Vegasys Service Manual Draft

VEGASYS
Service Manual

Via E. Barsanti 17/A
00012 Guidonia ROME (ITALY)
www.ams-analyzers.com
PRELIMINARY

Vegasys Service Manual Rev.00 08 February 2007 Pagina 1
CONFIGURATION SHEET

CAPITOLO DESCRIZIONE REV.
INDEX TABLE OF CONTENTS 00
01 INTRODUCTION 00
02 GENERAL DESCRIPTION 00
03 INSTALLATION 00
04 ELECTRICAL SCHEMES AND DRAWING 00
05 DIAGNOSTIC PROGRAM 00
06 MAINTANANCE 00
07 SETTINGS AND ADJUSTMENTS 00
08 ERROR SIGNALING 00
09 HOST 00
10 TROUBLESHOOTING 00

INDEX

TABLE OF CONTENTS

Chapter 01 INTRODUCTION
Chapter 02 GENERAL DESCRIPTION
Chapter 03 INSTALLATION
Chapter 04 ELECTRICAL SCHEMES AND DRAWING
Chapter 05 DIAGNOSTIC PROGRAM
Chapter 06 MAINTANANCE
Chapter 07 SETTINGS AND ADJUSTMENTS
Chapter 08 ERROR SIGNALING
Chapter 09 HOST
Chapter 10 TROUBLESHOOTING


Vegasys Service Manual Rev. 00 08 February 2007 Page 1

CHAPTER 01

- INTRODUCTION -

INDEX

1 INTRODUCTION .................................................................................................................... 2
1.1 THE AIM OF THE TECHNICAL MANUAL......................................................................... 2
1.1.2 MANUAL OUTLINE............................................................................................................... 2
1.2 SYSTEM INTRODUCTION.................................................................................................... 4
1.3 PRECAUTIONARY MEASURES .......................................................................................... 5
1.3.1 CHEMICAL RISKS ................................................................................................................. 5
1.3.2 ELECTRICAL RISKS.............................................................................................................. 5
1.3.3 MECHANICAL RISKS............................................................................................................ 5
1.4 GUARANTEE.......................................................................................................................... 6
1.5 TECHNICAL OPERATING FEATURES.............................................................................. 7
1.5.1 COMPUTER & SOFTWARE FEATURE............................................................................... 8
1.5.2 DIMENSIONS, WEIGHT & ENVIRONMENT...................................................................... 9
1.5.3 INSTALLATION REQUIREMENTS...................................................................................... 9



1 INTRODUCTION

1.1 THE AIM OF THE TECHNICAL MANUAL

This manual has been written in order to supply the technical staff, the persons who are responsible
for the maintenance and for resolving instrument failures, a complete and detailed guide of the
Vegasys analyzer, in accordanance with the standard UNI EN591 (which requires a manual to be
supplied with vitro diagnostic instruments for professional use).

1.1.2 MANUAL OUTLINE

The technical manual is composed of 10 chapters that not only describe the operative technical
characteristics of the system, but also the reparation and maintenance procedures to use for the
modules that they are composed of.
. Chapter 1 INTRODUCTION

The manual structure is described, and recommendations are given regarding the general
use of the analyzer. The technical characteristics of the operative system are given in this
chapter.
. Chapter 2 DESCRIPTION OF THE SYSTEM

Describes the system, in particular, the analytical cycle of the single modules and
electronic boards.
. Chapter 3 INSTALLATION

Describes the unpacking procedures and the required characteristics for the place of
installation.
. Chapter 4 ELECTRONICS SCHEMES AND DESIGNS

Gives the electronics schemes and assembly designs of the electronics board and the
assembly designs of the modules that the system is composed of.
. Chapter 5 DIAGNOSTIC PROGRAM

Describes the folders in the Diagnostic Program that are responsible for the checks and the
calibration of the modules in the system.



. Chapter 6 SETTINGS AND ADJUSTMENTS

Gives the procedures for substitution, adjustment and check of the system modules.
. Chapter 7 MANTENANCE

Describes the routine maintenance that needs to be done on a regular basis in order to
guarantee the correct functioning of the analyzer.
. Chapter 8 COMUNICATION WITH HOST

Describes the communication parameters and the ASTM protocol specifics used by the
Vegasys system when connecting to the Host Computer.
. Chapter 9 ERROR SIGNALS GUIDE TO RESOLVE ANOMOLIES

Lists the systems error signals, describes the errors in the results.
. Chapter 10 ANOMALIES GUIDE

Gives a guide for how to resolve anomalies.

The technical staff responsible for resolving the structural anomalies is highly suggested to
thoroughly examine the contents of this manual before operating the system.



1.2 SYSTEM INTRODUCTION

The Liasys system is a continuous-load, random access, bench top instrument for performing
chemical and immuno-turbo-dymetric clinical analysis. It is totally automatic and computer
controlled.

INSERIRE IMMAGINE DEL VEGASYS

Via E. Barsanti 13/A Via E. Barsanti 17/A
00012 Guidonia ROME (ITALY) 00012 Guidonia ROME (ITALY)

Sales & Technical Assistance Manufacturer



1.3 PRECAUTIONARY MEASURES

1.3.1 CHEMICAL RISKS

The individual operator is responsible for assuring that all possible precautionary measures are
taken against eventual risks associated with the use of the Vegasys instrument in clinical
laboratory settings. The manufacturer will provide the reagents kit and specific written information
on the use of each of the reagents.
It is important that the samples be well coagulated and then carefully centrifuged.
Samples which contain fibrinogen clots can obstruct the probe and lead to inexact sampling.
If blood samples containing gel are used, it is suggested that the manufacturers recommendations
be followed.
Immediately clean and remove any accidental leakage of reagent or other liquid.

1.3.2 ELECTRICAL RISKS

As with any electrical device, the risk of electric shock exist.
Is therefore necessary to take every precautionary measure possible when working with this, or any
other, electrical instrument to avoid contact with power supply wires, electrical components or
electronic boards.

1.3.3 MECHANICAL RISKS

Several precautionary measures should be taken when operating the analyzer:
avoid wearing very loose clothing or jewelry that could become tangled in the instruments moving
parts (e.g. the sampling probe); whenever possible, operate the instrument with the main cover
panel lowered.

WARNING: Never attempt to service or substitute any part(s) of the analyzer when the
instrument is turned on.
Any and all technical repairs or servicing must be performed by specialized personnel only.



1.4 GUARANTEE

AMS guarantees the substitution of all defective components and/or materials for a period of time
not above of 14 months starting from the date of invoicing. Saying guarantee, as well as Technical
Assistance, generally is intended furnished as net ex factory Rome.

This guarantee does not include consumable and instrument parts in contact with liquids. All
components not included in the guarantee are reported in the following table.

Besides guarantee does not cover damage caused by :
- improper use of the VEGASYS instrument (or however not according to the Producer or
Seller instructions)
- bad transport
- insufficient (or missing) preventive maintenance by the User

In particular any damages due to the transport must be immediately reported to the carrier when he
delivers.

CONSUMABLES AND ACCESSORIES PARTS LIST OUT OF GUARANTEE
Description Type

Quantity Code



1.5 TECHNICAL OPERATING FEATURES

DESCRIPTION
Open and Fully Automatic, Counter Top, Batch
Analyzer for VET and Clinical Laboratoty. It runs
immunoturbidimetric, clinical chemistry analyses
using single or double reagent methodologies.
ASSAY TYPE
End Point, Initial Rate, Kinetic, Bichromatic, Differential,
TEST ENTRY MODE
Batch, Profiles.
THROUGHPUT
Up to 200 colorimetric test/hr.
WORKING TEMPERATURE
37 C

ON LINE REAGENTS
1 removable reagent try for 18 reagent containers (the
reagent try can be easily removed for refrigeration).
REACTION RCKS
Six racks of 19 reaction cells for a total of 114 cells.
STANDARD & CONTROLS
LOADING
One rack for 6 standard and 2 controls.

SAMPLE LOADING
Four racks for a total of 40 samples.
REACTION VOLUME
Minimum 450 l
Maximum XXX l

SAMPLING & ASPIRATION ARM
The sampling arm contains the sampling probe that
aspirates the reagent and the sample to be analysed and
transfer them into the reaction cell for incubation.
The aspiration arm transfer the reaction mixture from
the reaction cell of the photometric system for
measurement.

DILUTER
Integrated module without syringe with the following
specifications:
Diluter volume: 1000 l
Sample volume: 3.0 l 99 l (1 l incr.)
Precision: CV < 1%
OPTIC SYSTEM & MICRO FLOW
CELL
The photometer system consists of a narrow
bandwidth interference filters of 340 nm, 405 nm,
492 nm, 510 nm, 546 nm, 576 nm, 620 nm.
Light Source: halogen lamp 6V/10 W
Linear range: 0.001 2.5 Abs
Resolution: 0,001 Abs
Micro Flow Cell Volume: 50 l
Temperature: 37 C Peltier effect
Carry Over: 2% nominal for total volume of 450 500
l of solution.


OPTICAL PATH 10 mm.

REACTION PLATE
Six racks of 19 reaction cells for a total of 114
cells.
Incubation temperature: 37C

1.5.1 COMPUTER & SOFTWARE FEATURE
TYPE IBM Compatible
CPU Pentium III 300 MHz, 512 Kb Cache or plus
MEMORY
RAM 256 Mb or plus
Hard Disk 20 Gb or plus
RAM Video: 4 Mb
MONITOR
Colour SVGA 15 low radiation
Resolution 1024 x 768 pixels;
max number of colors 65536 (16 bit)
PRINTER & KEYBOARD
Windows compatible.
Standard (101 Keys) PS2
INTERFACE
Host : RS 232 Serial Port
Instrument Link : RS 232 Serial Port
SOFTWARE
AVAILABLE LANGUAGES
Multitasking WINDOWS XP
Italian, English.
SETTINGS Disable all the energy saving options
Disable the screen saver

NOTE: Even though the computers demonstrate the same technical and operative characteristics,
some of these could have different hardware installed.
This could cause problems for the Vegasys software when running tests (A message appears
indicating Random error or blocks the program).
Therefore, if the PC is bought separately/locally, it is highly recommended to test the system at
your offices before preceding with the installation at the final clients.
Consequently, AMS denies any responsibility for software problems that are due to buying the
computer separately from the instrument.



1.5.2 DIMENSIONS, WEIGHT & ENVIRONMENT

DIMENSION
Height: 32 cm
Depth: 45 cm
Length: 50 cm
WEIGHT 65 Kg
OPERATING ENVIRONMENT Temperature: 18C 32C.
Relative humidity: 20% 85%

1.5.3 INSTALLATION REQUIREMENTS
POWER REQUIREMENTS Input Voltage 110 240 Vac
Input Frequency: 47 63 Hz
Power consumption:
180 W for the analytical unit
600 W for the work station
SAFETY REGULATIONS

ELECTROMAGNETIC
COMPATIBILITY

Warning: A steady power supply (+ 10%) must be provided for the instrument.

If it is not, the manufacturer highly recommends the use of:

UPS Uninterruptible Power Supply (1 KW)
ELECTRONIC STABILISER
Chapter 02 - General Description

Vegasys Service Manual Rev.00 08 Febraury 2007 Page 1

CHAPTER 02 GENERAL DESCRIPTION

INDEX

2.1 SYSTEM DESCRIPTION..................................................................................................2
2.2 MAIN COMPONENTS OF THE ANALYSER.................................................................3
2.2.1 ANALYTICAL PLATE.....................................................................................................3
2.2.2 SAMPLING SYSTEM.......................................................................................................4
2.3 SAMPLING ARM..............................................................................................................4
2.4 ASPIRATION ARM...........................................................................................................4
2.5 PROBE CLEANER............................................................................................................5
2.6 WASHING WELL .............................................................................................................5
2.7 INCUBATOR.....................................................................................................................5
2.8 PERISTALTIC PUMP .......................................................................................................6
2.9 PHOTOMETRIC SYSTEM...............................................................................................7
2.10 MICROFLOW CELL.........................................................................................................8
2.11 COMPUTER & SOFTWARE FEATURE.........................................................................9
2.11.1 TECHNICAL & OPERATIVE FEATURES...................................................................10
2.12 DIMENSION, WEIGHT & ENVIRONMENT...............................................................11
2.13 INSTALLATION REQUIREMENTS ............................................................................11


2.1 SYSTEM DESCRIPTION

The Vegasys is an Open, Fully Automatic Counter Top, Batch Analyzer for VET and Clinical
Laboratory. It runs immunoturbidimetric, clinical Chemistry analysis using sngle or double
reagent methodologies.
The first time the system is used for laboratory analyses, the user must configure the system
based on the specific needs of that laboratory; i.e.: the chemistry parameters and the reagents
rack, along with the normal, calibrated and control values must be defined.
NOTA: In order to assure the analytical performances of the system Instrument + reagents,
it is responsability of the laboratory staff to use reagents, controls, calibrators validated on the
Vegasys, or in alternative, to qualify other reagents, controls and calibrators validated on the
Vegasys, or in alternative, to qualify other reagents, controls and calibrators in compliance
with the applicable relations.

