6/8/2014 StudyBlue Flashcard Printing of Rhabdoviridae

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prototype:
vesicular stomatitis
virus
genome -ssRNA
shape: bullet
size: 75x170 nm
enveloped or naked? enveloped.
6/8/2014 StudyBlue Flashcard Printing of Rhabdoviridae
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What two glytoproteins are on
the envelope?
G and M.
_____ nucleocapsid helical
how many proteins
total, what are they?
N, P, M, G, L
Virion associated polymerase is
which protein?
L
circular or linear
genome?
linear
6/8/2014 StudyBlue Flashcard Printing of Rhabdoviridae
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genome size? 11-12 kb
Where is
multiplication?
cytoplasm
Actinomysin D
sensitive or not?
No. It inhibits DNA replication
and cell events, but not the
virus'
What is single cycle yield time?
fast or slow?
6-8 hours, high yield
Viral receptor? gpG
6/8/2014 StudyBlue Flashcard Printing of Rhabdoviridae
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cell receptor? LDL
What kind of
penetration?
receptor mediated
endocytosis
are the endosome pits coated
and if so with what?
clathrin
complete, or partial
uncoating?
partial
acidification:
fusion of envelope with
endosome membrane.
Releases nucleocapsid into
cytoplasm.
6/8/2014 StudyBlue Flashcard Printing of Rhabdoviridae
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-ssRNA has three
functions:
template for +ssRNA to make
5 mRNAs.template to make
+ssRNA for more -
ssRNAprogeny genome
Replication mode is ____
_____ dependent, for what?
protein synthesis, for
N protein
Transcription mode is
catalyzed by what?
virion associated
polymerase
Evidence of enzyme being part
of virus particle?
cyclohexamide inhibits protein synthesis. If
add virion to a cell with cyclohexanmide, still
get enzyme activity, meaning VAP.Cell
polymerase would've been inhibited, but
polymerase had to have come in with virion.
L protein transcribes genome
to make what?
5 +ssRNAs
6/8/2014 StudyBlue Flashcard Printing of Rhabdoviridae
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Evidence for single polymerase
start site on VSV?
UV inactivation study. If irradiate, get
pyrimidine cross-links that stop polymerase
activity. ONLY FOR N did mRNA size =
product size. For others, the target
size>message.N made faster than the others.
Initiation at 3' or 5'
end?
3' end of genome
When making +ssRNA from -
ssRNA, the U7 conserved
sequence causes what?
stutter,
polyadenylation.
GA dinucleotide is
transcribed, or not?
not transcribed. Move
on to P.
Regulation of proteins done through ______
where less enzyme makes it through each
intergenic junction
attenuation. Progressively get
less transcript in molar
amounts of message.
6/8/2014 StudyBlue Flashcard Printing of Rhabdoviridae
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In replication mode,
need what protein?
N
N is what? antiterminator