ABSTRAK

Latar belakang : Plumbum (Pb) dalam darah pada kadar tertentu dapat
menimbulkan gangguan kesehatan di berbagai organ tubuh manusia, salah satunya
sistem hematopeotik. Pb dalam kadar toksik tertentu menyebabkan defisiensi
enzim G-6PD dan penghambatan enzim pirimidin-5-nukleotidase sehingga
terjadi akumulasi RNA serta ribosom yang ditandai dengan adanya eritrosit
basophilic stippling. Keracunan Pb dapat diatasi dengan pemberian madu yang
terbukti memiliki berbagai komposisi antioksidan yang kuat.
Tujuan : Untuk mengetahui pengaruh pemberian madu terhadap gambaran
basophilic stippling eritrosit mencit setelah diberi Pb asetat.
Metode : Desain yang digunakan dalam penelitian ini adalah penelitian
eksperimental dengan tiga kelompok hewan percobaan mencit jantan (Mus
musculus). Populasi dari penelitian ini adalah mencit jantan umur 6-8 minggu
dengan berat 30-50gr dan sehat yang ditandai dengan gerakan aktif. Kemudian
dibagi menjadi tiga kelompok. Kelompok I (kontrol) diberikan aquadest.
Kelompok II diberikan Pb asetat 100mg/kgBB/hari dengan cara dicekok
menggunakan jarum gavage. Kelompok III diberikan Pb asetat 100mg/kgBB/hari
dan madu 0,04 mL madu/20 gram mencit/hari yang diencerkan hingga 0,2 cc
secara bersamaan Setelah 8 minggu, hewan percobaan diaspirasi sedikit darah dari
vena ekor mencit dan dijadikan sampel darah. Kemudian, sampel darah tersebut
langsung dibuat menjadi sediaan hapusan. Jumlah eritrosit basophilic stippling
dihitung dibawah mikrokop. Data yang diperoleh di analisa dengan uji Mean-
Whitney Test.
Hasil : Hasil penelitian menunjukkan adanya erythrocyte basophilic stippling
pada kelompok K2 dan K3. Hasil statistik dengan Uji Kruskal- Wallis yang
dilanjutkan dengan uji Mean-Whitney menunjukkan adanya perbedaan secara
signifikan antara kelompok K2 dan K3 (p<0.001).
Kesimpulan : Dari hasil penelitian ini disimpulkan madu dapat menurunkan
toksik Pb terhadap eritrosit yang berfungsi sebagai alat transportasi oksigen.
Kata kunci : Pb, madu, mikroskopik, eritrosit basophilic stippling.



Universitas Sumatera Utara

ABSTRACT
Background : Lead (Pb) in the blood at a certain level can cause health problems
in various human organs, including hematopoetic system. Pb at the toxic level
causes the G-6PD deficiency and the inhibition of pirimidine-5-nucleotidase so
the RNA and ribosome become acumulated marked with the finding of the
basophilic stippling erythrocytes. The toxicity of Pb can be overcome by the
administeration of honey which has been proved to contain various compositions
of strong antioxidant.
Objective : to find out the affect of the honey to the presentation of the mices
basophilic-stippling erythrocyte after exposed by lead.
Methods : The design used in this study was experimental with three groups of
male mice (Mus musculus). The population of this study was 6-8 week old male
mice weighing 30-50gr and healthy that was marked with an active movement and
then devided into 3 groups. Groups I (control) was given aquadest. Group II was
given lead acetate 100mg/kgBW/day orally using a gavage needle. Group III was
given lead acetate 100mg/kgBW/day and honey 0,04 mL of honey/20 gram
mice/day diluted to 0,2 cc at the same time. After 8 weeks, samples were aspirated
some of their blood from their tails vein and made into blood sample. Then, this
blood sample were directly made into the blood smear. The sum of the basophilic
stippling erythrocytes were counted under the microscope. Data were collected
and analyzed with Mean-Whitney test.
Results: The result of this research showed that there were basophilic stippling
erythrocytes in the K2 and K3 groups. The stastical analysis utilizing the Kruskal-
Wallis Test followed with Mean-Whitney Test showed that there was a significant
difference between the K2 and K3 groups (p<0.001).
Conclusion: From the result of this experimental research, can be concluded that
Honey can reduce the adverse effect of the lead toxicity.
Keywords : lead, honey, microscopic, basophilic stippling erythrocytes





Universitas Sumatera Utara