The daily routine analyses will be carried out according to patient sample arrival in a sequential
manner.
The work list is organized using loading rack holding up to 10 patient samples.
In order to access the main program, double click on the Vegasys icon on the computer
desktop.
The main menu System Monitor will appear.


2.2 MAIN COMPONENTS OF THE ANALYSER

2.2.1 ANALYTICAL PLATE

The analytical plate is the main movable part of the analyser. The instrument is equipped with a
18 reagents plate (see Figure 1).

It is provided with:
six racks of 19 reaction cells for a total of 114 cells;
one rack for 6 standards and 2 controls;
four racks for a total of 40 samples;
one removable reagent tray for 18 reagent containers (the reagent tray can be easily
removed for refrigeration).

Figure 1: Analytical Plate


The maximum volume capacity of the containers for the 18 reagents analytical plate are as
follows:

Volume of reaction cell: 1.2 ml
Volume of sample cup: 0.8 ml
Volume of reagent containers : 35 ml
The basic operating cycle takes 18 seconds.
The reactions take place at 37C. This temperature is maintained constant by a controlled
heading unit placed around the reaction plate.

2.2.2 SAMPLING SYSTEM

The sampling system consists of a highly precise diluter controlled by a microprocessor and
has the following specifications:

Diluter volume
1000 l
Sample volume
from 3 to 99 l with 1 l increment
Accuracy
>99%
Materials in contact with the solutions
Teflon & glass
Precision
CV < 1 % a 3 l

The recommended minimum sampling volume for analysis is 5 l and for reagent volume is
400 l. There is also an automatic wash cycle in between tests to avoid the risk of cross-
contamination.

2.3 SAMPLING ARM

The sampling arm contains the sampling probe that aspirates the reagent and the sample to be
analysed and transfers them into the reaction cell for incubation.

2.4 ASPIRATION ARM

The aspiration arm transfers the reaction mixture from the reaction cell to the microflow cell of
the photometric system for measurement.


2.5 PROBE CLEANER

Located at the base of the sampling arm, the probe cleaner consists of a number (approximately
16) of circular filter paper strips mounted on a holder. This cleans the sampling probe before
and after the aspiration and dispensing of reagents.

Figure 2 Sampling Arm

2.6 WASHING WELL

The washing well is the place where the washing of the sampling probe takes place to avoid
cross-contamination between sampling.

2.7 INCUBATOR

The incubation temperature of the reaction cell is fixed at 37C ( 0,2C ) to ensure proper
incubation for all samples.



2.8 PERISTALTIC PUMP

The peristaltic pump operates while the aspiration arm transfers the contents of the reaction
cells into the micro-flow cell.

Pump operation
: by stepper motor
Nominal volume of aspiration
: from 300 to 1000 l
Aspiration rate
: 400 l per 1.5 sec

Figure 3 - Peristaltic Pump



2.9 PHOTOMETRIC SYSTEM

The photometer system consists of a narrow bandwidth interference filters of 340 nm, 405 nm,
492 nm, 510 nm, 546 nm, 576 nm, 620 nm. The other characteristics of the optical system are
as follows:

Absorbance range
0.001 - 2.500 O.D.
Resolution
0.001 O.D.
Drift
< 0.003 O.D./hour
Accuracy
0.001 O.D.
Band-pass
2 nm at 340 & 405 nm; 8 nm for visible range
Light source
6V-10W halogen lamp
Detector
Silicon photodiode
Zeroing
Automatic, computer controlled

Figure 4



2.10 MICROFLOW CELL

The measuring cell of the instrument is a microflow cell with the following characteristics (see
figure 5):

Light path
10 mm
Cuvette body
stainless steel & quartz
Inlet tube
Teflon
Cuvette volume
50 l
Temperature
37C - Peltier effect
Temperature accuracy
0.1C
Carry over
2% nominal for total volume of 450 - 500 l of
solution
Aspiration
computer controlled

Figure 5: Microflow Cell


2.11 COMPUTER & SOFTWARE FEATURE

Minimun requirements:

Type: IBM Compatible.
CPU: Pentium III 300 MHz - 512 Kb Cache or plus.
Memory: RAM 56 Mb or plus.
Hard Disk: 20 Gb or plus.
CD ROM 24X
Memoria Video: 4 Mb
Monitor: SVGA 15 color Resolution: 1024 X 768 pixels; min. number of colors:
65.536 (16 bit)
Rinter: Windows compatible.
Keyboard & Mouse: Standard Keyborad 101 tasti PS2
Interface: RS 232 serial port for the Host Link - RS232 serial port or USB for Vehasys
Link.
Software: Multitasking WINDOWS XP
Settngs: Disable all the energy saving options; Disable the screen saver.

NOTE: Even though the computers dimostrate the same technical and operative characteristics,
some of these could have different hardware installed.
This could cause problems for the Vegasys software when running tests. Therefore, if
the PC is bought separately/locally, it is highly recommended to test the system before
proceeding with the installation at customer laboratories.
Consequently, AMS denies any responsibility for the software problems that are due
to buying the computer separately from the instrument.


2.11.1 TECHNICAL & OPERATIVE FEATURES

Description: Open, Fully Automa. It runs immunoturbidimetric, clinical chemistry
analyses using single or double reagent methodologies.
Assy Type: End Point, Differential, Initial Rate (or Fixed Time), Kinetics and Bichromatis
methods .
Test Selection Mode: Batch, Profile.
Throughput: Up to 2000 colorimetric test/hr.
Working Temperatue: 37 C
On Line Reagents: 18 Reagents (35 ml).
Standards & Controls: one rack for 6 standards and 2 controls.
Samples Loading: four racks for a total of 40 samples.
Minimum Reaction Volume: 450 l
Sampling Arm:provides to aspirate reagent and sample. Automatic probe washing.
Aspiration Arm: transfers the reaction misture from the reaction cell to the microflow
cell of the photometric system of measurament.
Diluter: Integrated module with the following specifications:
Sample volume: 3.0 l 99 l (1 l incr.)
Reagent volume 1: 3.0 l 500 l (1 l incr.)
Reaction Plate: Six sectors of 19 reaction cuvettes for a ttal of 114 cuvettes.
Optic System:
Photometer: multi wavelenght photometer with narrow band
interferntial filters.
Wavelenght: 340 nm, 405 nm, 492 nm, 510 nm, 546 nm, 578 nm and
620 nm.
Light source: halogen lamp 6V/10 W
Linear range: 0.001 2.500 Abs
Resolution: 0.001 Abs
Detector: Silcon photodiode
Optical Path: 10 mm


2.12 DIMENSION, WEIGHT & ENVIRONMENT

Dimension
Height: 32 cm
Depht: 45 cm
Lenght: 50 cm.

Weight: 22 Kg.
Environment:
Temperature: 18C 32 C
Relative humidity: 20% 85%

2.13 INSTALLATION REQUIREMENTS

Power requirements:
Input Voltage: 110 240 Vac
Input Frequency: 47 63 Hz
180 W for the analytical unti
600 W for the work station

WARNING: A steady power supply (10 %) must be provided for the instrument.

If it is not, the manufacturer highly recommends the use of:

UPS (Uniterruptible Power Suppl No Brack module) (1 KW)
ELECTRONIC STABILISER
Chapter 03- INSTALLATION

Vegasys Service Manual Rev.00 08 February 2007 Page 1

CHAPTER 03 - INSTALLATION

INDEX

3.1 UNPACKING..................................................................................................................2
3.2 INSTALLATION............................................................................................................3
3.2.1 INSTALLATION STE SPECIFICATION .....................................................................4
3.2.2 ELECTRIC CURRENT REQUIREMENTS...................................................................4
3.2.3 CONNECTION OF THE ACCESSORIES.....................................................................5
3.2.3.1 POWER SUPPLY...........................................................................................................5
3.2.3.2 LABELS AND SYMBOLS.............................................................................................6
3.2.4 SYMBOLS ......................................................................................................................6
3.2.5 WARRANTY..................................................................................................................7
3.2.6 CONSUMABLES AND ACCESSORIES PARTS LIST OUT OF WARRANTY........7
3.2.7 REGULATORY COMPLIANCE...................................................................................8
3.2.8 LIMITATION OD USE ..................................................................................................8


3.1 UNPACKING

The VEGASYS is packed and delivered in a wooden crate: it contains the analyzer itself along
with its accessories. The packing has been expressly studied and designed to insure maximum
protection of the contents during shipping and handling. It is therefore extremely important that
the crate(s)/box be carefully examined upon delivery in order to ascertain their integrity. Special
attention should be dedicated to examining the color of the Shock Watch glued to the crates,
which must show the color white. A red Shock Watch indicates that the crate(s) have
experienced some sort of shock during handling, transport and/or delivery. This fact must be
noted by the courier on the delivery note, as must any and all visible external damage (for
example: holes, dents, rips or tears, water marks, etc.) evident at the moment of delivery. This
will simplify matters in the event of any future claims for damages.

NOTE: ONCE THE CARRIER HAS TAKEN POSSESSION OF THE SYSTEM FOR
TRANSPORTATION FROM THE FACTORY, THE CARRIER ASSUMES TOTALL
RESPONSABILITY UNTIL ITS DELIVERY. ALL CLAIMS FOR DAMAGE DUE
TO TRANSPORTATION MUST BE FILED AGAINST CARRIER AS SOON AS
THESE DAMAGES ARE NOTICED.

MAKE SURE THAT THE UNPACKING IS CARRIED OUT BY TWO PEOPLE.

Upon arrival of the crate(s)/box, take out the
delivery note and make sure that all the items
on the packing list are included in the crates
and are undamaged. Make sure the series
number on the delivery note/packing list
corresponds to that impressed on the plate on
the right side of the instrument
Open the crate/box from the top and
very carefully take out:
- the instrument and accessories;
- the computer (if required).


Do not discard the delivery crate/box or the packing material until the correct functioning of the
instrument has been ascertained.
Remove all the items from the crate/box very carefully.
Before connecting the "Vegasys", remove the protective packing material of the sampling and
aspiration arms.

Warning: in the event that it is necessary to repack any or all of the delivered item(s), the
following procedures must be carefully followed:

Reposition the protective packing material of the sampling and aspiration arm.
Tape down (using maskig tape if possible) the main cover of the instrument.
Fill the empty spaces around the accessories packed in the crate using pluriballs or other
suitable packing material.

3.2 INSTALLATION

The VEGASYS is an instrument for professional use only and must be installed by a qualified
technician who has been authorised and trained to do so. During its installation the system will
be checked once again to ensure correct functioning. The persons who are required to operate the
LIASYS system must have received the adequate training. This should also include the "know-
how" of the normal maintenance for the instrument. A description of the maintenance will be
found in Chapter 06 of this manual.

VEGASYS is a complex system, and it is therefore extremely important that it is correctly
installed in order to fully guarantee fine performance. If the installation and use directions, given
in this manual, are not correctly followed and/or safety indications are not respected, AMS
cannot guarantee correct functioning of the instrument. Apart from this, the safety of the operator
could be placed at risk.


3.2.1 INSTALLATION STE SPECIFICATION

Ascertain that the VEGASYS system is not exposed to direct sunlight, draughts, dust or strong
magnetic fields. In addition, please take note of the following conditions required for the location
of the installation:

USE In covered and dry place
DEGREE OF POLLUTION 2
INSULATION CLASS I
INSTALLATION CATEGORY II
TEMPERATURE Between 18- 32C
HUMIDITY
20% 85%
ALTITUDE Max 3000 m
LOCATION
Shelf or table with a minimum surface of 70 x 60 cm
stable and free of vibration
VENTILATION
Leave a minimum distance of 10 cm around the
instrument to permit air circulation. Make sure that
the fan (situated under the reagent compartment) is
not blocked by any object

3.2.2 ELECTRIC CURRENT REQUIREMENTS

The power voltages to which the instrument is adapted are indicated on the left-hand side.
It must be plugged into a plug of the correct voltage.

VOLTAGE 100 230 Vac 47/63 Hz 10%
FUSES 5 x 20 4 Amp.

NOTE: IT IS ADVISABLE TO MAINTAIN THE MAXIMUM STABILITY OF THE ELECTRICAL
CURRENT IN THE LABORATORY. WHERE THIS IS NOT POSSIBLE OR ASCERTAINABLE,
USE OF THE FOLLOWING SUPPLEMENTARY DEVICES IS RECOMMENDED:

ELECTRONIC STABILIZER

Used to stabilise the electric voltage in the laboratory. Any stabiliser with a power
potential of at least 1KW, currently available on the market, can be used.


NO-BREAK MODULE UPS - (Uninterrupted Power Supply)

This module provides two important functions:
- stabilises the main-line power
- supplies current to the instrument in case of a main-line power failure.

3.2.3 CONNECTION OF THE ACCESSORIES

3.2.3.1 POWER SUPPLY

IMMAGINE DA CAMBIARE

Fig. 1 Plug (use the feeder cable supplied with the instrument).

