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= = (2)
Where J is the permeate flux in units of volume per membrane area A, at time t, K
m
is the
hydraulic permeability, the viscosity, and P the membrane pressure drop between the
retentate and permeate.
For cross flow ultrafiltration the average trans-membrane pressure drop is:
1
2 M i
P P P = (3)
And the flux through the membrane is
R
M
m gel
P
J
R
= =
+
(4)
Osmotic pressure is zero for isotonic solutions and applies only for membranes with chemical
potential gradients. Equation (4) becomes
1
R
M
m gel BSA
P
J
R R
=
+ +
(5)
Often the viscosity shown above is normalized and does not show in lumped into the resistance
values. The highest resistance is that for our protein called Bovine Serum Albumin and is very
large compared to the pore sizes and the buffer and water molecules.
9
Bovine Serum Albumin (BSA) is often used as a standard protein marker in the Bradford Protein
Assay test. It in conjunction with a dye of Coomassie Brilliant Blue G-250 allows for
colorimetric analysis of a protein assay based upon the absorbance shift of the dye. BSA is
shown in the Figure below.
Figure 4 - Bovine Serum Albumin (BSA) copied w/o permission Wikipedia.
BSA has a molecular weight of about 66,463 Daltons; Isoelectric Point of 4.7 and a dimension of
about 140x40x40
3
(2.24x10
-25
m
3
). With these physical properties BSA is an acceptable protein
to model other similar proteins with molecular mass of approximately 66,000 Daltons from
(Perry 1999).
The BSA molecular conformation and a near zero a-centric factor helps in the steady state
diffusion to occur rapidly and to have few molecules pass through the DairyUF 10K.
10
III. APPARATUS AND PROCEDURE
Figure 5 - Process & instrumentation diagram for GEA unit.
11
IV. RESULTS AND DISCUSSION OF RESULTS
Since the mass flux equations are correlated for turbulent flow conditions a simple hydraulic
analysis determined the Reynolds number for the geometry of the five plates in parallel was
done. The average retentate flow was used for the volumetric flow for the Reynolds calculation.
Since the flow was through a square duct and the geometry of the area is complex, the hydraulic
radius was calculated. The Reynolds number for flow through a square duct is:
H
Q D
A
(6)
For the determination of the hydraulic radius D
H
, the wetted perimeter and the cross sectional
area are needed. The plate and frame of the membrane is considered to be a section of a
cylinder. The circumference of the cylinder is the location of the perpendicular flow area, and
where the flow would leave the disk. Therefore the area of flow was the thickness of the disk
times the circumference of the cylinder, or
( ) ( )
2
3
16
" 2 " 1.1781in A T C T D = = = = i (7)
The wetted perimeter is
( ) ( )
2
3
16
2 2 2 2 2 12.94in
wetted
P T C = + = + = (8)
and
2
4 4.7123in
0.364in
12.941in
H
A
D
P
= = = (9)
The kinematic viscosity for water was found from Perrys (1999) to be 0.97 cP at 70deg F. This
amounted to an intrinsic viscosity of 0.001508 in
2
/s. Substituting these values into equation (3)
gave a value for the Reynolds number of 2002.59. This value is in the transition region and
additional turbulence is likely added by further geometrical consideration or by the use of
distributors in the plate and frame. However, it should be observed that this should be further
investigated and determined to be in the turbulent region.
The next point of concern was the osmotic pressure. It was assumed to be nearly zero for our
solution. This would be the case for an isotonic solution. For our buffer solution this would be
the case since the molecules could easily diffuse from retentate to permeate side. The buffer was
to help stabilize the BSA solution, but also to help treat and pacify the new membranes. BSA is
mildly negatively charged and the acetic acid and disodium phosphate can act (to some degree)
as a physical absorbed solute on any charged surfaces on the sulonyl membrane pores. This
effect would also be a point of further research.
12
The osmotic pressure for our buffer solution would be expected to be zero since the solution
would be isotonic. The calculation for this pressure is
i
CRT = =
(10)
C is the average concentration of each solute. For our buffer this is
3 1 2
1 2
0.9595+1.866
=0.00441mol/cm
110.72 529.794
n n
C
V V
+
= =
+ +
Substituting the average concentration for the buffer into equation (7)
3 3
(0.00441mol/cm )(82.057atm cm / mol K)(295.65K)(14.6959psi/atm)
1, 573psi
CRT =
=
=
The calculated osmotic pressure is about 1500 psi for a 0.1 M acetic acid and 0.2 M Na
2
HPO
4
buffer solution. This value seems high and for these experiments. Since the change in osmotic
pressure between the retentate side and permeate side was assumed about zero. This value can
be accounted for if there is an equal pressure on the reverse side due to the membrane being
porous. Small molecules such as citric acid and Na
2
HPO
4
.exhibit no net pressure difference due
to them being easily transported between the membrane.
