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Lesson Plan

To engage the students in learning about Villin we have designed an activity whereby the
students need to compute the molar mass of Villin.
There is a teachers version of the activity and there is a students version.
In summary, the activity requests that the students compute the molar mass of villin.
Students will have to add the molar mass of each amino acid forming the villin polymer
and subtract for the water molecules that leave the polymer as a result of peptide bonds
being formed.
This activity will be introduced after the students have built the snack model, so as to be
more familiar with the various amino acids and the villin protein.
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Molar Mass of Villin: Teachers edition
Subject: High School Chemistry
California State Standard addressed as part of this actiity
Conseration of Matter and Stoichiometry
$. The conservation of atoms in chemical reactions leads to the principle of conservation
of matter and the ability to calculate the mass of products and reactants. %s a basis for
understanding this concept&

d. Students know how to determine the molar mass of a molecule from its chemical
formula and a table of atomic masses and how to convert the mass of a molecular
substance to moles, number of particles, or volume of gas at standard temperature and
pressure.
!rganic Chemistry and "iochemistry
!'. The bonding characteristics of carbon allow the formation of many different organic
molecules of varied si(es, shapes, and chemical properties and provide the biochemical
basis of life. %s a basis for understanding this concept&
a. Students know large molecules )polymers*, such as proteins, nucleic acids, and starch,
are formed by repetitive combinations of simple subunits.
b. Students know the bonding characteristics of carbon that result in the formation of a
large variety of structures ranging from simple hydrocarbons to comple+ polymers and
biological molecules.
c. Students know amino acids are the building blocks of proteins.
f., Students know the -.group structure of amino acids and know how they combine to
form the polypeptide backbone structure of proteins.
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#ntroduction
In this activity students will be asked to compute the molar mass of Villin. To complete
the activity student will have to know how to compute the molar mass of a chemical
compound and to compute percentage by mass and by number of the various amino acids
that make up villin. Students will also have to know how to account for the condensation
bonds that occur between contiguous amino acids and to subtract the molar mass of the
molecules of water that are formed in the peptide bonds.
$e%uired time to complete actiity& / hours
Planning time: ! hour to make copies of students worksheet and a couple of overhead
transparencies
Material needed: paper, pen, calculator, periodic table
!bjectie:
0nowing that villin is made of the following sequence of amino acids
12130%V345T-S%3%677#08867002047357S
!. "ompute the molar mass of villin.
/. "ompute the percentage by number of each atom component.
$. "ompute the percentage by mass of each atom component.
Step &:
Identify the components of each amino.acid and the number of times that each amino
acid appears in the Villin protein
1 , / 9 / , ):,; < :," < =,><6* 9 ?; < ?" < !/> < /6
2 , / 9 /, )@,"<?,><:,;<6* 9 !'" < != > <?; < /6
3 , : 9 : , )A,"<!!,><6</,;* 9 $=" < ::> <:6 < ?;
0 , @9 @ , )=,"<!:,> </,6 </,;* 9 $'" < B'> <!'6 < !';
% , $ 9 $ , )$ ," < B,>< 6< /,;* 9 A" < /!> <$6 < =;
V , ! 9 !, )@," < !!,> < 6< /,;* 9 @" < !!> < 6 </;
4 , / 9 /, )@," < !!,> < 6 < /,;* 9 !'" < //> < /6 < :;
5 , / 9 / , )@,"< !!,>< 6< /,; < S* 9 !'" < //> </6 <:; < /S
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T , ! 9 !, ): ,"< A,> < 6< $,;* 9 :" < A> < 6 < $;
- , ! 9 ! , )=,"< !:, > < :,6 < /,;* 9 =" < !:> < :6 < /;
S , / 9 / , )$," < B,> < 6 < $ ,;* 9 =" < !:> < /6 < =;
6 , / 9 / , ):," < ?,> < /,6 < $, ;* 9?" < !=> < :6 < =;
7 , @ 9 @ , )= , " < $ , > < 6 < / , ;* 9 $'" < !@> < @6 <!';
, ! 9 ! , ) @ ," < A , > < 6 < / , ;* 9 @" < A> < 6 < /;
# , ! 9 ! , ) !! , " < !/ , > < / , 6 < / , ; * 9 !!" < !/> < /6 < /;
8 , / 9 / , ) @ , " < !' , > < / , 6 < $ , ;* 9 !'" < /'> < :6 <= ;
Step ':
%dd all carbon, o+ygen, hydrogen, nitrogen, sulfur atoms.
This will lead to&
BA ;+ygen atoms
!A? "arbon atoms
$/B >ydrogen atoms
:A 6itrogen atoms
/ Sulfur atoms
Step (:
%ccount for peptide bonds that occur between amino.acids. These bonds cause
water molecules to be condensed away from the amino acid chain.