3.2.3.2 LABELS AND SYMBOLS

The following label is found at the rear of the instrument.

NOTE: THE PROTECTIONS CARTER OF THE INSTRUMENT MUST NEVER BE OPENED WITHOUT
HAVING FIRST SWITCHED THE INSTRUMENT OFF AND DISCONNECTED THE ELECTRICITY
CABLE.

THE MAINTENANCE AND CLEANING PROCEDURES FOUND IN CHAPTER 07 OF THIS MANUAL MUST
BE RESPECTED AT ALL TIMES. REMEMBER TO FOLLOW THE DECONTAMINATION PROCEDURE
WHEN REQUIRED BEFORE MAINTENANCE OPERATION (SEE CHAPTER 07).

3.2.4 SYMBOLS

ATTENTION: READ THE INSTRUCTIONS IN THE USER MANUAL

TERMINAL OF TOTAL MASS PROTECTION (CONDUCTOR)

BIOLOGIC HAZARD

(LOCATED NEAR THE LIQUID WASTE OUTPUTS)
Carefully manipulate all the consumables and the wastes produced during the
analysis routines. Use appropriate protective garment. Disposal of wastes must
be done in compliance with applicable regulation. It is recommended to
periodically check the level in the waste container, in order to avoid overflow.


3.2.5 WARRANTY

AMS guarantees the substitution of all defective components and/or materials for a period of
time not above of twelve months starting from the date of invoicing. Saying warranty, as well as
Technical Assistance, generally is intended furnished as net ex factory Rome.

This warranty does not include consumable and instrument parts in contact with liquids. All
components not covered by the warranty are reported in the following table.

Besides guarantee does not cover damage caused by:
- improper use of the VEGASYS instrument (or however not according to the Producer or
Seller instructions)
- bad transport
- insufficient (or missing) preventive maintenance by the User

In particular any damages due to the transport must be immediately reported to the carrier when
he delivers.

3.2.6 CONSUMABLES AND ACCESSORIES PARTS LIST OUT OF WARRANTY

Description
Type

Quantity
(Pieces)
Code


3.2.7 REGULATORY COMPLIANCE
The VEGASYS instrument complies with:

European Directive 98/79/CE for In Vitro Diagnostic Devices

3.2.8 LIMITATION OD USE

The VEGASYS can not be used by blind operators because the user interface with the system
requires a monitor.
Furthermore the VEGASYS must be used with particular caution by color blind operators
because the graphic interface displays different colors with different meaning.

Chapter 04 ELECTRICAL SCHEMES AND DRAWING

Vegasys Service Manual Rev. 00 08 Febuary 2007 Page 1

CHAPTER 04

- ELECTRICAL SCHEMES AND DRAWING

INDEX

ELECTRONIC DIAGRAM VEGASYS

HYDRAULIC DIAGRAM VEGASYS

DILUTER ASSY

ANALYTICAL CONTROL BOARD

POWER SUPPLY

CHAPTER 05 DIAGNOSTIC PROGRAM

CHAPTER 05
- DIAGNOSTIC PROGRAM-

INDEX

5 DIAGNOSTIC PROGRAM.............................................................................................................. 3
OPTIONS AREA.................................................................................................................................. 6
FUNCTIONS BAR AREA.................................................................................................................... 6
SETTING ARMS/ PLATE FOLDER................................................................................................... 7
GENERAL ............................................................................................................................................ 9
WASHING WELL .............................................................................................................................. 10
REAGENT .......................................................................................................................................... 11
OFFSET CORRECTIONS ..................................................................................................................... 12
SAMPLE ............................................................................................................................................. 13
CUVETTE UNDER SAMPLING ARM............................................................................................. 14
CUVETTE UNDER THE ASPIRATING ARM ................................................................................ 16
SYSTEM INFORMATION AND PARAMETERS ........................................................................... 18
DILUITOR FOLDER.......................................................................................................................... 19
PRIMING ............................................................................................................................................ 19
ASPIRATION AND DISPENSATION.............................................................................................. 20
DILUTER VALVE TEST................................................................................................................... 21
MICROLITERS RESET ..................................................................................................................... 21
INFORMATION ................................................................................................................................. 21
FOTOMETER TEST FOLDER.......................................................................................................... 22
AUTOZERO........................................................................................................................................ 22
FILTER SELECTION AND START READING .............................................................................. 23
READING FILTERS .......................................................................................................................... 24
DRAWING.......................................................................................................................................... 24
OPTICAL DENSITY Folder............................................................................................................... 25
ASPIRATION ..................................................................................................................................... 25

SELECT FILTER & START READING ........................................................................................... 26
DRAWING.......................................................................................................................................... 27
INFORMATION ................................................................................................................................. 27
PERISTALTIC PUMP FOLDER ....................................................................................................... 28
CALIBRATION.................................................................................................................................. 28
ASPIRATION ANS ASPIRATION STEPS....................................................................................... 29
SOLENOID VALVE TEST................................................................................................................ 29
CANCELING THE PERISTALTIC PUMP STEPS........................................................................... 29
INFORMATION SECTON................................................................................................................. 30
CUVETTE TEMPERATURE............................................................................................................. 30
INFORMATION ................................................................................................................................. 32
PLATE TEMPERATURE................................................................................................................... 32
SEZIONE INFORMAZIONE............................................................................................................. 33
MASCHERA CICLO DI LAVORO................................................................................................... 34
INFORMATION ................................................................................................................................. 38
MASCHERA VARIE.......................................................................................................................... 38
MASCHERA DOWNLOAD .............................................................................................................. 41


5 DIAGNOSTIC PROGRAM
The diagnostic program enables the operator to perform a complete check for each of the Vegasys
module functions.
This program has a folder structure, with each folder containing functions pertaining to the specific
module. To launch the program, the operator has to click with the left side of the mouse, on the
Diagnostic area located on the lower right side of the screen of the System Monitor (Fig.1).
The Liasys must be in the stand-by state to access this area.

Fig. 1

Click on the Diagnostic area, and a window with Password Required will appear. It is necessary
to type in a secret access code and confirm by clicking on OK.

Click Here

This operation can be canceled by clicking on Exit .

After the password is given, the following screen appears: (Fig. 2):

Fig. 2


The following information for the options currently being used is given on this screen:

Icon Symbol for the Language Selected
Master Hardware Version
Serial Port
Firmware Version of the Control Board

The following options are also available:

Closes the screen and returns to the System Monitor.

Gives access to the Main Diagnostic Window.

Enables the operator to select the language (Italian, English, French, German,)

The diagnostic program is subdivided into two distinct areas:

Options
Status Bar


Fig. 3

OPTIONS AREA

FUNCTIONS BAR AREA

The following is information pertaining to each individual function.

Arm/Plate Calibration: Checks if the Sampling/Aspiration Arm and the Reaction
Plate are functioning correctly and performs mechanical
calibration.
Closes the Diagnostic program and returns to the System Monitor.

Diluitor: Checks the performance of the Diluter module;
Photometer Lamp Change: Checks the performance of the Photometer Lamp;
Optic Density: Measures the absorbance of the solution in the Flow Cell;
Peristaltic Pump: To calibrate the aspiration of the Peristaltic Pump;
Temperatura Cuvetta: To calibrate the temperature of the Flow Cell;
Plate Temperature: To calibrate the temperature of the Reaction Plate;
Work Cycle (Work Load): To prepare and/or read one or more samples;
Various: To reset the reaction sectors, print the parameters set for
the various modules and to select a serial port from those
identified by the Master Hardware.
Download: To download and update the Firmware of the Control
Board.

SETTING ARMS/ PLATE FOLDER

Warning: Only qualified technicians are allowed access to the folder Setting Arms/Plate. In
order to access the window it is necessary to insert the Operator Name and Password.


To confirm the information given, click on

The button enables the operator to cancel the operation of accessing the window Arm/Plate
Calibration.

The above window is subdivided into the following area:

General
Washing Well
Reagent
Samples
Cuvette (which is different from the sampling/aspirating arm)
NO3 and Free Chassis
Information and System Parameters


GENERAL

This area has the following commands:

Vertically resets the sampling and aspirating arm
Disengages the motors to enable the Sampling Arm and Reaction
Plate to be manually adjusted.
Note: To engage the motors again just select General Reset.
Performs the resetting of the general system.
Gives information on the screen regarding some functions of the
folder presently open.

WASHING WELL

This area has the following commands:
Brings the sampling arm to the home position (washing well).

Increases the number of steps down of the sampling probe by increments of 1;
Decreases the number of steps down of the sampling probe by increments of 1;

Lowers the sampling probe of a number of steps specified in the small
window under the arrow.
By selecting the command Washing Well, the following buttons
appear automatically which enable the operator to
define the number of steps to go down.
Once the number of steps for the descent are defined, by choosing the
command Down, the sampling probe goes down to the washing well
using the number of steps programmed (in the example, 45 steps).

REAGENT

The following commands are available:


Brings the sampling arm in the reagent position n 1 if
the option is selected (or in reagent position n 10
if the option is selected).
This command lowers the sampling probe down to the reagent container,
using the number of steps indicated in the small window under the arrow.

By selecting this command the following arrows automatically appear
which enable the operator to define the number of
steps down.

Increases the number of steps up of the sampling probe by increments of 1;
Decreases the number of steps up of the sampling probe by increments of 1;
Decreases the number of steps up of the sampling probe by increments of 5;.

OFFSET CORRECTIONS

Moves the sampling arm clockwise 1 step at a time;
Moves the sampling arm clockwise 5 steps at a time;
Moves the sampling arm counter-clockwise 1 step at a time;
Moves the sampling arm counter-clockwise 5 steps at a time.

The same rules apply to the arrows for moving the Reaction Plate with respect to the Sampling Arm.

The command Up makes the sampling probe rise up, using the
number of steps indicated in the small window under the arrow
(in the example, 40 steps).
By positioning the Sampling Arm in the n 1 postion of the reagent, it
is possibile to improve the sampling probes alignment as follows:

Moves the sampling arm (in a clockwise/counter-clockwise
movement) in regards to the reagent container opening.

Moves the reaction plate (in a clockwise/counter-clockwise
movement) in regards to the sampling probe.

This command lowers the sampling probe down to the reagent
container, using the number of steps indicated in the small window
under the arrow.

By selecting this command the following arrows automatically appear
which enable the operator to define the number of
steps down ( in the example, 45 steps).

SAMPLE


Decreases the number of steps down of the sampling probe by increments of 5.

Brings the Sampling Arm to sample cup n 1.
Brings the sampling probe down to the sample cup n 1 using the number
of steps programmed.
By selecting the command Sample, the following arrows
automatically appear which enable the operator to
define the number of steps down.
The Up command brings the sampling probe up using the number
of steps programmed (in the example, 40 steps).
Once the steps down have been programmed, choose the Down
command and the sampling probe will automatically go down to the
sample cup n 1, using the number of steps programmed (in the
example, 45 steps).

Decreases the number of steps up of the sampling probe by increments of 1;
Decreases the number of steps up of the sampling probe by increments of 5.

OFFSET CORRECTIONS



CUVETTE UNDER SAMPLING ARM

The probes alignment can be adjusted when the sampling arm is
positioned in the sample cup n 1 position as follows:
Moves the sampling arm steps (clockwise/counterclockwise)
with respect to the sampling cuvette opening.

Moves the reaction plate n steps (clockwise/counterclockwise)
with respect to the sampling probe.



Decreases the number of steps down of the sampling probe by increments of 5.

OFFSET CORRECTIONS

Allows to bring the first cuvette of the Reaction Plate under the
sampling arm.
Once the steps down have been programmed, choose the Down command; the
sampling probe will automatically go down to the Reaction Plate cuvette n 1, using
the number of steps programmed.

By selecting the command Cuvette-Sampling, the following arrows
automatically appear which enable the operator to define the
number of steps down.
The probes alignment can be adjusted when the sampling arm is
positioned in the reaction cuvettes n 1 position as follows:

Moves the sampling arm (clockwise/counterclockwise) with
respect to the sampling cuvette opening.

Moves the reaction plate (clockwise/counterclockwise) with
respect to the sampling probe.

Once the steps down have been programmed, choose the Down command;
the sampling probe will automatically go down to the reaction cuvette n 1,
using the number of steps programmed (in the example, 45 steps).



CUVETTE UNDER THE ASPIRATING ARM


The first Down command enables the operator to define the first descent of the aspiration probe to
the Reaction Plates cuvette. The depth of the first descent is useful for aspirating liquid for the reaction
(together with the peristaltic pump) which is useful for emptying and rinsing the flow cell. This first
descent depends on the volume which remains in the cuvette of the Reaction Plate which should not be
less than .. (volume which is useful for the next aspiration).

The second Down command enables the operator to define the number of steps down for the second
descent of the aspiration probe into the Reaction Plate cuvette. This second descent should aspirate the
minimum quantity of the reaction liquid necessary for a reading.