With the addition of BSA to our solution there is a hypertonic case and therefore a pressure
gradient associated with the difference in chemical potentials. The osmotic pressure for the BSA
solution is
7 3 3
3
.002068mol
=1.505x10 mol/cm
13750mL
n
C
V
= =
7 3 3
(1.505x10 mol/cm )(82.057atm cm / mol K)(295.65K)(14.6959psi/atm)
0.0536581161psi
CRT
=
=
=
The value is small when compared to the transmembrane pressure gradient.
The permeate and retentate are isotonic in regards to the buffer solutes. However, in regards to
the BSA concentrations the UF membrane is selective to these large molecules and there should
be a measureable osmotic pressure.
13
(11)
The transmembrane pressure difference was calculated from the following equation. This
equation takes into account pressure drop of solidly in the laminar range. Therefore it can be
concluded that there is a kinethrough thedetermining the Reynolds number for a disk was
equation The osmotic pressure for the buffer solution was determined using the following
equation. It was not clear initially that the osmotic pressures would be negligible.
i
C RT = (12)
In the above equation
i
C is the average concentration for the solution and T is the temperature.
The permeate and retentate are isotonic in regards to the buffer solutes. However, in regards to
the BSA concentrations the UF membrane is selective to these large molecules and there should
be a measureable osmotic pressure.
Figure 6 Pure water permeate flux vs. membrane pressure difference.
y = -0.0339x
2
+ 3.082x - 34.859
R = 0.9968
y = 0.8527x + 21.313
R
2
=1
15.00
17.00
19.00
21.00
23.00
25.00
27.00
29.00
31.00
33.00
35.00
20 22 24 26 28 30 32 34 36 38 40
G
a
l
/
F
t
2
s
P
M
(psi)
Series1
PolyFit
Linear Relaxation
14
Figure 7 shows the pressure drop in psi versus the volumetric flux. The data suggests a non-
linear relationship. However, the membrane is elastic and is not ideal. There is elastic
deformation as the pressure is increase. When the pressure is decrease to the original pressure
value (low pressure) the pressure vs. flux curve will be retained. For this case there was no
buffer or BSA in this run. The data was regressed using least square method. Both a linear and
quadratic fit are shown. The R
2
values are poor, but for our data this case has the best value.
The slope of the linear interpolation is used to determine the bulk permittivity coefficient. The
reciprocal of this slope is the resistivity of the water solution due to the membrane character.
This value was calculated to be 0.8547 lbft
2
s/in
2
gal. This value is normalized with the viscosity
so other comparisons can be made with solutions of difference viscosities
Figure 7 - Buffer permeate flux vs. membrane pressure difference 0.1Mcitric acid 0.2MNa2HPO4
Figure 7 shows the addition of a buffer which was comprised of 0.1M citric acid and 0.2M
disodium phosphate. The addition of the buffer helps maintain pH for the BSA solution, but also
helps remove polarization.
y = 0.752x + 2.0969
R = 0.9672
y = 0.0104x
2
+ 0.209x + 8.1116
R = 0.9927
0.00
5.00
10.00
15.00
20.00
25.00
30.00
35.00
40.00
10 15 20 25 30 35 40 45 50
Flux (gal/ft2*s)
Linear (Flux (gal/ft2*s))
Poly. (Flux (gal/ft2*s))
15
The permittivity of the membrane and the buffer was found to be 0.752 which gives a resistance
of 6.366 lbft
2
s/in
2
gal for the buffer concentration layer. When this normalized with the solution
viscosity the difference is within 20% of the given value.
The addition of low ionic strength buffers helps maximize the electrostatic repulsion between the
negatively charged BSA and the negatively charged membrane. This electrostatic exclusion
causes a dramatic increase in the separation, with relatively little change in the membranes
physical permittivity (permeability).
Figure 8 - BSA permeate flux vs. membrane pressure difference (outliers removed)
Figure 8 shows the BSA solution. The value is 1.4747 lbft
2
s/in
2
gal for the R
bulk
value. The
resistance due to BSA is And when calculated is 6.863 lbft
2
s/in
2
gal and the bulk resistance is
1.47470. lbft
2
s/in
2
gal.
16
Figure 9 Filtration/concentration cycle for BSA
y = 0.0004x
2
+ 0.0038x + 1.3887
R = 0.9881
0.00
0.50
1.00
1.50
2.00
2.50
3.00
3.50
0 10 20 30 40 50 60
w
t
.
%
B
S
A
Time, min
wt. % BSA
Poly. (wt. % BSA)
17
NOMENCLATURE
Nomenclature
Symbol Description Units
A membrane area
m
2
C concentration
kg/m
3
Cb concentration of all retaned species
kg/m
3
C
Ge
concentration of gel
kg/m
3
C
B
concentration of bulk solution
kg/m
3
average transmembrane pressure drop psi
filtrate pressure psi
P pressure psi
k
L
mass transfer coefficient -
J permeate flux on membrane filtration rate -
K
m
membrane hydraulic permeability coefficient -
fluid viscosity cP
membrane pressure drop -
inlet pressure psi
outlet pressure psi
membrane resistance cm
2
/s
gel resistance -
effective diffusivity in liquid film cm2/s
osmotic pressure psi
M
P
M
P
i
P
o
P
M
R
G
R
e
D
f
P
18
REFERENCES
1. Warren L. McCabe, Julian C. Smith and Peter Harriot. Unit Operations of Chemical
Engineering. McGraw-Hill. New York (2005)
2. Kirk and Othmer. Encyclopedia of Chemical Technology. John Wiley & Sons, Inc. New
York (2011)
3. Perry, Robert H., Editor. Perrys Chemical Engineering Handbook. 7
th
Edition.