% peptide bond occurs between two adCacent pairs. There are $= amino acids
linked by $@ peptide bonds. 2ach bond releases one molecule of water. % total of
$@ , >/; molecules of water needs to be subtracted from the above numbers
Total number of >ydrogen atoms 9 $/B D $@,/ 9 /@B >ydrogen atoms
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Total number of ;+ygen atoms 9 BA D $@ 9 :: ;+ygen atoms
Step ):
"ompute molar mass of Villin knowing that&
5olar mass of " 9 !/gEmol
5olar mass of ; 9 !=gEmol
5olar mass of > 9 !gEmol
5olar mass of 6itrogen 9 !:gEmol
5olar mass of Sulfur 9 $/ gEmol
2ach molecule of Villin contains&
:: ;+ygen atoms
!A? "arbon atoms
/@B >ydrogen atoms
:A 6itrogen atoms
/ Sulfur atoms
2ach mole of Villin will contain the above number of moles of the respective
atoms. #e can now compute the molar mass of one mole of Villin&
:: mol ;, !=gEmol ; < !A? mol " , !/ gEmol " < /@B mol > , !gEmol > < :A
mol 6 , !: gE mol 6 < / mol S ,$/ gEmol 9 )*+, g-mol
Step .
"ompute the percentage by number of each atom component.
The total number of atoms that make up a Villin molecule can be easily obtained
adding the number of the respective ;+ygen >ydrogen "arbon, 6itrogen and
Sulfur atoms&
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:: < !A? < /@B < :A < / 9 @@' atoms.
ercentage >ydrogen E Total atoms 9 /@BE@@' , !'' 9 :=.B/F
ercentage ;+ygen E Total atoms 9 ::E@@' ,!'' 9 ?F
ercentage "arbon E Total atoms 9 !A?E@@' ,!'' 9 $=F
ercentage 6itrogen E Total atoms 9 :AE@@' , !'' 9 ?.A F
ercentage Sulfur E Total atoms 9 /E@@' ,!'' 9 .$=F
Step /
"ompute the percentage by mass of each atom component.
The molar mass of a Villin mole was computed in Step : as :'?B gEmol
ercentage by mass of >ydrogen E Total mass 9 /@B mol > , ! gEmol> E:'?B
gEmol Villin , !'' 9 =./AF
ercentage by mass of ;+ygen E Total mass 9 :: mol ;, != gEmol; E:'?B gEmol
Villin ,!'' 9 !B.//F
ercentage by mass of "arbon E Total mass 9 !A? mol " , !/ gEmol "E:'?B gEmol
Villin ,!'' 9 @?.!:F
ercentage by mass of 6itrogen E Total mass 9 :A mol 6 , !: gEmol 6E:'?B
gEmol Villin , !'' 9 !=.B? F
ercentage by mass of Sulfur E Total mass 9 / mol S , $/ gEmol SE:'?B gEmol
Villin ,!'' 9 !.!$ F
"heck for correctness by adding up all percentages&
F > 9 =./AF
F ; 9 !B.//F
F " 9 @?.!:F
F 6 9 !=.B?F
F S 9 !.!$F
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=./A < !B.!!< @?.!: < !=.B? < !.!$ 9 AA.:@ F )this is because of rounding, as
we rounded the molar masses of each element*
Molar Mass of Villin: Students actiity
!bjectie:
0nowing that villin is made of the following sequence of amino acids
12130%V345T-S%3%677#08867002047357S
"ompute the molar mass of villin.
"ompute the percentage by number of each atom component.
"ompute the percentage by mass of each atom component.
Step &:
Identify the components of each amino.acid and the number of times that each amino
acid appears in the Villin protein. The ne+t few pages give the structure and composition
of each amino acid.
D Aspartic Acid
4 oxygen
4 carbon
6 hydrogen
1 nitrogen
Aspartic acid,
Letter: D
Abbreviation:
Asp
Looks Info Nae
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! "#$taic Acid
% carbon
& hydrogen
4 oxygen
1 nitrogen
"#$taic acid
Letter: !
Abbreviation:
"#$
Looks Info Nae
' (heny#a#anine
) carbon
11 hydrogen
1 nitrogen
* oxygen
(heny#a#anine
Letter: '
Abbreviation:
(he
Looks Info Nae
+ Lysine
6 carbon
14 hydrogen
* nitrogen
* oxygen
Lysine
Letter: +
Abbreviation:
Lys
Looks Info Nae
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A A#anine
, carbon
- hydrogen
1 nitrogen
* oxygen
A#anine
Letter: A
Abbreviation:
A#a
Looks Info Nae
. .a#ine
% carbon
11 hydrogen
1 nitrogen
* oxygen
.a#ine
Letter: .