Moves the reaction cuvette n 1 under the Aspirating Arm
if option is selected (or the reaction cuvette n 58 if the
option is selected).
1 Down Command 2 Down Command

By selecting the command Cuvette-Aspiration, the following arrows
automatically appear which enable the operator to define the number of steps down.

Increases the number of steps down of the aspiration probe by increments of 1;
Increases the number of steps down of the aspiration probe by increments of 5;
Decreases the number of steps down of the aspiration probe by increments of 1;
Decreases the number of steps down of the aspiration probe by increments of 5.

OFFSET CORRECTION

It is possibile to improve the alignment of the aspiration probes on the
reaction cuvette by doing the following:

Move the reaction plate n steps (clockwise/counterclockwise) with
respect to the aspiration probe.

Moves the Reaction Plate clockwise 1 step at a time;;
Moves the Reaction Plate clockwise 5 steps at a time;;
Moves the Reaction Plate counter-clockwise 1 step at a time;
Moves the Reaction Plate counter-clockwise 5 steps at a time.

SYSTEM INFORMATION AND PARAMETERS

The following tables are available:

TableA

Table B

This table shows the results (under the heading Status) of
various actions which have been run by the
technician/operator in the Diagnostic program.
This table shows all the System settings under the heading
Value.

DILUITOR FOLDER

The above folder is divided into five sections:

Priming
Aspiration and Dispensation
Diluter Valve Test
Zeroing Microliters
Information

PRIMING

This section has the command PRIME and the field Cycles (1..9).


The operations performed during a PRIME cycle are the following:

the diluter aspirates 1000 l of liquid from the loading bottle
the aspirated liquid is dispensed by the sampling probe into the washing well and transferred by
the peristaltic pump to the waste bottle.

ASPIRATION AND DISPENSATION

This section has the following commands and fields:

To aspirate the number of microliters previously programmed in the
field l
To set the number of microliters of liquid that the sampling probe
should aspirate. To conferm the number of microliters
programmed, push Enter (from the keyboard).
To dispense the number of microliters previously aspirated.

Enables the operator to rinse the sampling hydraulic circuit.
To set the number of cycles to be run. (priming)
The minimum number of cycles to be run is 1 and the maximum is 9.

DILUTER VALVE TEST

MICROLITERS RESET

This section shows the number of micro-liters aspirated by the diluter.
By selecting the command Microliters Reset the count of the microliters aspirated are zeroed and
visible in the field Total L.

INFORMATION
This table shows the results (under the heading Status) of various actions which have been run by
the technician/operator in the Diagnostic program.

Allows to test the proper functioning of the diluter valve.
By selecting this command the solenoid valve is automatically turned
ON/OFF.
Shows the hydraulic the sampling circuit.

FOTOMETER TEST FOLDER

The above folder is divided into the following sections:
Autozero
Filter Selection and Start reading
Reading Filter
Drawing
Information

AUTOZERO

Performs the automatic autozero of the fotometer, where the
Vegasys will sample and aspirate distilled water twice (the liquid
located in the loading container). The values read are then
reported on the table and graph (in millivolt and counts) for each
filter.
Reports and Prints the last autozero.

FILTER SELECTION AND START READING

Allows the user to select a filter from a pull down menu list

Allows to start the reading at the filter selected. It turns to STOP when select.

Warning: make sure the micro-flow cell is filled of Rinse solution.

Allows to stop the reading.

By selecting Start Reading the following appears on the screen:

a graph showing the che mostra landamento nel tempo della tensione in mv for the filter
selected, rilevata alla uscita dellamplificatore fotometrico.
A table which shows in mv and Counts for the filter selected:

This section has the field Select Filter and the
command Start Reading.


READING FILTERS

By clicking on the option Filters Reaging, the following information appears on the screen:
una tabella che riporta per ciascun filtro i valori letti in mv (Ultimo) e Counts (Ultimo)
un grafico che mostra in funzione del numero del filtro il valore della tensione in mv rilevata
alla uscita dellamplificatore fotometrico.

DRAWING

Shows the Fotometric System drawing.

INFORMATION

This command automatically performs the reading of the liquid present in
the flow cell for each filter. It is important to make sure that the flow cell
is full of liquid.

OPTICAL DENSITY Folder

This folder is subdivided into two sections:

Aspiration

Select Filter & Start Reading

Drawing

Info

ASPIRATION

This section has the command Aspirationand the
field Aspiration Steps.
Aspirates liquid from the aspiration probe (this is done by
removing the aspiration probe from the aspiration arm and dip it
into the test solution).
This field enables the operator to program the number of steps of the
peristaltic pump in order to improve the aspiration of the liquid.
* Minimum number of steps = 1; Max = 4000

SELECT FILTER & START READING

Allows the user to select a filter from a pull down menu list

Allows to start the reading at the filter selected. It turns to STOP when select.

Warning: make sure the micro-flow cell is filled of test solution.

Allows to stop the reading.

By selecting Start Reading the following appears on the screen:

una tabella che riporta per ciascun filtro i valori letti in mv, Counts e D.O. (densit
ottica)
un grafico che riporta landamento nel tempo della densit ottica.

This section has the field Select Filter and the
command Start Reading.


DRAWING

Shows the Fotometer System drawing.

INFORMATION


Gives information on the screen regarding some functions of the folder
presently open.

PERISTALTIC PUMP FOLDER

This folder is subdivided into five sections:

Calibration
Aspirate and Aspiration Steps
Solenoid Valve
Canceling Peristaltic Pump Steps
Information

CALIBRATION

This section allows to perform an automatic verification and adjustment of the peristaltic pump
aspiration.


ASPIRATION ANS ASPIRATION STEPS

SOLENOID VALVE TEST

CANCELING THE PERISTALTIC PUMP STEPS

This section has two options:
Aspiration and the field Aspiration Steps.
Aspirates liquid away from the aspiration probe.
This field enables the operator to program the number of steps of the
peristaltic pump in order to improve the aspiration of the liquid.
* Minimum number of steps =1; Max= 4000
To test the proper functioning of the diluter valve.
This option automatically switches the solenoid valve ON/OFF.
This section has two options:
Canceling Steps and the field Aspiration Steps.
This option enables the operator to cancel the total number of
Peristatic Pump Steps.
Allows to carry out the calibration procedure.
These fields enables the operator to program the number of aspiration
steps for the peristaltic pump during calibration (Final Aspirat.) and
the number of calibration cycles to run (Cycles (1..9)).

In questa maschera inoltre presente il pulsante che permette di visualizzare lo
schema fotometrico.

INFORMATION SECTON

In questa sezione presenta una tabella che visualizza nel campo Stato le risposte alle azioni
esercitate dal tecnico nelle diverse maschere presenti nel programma di Diagnostica.

CUVETTE TEMPERATURE

Warning: laccesso alla maschera Temperatura Cuvetta permesso al solo personale tecnico
specializzato. Per accedere alla maschera necessario inserire il Nome Operatore e Password.

This field shows the total number of steps run by the
Peristaltic Pump.
La selezione di questo pulsante permette di avere informazioni sul
significato di alcune funzionalit presenti nella maschera in esame.

Per confermare i dati inseriti , selezionare il pulsante

Click on to cancel the operation to access the window Cuvette Temperature.

The above window can be accessed only after a password has been given.
It is possible to perform the calibration procedure of the flow cell using a sonda termica (Kit P/N:
..) as illustrated in the chapter Settings and Calibration.

Starts the calibration process (8 points) of the Flow Cell
Temperature.
Prints the data of the calibration curve and graphically visualizes the
temperature in funzione dei Counts.

INFORMATION


PLATE TEMPERATURE

Attenzione: laccesso alla maschera Temperatura Piatto permesso al solo personale tecnico

Starts the reading process of the flow cells temperature, and updates the
graph as new readings are taken .


Il pulsante consente di annullare loperazione di accesso alla maschera Temperatura Piatto.

La maschera in esame accessibile solo attraverso linserimento di una password.
In questa sezione possibile eseguire la procedura di calibrazione della cella a flusso mediante
lutilizzo di una sonda termica (Kit P/N: ..) come illustrato al capitolo Settaggi e tarature.

SEZIONE INFORMAZIONE

La selezione di questo pulsante permette di avviare la procedura di
calibrazione della temperatura del Piatto di Reazione.
Questo pulsante permette di stampare i dati della curva di calibrazione (8
punti) e di visualizzare il grafico della Temperatura in funzione dei Counts.
Questo pulsante permette di avviare il processo di lettura della
temperatura nel Piatto di Reazione e di visualizzarne landamento grafico
nel tempo.

MASCHERA CICLO DI LAVORO

This window has the option to run one or more test cycles.
This window has the following commands:

Starts the work cycle/s.

This area enables the operator to define the following operations:
Preparation = Prepares the reaction cell with only Regent, or
with Reagent + Sample;
Reading = aspiration and consequent reading of the prepared
mixture in the reading cell
Sample.1 = programs the components of the mixture for the
reading; it is possibile to select a reading of the Reagent alone,
or also include the Sample (only one sample cuvette which
must be located in the first position of the sample secton);
Sample. 1..n = programs the components of the mixture for the
reading; it is possibile to select a reading of the Reagent alone,
or also include the Sample. If this is the case, the number of
cuvettes to be positioned in order in the sampling area starting
with sample n 1, coincides with the number of cycles
programmed.

Note: The Reagent container must be in the first position (R1) of the Reaction Plate.

This areas has the following fields:

N Cycles= to program the number of work cycles;
Vol. Reag. = to define the quantity (l) of the Reagent to be
aspirated
Vol. Camp. = to define the quantity (l) of the Sample to be
aspirated;
Incubation = to program the amount of time for incubation (sec)
of the mixture (Reagent or, Reagent + Sample) in the reaction
cell;
N Readings = to program the number of readings to run at the
end of the incubation period of the mixture in the flow cell;
Min (mAbs) = sets the parameter (in mAbs) for the minimum
value of the reading;
Max. (mAbs) = sets the paramenter (in mAbs) for the maximum
value of the reading;
Section = Indicates the first reaction section ready for use;
Cuvette = Indicates the first reaction cell available for the above
mentioned section. If there is no cuvette available, the symbol ?
appears in the field Section and in the field cuvette, with the
background flashing white and green.
Filter = to select the filter to perform the Reading..

Setting Examples

At the end of the sampling process a table automatically appears, bearing the data from the reading
process and a graph which shows how the reaction progresses over time.
The table has the following information:
N: indicates the cycle number;
D.O: indicates the value of the last reading (espressed in assorbance);
Media: indicates the mean value of the readings (expressed in absorbance);
Min.: indicates the minimum value of the reading (expressed in absorbance);
Max.: indicates the maximum value of the reading (expressed in absorbance)
CV%: indicates the coefficient of the percent of variation;
mv: indicates the millivolt pertaining to the reading;
Pnt/Tmp: indicates the counts pertaining to the NTC and the corresponding temperature in the flow
cell.
During the reading process a a graph automatically appears which shows the progress in time of the
optical density.
If the number of cycles programmed is greater than 1, at the end of the reading process a graph
automatically appears which shows the optical density for every reaction.
The system aspirates Reagent located in the first position of the Reaction
Plate and dispenses in the first available reaction cuvette.
The system aspirates Reagent located in the first position of the Reaction
Plate; at the end of the incubation period the Reagent is aspirated from
the flow cell, and runs the number of readings programmed in the field
N letture.
The system aspirates Reagent + Sample; at the end of the incubation
period the flow cell is aspirated and runs the number of readings
programmed in the field N letture.
The system aspirates Reagent + Sample; at the end of the incubation period
the flow cell in aspirated and runs the number of readings programmed in the
field N letture. The number of Samples examined (trattati) is equal to the
number of cycles programmed in the field NCicli.

INFORMATION


MASCHERA VARIE

This window has the following commands::
Zeroing the reaction sections (SR1, SR2,SR3,SR4,SR5,SR6)
To print the system parameters.
To define a serial port porta seriale to be connect the Vegasys and the PC.

Use this command to visualize the sectors and pertaining reaction cuvettes
used for analysis. (see Table 1).

Tabella 1

To zero a section, click with the mouse on the heading (vedi Fig. 1).
Once a section has been zeroed, the first reaction cuvette in that section becomes automatically
available.

Fig. 1

Important: It is possible to change the status of each single cuvette by clicking on the same.

The reaction cuvettes used for the sample routine of the
Vegasys are indicated in red ( in the example, 6 reaction
cuvettes have been used in section SR1).
The reaction cuvette which have not been used are
indicated in green.

Section Heading

INFORMATION

This field indicates the first reaction cuvette available for sampling
(cuvette n 5).
SR1/C5 = indicates the sector and location available for sampling.

Important: The symbol ? indicates that there are no reaction
cuvettes available for the sampling process.
This option prints the system parameters and saves the results in
the micro (Aspiration Pump, Step Sampling Probe Descent to the
Reagent, Step Sampling Probe Ascent to the Reagent, etc..).
Questo pulsante permette di definire una porta seriale tra quelle
automaticamente rilevate dal Vegasys.

