The Kelsey-Sykes test is a triple challenge test used to determine effective disinfectant concentrations under clean and dirty conditions. It involves three successive additions of bacteria to the disinfectant over 30 minutes. The disinfectant concentration is reduced by half with each addition of organic matter. Growth is assessed after incubation to determine if the disinfectant passed or failed. It provides guidelines for disinfectant dilutions but is complicated to perform.
The Kelsey-Sykes test is a triple challenge test used to determine effective disinfectant concentrations under clean and dirty conditions. It involves three successive additions of bacteria to the disinfectant over 30 minutes. The disinfectant concentration is reduced by half with each addition of organic matter. Growth is assessed after incubation to determine if the disinfectant passed or failed. It provides guidelines for disinfectant dilutions but is complicated to perform.
The Kelsey-Sykes test is a triple challenge test used to determine effective disinfectant concentrations under clean and dirty conditions. It involves three successive additions of bacteria to the disinfectant over 30 minutes. The disinfectant concentration is reduced by half with each addition of organic matter. Growth is assessed after incubation to determine if the disinfectant passed or failed. It provides guidelines for disinfectant dilutions but is complicated to perform.
Is a triple challenge test, designed to determine concentrations of disinfectant that will be
effective in clean and dirty conditions. The disinfectant is challenged by three successive additions of a bacterial suspension during the course of the test. The duration of test takes over 30 minutes to perform. The concentration of the disinfectant is reduced by half by the addition of organic matter (autoclaved yeast cells), which builds up to a final concentration of 0.5%. Depending on the type of disinfectant, a single test organism is selected from S. aureus, P. aeruginosa, P. vulgaris and E. coli. The method can be carried out under 'clean' or 'dirty' conditions. The dilutions of the disinfectant are made in hard water for clean conditions and in yeast suspension for dirty conditions. Test organism alone or with yeast is added at 0, 10 and 20 minutes interval. The contact time of disinfectant and test organism is 8 min. The three sets of five replicate cultures corresponding to each challenge are incubated at 32o C for 48 hours and growth is assessed by turbidity. The disinfectant is evaluated on its ability to kill microorganisms or lack of it and the result is reported as a pass or a fail and not as a coefficient. Sets that contain two or more negative cultures are recorded as a negative result. The disinfectant passes at the dilution tested if negative results are obtained after the first and second challenges. The third challenge is not included in the pass/fail criterion but positive cultures serve as inbuilt controls. If there are no positive cultures after the third challenge, a lower concentration of the disinfectant may be tested.
The capacity test of Kelsey and Sykes gives a good guideline for the dilution of the preparation to be used. Disadvantage of this test is the fact that it is rather complicated.
Prueba de Kelsey-Sykes Es una prueba triple desafo, diseado para determinar las concentraciones de desinfectante que ser efectiva en condiciones limpias y sucias. El desinfectante es desafiado por tres adiciones sucesivas de una suspensin bacteriana durante el transcurso de la prueba. La duracin de la prueba toma ms de 30 minutos para realizar. La concentracin del desinfectante se reduce a la mitad por el adems de la materia orgnica (clulas de levadura tratadas en autoclave), que se acumula a una concentracin final de 0,5%. dependiendo del tipo de desinfectante, un solo organismo de prueba se selecciona a partir de S. aureus, P. aeruginosa, P. vulgaris y E. coli. El procedimiento puede llevarse a cabo bajo condiciones "limpia" o "sucio". Las diluciones del desinfectante se hacen en agua dura para condiciones de limpieza y en suspensin de levadura para condiciones de suciedad. Organismo de ensayo solo o con levadura se aade a 0, 10 y 20 Intervalo de minutos. El tiempo de contacto de desinfectante y organismo de prueba es de 8 min. Las tres series de cinco cultivos duplicados correspondientes a cada desafo se incuban a 32 C durante 48 horas y de crecimiento se evala por la turbidez. El desinfectante se evala por su capacidad de matar microorganismos o la falta de ella, y el resultado se registra como un pase o una falta y no como un coeficiente. Establece que contienen dos o ms cultivos negativos se registran como un resultado negativo. El desinfectante pasa a la prueba de dilucin si se obtienen resultados negativos despus de las primera y segunda desafos. El tercer desafo no est incluido en el criterio de pasa / no pasa, pero los cultivos positivos sirven incorporado controles. Si no hay cultivos positivos tras el tercer desafo, una concentracin inferior de la desinfectante puede ser probado. La prueba de la capacidad de Kelsey y Sykes da una buena gua para la preparacin de la dilucin para ser utilizado. La desventaja de esta prueba es el hecho de que es bastante complicado.
In-use test: A simple to use test was described by Maurer in 1985 that can be used in hospitals and laboratories to detect contamination of disinfectants. A 1 ml sample of the disinfectant is added to 9 ml diluent which also contains an inactivator. Ten drops, each of 0.02 ml volume of the diluted sample are placed on each of two nutrient agar plates. One is incubated at 37o C for three days and the other at room temperature for seven days. Five or more colonies on either plate indicate contamination.
Prueba en uso: Una prueba simple de usar fue descrita por Maurer en 1985 que se puede utilizar en hospitales y laboratorios para detectar la contaminacin de los desinfectantes. Una muestra de 1 ml del desinfectante se aade a 9 ml de diluyente que tambin contiene un inactivador. Diez gotas, cada una de 0,02 ml de volumen de la diluida muestra se coloca en cada una de dos placas de agar nutriente. Uno se incub a 37C durante tres das y el otro a temperatura ambiente durante siete das. Cinco o ms colonias en la placa o bien indican contaminacin.