Effect of pH on urease

By: Nique Bonus Guitar Tem Rerk 10-5

Introduction
the experiment is trying to examine how pH effect
on urease enzyme activity and to test the function of
urease enzyme in difference condition (pH, temperature
or ionic condition) and observe how the function of the
enzyme change in the certain condition.
We predict that the pH of the solution will affect the
rate of activity. Only the proper rate of pH will give the
best result. Other than that, the enzyme will be
denatured.
Result
before after
next 5
minus
tube 1 2 2.8 2.2
tube 2 3 4.8 5.4
tube 3 4 9.5 8.3
tube 4 7 10.14 8.9
tube 5 8 9.93 11.6
procedure 1
1.Why is it important to have the three different tube?
Answer: To make sure that the indicator can work in all
condition (acid, base, and neutral).
2.Can you predict what colour of the solution will be if the
urease enzyme convert urea to ammonia?
Answer: The solution which is acid will change to red, and
base will change to blue but the neutral solution will
remain the same colour.
procedure 2
1. what are the purpose of procedure2?
Answer: To check if the enzyme still working when we add the
soya suspension.
2. why did we need to add an enzyme in to a test tube without
urea even though we can predict that no enzyme activity will
occur?
Answer: we need to add an enzyme into a test tube with out urea
in order to see the different when the enzyme activity occur and
proof that the enzyme is really work.
Effect of pH on urease
1.How does pH effect on urease activity? How do you do?
Answer: The urease enzyme function will work more efciently
in certain pH that we can know by measure the pH and the
change of Colour.
2.From your result, what pH would you use and avoid if you
have to conduct an experiment using urease enzyme and why?
Answer: According to the result, we would use the substance
which is have the pH around 4 and avoid using substance which
have the pH more than 7 because it will denature.
Effect of pH on urease
3. Because basic pH will also change the colour of the
indicator to blue/green which is very similar when
ammonia has been produced by the urease enzyme,
how can we make sure that the colour change is
actually due to the urease activity? Is it possible
we can make sure that the colour change is actually due
to the urease activity by measure the pH twice (before
and after add urea solution) in order to make the result
of the experiment more accurate.
Conclusion
After the experiment, we have learnt that enzyme will work most efcient at the
certain level of pH. If the pH level is too high or too low, enzyme can denature and
make it unable to bind substrate into another shape. Based on our hypothesis which said
that pH is the one factor that affect rate the activity that will occur. From the result,
the enzyme was more likely able to work in more base condition. On the other hand, in
acidic solution, the enzyme was denatured. The effect can be observed through each test
tube. In tube that was in acidic conditions, there was a white solid curd under the tube.
For the base solution, the colour that was changed too was same as the subjected
solution from procedure 2. After we compare, it obviously shows that pH affect rate of
activity. There is some error in the experiment which is we havent count the number of
citric acid solution and sodium bicarbonate correctly. We just observe base on the colour
of the solution. Therefore, we should count the amount of substance that will be used in
the experiment certainly
THANK YOU