MASCHERA DOWNLOAD

Warning: laccesso alla maschera Temperatura Download permesso al solo personale tecnico


Il pulsante consente di annullare loperazione di accesso alla maschera Download.


This window enables the operator to Download the control boards firmware.

There are two options:

Download with Default parameters to be used for the
first/initial progamming of the Control Board (Firmware???).

Download with automatic saving of the system parameters to be used
for updating the Control Board Firmware. The firmware first reads
the status of the Control Board Firmare parameters, and then at the
end of the Download, transfers once again the previously read
parameters.???? In this manner, the settings made prior to the
updating are saved.
In questa tabella si visualizzano nel campo Stato le
risposte alle azioni esercitate dal tecnico nelle diverse
maschere presenti nel programma di Diagnostica.
Questa tabella visualizza nel campo Valore tutte le
impostazione del sistema.


Chapter 06 - Maintenance


CHAPTER 06 - MAINTENANCE

INDEX

MAINTENANCE ................................................................................................................................ 2
PREVENTIVE MAINTENANCE....................................................................................................... 2
LIST OF PARTS SUBJECT TO WEAR AND USAGE..................................................................... 3
EXTERNAL SAMPLING/ASPIRATION PROBE CLEANING PROCEDURE.............................. 4
WASH SOLUTION BOTTLE - CLEANING..................................................................................... 4
WASH SOLUTION BOTTLE CLEANING PROCEDURE .............................................................. 4
PROBE CLEANER PAD REPLACING PROCEDURE................................................................. 5
HYDRAULIC CIRCUIT WASHING................................................................................................. 6
HYDRAULIC CIRCUIT CLEANING PROCEDURE....................................................................... 6
CHANGING THE PERISTALTIC PUMP TUBE.............................................................................. 7
PROCEDURE FOR REPLACING THE PERISTALTIC PUMP TUBE ........................................... 7
CHANGING THE PHOTOMETER LAMP...................................................................................... 10
PROCEDURE FOR REPLACING THE PHOTOMETER LAMP................................................... 10
CHANGING THE TUBES KIT........................................................................................................ 12
PROCEDURE FOR REPLACING THE TUBES KIT...................................................................... 12
LIST OF THOSE MAINTENANCE PROCEDURES THAT CAN BE PERFORMED BY THE USER AND/OR THE
MAINTENANCA TECHNICAN............................................................................................................... 13
DECONTAMINATION PROCEDURE............................................................................................ 14


MAINTENANCE
This chapter contains all those routine operations, which concern instrument maintenance. Said
procedures, listed and described below, should be carefully and scrupulously followed in order to
guarantee the manufacturers specifications and the perfect working order of the instrument over
time.

PREVENTIVE MAINTENANCE
MAINTENANCE SCHEDULE

Table A, illustrated below, lists all those procedures to be carried out by the user/operator and the
relative frequency schedule. Strict adherence to said schedule will guarantee the optimal operative
efficiency of the instrument.
This program does not include the manual cleaning of reagent containers. Cleaning and decision
about eventual substitution of reagent containers must be made in conformity with good laboratory
practice.

MAINTENANCE SCHEDULE

FREQUENCY PROCEDURE NOTES
DAILY Before launching
Start Work
Check the levels of the wash
solutions.

Start Work
Check the levels of Reagents,
Standards and Controls.

Start Work
Check the levels of the Waste
Bottles and, if necessary, empty it.

Start Work
Check the probe cleaner pad.
DAILY After Shutdown
Clean the Sampling and Aspiration
Probe using either paper toweling or
gauze
See External
Sampling/Aspiration Probe
Cleaning Procedure

FREQUENCY PROCEDURE NOTES
200-300 TEST Change the probe cleaner pad.
See Probe Cleaner Pad
Replacing Procedure
EVERY WEEK
Clean the Sampling and Aspiration
Probe (with sodium hypoclorite).
See External
Sampling/Aspiration
Probe Cleaning
Procedure (see the Note
of the procedure)
EVERY TWO WEEKS Clean the wash solution bottle.
See Wash Solution
Bottle Cleaning
Procedure.
ONCE A MONTH Clean the Hydraulic Circuit
See Hydraulic Circuit
Cleaning Procedure.

EVERY SIX MONTHS Change the Peristaltic Pump Tube
See Procedure For
Replacing the Peristaltic
Pump Tube.
EVERY SIX MONTHS Check all the tubes.
ONCE A YEAR Change all the tubes (Tubes Kit)
See Procedure For
Replacing The Tubes
Kit.
EVERY 2000 HOURS Change the Photometer lamp
See Procedure For
Replacing The
Photometer Lamp.

N. B.: the above-described maintenance schedule refers to that situation in which the workload
of the Analyzer is approximately 500 tests per day. The interval frequency may vary
according to the individual instruments daily workload.

LIST OF PARTS SUBJECT TO WEAR AND USAGE

Description Type Quantity Code
Racks for Reaction Cells
Rack for Sample Cups
(positions 1-10)

(positions 11-20)

(positions 21-30)

(positions 31-40)

Rack for Standard & Control
Cups

Packs of Sample Cups of 0.8
ml

Reagent Containers (XX PCS)
Plastic Bottles (H
2
O/Rinse &
Waste)

Sampling Probe
Aspiration Probe
Tubing Kit Complete
Peristaltic Pump Tube
Probe Cleaner Pad
Photometer Lamp
Kit for E.V.Diluter connection
Diluter E.V.
Peristaltic Pump E.V.
Wash Solution
Diluter Head Teflon Fitting
Diluter Fitting


EXTERNAL SAMPLING/ASPIRATION PROBE CLEANING PROCEDURE

1) Turn off the Analyzer.

2) Loosen the knob which blocks the sampling/aspiration probe to the sampling/aspiration arm.

3) Remove the sampling/aspiration probe from the support frame.

4) Use only lint-free paper toweling or gauze.

5) Dampen the gauze or paper toweling with distilled water and clean outside of the
sampling/aspiration probe. Wipe the probe from the top downwards only! This is to avoid
that any bits of cloth, paper or lint fibers accidentally enter the probe itself.

6) Insert sampling/aspiration probe in the support frame of the sampling/aspiration arm.

7) Tighten the knob which blocks the sampling/aspiration probe in the sampling/aspiration arm.

Note: The manufacturer suggest that once weekly the above described cleaning procedure be
performed on a weekly basis using, instead of only simple distilled water, a 5% sodium
hypochlorite solution to dampen the gauze and then be repeated using distilled water.

WASH SOLUTION BOTTLE - CLEANING

During normal use and over time, mold and dust can build up inside the wash solution bottles.
For this reason, it is extremely important that they be periodically washed. This cleaning must be
thorough and meticulous in order to insure that every trace of mold or residue be removed.

How often the bottle must be cleaned depends on their use and on the quality of the distilled water
used in that particular laboratory. However, the manufacturer recommends thorough washing at
least once every two weeks.

It is extremely important that the user not underestimate the risks associated with mold and dust
particles. They are to be regarded as a serious hazard as they can be the cause of instrument
malfunction.

WASH SOLUTION BOTTLE CLEANING PROCEDURE

1) Turn off the Analyzer;

2) Pull the aspiration cannula out from the bottle;

3) Take the cap off the bottle and empty it;

4) Fill the bottle with 5 % sodium hypochlorite solution;

5) Clean the inside of each bottle using a bottlebrush in order to remove all traces of mold
and/or residue;


6) Leave the sodium hypochlorite solution stand in the bottles for at least ten minutes;

7) Empty the bottle, rinse it repeatedly and well with tap water, and then twice more using
distilled water;

8) Dry the bottle;

9) Fill the bottle with proper solution (Rinse);

10) Close the bottle;

11) Reconnect the aspiration cannula;

12) Carry out eight PRIME cycles checking that the sampling hydraulic circuit is filled of
liquid.

PROBE CLEANER PAD REPLACING PROCEDURE

Figure 1

Located at the base of the sampling arm, the probe cleaner consists of a number (approximately 16)
of circular paper strips mounted on a holder. This cleans the sampling probe before and after the
aspiration and dispensing of reagents.

1. Turn off the Analyzer;
2. Remove the probe cleaner pad from its housing (located at the base of the sampling arm);
3. Replace the used probe cleaner pad with a new one;



HYDRAULIC CIRCUIT WASHING

During normal use and over time, mold and dust can build up inside the wash bottle and can have a
negative effect on the hydraulic circuit, compromising the correct functioning of the system.
For this reason, it is extremely important that the hydraulic circuit be periodically washed. Said
cleaning must be thorough and meticulous in order to assure that every trace of mold or residue be
removed.

How often the hydraulic circuit must be washed depends on the operating conditions/environment
of the single instrument and the quality of the distilled water used in that particular laboratory. The
manufacturer recommends thorough washing at least once a month.

It is extremely important that the user not underestimate the risks associated with mold and dust
particles. They are to be regarded as a serious hazard as they can be the cause of instrument
malfunction.

The hydraulic circuit input cannula is located inside the wash solution bottle.

HYDRAULIC CIRCUIT CLEANING PROCEDURE

1) Turn on the Analyzer;

2) Prepare a bottle containing 500 ml of a 5% sodium hypochlorite solution;

3) Insert the aspiration cannula, located inside the wash solution bottle, into the bottle containing the
sodium hypochlorite solution;

4) Have the instrument carry out eight PRIME cycles;

5) Have the instrument carry out three Autozero cycles;

6) Pull the aspiration cannula out from the bottle containing the sodium hypochlorite solution;

7) Clean and dry the aspiration cannula. Insert the aspiration cannula into a bottle containing
distilled water;

8) Have the instrument carry out five PRIME cycles;

9) Have the instrument carry out three Autozero cycles;

10) Insert the aspiration cannula back into the respective bottle (wash solution bottle). Said
bottle should have, in the meantime, been cleaned and filled with a fresh supply of the
required solution;

11) Have the instrument carry out a PRIME cycle and then an Autozero cycle to check the
system efficiency.



CHANGING THE PERISTALTIC PUMP TUBE

The manufacturer recommends that the Peristaltic Pump tube be replaced every six months. Said
frequency may vary depending on the workload of the individual laboratory. The quality and
reliability of these tubes is fundamental to a correct emptying of the micro-flow cell.

PROCEDURE FOR REPLACING THE PERISTALTIC PUMP TUBE
The diameter and the length of the tubings for the flow system are very critical for the proper
functioning of Vegasys. Please make sure that the tubings are in accordance with the manufacturer's
specifications.

1. Switch off the instrument and unplug the power cord;
2. Loosen knob A and move block B downwards (see Figure 2);
3. Remove the old tube from the connection;
4. Replace the new tube and connect it again to the junction;
5. Push block B up to block D and tighten knob A;
6. Check the aspiration volume using the "Start Calibration" function of the Vegasys
software (Chapter 06 Calibration Pump Procedure).
7. Have the instrument carry out an Autozero cycle as functional check.

Figure 2



CALIBRATION PUMP PROCEDURE

This procedure enables the user to set up the volume of the reaction mixture that is aspirated by the
peristaltic pump from the reaction cell to the microflow cell via the aspiration tube.

Warning: before activating this procedure, one reagent container with Rinse Solution must be
placed on the analytical plate at reagent position R1.

1. Launch Diagnostic program and wait for an instrument general reset.;
2. In order to view the various elements contained under Diagnostic program, insert the
valid Password within the provided field. Click on OK;
3. Press Peristaltic Pump button;
4. Press Start Calibration button; the instrument carry out a microflow cell wash cycle;
5. Make sure that the tubes (leading to and from the microflow cell) fill up of liquid, as shown
in the below figure (see the bold black lines).

Figure 3

(A) (B)


6. Referring to the diagram above, where the solid bold, black lines indicate the silicon tubes
leading to and from the microflow cell. If in the area B the liquid goes too much beyond the
bold area indicated meaning that there is too much liquid in area B, and if in area A the
liquid does not completely fill the bold, black line, it is necessary to decrease the steps in the
Final Aspiration field. Push the Start Calibration button again and check the liquid
level in the tubes. On the other hand, if the liquid in area A is too much and in area B there
is not enough, increase the number of steps in the Final Aspiration field. Push the Start
Calibration button again and check the liquid level in the tubes.

Nota: If the adjustment cannot be made by adjusting the software, it is necessary to adjust the
pressure between the silicon tube and the peristaltic pump wheel through the keep plate of the
peristaltic pump.

WASHING MICRO FLOW CELL - PROCEDURE

This procedure enables the automatic washing of the microflow cell with a 5% sodium hypochlorite
solution. Before activating this procedure, put some 5% sodium hypochlorite solution into one of
the reagent container.