Abbreviation:
.a#
Looks Info Nae
" "#ycine
% carbon
11 hydrogen
1 nitrogen
* oxygen
"#ycine
Letter: "
Abbreviation:
"#y
Looks Info Nae
age A "laudia #inkler
/ /ethionine
% carbon
11 hydrogen
1 nitrogen
* oxygen
1 s$ph$r
/ethionine
Letter: /
Abbreviation:
/et
Looks Info Nae
0 0hreonine
4 carbon
) hydrogen
1 nitrogen
, oxygen
0hreonine
Letter: 0
Abbreviation:
0hr
Looks Info Nae
1 1erine
, carbon
- hydrogen
1 nitrogen
, oxygen
1erine
Letter: 1
Abbreviation:
1er
Looks Info Nae
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2 0ryptophan
11 carbon
1* hydrogen
* nitrogen
* oxygen
0ryptophan
Letter: 2
Abbreviation:
0rp
Looks Info Nae
3 "#$taine
% carbon
14 hydrogen
* nitrogen
, oxygen
"#$taine
Letter: 3
Abbreviation:
"#n
Looks Info Nae
"ount all the atoms that make up villin, by element. To make this task easier, count the
individual amino.acids that make up villin and figure out how many times each of them
appears.
0nowing that villinss sequence of amino acids is&
12130%V345T-S%3%677#08867002047357S
Identify all common amino acids and count all atoms. Show your work in a separate sheet
of paper.
>ere is a hint&
1 , / 9 / , ):,; < :," < =,><6* 9 ?; < ?" < !/> < /6
2 , / 9 /, )@,"<?,><:,;<6* 9 !'" < != > <?; < /6.
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Step ':
%dd all carbon, o+ygen, hydrogen, nitrogen, sulfur atoms.
This will lead to&
GGGG ;+ygen atoms
GGGG "arbon atoms
GGGG >ydrogen atoms
GGGG 6itrogen atoms
GGGG Sulfur atoms
Step (:
%ccount for peptide bonds that occur between amino.acids. These bonds cause
water molecules to be condensed away from the amino acid chain.
% peptide bond occurs between two adCacent pairs. There are $= amino acids
linked by $@ peptide bonds. 2ach bond releases one molecule of water. % total of
GGGG , >/; molecules of water needs to be subtracted from the above numbers
Total number of >ydrogen atoms 9 GGGG
Total number of ;+ygen atoms 9 GGGG
Step ):
"ompute molar mass of Villin knowing that&
5olar mass of " 9 GGGG
5olar mass of ; 9 GGGG
5olar mass of > 9 GGGG
5olar mass of 6itrogen 9 GGGG
5olar mass of Sulfur 9 GGGG
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2ach molecule of Villin contains&
GGGG ;+ygen atoms
GGGG "arbon atoms
GGGG >ydrogen atoms
GGGG 6itrogen atoms
GGGG Sulfur atoms
2ach mole of Villin will contain the above number of moles of the respective
atoms. #e can now compute the molar mass of one mole of Villin&
GGGGGGGGGGGGGGGGGGGGGGGGGGGG
Step .
"ompute the percentage by number of each atom component.
The total number of atoms that make up a Villin molecule can be easily obtained
adding the number of the respective ;+ygen >ydrogen "arbon, 6itrogen and
Sulfur atoms&
GGGG
ercentage >ydrogen E Total atoms 9 GGGG
ercentage ;+ygen E Total atoms 9 GGGG
ercentage "arbon E Total atoms 9 GGGG
ercentage 6itrogen E Total atoms 9 GGGG
ercentage Sulfur E Total atoms 9 GGGG
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Step /
"ompute the percentage by mass of each atom component.
The molar mass of a Villin mole was computed in Step : GGGG
ercentage by mass of >ydrogen E Total mass 9 GGGG
ercentage by mass of ;+ygen E Total mass 9 GGGG
ercentage by mass of "arbon E Total mass 9 GGGG
ercentage by mass of 6itrogen E Total mass 9 GGGGGGGG
ercentage by mass of Sulfur E Total mass 9 GGGG
"heck for correctness by adding up all percentages&
F > 9 GGGG
F ; 9 GGGG
F " 9 GGGG
F 6 9 GGGG
F S 9 GGGG
Is your total equal !''FH 1iscuss reasons possible sources of error if your total
sum differs from !'' by more than /F.
#hat did you learn in this labH

age !: "laudia #inkler