Professor Choi, Jung Ho TAs: Mcguire, Kathleen, Miles, Brittany BIOL 1510 B, Biology principles 27 September 2013 Laboratory Report 2 Increasing intensity, light and heat do not cause more advantages for Oniscus Asellus to run away better than crawled up into a base. Abstract Different animals have their respective behaviors and living environments. In addition, they would also act differently in certain environments and adapt to them. In this lab, we would like to examine what behaviors and reactions the Oniscus Asellus would have in intensity, light and heat. We have developed our alternate hypothesis. Putting in increasing intensity, light and heat, it will be more advantages for the pillbugs to run away better than crawled up into a base. Pillbugs will eventually run away. In order to find out whether it is true for this alternate hypothesis, we have performed an experiment and obtain possible data. We performed this experiment for three times. Each time, the light source distance to the plate is adjusted to short, mid and long distance. Then, we observed the behaviors of pillbugs and see how they react corresponding to increasing light and heat intensity. Our source of major findings and results mainly come from these three trials of experiment. If our findings show that the pillbugs would run away from increasing light and heat intensity, we would fail to reject our alternate Lam 2
hypothesis. Otherwise, we would reject our alternate hypothesis. We would able to reject or fail to reject our alternate hypothesis after performing T-test and obtain p-value. When performing the T-test, we did not use the data obtained from light source to the plate in mid-distance. Our calculated p-value in this experiment is 0.4019, and it is greater than the alpha value 0.05. Thus, we would reject the alternate hypothesis. As a result, increasing intensity, light and heat do not cause more advantages for pillbugs to run away better than crawled up into a base. This finding reveals pillbugs, Oniscus Asellus, are able to adapt to environment with light and heat intensity. Methods We have designed and followed the procedures during experiments in this lab. We obtained three sets of data by adjusting the light source distance to the plate. The respective distances are 37.9cm, 29.7cm and 19.9cm. The procedures for the experiment are indicated in below. First, setting up the light source and shine to the plate in fixed distance. Since this is the first attempt, we adjusted the distance from light source to the plate to 37.9cm. We created a constant shaded region, and put a piece of paper on the top of the plate. This piece of paper covers less than half size of the plate, and it is fixed by using tape. (Figure A) Then, we measured the radius of the plate, radius of the lit area and radius of shaded region. All distances are measured by using centimeter tape. Figure A:
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Measured Data: 1. radius of the total dish: 18.9 cm 2. radius of lit area: 10 cm 3. radius of the shaded region from center of the dish: 10cm 4. Set up distance on first trial: 37.9cm, second trial: 29.7cm, third trial: 19.9cm
We put ten pillbugs on the plate, and they are covered by a dish. Afterwards, we released the dish, turned on the light source, and turned on the timer until one hundred twenty seconds are reached. During one hundred twenty seconds, we recorded the time instant and number of pillbugs (no.th) comes into shaded region. The data of first trial is obtained, and we repeated the experiment again by adjusting light source distance to the plate with 29.7cm and 19.9cm. Then, we recorded the data of second and third trial. After obtaining three sets of data, we performed T-test and obtained the p-value. However, we did not use the data of second trial to perform T- test. Lam 4
Our calculated p-value in this experiment is 0.4019, and it is greater than the alpha value 0.05. Thus, we would reject the alternate hypothesis. As a result, increasing intensity, light and heat do not cause more advantages for pillbugs to run away better than crawled up into a base.
Analyzing pillbugs for trial 1 (37.9cm)
pillbug number (No.th) the time pillbugs come into shaded region(second, within 120 seconds)
At no.th seconds 1
16 2
17 3
20 4
80 5
119 6 7 8 9 10
Analyzing pillbugs for trial 2 (29.7cm)
pillbug number (No.th) the time pillbugs come into shaded region(second, within 120 seconds) At no.th seconds 1
34 2
37 3
93 4
115 5
116 6 7 8 9 10
Analyzing pillbugs for trial 3 (19.9cm)
pillbug number (No.th) the time pillbugs come into shaded region(second, within 120 seconds) At no.th seconds Lam 5
1
11 2
20 3
32 4
42 5
64 6
97 7 8 9 10
0 20 40 60 80 100 120 140 0 2 4 6 8 10 12 pillbugs come into shaded region (At no. th seconds) pillbug number (No.th) Analyzing pillbugs for trial 1 (37.9cm) Lam 6
calculated p-value for trial 1 and 2: 0.401999 mean value for trial 1 and 2: 47.09091
0 20 40 60 80 100 120 140 0 2 4 6 8 10 12 pillbugs come into shaded region (At no. th seconds) pillbug number (No.th) Analyzing pillbugs for trial 2 (29.7cm) 0 20 40 60 80 100 120 0 2 4 6 8 10 12 pillbugs come into shaded region (At no. th seconds) pillbug number (No.th) Analyzing pillbugs for trial 3 (19.9cm)