Rhizobia, though thought to be solely soil saprophytes, can also be found in aquatic systems. Agri-cultural practices, migration of birds and animals, atmospheric deposition of soil particles. Commercial inoculants achieve inoculation rates of 10 3 -10 6 rhizae seed -1.
Rhizobia, though thought to be solely soil saprophytes, can also be found in aquatic systems. Agri-cultural practices, migration of birds and animals, atmospheric deposition of soil particles. Commercial inoculants achieve inoculation rates of 10 3 -10 6 rhizae seed -1.
Rhizobia, though thought to be solely soil saprophytes, can also be found in aquatic systems. Agri-cultural practices, migration of birds and animals, atmospheric deposition of soil particles. Commercial inoculants achieve inoculation rates of 10 3 -10 6 rhizae seed -1.
Habitat Rhizobia through their ability to x N 2 in symbi- osis with legumes play a central role in the N supply of most natural ecosystems. The Ameri- can tall grass prairie is but one ecosystem in which plant diversity and productivity is con- trolled in large measure by N availability (Col- lins et al., 1998). Rhizobia, although thought to be solely soil saprophytes, can also be found in aquatic systems associated with water-growing leguminous plants. Owing to cultural and agri- cultural practices, the migration of birds and animals, and atmospheric deposition of soil par- ticles, there are relatively few soils in the world that do not contain some rhizobia. Rhizobia have been shown to exist in soils for a relatively long time in the absence of a host plant (Bottomley, 1992; Brunel et al., 1988; Kucey and Hynes, 1989; Sanginga et al., 1994; Slattery and Coventry, 1993; Weaver et al., 1972). Rhizobia have been recognized as being important for the functioning of soil ecosystems for centuries (Fred et al., 1932). Shortly, after legume root nodules were shown conclusively to assimilate atmospheric N 2 (Hellriegel and Wilfarth, 1888), Nodbe and Hiltner applied for, and were granted, a patent for the use of these microorganisms as legume inoculants (Elkan and Bunn, 1994). This and subsequent farming and cultural practices have led to the dissemina- tion of rhizobia on a global basis. Rhizobia in soils may be introduced by appli- cation of commercial inoculants or, as in many cases, be the normal ora present as microsym- bionts of an indigenous legume. Inoculants applied to seed, as recommended by their man- ufacturer, achieve inoculation rates of 10 3 10 6 rhizobia seed -1 (Somasegaran and Hoben, 1994). This corresponds to application rates of up to 8 10 10 rhizobia ha -1 (Brockwell and Bottomley, 1995). At these rates, inoculant strains often dominate in nodulation in the rst year of a newly introduced crop (Brockwell et al., 1982; Gibson et al., 1976; Singleton and Tavares, 1986). Moreover, inoculant strains contribute to the rapid buildup of rhizobia in the soil once nod- ulessenesce and release large numbers of viable rhizobia into the soil system (McDermott et al., 1987; Sutton, 1983). Several studies have docu- mented that inoculant strains dominate in nod- ules 515 years after initial inoculation (Brunel et al., 1988; Diatloff, 1977; Lindstrom et al., 1990). It should be noted, however, that not all introduced legumes receive inoculation, and in such situations, seed, soil or aerial contamination will usually lead to some initial nodule forma- tion, and over a period of 45 years, a buildup of soil rhizobial populations (Sadowsky and Graham, 1998a). Moreover, diverse rhizobial populations can develop in association with species that are not initially indigenous to a particular region (Leung et al., 1994). Although it is thought that rhizobia in soil have a clonal Table 2. Differences among genera of root nodule bacteria in the carbon compounds used for growth 1 . Symbols: +, positive reaction; -, negative reaction; +/-, discriminatory within the genus; (+), mainly positive reaction; (-), mainly negative reaction. 1 Includes data from Elkan and Brunn, 1992; de Lajudie et al., 1994, 1998; Rome et al., 1996; Jarvis et al., 1997 and Wang et al., 1999. Genus of nodule bacteria Carbon source Rhizobium Sinorhizobium Mesorhizobium Allorhizobium Bradyrhizobium Azorhizobium Adonitol + + + - - D-Arabinose + + + - + - L-Arabinose (+) + + - D-Cellobiose + + + - - L-Fucose + +/- +/- - - - Inositol + + +/- + - - Gluconate + (+) - + + Lactose + + (+) + - - L-Lysine +/- (-) - - - DL-Malate (+) (+) +/- + (+) + D-Maltose + + + + - - D-Mannose + + (+) + + - Mannitol + (+) + + (+) - D-Mellibiose + + (-) - - D-Rafnose + + +/- - - - Ribose + + + + - L-Rhamnose + + + + (+) - Sucrose + + + (-) + - Trehalose + + + (-) (+) - D-Xylose + + + - + -