neat diagram? [16] 4. Outline the procedures involved in the quantication of total RNA content and total Protein content in microbial cells ? [16] 5. Discuss in detail about gas analysis system based on a microcomputer with schematic diagram. [16] 6. Explain in detail about the various stages in gene expression. 7. Discuss in detail about product formation kinetics. 8. Discuss in detail about growth cycle phases for batch cultivation. [16] [16] [16] [6+4+6]

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Code No: RR322304

Set No. 3

III B.Tech II Semester Supplementary Examinations, Aug/Sep 2006 BIOPROCESS ENGINEERING-II (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks 1. Explain the basis for classication of Bioreactors and write in brief about them with an example? [16] 2. Write short notes on: (a) Spiral heat exchanger (b) Flash cooler sterilizer. 3. Explain the design and operation of Fed-batch reactor with mass-balances? [16] [8+8]

4. Write in detail about the methods employed in measuring the following process variables. [8+8] (a) Temperature (b) Pressure. 5. Explain the design and operating strategies for batch plants. 6. Explain the guide lines for choosing Host-Vector system. 7. Discuss in detail the scale-up of animal cell cultivation using BHK. 8. Discuss in detail about growth cycle phases for batch cultivation. [16] [16] [16] [16]

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Code No: RR322304

Set No. 4

III B.Tech II Semester Supplementary Examinations, Aug/Sep 2006 BIOPROCESS ENGINEERING-II (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks 1. List out the dierent accessories of a bioreactor and their uses ? 2. Write short notes on: (a) Non-Ideal Tubular reactor (b) Non-Ideal CSTR. 3. Explain about Stability analysis of bioreactors? [16] [16] [16]

4. What is Flow injection instrumentational analysis? Explain its role in bioprocessing? [16] 5. (a) Discuss in detail about ON-Line Sensor. (b) Discuss in detail about OFF-Line Analytical Methods 6. Explain in brief dierent types of products of Genetic Engineering. 7. Write Short Notes On: (a) Monoclonal antibodies. (b) Immunobiological Regulators. (c) Virus Vaccines. (d) Hormones. 8. Discuss in detail about growth cycle phases for batch cultivation. [16] [8+8] [16] [16]

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