IF PHARMACY

Group no. 5
Members:
Lopez, Princess Fay S.
Lozano,Juvy Anne A.
Maternal, Charles Justin P.
Miranda, Joyce Anne T.
Mondoedo, Samantha Louise S.


Experiment no. 18
Cellular Respiration: Release of Carbon Dioxide in Respiration

Procedure:


Results:




Wrap two dozens of germinating mongo seeds in two
separate gauzes and tie each with a thread.
Suspend the tulle bag containing the seedings inside
a flask with 100 ml water. Suspend the other inside a
flask containing 100 ml Calcium hydroxide solution.
Wrap both flasks with carbon paper.
Observe which flasks formed white precipitate after
two days.

Discussion:

The carbon dioxide produced from the respiration of the mongo seed was used to show chemical reactions
which demonstrated its presence. The reaction of Ca(OH)
2
and CO
2
produced a white precipitate or
chemically known as calcium carbonate which was insoluble in water. The equation for the reaction is:

Ca(OH)
2
+ CO
2
CaCO
3
+ H
2
O
Base on the given chemical reaction, it is evident that there was carbon dioxide (CO
2
) released as calcium
hydroxide (Ca(OH)
2
) reacts with it forming a precipitate of calcium carbonate (CaCO
3
) which looks like milky
in a process called carbonatation. It forms a precipitate due to the cations and anions of aqueous solutions
combine to form an insoluble ionic solid which also known as the precipitation reaction.
Additional info:
The set-up was covered with carbon paper to avoid non-significant reactions of light with the experiment,
which may counteract with the release of carbon dioxide on the body. It was also covered by Erlenmeyer
stopper so that no gas would go in, and no gas inside the set-up would escapefrom the flask.
As far with growth and metabolism of germinated mung bean or the mongo, theres no significant changes
observed. This is because of non-adaptive environment of the seed inside the flask. The mung bean was
actually an example of C3 plant. These are plants that utilizes the C
3
carbon fixation pathway as the sole
mechanism to convert CO
2
into an organic compound (i.e. 3-phosphogylycerate). C3 plants are usually
administered with photorespiration. Photorespiration is the process of respiration that consumes oxygen
and releases CO2 in the presence of light that does not produce ATP, consumes the reducing power for
reducing O2 to CO2, reduces photosynthetic efficiency and occurs in C3 plants.
Conversion of RuBP in Photorespiration
Note: RuBP is ribulosebiphosphate. It's purpose is in the dark reaction. It is formed by taking a phosphate,
coming from the splitting of ATP, and joining it with RuP, changing RuP (ribulosephospate) into RuBP. RuBP
is then able to join with CO
2
and form an unstable 6C molecule which is the basis for the dark reaction.



Base on the given conversion of RuBP under photorespiration, theres a release of carbon dioxide as we add
an atom of oxygen on phosphoglycolic acid (O
2
) through peroxisomes. This phenomena would also explain
why carbon dioxide released.







Reference/s:
J. Jeanty (n.d). Cellular respiration in germinating seeds. Retrieved August 14, 2013
http://www.ehow.com/list_7531578_cellular-respiration-germinating-seeds.html

C. Lee (n.d). Chap.4 Growth and Metabolism. Retrieved August 14, 2013 from
http://www.ndsu.edu/pubweb/chiwonlee/plsc210/topics/chap4
growth/chapt%204%20growth%20and%20metabolism%20slides%20pdf%20file.pdf

C3 Plant (n.d). In Biology online. Retrieved August 14, 2013 from http://www.biology
online.org/dictionary/C3_plant

Calcium Hydroxide (n.d).In Wikipedia.Retrieved August 14, 2013 from
http://en.wikipedia.org/wiki/Calcium_hydroxide

Precipitation Reactions (n.d).In Chemwiki.Retrieved August 14, 2013 from
http://chemwiki.ucdavis.edu/Inorganic_Chemistry/Reactions_in_Aqueous_Solutions/Precipitation_Reactio
ns

RuBP (n.d).In Answers.Retrieved August 14, 2013 from
http://wiki.answers.com/Q/What_is_RuBP_and_what_is_its_purpose_in_photosynthesis

Cellular Respirations (n.d).In Community college of Indiana.Retrieved August 14, 2013 from
http://faculty.ivytech.edu/~twmurphy/txt_202/cell_res.htm

Cobar, M.A.C., Laurente, O., & Vasquez, R. (2013).Laboratory Manual For Pharmaceutical
Botany.Manila:UST.Mauseth, J. (2012). Botany: An Introduction to Plant Biology. Minesotta:Jones
and Bartlett Publisher, LLC.