I N C O R P O R AT I N G

F I S H FA R M I N G T E C H N O L O G Y

July | August 2014

New trials confirm strong results with
probiotics

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FEATURE

NEW TRIALS CONFIRM STRONG

RESULTS WITH

PROBIOTICS
by BioMar

ioMar is at the moment introducing probiotics in still more diets

around the world. The background
is new strong research results
from both sides of the Atlantic Ocean.

In new trial results from Chile the

company can achieve a 40 percent reduction of the occurrence of deformities in
fry as well as improved growth and gut
health for trout fed with its probiotic strain
Pediococcus acidilactici CNCM MA 18/5M
(Bactocell).
Simultaneously, results from trials done in
the United Kingdom indicate a positive effect
of probiotics on the immune response in midsized salmon. And in France trials with a new
Larviva Pro diet suggest a new era in larval
rearing methods can be close.

Improved growth and less

deformities in Chile

Our European colleagues in BioMar and

partners in Lallemand SAS have presented a
very strong documentation for the benefits
of the addition of the unique probiotic strain
Pediococcus acidilactici CNCM MA 18/5M
(Bactocell) to feeds for many different fish
species. Therefore, here in Chile we are
proceeding with the use of Bactocell in our
commercial diets, says Andrs Millan, sales
director of BioMar Chile, is pleased with the
first trial results obtained in Chile, where

Above: The square cages of the BioMars marine trial

facility where the large probiotics trials on salmonids are
carried out are neighbouring the circular net pens of the
commercial Musholm fish farm.

probiotics were introduced in BioMar diets

recently.
The field trials done in Chile with rainbow
trout fry both support the existing theory
and bring new knowledge. The trials were
conducted in collaboration between BioMar
and Aquachile, a major producer of salmon,
coho and trout at the facilities of Aquachile in
Quetroleufu, Chile.
Andrs Millan explains that the results first
of all showed a reduction in the occurrence of
deformities by 40 percent
Deformities are both associated with
mortalities among the fry and lost income on
the final product. The fish might survive with
minor deformities, but the final fillet will be of
a lower visual quality.
The trials also showed an improvement
in growth performance with a larger share
of the fry reaching the highest size category
at the end of the trial after 140 days, he
added.

Significant changes
in the intestine

While the reduction in deformities and the

improved growth was expected in the Chilean
trial, some new knowledge was obtained
when researchers took a closer look at the
gut histology. They discovered a significant
improvement in the condition of the posterior
part of the gut
The distal part of the intestine of fish fed
18 | INTERNATIONAL AQUAFEED | July-August 2014

feed with added probiotics was significantly

thicker than the same section from fish from
the control group, says Andrs Millan.
He explains that the thickening of the
intestine was the result of an increased length
of the microvilli in the intestine.
This is obviously a very interesting result,
because longer microvilli means improved
nutrient absorption and that again contributes
to a better feed conversion and growth.
The histology also revealed that there
were no signs of inflammation in the intestine
of the fish fed with probiotics.
Andrs Millan believes that this is linked to
the improved micro-flora in the intestine of
fish having received feed with probiotics. But
he also acknowledges, that far more research
has to be done regards to the benefits of
probiotics in fish.
Probiotics have for long been a must
in all husbandry production, and several
probiotics have also been marketed for fish
in Latin America and Asia, but according to
Andrs Millan, this has far too often been
done with very limited and partial documentation.
The effect of probiotics is temperature
dependent, so an indiscriminate transfer of
probiotics which do work at 37 deg C in land
animals to fish living at 8-14 deg C does not
make sense you must carefully select and
document the specific strains which work in
fish at low temperatures.

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July-August 2014 | INTERNATIONAL AQUAFEED | 21