1) Put some 5% sodium hypochlorite solution into one of the reagent container;
2) Place the reagent container with 5% sodium hypochlorite solution in position R1 of the
Reaction Plate;.
3) Ensure that the reaction rack SR1 is available in the analytical plate;
4) Launch the Diagnostic program;
5) In order to view the various elements contained under Diagnostic program, insert the
valid Password within the provided field. Click on OK; wait for the instrument general
reset;
6) Select Diagnostic button;
7) Select Work Cycle button;
8) Press Start Cycle; the sampling probe takes the sodium hypochlorite solution from the
reagent container and dispense it into the first free reaction cell. The aspiration probe will
then take the solution in the reaction cell and the solution flows through the microflow cell;
9) Leave the sodium hypochlorite solution stand in the microflow cell for at least 15 minutes;
10) Remove the reagent container with sodium hypochlorite solution from R1 position of the
Reaction Plate;



11) Place a reagent container with Distilled Water in position R1 of the Reaction Plate;
12) Set the N Cycles equal to 5;
13) Press Start Cycle.

The manufacturer suggests to carry out the below-described washing microflow cell
procedure al leas once per week.

CHANGING THE PHOTOMETER LAMP

The manufacturer suggests that the lamp be replaced after approximately 2000 hours of use.
The lamp is mounted on the photometer, which is located on the left-hand side of the reaction plate.

PROCEDURE FOR REPLACING THE PHOTOMETER LAMP

WARNING! DO NOT TOUCH THE GLASS PART OF THE LAMP WITH YOUR FINGERS!
IF NECESSARY, USE A CLEAN CLOTH TO REMOVE DUST, OR ALCOHOL TO REMOVE MORE
STUBBORN DIRT.

Figure 4

2. Unscrew the screw which holds the white cover panel of the photometer. Remove the
white cover panel.
3. Unthread the terminals of lamp. (designated as "A" in Figure 4).
4. Unscrew the block of lamp. (designated as "B" in Figure 4) and remove the lamp;


5. Replace the old lamp with a new one making sure that the pin is correctly inserted in the
alignment hole;
6. Make sure that the lamp regulator voltage is 6.0 Volt 0.1; if not, turn the TR1
trimmer located on the Vegasys Control Board.;
7. Remount the assembly by repeating the above step 5 through 1 in reverse order;
8. Turn the Analyzer on;
9. Launch Diagnosticprogram;
11. Select Diagnostic button and then, Change Photometer Lamp;
12. Make sure that the wash solution bottle is filled of proper and fresh solution;
13. Select Autozerobutton;

The Vegasys will automatically sample and aspirate wash solution twice, performing the autozero
and displaying a chart which states the read value expressed in mv and Counts for each filter.

Acceptable values (in mv and counts) are given below (see Table C and D):

Table C

Filter Min. Value (mv) Max. Value (mv)
Dark
340, 405, 492, 510,
546, 578 e 620

Tabella D

Filter Min.Value (Count) Max. Value (Count)
Dark
340, 405, 492, 510,
546, 578 e 620

If the 340 filter and/o other filters do not present the correct value, before following the set-up
procedure for Gain and Offset, check if:

microflow cell is perfectly clean:
microflow cell is full of liquid and that there are no air bubbles on the inside.



GAIN & OFFSET - ADJUSTMENT

Warning: room lighting and daylight can influence the reading

1. Make sure that the Dark value shows a count value of XY0 Z0; if not, adjust the
Preamp/ADC board Offset trimmer in order to set the desiders value;
2. Identify which filter transmit the highest signal;
3. Verify for the filter that transmit the highest signal a count value of ? ?; if not, adjust the
Preamp/ADC board Gain trimmer in order to set the desired value;
4. Press Filters Reading;
5. Verify for all the filters (from 340 nm to 620 nm) count values between ?? and ??;
6. Exit from Diagnostic program.

CHANGING THE TUBES KIT
Over time and through normal use, the tubes become worn.
The manufacturer suggests that the tubes be replaced at least once a year.

PROCEDURE FOR REPLACING THE TUBES KIT

2. Unlock the four screws of the instrument chassies (make a quarter of turn for each screw);
3. Line up the sampling probe arm to the center of the Reaction Plate;
4. Remove the instrument chassies;
5. Replace the tubes following the indication shown on the Hydraulic Tubing Diagram;
6. Turn on the Analyzer;
7. Launch the Vegasys software;
8. Launch Diagnosticprogram;
10. Select Diluter button;
11. Carry out eight PRIME cycles to make sure that the sampling hydraulic circuit is filled of
liquid;
12. Select Change Photometer Lamp button;
13. Select Autozero button to make sure that the aspiration hydraulic circuit is filled of liquid;
14. Line up the sampling arm to the center of the Reaction Plate;



15. Remount the instrument chassies and lock the four screws (make a quarter of turn for each
screw);
16. Carry out three PRIME cycles to make sure the sampling hydraulic circuit is filled of
liquid;
17. Carry out two Autozero cycles to check the system efficiency. If needed, carry out the
Calibration Pump Procedure.

LIST OF THOSE MAINTENANCE PROCEDURES THAT CAN BE PERFORMED BY THE USER AND/OR
THE MAINTENANCA TECHNICAN

PROCEDURE

FREQUENCY (*)

EFFECTUATED BY

CLEANING THE SAMPLING/ASPIRATION PROBE

DAILY

OPERATOR

CLEANING THE WASH SOLUTION BOTTLE

EVERY TWO WEEKS

OPERATOR

REPLACING THE PROBE CLEANER PAD

200 300 TEST

OPERATOR

REPLACING THE PERISTALTIC PUMP TUBE

EVERY SIX MONTHS

OPERATOR

REPLACING THE PHOTOMETER BULB

AFTER 2000 HOURS
OF USE

OPERATOR

WASHING MICROFLOW CELL WEEKLY OPERATOR

REPLACING THE SAMPLING/ASPIRATION PROBE

AS NEEDED

OPERATOR

CLEANING OF THE HYDRAULIC CIRCUIT MONTHLY OPERATOR

REPLACING THE TUBES (Tubes Kit)

ONCE A YEAR

MAINTENANCE TECH

REPLACING THE BELT AS NEEDED

MAINTENANCE TECH


REPLACING A MOTOR AS NEEDED

MAINTENANCE TECH

ADJUSTING PHOTOMETER AS NEEDED

MAINTENANCE TECH

REPLACING THE ELECTRONIC BOARDS AND
THE MECHANICAL MODULES
AS NEEDED

MAINTENANCE TECH

N.B.: The above indicated frequency intervals may vary according to the individual
instruments daily workload.

DECONTAMINATION PROCEDURE

Before replacing any instrument parts, repairing any defective items or performing any instrument
maintenance procedure(s), the operator or maintenance technician must carry out the below-
described decontamination procedure of the instrument part(s) involved in the operation(s).
This procedure can be performed on:
the entire Analyzer

those part(s) of the instrument subject to possible contamination

NECESSARY MATERIAL

an ESOFENOL solution diluted to 6% (60 cc in one liter of distilled water).
ESOFENOL is an antibacterial and antiviral substance.

Rubber gloves

Mask

Lab coat

EXTERNAL SURFACES AND INDIVIDUAL PARTS

Spray the solution all over the instrument, paying particular attention to wetting:

- the sampling arm
- the reaction plate (including the cuvettes)
- the racks
- the instrument chassis
-
Allow the solution to stand for approximately 30 minutes

Wipe the solution off the instrument and the various components using a sponge
dampenedwith distilled water


Carry out a decontamination of the internal hydraulic circuit.

HYDRAULIC CIRCUIT (ENTIRE INSTRUMENT)

Fill a container with a 5% sodium hypochlorite solution;

Insert the aspiration cannula, located into the wash solution bottle, into a bottle filled with
the 5% sodium hypochlorite solution and have the instrument carry out three Autozero
cycles;

Remove the aspiration cannula from the 5% sodium hypochlorite solution and immerge it in
another bottle containing Rinse Solution. or Distilled Water;

Have the instrument carry out three Autozero cycles;

Insert the aspiration cannula into its proper bottle.



CHAPTER 07
- SETTINGS AND ADJUSTMENTS -

INDEX

SAMPLING AND ASPIRATION ARMS .......................................................................................... 2
DILUTER MODULE .......................................................................................................................... 9
PHOTOMETER MODULE............................................................................................................... 11
PERISTALTIC PUMP MODULE..................................................................................................... 16



SAMPLING AND ASPIRATION ARMS

Changing the Sampling Arm

1. Turn off the Vegasys analyzer;
2. Remove the front casing/chassis, unscrew the 4 holding screws and remove the side
casings/chassis;
3. Remove the sampling probe, first loosening the knurled knob;
4. Unscrew and remove the screw;
5. Disconnect the cable;
6. Remove the old arm;
7. Insert the new arm, making sure that it is positioned as far down as possible;
8. Reconnect the cable;
9. Put the screw back in, but do not over-tighten;
10. Put the side casings/chassis back on;
11. Put the sampling probe back in position;
12. Replace the front casing/chassis;
13. Put the screw back in but do not over-tighten the knurled knob, in order to allow the
sampling probe to move back up if necessary;
14. Turn the Vegasys analyzer back on;
15. Launch the Diagnostic programme;
16. Select Arms/Plate Setting and wait until the system has carried out an instrument reset;
17. Position the sampling arm over the wash well and tighten it;
18. Carry out a General Reset;
19. Make sure the sampling probe is balanced and centred over the wash well;
20. Tighten the sampling arm and repeat the General Reset.
21. Make sure that the sampling probe is still correctly balanced and centred over the wash well
(if not, loosen the screws holding the sampling arm in place and repeat steps 19 and 20);
22. Tighten the knurled knob screw;
23. Check the downward movement of the sampling probe using the number of preset steps; the
optimal downward movement is when the final distance between the top lip of the wash well
and the tip of the sampling probe is at least 3 cm (if necessary, correct the number of preset
steps);



24. Refer to procedure 6.1.1 CENTRING AND ADJUSTMENTS from step #11 to step # 25;
25. Exit the Diagnostic programme.

Replacing the Aspiration Arm

1. Turn off the Vegasys analyzer;
2. Remove the front casing/chassis, unscrew the 4 holding screws and remove the side
casings/chassis;
3. Remove the aspiration probe, first loosening the knurled knob;
4. Unscrew and remove the screw;
5. Disconnect the cable;
6. Remove the old arm;
7. Insert the new arm, making sure that it is positioned as far down as possible;
8. Reconnect the cable;
9. Put the screw back in, but do not over-tighten;
10. Put the side casings/chassis back on;
11. Put the aspiration probe back in position;
12. Replace the front casing/chassis;
13. Put the screw back in but do not over-tighten the knurled knob, in order to allow the
sampling probe to move back up if necessary;
14. Turn the Vegasys analyzer back on;
16. Select Arms/Plate Setting and wait until the system has carried out an instrument reset;
17. Manually position the aspiration arm over the cuvette in the Reaction Plate making sure that
it is perfectly centred;
18. Tighten the aspiration arm;
19. Carry out a General Reset;
20. Check that the aspiration probe is perfectly centred and balanced over the Reaction Plate
cuvette;
21. Tighten the aspiration arm and repeat a General Reset;
22. Check the downward movement of the aspiration probe using the number of preset steps
programmed for the first dip of the aspiration probe into the Reaction Plate cuvette;



23. Make sure that the aspiration probe is still correctly positioned (if not, adjust the positioning
of the aspiration probe, centring it with respect to the reaction cuvette opening by using the
shift buttons below the GO Cuvette Aspiration button (green
buttons used to adjust the position of the Reaction Plate with respect to the Aspiration Arm).
24. Select Vertical Reset;
25. Regulate the descent of the aspiration probe using the first Down button making sure that
the distance between the bottom of the Reaction Plate cuvette and the tip of the aspiration
probe is approximately 27 mm.
If the distance measured is not acceptable, use the shift buttons (yellow
buttons) placed below the first Down button to change the number of steps;
N.B.: this initial calibration of the descent of the aspiration probe into the reaction cuvette is
carried out with the purpose to insure that the flow cell is correctly primed;
26. Carry out a second setting of the descent of the aspiration probe using the second Down
button, making sure that the distance between the bottom of the Reaction cuvette and the tip
of the aspiration probe is approximately 1 mm.
buttons) placed below the second Down button to change the number of steps;

N. B.: this second calibration of the descent of the aspiration probe into the reaction cuvette is
carried out with the purpose to insure that all the liquid is aspirated and which, in any case, must not
be less than xx l.

