SULIT 3 4551/3

Answer all questions.

1. A group of students carried out an experiment to study the anaerobic respiration in yeast.
Diagram 1.1 shows the set-up of the experiment.

Delivery tube Delivery tube

Thermometers

Stoppers

Test tube Boiling Test tube

A tubes B

Paraffin
Lime oil Lime
water water

Set A Set B

5 cm3 yeast suspension 5 cm3 distilled water

+ 5 cm3 boiled + 5 cm3 boiled
glucose solution glucose solution

Diagram 1.1

The thermometer readings and the observations on the lime water were taken at 0 minute and
30 minutes.

Diagram 1.2 shows the observations made on the lime water.

Lime Lime
water water
(clear) (chalky)

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Diagram 1.2

Table 1.3 shows the result of the experiment at 0 minute and 30 minutes.

Time: 0 minute

Set A Set B

ºC ºC
40 40

30 30

20 20

Thermometer reading Thermometer reading

31 ºC 29 ºC

Time: 30 minutes

Set A Set B

ºC ºC
40 40

30 30

20 20

Thermometer reading Thermometer reading

37 ºC 29 ºC

Table 1.3

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For examiner’s
use

1 (a)
(a) State the thermometer readings in the spaces provided in Table 1.3. [3 marks]
For
examiner’s
use

(b) (i) Based on Table 1.3, state two observations that can be made on Set A in the
experiment. [3 marks]

Observation 1 on Set A:

At 30 minutes, the thermometer reading is 37 ºC, higher than at time 0

minute / has increased.

Observation 2 on Set A:

At 0 minutes, the lime water has not changed into chalky, compared to 30
minutes.

(ii) State the inference for each observation made in (b) (i). [3 marks]
1 (b) (i)
Inference for observation 1 on Set A:
At 30 minutes, the temperature increased because heat was produced from
the respiration of yeast / anaerobic respiration of yeast.

Inference for observation 2 on Set A:

At 0 minutes, the CO2 content in the lime water had not increased from
the respiration of yeast / anaerobic respiration of yeast.

(c) Complete Table 1.4 to show the variables involved in the experiment and how the
variables are operated. [3 marks]

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1 (d)
(c)

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1 (c)
Variables Operating the variables
Manipulated variable:
How to alter the manipulated variable:
The presence of yeast
Set A with yeast but Set B without yeast
…………………………....
………………………………..……………………
……………………………
……………………………………………………

Responding variable: How to determine the responding variable:

Production of heat /
increase in temperature,
and production of CO2 /
change in lime water
Controlled variable:
Volume / concentration of
boiled glucose solution //
Anaerobic condition
Recording the thermometer reading in ºC, and
the change in lime water.
………………………………..……………………
How to maintain the controlled variable:
Using the same volume / concentration of
boiled glucose solution // Anaerobic condition
(for both sets)
………………………………………………

Table 1.4

(d) State the hypothesis for this experiment.

Yeast (V1) carries out anaerobic respiration (R) which produced heat / energy
and carbon dioxide (V2).
………………………………………………………………………………………
[3 marks]

(e) (i) Construct a table and record the results of the experiment.
[3 marks]

1 (e) (i)

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For
examiner’s
(ii) Explain the relationship between the boiled glucose solution and paraffin oil use
used in the experiment of the anaerobic respiration process in yeast.
For
…………………..…………..…………………………………………………... examiner’s
use
…………………………………………………………………………………… 1 (e) (ii)
……………………………………………………………………………………
[3 marks]

(f) Following are some of the apparatus and materials used in the experiment.

Yeast suspension, boiled glucose solution, distilled water,

thermometers, lime water, and paraffin oil

.
Categorise the apparatus and materials according to their roles in operating the
variables in the experiment. [3 marks]

1 (f)

(g) Based on the experiment, define operationally anaerobic respiration.

………………………………………………………………………………………
……………………………………………………………………………………… 1 (g)
………………………………………………………………………………………
[3 marks]

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(h) The experiment is repeated by using Set C containing 10 cm3 of yeast suspension
and 10 cm3 of boiled glucose solution.

(i) Predict the readings on the thermometer used in the experiment.

Explain your answer.

……..………..…………..………………………………………………………..
…………………………………………………………………………………… 1 (h) (i)
…………………..…………..
……………………………………………………………………………………
……………………………………………………
[3 marks]

(ii) Explain the time taken for the lime water to change.

…………………..…………..……………………………………………………
…………………………………………………………………………………… 1 (h) (i)
…………………..…………..……………………………………………………
[3 marks]

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2.
Aquatic plants carry out photosynthesis to obtain food.
In the process, oxygen bubbles are released to the
aquatic environment.
The rate of photosynthesis is affected by several
environmental factors such as temperature, light
intensity and concentration of carbon dioxide.

Design an experiment to determine the effect of carbon dioxide concentration on the

rate of photosynthesis.

(17 marks)

END OF QUESTION PAPER

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SPACE FOR ANSWERS For examiner’s

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SPACE FOR ANSWERS For examiner’s

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SPACE FOR ANSWERS For examiner’s

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SPACE FOR ANSWERS For examiner’s

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