FEATURE

EXPERT TPIC

disease
in the late summer, when temperaIndications
better
underpinned
by thematicof aers,
professionals, techniciansbyand
specialised
tures
peak.
immune
response
on concessions,
production
labour force to respond to the increasing
Jrgen
Holm also underlines that large
BioMar
and the
Canadian
company
d improved health
conditions
demand
by industry
and publicButand
private
, agreed
scale
trials
under
commercial farm conditions by
Lallemand,
who
produces
Bactocell
s action plans aimed at the research programs.
nature
are
difficult
last
year
on
a
longterm
research
collaboration
eases, vaccinations, the use of
Universities and higher education institu- to carry out.
In Denmark
related
to
among
other
the benefits
tions on roe imports.
tions projects
are actively
training human
resourceswe can risk a cold summer with
reduced
of
probiotics.
Some
of
the
first
research
results
on coordinated joint projects oriented towards the satisfaction disease
of thepressure, and then there might
not
be
any
visible differences.
from
this
partnership,
which
in
this
case
also
in the industry to establish industrys requirements in production (marine
However,
this large scale trial also includes the
includes
both
the
University
of
Plymouth
in
the
ures to promote self-regula- biologists, veterinarians, fishing engineers,
utilization
of
diets
United
Kingdom
and
the
University
of
Santiago
ic-private partnership. These aquaculture engineers), processing (industrialwith very different raw material
compositions,
and Jrgen Holm believes this can
de Chile, in
indicate
significant
and consistent
ng existing legislation,
par- a and
food engineers)
and marketing
(commeralso
result
in
interesting
news.
up-regulation
of
genes
involved
in
regulating
eneral Law on Fisheries and cial engineers).
In
Chile
we
saw
significant
positive changes
the
immune
system
in
salmon
between
250g
d adopting new regulations.
There is also a growing specialisation in
in
the
gut
structure,
and
we
have plenty of
and
500g
fed
feed
with
Bactocell.
hrough the effort and dedica- service areas such as environmental impact
documentation
on
an
improved
micro-flora
in the
This
summer
BioMar
is
carrying
out
a
full
ed, recovery is now evident assessment, disease diagnosis and treatment,
is added to the feed.
intestine,
when
Bactocell
season
trial
at
the
Musholm
marine
trial
facility
ry.
biotechnology, market studies and foreign
in Denmark, which will
most among
likely beothers.
the largest
In the Danish
trade,
The Government
has trial we will also study the
single
trial
done
with
probiotics
in
salmonids.
ability
of
the
lactic
a ProChile group which is very helpful in the acid probiotic bacteria to ease
est producer
Jrgen
Holm,
research
and development the digestion process, and we will look deep into
aquaculture industry is cur- trade arena.
manager
in
BioMar
Continental
Europe, expects eventual long term changes in the gut health and
nd largest export sector in
that
the
trial
will
add
important
knowledge
to morphology with and without Bactocell and with
Norway, Chile is the second Annual plan
of action
the
growing
documentation
on
the
effects
of
this
differentmilestone
raw material combinations.
of salmon globally. It has genPerhaps the most important
specific
probiotic
strain.
an 60,000 direct and indirect of the last few years has been the official
We are looking for
both specific
in Aquacultural
A new revolution
in France
es in over 70 markets.
publishing
of thechanges
National
Policy,
the
gut
and
more
general
health
effects.
In France, and
where BioMar recently opened a
e been forged in developing which established objectives, principles
Depending
on thestrategies
disease pressure
in theto
trialthe new
production
and other Latin
American
associated
activitys
sustain-facility for the Larviva hatchery
farm,
we
hope
to
see
a
significant
difference,
when
feed
range,
one of the major hatcheries for
ere is also a push into China able development.
we
look
at
almost
a
full
growth
season
in
the
sea.
Mediterranean
fish species has had tremendous
mand as of now is strong so
This important instrument of public-private
He
bases
his
expectation
on
the
recent
success
with
the
me depth to the marketability participation also established annual plans ofLarviva ProStart range, which is
research results from action
the UK(for
andthe
earlier
results
the 2005),
first hatchery
years
2004 and
whichfeed to include Bactocell.
from
Norway
on
for
example
IPN,
combined
Michel
Autin,
FAO on human resources, have been achieved satisfactorily based on the technical manager at BioMar
with of
theresearchfact that Danish
farms are often hit West Med, tells that by using the Larviva ProStart
quate availability
FAOsea
report.

the French hatchery has

managed to get the rate
of deformities below
one percent and at the
same time it managed
to improve both growth
and survival.
For people who
are acquainted with the
hatchery production for
sea bass and sea bream
Technical Manager Michel
this is a revolution.
Autin and Production &
Logistics Manager Ccile
Ordinary feed products
Soul in in the new
and protocols result in
LARVIVA production facility
deformity rates of typiat the BioMar factory in
cally 15 - 30 percent,
France, examining the
and millions of Euro are
quality of semi finished
LARVIVA Pro products
spent on hand sorting
the total fry production
not once but often twice, to get rid of the deform
fry. With the new French results the hatchery
believes hand sorting is no longer needed and can
take a significant cost saving.
Bactocell was approved in 2009 by the
European Food Safety Authority, EFSA.
It is still today the only probiotic bacteria
strain which is approved for use in fish feed in the
European Union, based on a documented effect
in reducing deformities and improving survival.
BioMar has an exclusive agreement with
Lallemand SAS for the use of Bactocell in fish
feed worldwide..

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