27. Tighten the knurled knob;
28. Perform a Vertical Reset



Adjusting the Aspiration and Sampling Arms

1. Turn on the Vegasys system and launch the instrument management programme by double
clicking on the Vegasys icon;
2. Select Diagnostic;
3. Insert the required password in the Password Request window and then confirm by
clicking on OK;
4. Select Diagnostic in the mask that opens and wait until the instrument has carried out the
Reset function;
5. Click on Arms/Plate Setting;
6. Enter the User Name and Password in the window that opens and confirm by clicking
on OK;
7. Make sure that the sampling probe is properly centred with respect to the wash well; if not,
loosen the arms holding screw and mechanically align it over the wash well so that it is
properly centred;

INSERIRE FIGURA

8. Tighten the sampling arms holding screw;
9. Carry out a General Reset and once again check that the probe is properly centred over the
wash well. If not, repeat steps 7 and 8;
After having mechanically centred the sampling probe over the wash well, regulate all the
other positions using the buttons included in the mask, as described in the following
instructions:
10. Select Wash Well; regulate the descent of the sampling probe into the wash well using the
Down button making sure that the distance between the top lip of the wash well and the tip
of the probe is at least 3 cm. If the distance measured is not acceptable, use the shift buttons
(yellow buttons) to change the number of steps;
11. Select the GO Reagent button; the sampling probe will automatically position itself over
the reagent in position # 1;
12. Regulate the descent of the sampling probe using the Down button, making sure that the
distance between the bottom of the reagent container and the tip of the sampling probe is 1.2
mm 0.2 mm .



buttons) to change the number of steps;
13. If necessary, regulate the centring of the sampling probe with respect to the reagent container
opening by using the buttons placed above and below the GO Reagent button;
(pink shift buttons used to adjust the position of the Sampling
Arm with respect to the Reaction Plate)
(green shift buttons used to adjust the position of the Reaction Plate
with respect to the Sampling Arm);
14. Regulate the upward movement of the sampling probe by using the Up button, making sure
that the tip of the sampling probe comes out of the cleaning pad by 1 mm.
If necessary, regulate this distance by using the violet coloured shift buttons
to change the number of steps;
15. Under the GO Reagent button, select option 10.
16. Click on GO Reagent, the sampling probe will automatically place itself in position # 10;
17. Regulate the descent of the sampling probe by using the Down button, making sure that the
distance between the bottom of the reagent container and the tip of the sampling probe is 1.2
mm 0.2 mm.
18. If necessary, regulate the centring of the sampling probe with respect to the reagent container
opening by using the buttons placed above and below the GO Reagent button;
(pink buttons used to adjust the position of the Sampling Arm
with respect to the Reaction Plate)
(green buttons used to adjust the position of the Reaction Plate with
respect to the Sampling Arm);
19. Select the GO Sample button, the sampling probe will automatically position itself over the
first sample in the first samples rack;



distance between the bottom of the sample cup and the tip of the sampling probe is 1.2 mm
0.2 mm.
21. If necessary, regulate the centring of the sampling probe with respect to the sample cup
opening by using the buttons placed above and below the GO Sample button;
(pink shift buttons used to adjust the position of the Sampling
Arm with respect to the Reaction Plate)
(green shift buttons used to adjust the position of the Reaction
Plate with respect to the Sampling Arm);
22. Regulate the upward movement of the sampling probe by using the Up button, making sure
that the tip of the sampling probe comes out of the cleaning pad by 1 mm.
If necessary, regulate this distance by using the violet coloured shift buttons
to change the number of steps;
23. Select the GO Cuvette - Sampling button, the sampling probe will automatically position
itself over the first cuvette of the first reaction sector;
distance between the bottom of the reaction cuvette and the tip of the sampling probe is 38
mm.
25. If necessary, regulate the centring of the sampling probe with respect to the reaction cuvette
opening by using the buttons placed above and below the GO Cuvette Sampling button:
(pink buttons used to adjust the position of the Sampling Arm
with respect to the Reaction Plate)
(green buttons used to adjust the position of the Reaction Plate
with respect to the Sampling Arm).



N. B.: the following steps have to do with regulating the aspiration arm in the reaction cuvette.
Before allowing the aspiration probe to dip into the reaction cuvette, make sure that the aspiration
arm is perfectly aligned with the reaction cuvette. This is absolutely necessary in order to ensure
that the aspiration probe does not come in direct contact with or rub the lip, sides or edges of the
reaction cuvette.

26. Select the GO Cuvette Aspiration button; the first cuvette of the first reaction sector will
automatically position itself directly under the aspiration probe;
27. Regulate the descent of the aspiration probe by using the first Down button, making sure
that the distance between the bottom of the Reaction Plate cuvette and the tip of the aspiration
probe is approximately 27 mm.
buttons) placed below the first Down button to change the number of steps;
N.B.: this initial calibration of the descent of the aspiration probe into the reaction cuvette is
carried out with the purpose to insure that the flow cell is correctly primed.
If necessary, regulate the centring of the aspiration probe with respect to the reaction cuvette
opening by using the buttons placed below the GO Cuvette - Aspiration button:
(green buttons used to adjust the position of the Reaction Plate with
respect to the Aspiration Arm;
28. Carry out a second setting of the descent of the aspiration probe by using the second Down
button, making sure that the distance between the bottom of the reaction cuvette and the tip of
the aspiration probe is approximately 1 mm.
buttons) placed below the second Down button to change the number of steps;

N. B.: this second calibration of the descent of the aspiration probe into the reaction cuvette is
carried out with the purpose to ensure that all the liquid is aspirated said liquid must, in any
case, not be less than xx l.

Changing the Sampling Arm Motor



DILUTER MODULE

Changing the diluter module

N.B.: The instrument (Vegasys) must be turned off during the execution of the following
procedure.

1. Loosen the four screws that hold the instrument casing/chassis in place (a quarter turn each);
2. Align the sampling arm directly over the centre of the Analysis Plate (this makes it possible to
remove the instrument casing/chassis);
3. Remove the instrument casing/chassis;
4. Disconnect the J? and J?? connectors from the control board;
5. Remove the two Teflon tubes positioned along the side of the diluter by unscrewing their
clamps. Be careful of leakage;
6. Unscrew the two screws on the base that supports the diluter (Fig. X);
7. Unscrew the four screws that fasten the dilutor block to its support base;
8. To remount the diluter, repeat the above four steps in inverse order or rather: perform steps
7, 6, 5, and 4 in this order.
9. After remounting, carry out the mechanical functional check as described in section XX

Replacing the diluter electro-valve



procedure.

2. Align the sampling arm directly over the centre of the Analysis Plate (this makes it possible to
remove the instrument casing/chassis);
4. Disconnect the J? connector from the control board;
5. Remove the Teflon tubes from their respective housings in the electro-valve;
6. Unscrew the two screws that fasten the diluter, take it out and put in its replacement;
7. To remount, repeat the above six steps in inverse order or rather: perform steps 6, 5, 4, 3, 2
and 1 in this order;
8. After remounting, carry out the mechanical functional check as described in section XX

Mechanical functional check of the diluter

1. Launch the Diagnostic programme from System Monitor;
2. Select the Diagnostic button and wait until the instrument has carried out a reset;
3. Select Arms/Plate Setting ;
4. Enter the user name and password in the Enter User Name and Password window;
5. Select the Free Casing/Chassis button;
Wait until the sampling arm has positioned itself directly over the centre of the Reaction Plate;
8. Select the General Rest button and wait until the arm has positioned itself over the wash
well;
9. Select Dilutor;
10. Select first the aspiration and then the dispense buttons for volumes between 3 and 500 l;
11. Check to make sure that the diluter does carry out the requested aspiration and dispense (i.e.
watch to ascertain that the required movements are performed). Check for leakage from the
hydraulic connections;
12. Exit the diagnostic programme by clicking on Exit.



Mechanical functional check of the electro-valve diluter

1. Launch the Diagnostic programme from System Monitor;
2. Select the Diagnostic button and wait until the instrument has carried out a reset;
3. Select Diluter;
4. Select the Electro-valve Test button and make sure that the acoustic alarm on the control
board beeps;
5. Click on Exit.

PHOTOMETER MODULE

Replacing the photometer lamp

Figure X Optics

procedure

To replace the lamp proceed as follows:
Remove the photometers protective cover;
Loosen the screws that hold the lamps A terminals and disconnect them;
Unscrew the lamps support base screw B and take out the lamp;
Insert the new lamp without touching the bulb and tighten the B screw to block it;
Reconnect the lamps A terminals and carefully tighten the screws that hold them;
Replace the photometers protective cover;
Carry out an electronic regulation of the photometer as described in section XXX


Electronic regulation of the photometer

Warning: the following required procedure must be carried out for both new lamps and new
reaction sectors.

1. Turn on the Vegasys system and launch the software management programme by double
clicking on the Vegasys icon;
2. Launch the diagnostic programme and wait until the instrument has carried out a reset;
3. Select Change Photometer Lamp;
4. Check to make sure that the voltage present on the lamp wires connecting terminal is 6.0 volt
0.1; if not, rotate the TR1 trimmer located on the Vegasys control board;
5. Make sure that the required bottle is full (distilled water) and that there is no trace of dust or
mould in the bottle that could impair instrument functionality;
6. Select the Auto-zero button;

The Vegasys will automatically sample and aspirate the distilled water 3 times Auto-zeroing the
results expressed in mv and counts are reported in a table that can be viewed on the screen.

INSERIRE UNA IMMAGINE VALIDA; INOLTRE IL CAMPO DENSITA OTTICA E
IL GRAFICO NON DEVENO ESSERE PRESENTI.



The below illustrated tables contain the acceptable range of values as expressed in mv and
counts.

Table A

Filter Minimum Value (mv) Maximum Value (mv)
Dark
340, 405, 492, 510,
546, 578 and 620

Table B

Filter Minimum Value
(Count)
Maximum Value
(Count)
Dark
340, 405, 492, 510,
546, 578 and 620

In the event that the 340 filter and/or other filter(s) are out of range, before regulating either the
Gain or Offset, make sure that:

the reading cell is perfectly clean;
the reading cell is full of liquid and that there are no air bubbles in it.

7. Make sure that the counts value for the Dark filter is equal to XYZ Y0; if not, adjust by
using the Offset trimmer in the Preamp/ADC board;
8. Identify which filter (from 340 nm to 620nm) shows the highest energy level;
9. Make sure that the counts value for this highest energy level filter is between ZZ000 and
WW000. If not, adjust by using the Gain trimmer in the Preamp/ADC board;
10. Select the Filters Reading button;
11. Make sure that the counts values for each filter are between XX000 and YY000. If not, adjust
by using the Gain trimmer in the Preamp/ADC board;



Replacing and regulating the interferential filters

Warning: before performing this calibration procedure make sure that the reading cell is filled with
distilled water.

N. B.: the 340 nm interferential filter does not need to be shielded. All the other filters may
require adequate shielding in order to obtain for each of them an output signal between XX000
YY000 counts.

1. With the instrument turned off remove the outside cover to allow access to the optics module;
2. Remove the protective casing around the optics group;
3. Remove the blocking ring from the 340 nm filter, in the second position on the filters barrel;
4. Mount the new filter (340 nm) in the second position on the filters barrel with the coloured
part facing inwards and then tighten the ring nut.
5. Turn the Vegasys system on, launch the instrument management programme by double clicking
on the Vegasys icon;
6. Launch the diagnostic programme and wait until the instrument has carried out a reset;
8. In the Select Filter field, select the 340 nm filter; the filters barrel will automatically
position the 340 nm filter along the optical axis;
9. Make sure, via the optical path, that the reading cell is filled with distilled water and that there
are no air bubbles in it;
10. Select the Start Reading button;
11. Use the Gain trimmer, positioned on the amplifier board casing, to bring the counts value
reading to ZZ000 counts WW counts;
12. Select the STOP Reading button;
13. Click on Arms/Plate Setting;
14. Select the Motors Off button;
15. Remove the blocking ring from the 405 nm filter, in the third position in the filter barrel;
16. Mount the new filter (405) in the third position of the filter barrel with the coloured part
facing inwards and without forcing the 405 nm filter ring block;
17. Select the General Reset button and wait until the instrument has carried out a reset;
18. Click on Change Photometer Lamp;


19. In the Select Filter field, select the 405 nm filter; the filters barrel will automatically
position the 405 nm filter along the optical axis;
20. Select the Start Reading button;
21. Make sure that the counts value reading is between XX000 YY000 counts;
22. If the counts value reading is not in the XX000 YY000 counts range, proceed as follows:
Select Arms/Plate Setting;
Click on the Motors Off button;
Remove the required filters blocking ring;
Insert one or more equalization shields chosen each time from among the graded
percentages of grey available until the counts value reading for the required filter falls
within the XX000 YY000 interval;
Replace the blocking ring without forcing it;
Repeat for each required filter steps 17 through 22.
23. Select Arms/Plate Setting;
24. Select the Motors Off button;
25. Tighten the blocking ring of the filter in question;
26. For each of the remaining filters (492, 510, 540, 580 and 620) carry out steps 15 through 25 .
Please note: following is a list of the positions occupied by the various filters in the optical filters
barrel
1
st
Position: Dark
2
nd
Position: 340 nm
3
rd
Position: 492 nm
4
th
Position: 510 nm
5
th
Position: 546 nm
6
th
Position: 578 nm
7
th
Position: 620 nm

27. Select the General Rest button and wait until the instrument has carried out a reset;
28. Mount the optics groups protective casing;
30. Click on Start Reading for the Dark filter;
31. Make sure that the counts value reading for the Dark filter falls within the GGG HHH
interval; if not, use the Offset trimmer on the Preamp/ADC board to bring it within said interval.
32. Select the Auto-zero button;


33. Make sure that the counts value reading for each filter falls within the required interval and if
not substitute the relative shield with another having a different graded percentage.

