UCSF Lab Standard Operating

Procedure
Chemical Process, Name or Hazard Class:
4,4'-Thiodianiline
Please fll out the orm completel!"
#nsure all users ha$e access to this SOP"
%eer to instructions or assistance"
Department: &nter te't"
Date SOP was written: &nter date"
This lab specifc SOP has been reviewed and approved by:
Principal Investiator !ame: &nter te't"
Principal Investiator
Sinat"re
#ab $anaer%S"pervisor: &nter te't"
Type o& SOP: ( Process )Hazardous Chemical ( Hazardous Class
P"rpose
*,*+,-hiodianiline is a potential carcinoen and irritant'
.aterial ma! be irritating to mucous membranes and upper respirator! tract" .a! be atal i
inhaled, s/allo/ed, or absorbed through s0in"
1lso 0no/n as *,*2,.eth!lene,di3o,toluidine4 or *,*2,5iamino,6,62,dimeth!ldiphen!lmethane
7as pre$iousl! used in the production o s!nthetic d!es"
Physical ( )hemical Properties%Defnition o& )hemical *ro"p
C1S8: 96:,;<,9
Class: )arcinoen, Irritant
.olecular Formula: C9=H9=N=S=
Form 3ph!sical state4: solid
Color: bro/n,$iolet po/der
*,*+,-hiodianiline 1 Date: Click here to enter a date.
.odifed rom SOP templates de$eloped b! UCL1 &H>S 3http:??///"sop"ehs"ucla"edu?4
@oiling point: N?1
Potential +a,ards%To-icity
*,*+,-hiodianiline is a potential carcinoen and irritant'
#s harmul b! inhalation, ingestion, or s0in absorption" Can cause e!e and s0in irritation"
.aterial ma! be irritating to mucous membranes and upper respirator! tract" .a! cause
sensitization b! s0in contact"
.a! cause central ner$ous s!stem depression or cardiac disturbances"
.a! cause methemoglobinemia characterized b! chocolate,bro/n colored blood, headache,
/ea0ness, dizziness, breath shortness and c!anosis"
No permissible e'posure limit data is a$ailable"
-he ollo/ing acute to'icit! data is a$ailable:
Oral L5<A ;=A mg?0g BmouseC
Oral L5<A :AA mg?0g BratC
.nineerin )ontrols
Handle using a chemical ume hood /ith good $entilation and electricall! grounded lines and
eDuipment"
Personal Protective ./"ipment 0PP.1
2espirator Protection
# lab personnel /ould li0e to use respirator on a $oluntar! basis, the! must be trained and
ft,tested b! &H>S" -his is a regulator! reDuirement" 3http:??or"ucs"edu?ehs?E9:6,
5SF?$ersion?deault?part?*?data?4
+and Protection
Glo$es must be /orn" Use proper glo$e remo$al techniDue to a$oid an! s0in contact" Nitrile
glo$es are recommended"
NO-&: Consult /ith !our preerred glo$e manuacturer to ensure that the glo$es !ou plan on
using are compatible /ith *,*+,-hiodianiline
%eer to glo$e selection chart rom the lin0s belo/:
http:??///"ansellpro"com?do/nload?1nsellHEth&ditionChemical%esistanceGuide"pd
O%
http:??///"allsaet!products"biz?page?I*9I=
O%
http:??///"sho/abestglo$e"com?site?deault"asp'
O%
http:??///"mapaglo$e"com?
.ye Protection
1NS# appro$ed properl! ftting saet! glasses or chemical splash goggles" Face shield is also
recommended"
*,*+,-hiodianiline 2 Date: Click here to enter a date.
.odifed rom SOP templates de$eloped b! UCL1 &H>S 3http:??///"sop"ehs"ucla"edu?4
S3in and 4ody Protection
Flame resistant lab coats must be /orn and be appropriatel! sized or the indi$idual and
buttoned to their ull length" Laborator! coat slee$es must be o suJcient length to pre$ent
s0in e'posure /hile /earing glo$es" Full length pants and close,toed shoes must be /orn at
all times b! all indi$iduals that are occup!ing the laborator! area" -he area o s0in bet/een
the shoe and an0le should not be e'posed"
+yiene $eas"res
7ash thoroughl! and immediatel! ater handling" %emo$e an! contaminated clothing and
/ash beore reuse"
5irst 6id Proced"res
I& inhaled
.o$e into the resh air immediatel! and gi$e o'!gen" # not breathing gi$e artifcial
respiration" See0 medical attention immediatel!"
In case o& s3in contact
#mmediatel! Kush s0in /ith plent! o /ater or at least 9< minutes /hile remo$ing
contaminated clothing and shoes" 7ash an! contaminated clothing beore reuse" -horoughl!
clean shoes beore reuse" See0 medical attention immediatel!"
In case o& eye contact
Chec0 or and remo$e an! contact lenses" %inse thoroughl! /ith plent! o /ater or at least
9< minutes and consult a ph!sician" See0 immediate medical attention and continue e!e
rinse during transport to hospital"
I& swallowed
5o NO- induce $omiting unless directed b! medical personnel" Ne$er gi$e an!thing b!
mouth to an unconscious person" See0 medical attention immediatel!"