McGraw-Hill, New York. (1999)
4. Wright AK, Thompson MR (February 1975). Hydrodynamic structure of bovine serum
albumin determined by transient electric birefringence. Biophys. J. 15 (2 Pt 1): 13741.
doi:10.1016/S0006-3495(75)85797-3. PMC 1334600. PMID 1167468.
5. Michael L. Shuler, Fikret Kargi. Bioprocess Engineering Basic Concepts. 2
nd
Edition.
Prentice Hall PTR, New Jersey (2002).
19
APPENDICES
A. LABORATORY DATA
Table 1 - Raw data from experiment on Jan 19, 2012 Patm=12.24psia.
retentate permeate
(gpm) (psia) (psia) (gpm)
1.025 0.051 20.5 1.0 14.3 0.7 0.00745 0.000
- - 29.6 1.5 19.5 1.0 0.02403 -
- - 20.9 1.0 18.0 0.9 0.01618 -
1.574 0.079 18.0 0.9 18.0 0.9 0.01894 0.001
2.097 0.105 31.8 1.6 20.9 1.0 0.02782 0.001
2.348 0.117 34.0 1.7 21.7 1.1 0.03253 0.002
2.878 0.144 41.0 2.0 22.4 1.1 0.05248 0.003
3.396 0.170 43.9 2.2 23.8 1.2 0.04881 0.002
i
P
o
P
20
B. SAMPLE CALCULATIONS
For a plate and frame UF unit the Reynolds number must be in the turbulent range on the
retentate side.
Re =Calculation of the membrane pressure drop is as follows
Calculation of permittivity and resistive coefficient using slope of Table 3 Kw = 0.8527gal/ft2.
m
2 2
lb g
1 2.048
m s ft s
=
1MPa 145.0377psi =
The permittivity of the membrane is the calculated as follows
w w
K K =
7 2
7 2
(1.40686x10 psi s)(0.8527gal/ft )
1.19963 10 gal/ft
W
K
x
=
=
w
K
J P
=
The preceding equation is a form of Darcies equation and is an ideal case. The actual physical
behavior of the membrane pressure changes non-linearly due to compaction and yielding of the
plastic like membrane (Polypropylene-backed Polyethersulfone).
21
C. SPECTROPHOTOMETER CALIBRATION
Figure 10 - Calibration wt% BSA vs. Absorbance
22
D. PUMP CURVE CALIBRATION
Figure 11 - pump curve.
y = 1.2492x
2
- 9.0519x + 21.906
R = 0.9794
0
5
10
15
20
25
0 1 2 3 4
A
x
i
s
T
i
t
l
e
Axis Title
Series1
Poly. (Series1)
23
E. MAJOR ITEMS OF EQUIPMENT
1. GEA Model M membrane Filtration Pilot Plant.
Standard Features
15 liter balance tank with electronic level transmitter
One lab-size spiral wound membrane housing, 1.8" x 12"
One lab-size ceramic membrane housing, 0.75" x 10"
Feed pump with 1 HP variable speed drive
Tubular heat exchanger
Inlet and outlet pressure gauges
Temperature indicator
Manual flow control valves
Flow indicators: one permeate, one concentrate
Assembled on a tubular stainless steel base with casters
Operating Conditions
Pressure up to 6 bar (87 psig)
Temperature up to 93 C (200 F)
Flow up to 1,000 l/h (4.4 gpm)
Utility Requirements
Floor Space 30" x 48" x 65"
Shipping Wt. 350 lbs.
Voltage/ph 220V, 1 ph
Total HP 1
Feed Tank Cap. 19.4 L
Min. Vol. w/Spiral Module Approx. 1.25 L
Min. Vol. w/Other Modules Approx. 0.75 L
Permeate Outlet 3/8" ID FLEX (3/4" TC)
Concentrate Outlet 3/4" ID FLEX (3/4" TC)
Max. Flow 1000 L/H
Max. Pressure 6 bar
Tank Drain 3/4" TC
Tank Bypass 3/4" TC
Heating/Cooling Inlet 1/8" FNPT
Heating/Cooling Outlet 1/8" FNPT
Caster Mounted Yes
2. Plate and Frame stack assembly.
24
Plate and Frame stack assembly (Continued from previous).
E
25
F. ERROR ANALYSIS
For brevities the error analysis was shown to be at max 20%. The errors shown in the graphs are
from laboratories measurement. Error was propagated until the final maximal error of 20% was
calculated.
26
27