The sole purpose of this experiment is to see if fungus
can grow on white crust less bread that has been treated with the following substances: apple juice (acid), water (neutral) and liquid hand soap (base). The reason I chose these liquids is because these are items we us everyday for various purposes. For example we drink apple juice because of its vitamins, water is a necessity for life and we use soap to sterilize our hands. Other than the reasons mentioned earlier, my other goal is to see what kind of environment fungi need, or to grow best in. In this experiment I used white bread because white bread will not interfere with the countless colors of fungi. Apple juice has a pH of 3, the water has a pH 0f 7 and the liquid hand soap has a pH of 10.
Hypothesis:
Using my prior knowledge, I can say that molds an only grow on bread that has a neutral substance in it because bases are used to bacteria and molds, while acids may intervene with the mold spores and may react with it.
Materials:
1) 12 white bread slices [crust less (ingredients: white enriched flour, yeast and water)]
2) Concentrate dapple juice [(ACID) ingredients: apple juice concentrate, water and sugar]
3) Liquid hand soap (BASE)
4) Water (NEUTRAL)
5) 12 3X4 reclosable bags
6) 3 teaspoons
7) 12 garage sale labels.
Procedure:
1) Designate each of the teaspoons to the usage of basic, neutral and acidic substances.
2) Put a teaspoon of the basic substance on 4 slices of bread.
3) Do the same as procedure 2 for the neutral and the acidic substances.
4) Number the reclosable bags 1-12.
5) Put the 4 slices of basic bread in the bags numbered 1-4.
6) Do the same thing for the neutral bread and acidic bread. (Put neutral bread in bags numbered 5-8 and put acidic bread in the bags numbered 9-12.
7) Put the 12 garage sale labels on the bags.
8) Write in the labels:
9) Bag number 1: base, warm /light.
10) Bag number 2: base, warm/dark.
11) Bag number 3: base, cool/light.
12) Bag number 4: base, cool/dark.
13) Bag number 5: neutral, warm/light.
14) Bag number 6: neutral, warm/dark
15) Bag number 7: neutral, cool/light.
16) Bag number 8: neutral, cool/dark.
17) Bag number 9: acid, warm/light.
18) Bag number 10: acid, warm/dark
19) Bag number 11: acid, cool/light.
20) Bag number 12: acid, cool/dark.
21) Put the bags to its designated environment and observe.
Data:
WARM/LIGHT WARM/DARK COOL/LIGHT COOL/DARK 1) Fungus grew in small quantities around the piece of bread, except directly on the basic substance
2) Fungus grew, but in very little quantities around the piece of bread. There was an absence of mold on the place where a basic substance was present. The color of the gray, but in some parts it was black.
pH
BASE 3) Fungus completely covered the piece of bread. The color of it is gray, but in some portions its black
4) Fungus grew around the edge of the piece of bread. There was an absence of mold directly on the basic substance. The most visible colors of mold are: gray, black and in extremely small quantities are yellow molds 5) Nearly covered by fungus or molds where the neutral substance (water) was present. The color of the mold was black and gray
NEUTRAL 6) Fungus grew in small amounts around the piece of bread. In the center of the bread is a golden kind of fungus. The most visible colors of fungus are: gray, black, yellow, and golden
7) The piece of bread is almost covered a golden type of molds with patches of black and gray molds.
8) The piece of bread is completely covered by a mixture of golden, black and gray molds. 9) A turquoise type of fungus has completely covered the piece of bread. There is also a strip of yellow fungus
10) A turquoise type of fungus has completely covered the piece of bread. A white kind of fungus is also visible.
ACID 11) A turquoise type of fungus has completely covered the piece of bread. At the bottom of the piece of bread is some golden fungus.
12) A turquoise type of fungus has completely covered the piece of bread. Also, there are some patches a golden fungus.
Conclusion:
After the experiment I now can conclude that fungi (molds) can unanimously grow on neutral bread. It can grow on bread that has been treated with a weak acidic substance. Never-the-less molds cannot grow on basic bread, so it grows around the edge of the bread where the basic substance wasnt present. From this experiment I learned that fungus could basically grow in any type of environment. I could have significantly made this experiment better by carefully controlling the temperature and humidity fluctuations. Other than using only one type of the basic, neutral and acidic substances, I could have used a wide range of them.
Bibliography:
I) Websites: i) Molds. http://www.ecy.wa.gov/programs/wq/ nonprint/ (12 Nov 2004) ii) Mold Guide. http://epa.gov/iaq/molds/moldguide.ht ml (12 Nov 2004) iii) Fungus http://www.franklin.com/present.html (5 Nov 2004) iv) Bkgrnd http://www.geocities.com/~ymjcath/i mg/bkgrnd/ (5 Nov 2004) v) Soyhealth http://www.planthealth.wise.edu/soyh ealth/cause.html (5 Nov 2004) vi) Mold01 http://www.ppdl.purdue.edu/ppdl/wee klypics/-504.html (5 Nov 2004) vii) Slime moulds http://www.ucmp.berkeley.edu/protist a/slimemolds.html (24 Nov 2004) viii) 20Mold http://www.pnw- ag.wsu.edu/smallgrains/Snow%20Mol d.html (24 Nov 2004) ix) Indoor air http://www.health.state.mn.us/divs/eh/ indoorair/mold/ (15 Nov 2004) x) Mold in dry wall http://www.emarresources/photos/mol dondrywall/ (15 Nov 2004)
II) Books: i) Money, Nicholas P. Mr. Bloomfields Orchard. New York: Oxford University Press, 2002. ii) Christensen, Clyde M. The Mold and Man. Minneapolis: University of Minnesota Press, 1975.
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