Special +andlin and Storae 2e/"irements
Precautions or sae handling: 1$oid contact /ith s0in and e!es and inhalation" 1$oid
inhalation o $apor or mist" 1$oid ormation o dust"
Conditions or sae storage: Leep in a dr! place" Leep container tightl! closed in a cool, dr!,
and /ell $entilated" Leep a/a! rom incompatible materials and conditions" Leep cool and
protect rom sunlight"
Spill and 6ccident Proced"re
)hemical Spill Dial 7-788 &rom camp"s phone or 489-4:;-8484 &rom cell
phone or 489-<=;->9<< 0S5*+ only1
Spill M 1ssess the e'tent o danger" 1ssist contaminated or inNured persons"
&$acuate the spill area" 1$oid breathing $apors" # possible, confne the spill to a
small area using a spill 0it or absorbent material" Leep others rom entering
contaminated area 3e"g", use caution tape, barriers, etc"4"
*,*+,-hiodianiline 3 Date: Click here to enter a date.
.odifed rom SOP templates de$eloped b! UCL1 &H>S 3http:??///"sop"ehs"ucla"edu?4
Small 0?8 #1 M # !ou ha$e training, !ou ma! assist in the clean,up eOort" Use
appropriate personal protecti$e eDuipment and clean,up material or chemical
spilled" 5ouble bag spill /aste in clear plastic bags, label and ta0e to the ne't
chemical /aste pic0,up"
#are 0@8 #1 M Dial 7-788 &rom camp"s phone or 489-4:;-8484 &rom cell
phone or 489-<=;->9<< 0S5*+ only1 or assistance"
)hemical Spill on 4ody or )lothes M %emo$e clothing and rinse bod! thoroughl!
in emergenc! sho/er or at least 9< minutes" # discomort persists, proceed to the
&mergenc! 5epartment" # no urther discomort is e'perienced, ha$e the S5S
read! and contact Poison Control Hotline at 9,EAA,===,9=== or urther e'posure
inormation" !oti&y yo"r direct s"pervisor and .+(S at 489-4:;-8A== d"rin
wor3 ho"rs, or 7-788 d"rin non-wor3in ho"rs and wee3ends'
)hemical Splash Into .yes M #mmediatel! rinse e!eball and inner surace o
e!elid /ith /ater or 9< minutes b! orcibl! holding the e!e open" # discomort
persists, proceed to the &mergenc! 5epartment" # no urther discomort is
e'perienced, ha$e the S5S read! and contact Poison Control Hotline at 9,EAA,===,
9=== or urther e'posure inormation" !oti&y yo"r direct s"pervisor and .+(S
at 489-4:;-8A== d"rin wor3 ho"rs, or 7-788 d"rin non-wor3in ho"rs
and wee3ends'
$edical .merency Dial 7-788 0camp"s phone1 or 4:;-;788 0cell phone1
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8
hours.
!on-#i&e Threatenin .merencyM Go to Occupational Health Programs 3OHP4
Clinic, *9<,EE<,I<EA, <AA= Post Street, S"ite 4;= +o"rs o& Operation &or
6ppointments: $onday - 5riday ::A= a'm' - 4:== p'm' 0e-cept +olidays1'
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8
hours.
!eedle stic3%p"nct"re e-pos"re 3as applicable to chemical handling procedure4
M 7ash the aOected area /ith antiseptic soap and /arm /ater or 9< minutes" For
mucous membrane e'posure, Kush the aOected area or 9< minutes using an
e!e/ash station" Page the needle stic0 nurse b! dialing 489-A9A-:>4< 0STI)1'
Decontamination%Baste Disposal Proced"re
Clean contaminated suraces /ith soap and /ater and paper to/els" 5ispose o the
paper to/els as hazardous /aste"
*,*+,-hiodianiline 4 Date: Click here to enter a date.
.odifed rom SOP templates de$eloped b! UCL1 &H>S 3http:??///"sop"ehs"ucla"edu?4
Sa&ety Data Sheet 0SDS1 #ocation
Online S5S can be accessed at http:??or"ucs"edu?ehs?I=*9,5SF?msds"html
Protocol%Proced"re
C"antities covered by this SOP:
HHHHHH 3g , ml4 to HHHHHHH 3g, ml4
Temperat"re rane covered by this SOP:
HH PC M HH PC
*eneral Overview and P"rpose:
&nter the e'perimental purpose
Proced"re:
&nter e'perimental procedure" Fou can cop! procedure rom !our lab noteboo0 or rom
literature"
!OT.
1n! de$iation rom this SOP reDuires appro$al rom the Principal #n$estigator"
*,*+,-hiodianiline 5 Date: Click here to enter a date.
.odifed rom SOP templates de$eloped b! UCL1 &H>S 3http:??///"sop"ehs"ucla"edu?4