Professional Documents
Culture Documents
Objectives
Lab exercises from the laboratory manual are scheduled by the instructor and cover
selected objectives described below:
Exercise 1: Introduction to the Microbiology Lab -- Safety: Use and Care of the
Microscope
1. List the specific hazards that one may encounter in the Microbiology Lab and
describe how to protect oneself from each of those hazards.
2. Demonstrate the correct use of a compound light microscope.
3. Diagram the path of light through a compound light microscope.
4. Name and locate the major parts of a compound microscope.
5. Using prepared slides, locate and identify on oil immersion the three basic
morphologies of bacteria
1. Identify the incubation conditions for each of the following types of organisms:
aerobes, obligate anaerobes, aerotolerant anaerobes, microaerophiles, and
facultative anaerobes.
2. Describe three methods of culturing anaerobes.
3. Using both reducing media and regular media in an anaerobic pouch and in
aerobic conditions, separate a mixed population of bacteria from soil according to
the organisms'
preferences for atmospheric conditions.
1. Using a mixed broth culture of several bacteria, isolate the different bacterial
species from one another by using both the streak plate and the pour plate
methods.
2. Prepare and maintain a pure culture from a mixed population of bacteria.
1. Compare the bactericidal effectiveness of dry heat and most heat on different
species of bacteria
2. Evaluate the heat tolerance of certain microorganisms.
3. Define and provide a use for each of the following: incineration, hot air oven,
pasteurization, boiling, and autoclaving.
4. Given a number of different microorganisms growing in broth, simulate
pasteurization conditions and attempt to determine the length of time needed to
completely kill each of the different microorganisms.
5. Using your data, evaluate the effectiveness of pasteurization on those organisms.
1. Given a culture of bacteria and a description of a patient situation from which that
organism was taken, identify the organism and discuss whether or not that
organism is likely to be causing that situation in that patient.
2. Determine the sensitivity of that organism to chemotherapeutic agents.