PERISTALTIC PUMP MODULE

Replacing the Peristaltic Pump

Replacing the Peristaltic Pump Motor



Functional Check of the Peristaltic Pump

1. Turn on the Analyser;
2. Launch the Vegasys management software;
4. Enter the required password in the Password Request window;
5. Select Diluter;
6. Select the PRIME button;
7. Make sure that the wash well empties completely, if not make sure that block B is as far as
it can go towards D.

Calibrating the Peristaltic Pump

Warning: place a reagent container filled with distilled water in position R1 of the Analytical Plate.

2. Enter the required password in the Password Request window;
3. Select Peristaltic Pump;
4. Select the Start Calibration button and wait until the liquid dispensed into the reaction
cell is aspirated by the aspiration probe;



5. Make sure that the liquid fills the silicone tubes (going into and coming out of the reading
cell), as illustrated in the drawing below (the portion of the silicone tubes that is coloured
black);

6. In the event that the B portion of the tube shows an excess and the A portion appears
insufficiently filled (those portions of the tube that show the liquid entering and exiting the
reading cell), decrease the number of steps viewed in the Final Aspiration field, select
once more Start Calibration, and recheck the status of the liquid in the tubes. Vice versa,
if there is an excess of liquid in the A portion and the B portion appears insufficiently filled,
increase the number of final aspiration steps and then reselect the Start Calibration
button.
Note: if the above described software adjustment process is not sufficiently effective, regulate
the pressure between the silicone tube and the peristaltic pump rotor.

(A)
(B)
Chapter 08 ERROR SIGNALLING

CHAPTER 08 ERROR SIGNALLING

INDEX

8.1 ERROR SIGNALLING.............................................................................................................. 2
8.1.1 SYSTEM ERRORS .................................................................................................................... 2
8.1.2 RESULTS FLAGS...................................................................................................................... 3


8.1 ERROR SIGNALLING

This charter is dedicated to a description of the error signalling which may occur during the
programming or carrying out of the various operations. Error signals can be divided into the
following twogroups:

System Errors

Result Flags

8.1.1 SYSTEM ERRORS

Whenever a system error is detected,it is signalled via a visual text warning message indicating the
cause of the signalled anomaly.

Following is a list of possible visual text Warning! and Fatal! messages:

Warning messages

Temperature Out of Range
Autozero Out of Range
Host Serial Port Link Missing

Fatal messages

Vertical Sampling Arm Error
Horizontal Sampling Arm Error
Vertical Aspiration Arm Error
Filter Drum Error
ADC Error
Plate Error
Diluter Error
Time Out Error (Generic Error)

8.1.2 RESULTS FLAGS

Every Result Flag, signalling an error, is accompanied by a symbol.
Following is a list of possible flags/indicators:

Flag/Indicator Description
Fit Indicates the linearit of the plot of the measured absorbance
against time for the sample.

Fit = 1.0 is ideal. This means perfect linearity.
Fit < 1.0 can also be acceptable. Check the graph.

R Reagent Blank Level value over the limits setup in the Method
I Inversion.
Reaction direction not in line with that set-up in the Methods.
S Depletion Limit.
Value over the limit set-up in the Methods.

* Concentration value over the Lin. Limit High set-up in the
Methods.
H Concentration value over the max. Normal Value set-up in the
Method.

L Concentration value below the min. Normal Value set-up in the
Method.
E This symbol appears whenever the user has manualy modified
he obtained results. This operation annuls all the symbols
indicating errors which, in this case, will not be viewed.

% Coefficient of Variation Percentage in the Standard Replicates
over the set value.

Chapter 09 - INTERFACING PROCEDURES
CHAPTER 09 - INTERFACING PROCEDURES

INDEX

9.1 CONNECTION CABLE........................................................................................................... 2
9.2 CONNECTION PARAMETERS ............................................................................................. 2
9.3 SOFTWARE PROTOCOL....................................................................................................... 3
9.3.1 FRAME FORMAT................................................................................................................... 3
9.3.2 BEGIN OF FRAME.................................................................................................................. 3
9.3.3 IDENTIFICATION CODE OF FRAME.................................................................................. 3
9.3.4 DATA FIELD........................................................................................................................... 4
9.4 PROCEDURE FOR CONTROL DATA TRANSMISSION.................................................... 6
9.4.1 CONNECTION PHASE (DATA LINK).................................................................................. 6
9.4.2 DATA TRANSFER.................................................................................................................. 6
9.5 TRANSFER OF PATIENTS REQUESTS: HOST > AU.............................................. 7
9.6 TRANSFER OF RESULTS: AU > HOST........................................................................ 8

Vegasys can be connected via a host computer to facilitate patient test requisition and reporting.
This chapter deals on interfacing procedure which can be utilised in making the connection.
9.1 CONNECTION CABLE
Figure 16 shows the pin configuration of serial port RS 232 defining the hardware
connection.

VEGASYS HOST
DB 25 F DB 25 F

DB 9

2 Txd Rxd

3 Rxd Txd

7 SG SG

3

2

7

2

3

5

FIGURE 16

9.2 CONNECTION PARAMETERS
Figure 17 shows the parameters for the communication:

BAUD RATE
DATA BITS
PARITY CHECK
STOP BITS
END CODE

4800
7
E
2
<ETX+CKS>

FIGURE 17

SOH : BEGINNING OF CONNECTION (POLLING) (01H)
STX : BEGINNING OF FRAME
ETX : END OF FRAME
ACK : CONFIRMATION OF RECEPTION OF FRAME
NACK : WRONG RECEPTION OF FRAME
CKS : CHECK SUM

9.3 SOFTWARE PROTOCOL
9.3.1 FRAME FORMAT
Each FRAME transmitted by AU (analyser) and by HOST (computer) is composed of
the following elements:

BEGIN OF FRAME FRAME IDENT. CODE DATA FIELD END OF FRAME

Control characters such as (SOH, ACK and NACK) will be transmitted individually.
9.3.2 BEGIN OF FRAME


BEGIN OF FRAME : STX (O2H) length 1 byte

Each FRAME transmitted by AU and/or by HOST will begin with (STX = O2H).
9.3.3 IDENTIFICATION CODE OF FRAME


BEGIN OF FRAME : See Types of Frame below

The identification code of FRAME represents the type of information that will be
transmitted.
TYPES OF FRAME

N. CHARACTER ASCII CODE TRANSMITTER
1 P 50H HOST
2 R 52H AU
3 D 44H AU

No. 1: Character P identifies the transfer of patients from HOST to AU.
No. 2: Character R identifies the transfer of results from AU to HOST.
No. 3: Character D identifies the transfer of number of patients existing on AU.

9.3.4 DATA FIELD


DATA FIELD : See the following section

The element DATA FIELD is defined depending on the type of identification code of
frame:
a) IDENTIFICATION CODE OF FRAME = P

DATA FIELD (P) = length 88 bytes
b) IDENTIFICATION CODE OF FRAME = R

DATA FIELD (R) = variable length

c) IDENTIFICATION CODE OF FRAME = D

DATA FIELD (D) = length 3 bytes

The composition of DATA FIELD (P) = length 88 bytes:

ITEM LENGTH FORMAT

IDENTIFICATION CODE OF PATIENT

8 ALPHANUMERIC

NAME

30 ALPHANUMERIC

TESTS

50

NUMERIC: rrrr....rrr
r = 0 -1
0 = without request
1 = with request

FIGURE 18
NOTE:
THE VALUE '1' OF TEST FIELD ENABLES THE REQUEST OF THE TEST THAT OCCUPIES THE SAME
POSITION IN THE SCHEDULE OF AU.
The composition of DATA FIELD (R) = variable length

ITEM LENGTH FORMAT

IDENTIFICATION CODE OF PATIENT

8 ALPHANUMERIC

NAME

30

ALPHANUMERIC

N TEST (1)

2

NUMERIC :
range = 1-50
* position of test
Schedule of AU

RESULT (1)

8

ALPHANUMERIC

N TEST (n)

2

NUMERIC

RESULT (n)

8

ALPHANUMERIC

1 = with request

FIGURE 19
NOTE :
THE AU WILL ONLY SEND TESTS WHICH HAVE A RESULT.

The composition of DATA FIELD (D) = length 3 bytes

ITEM LENGTH FORMAT

NUMBER OF EXISTING PATIENTS
INTO ANALYZER

3

NUMERIC
range : 1-100

FIGURE 20

9.4 PROCEDURE FOR CONTROL DATA TRANSMISSION
9.4.1 CONNECTION PHASE (DATA LINK)

TIME OUT = 3 min.

5 sec. 5 sec. 5 sec.
SOH SOH SOH SOH
AU

ACK
HOST

FIGURE 21

In order to proceed to connection, AU will start a phase of polling (sending of character
SOH within intervals of 5 sec) and will remain waiting for the confirmation character
ACK. If the confirmation occurs before the last 3 minutes, AU will send the character
ACK (link made), otherwise there will be a TIME OUT (disconnection).
9.4.2 DATA TRANSFER

TIME OUT = 30 sec.

FRAME FRAME FRAME EOT
AU/ HOST

ACK ACK ACK
HOST/ AU

FIGURE 22

During the transmission phase, TIME OUT is set to 30 sec after which there will be the
INTERRUPTION OF COMMUNICATION. Each work session will end with the character EOT.
If, during the line connection, there would be a NO- ACKNOWLEDGEMENT OF FRAME the
HOST/AU will reply with a NACK and remain waiting for a second transmission of FRAME up to
a maximum of three times. Then, if the FRAME is not received in the right way, the
INTERRUPTION OF COMMUNICATION will intervene.

9.5 TRANSFER OF PATIENTS REQUESTS: HOST > AU

AU :<STX>D000<ETX>CKS

HOST

:<ACK>

HOST

:<STX<PID^PAT1^PATIENT^N.1^^^^^^^^^^^^^^^^^^1100101000
00000000000000000000000000000000000000<ETX>CKS

AU

:<ACK>

HOST

:<STX>PID^PAT2^PATIENT^N.2^^^^^^^^^^^^^^^^^^0000000000
01010000000000000000000000000000000000<ETX>CKS

AU

:<ACK>

HOST

:<STX>PID^PAT3^PATIENT^N.3^^^^^^^^^^^^^^^^^^100010000
000100000000000000000000000000000000000<ETX>CKS
AU :<ACK>
HOST :<EOT>

Text format for this case (Transfer of patients requests):

<STX> D000 <ETX> CKS

( transmitted by AU )

<STX>
D
000
<ETX>
CKS
FRAME start
FRAME identification agent
Number of existing patients on AU
End of FRAME
Check sum
<ACK> (transmitted by HOST)
<ACK> Confirm. of right reception of frame
<STX>PID^PATIENT^N.1^^^^^^^^^^^^^^^^^
^1100101000
0000000000000000000000000000000000000<
EXT>CKS

(transmitted by Host)
<STX>
P
ID^PAT1^
PATIENTS^N.1^^^^^^^^^^^^^^^^^^
11001010000000000000000000
<ETX>
CKS
FRAME start
FRAME identification agent
PATIENTS identification agent
Name of patient
Requested tests: 1 - 2 - 5 - 7 of AU schedule
End of frame
Check sum
<ACK> ( transmitted by AU )
<ACK> Confirm. of right reception of FRAME
NOTE: Character ^ = (20H)

9.6 TRANSFER OF RESULTS: AU > HOST

AU :<STX>RID^PAT1^PATIENT^N.1^^^^^^^^^^^^^^^^^^0127^^^^^^
0231^^^^^^051.2^^^^^07160^^^^^<ETX>CKS
HOST :<ACK>
AU :<STX>RID^PAT2^PATIENT^N.2^^^^^^^^^^^^^^^^^^1238^^^^^^
14160^^^^^<ETX>CKS
HOST :<ACK>
AU <STX>RID^PAT3^PATIENT^N.3^^^^^^^^^^^^^^^^^^0127^^^^^^
051.2^^^^^1238^^^^^^<ETX>CKS
HOST :<ACK>
AU :<EOT>

Text format for this case (Transfer of results )
<STX>RID^PAT1^N.1^^^^^^^^^^^^^^^^^^0127
^^^^^^0231^^^^^^051.2^^^^^07160^^^^^<ETX>C
KS
( transmitted by AU )
<STX>
R
ID^PAT1^
PATIENTS^N.1^^^^^^^^^^^^^^^^^^
01
27^^^^^^
02
31^^^^^^
03
1.2^^^^^^
07
160^^^^^^
<ETX>
<CKS>
FRAME start
FRAME identification code
PATIENT identification code
Name of patient
Test no. 1
Result no. 27
Test no.2
Result no. 31
Test no. 3
Result no. 1.2
Test no. 7
Result no. 160
End of FRAME
Check sum
<ACK> (transmitted by HOST)
<ACK> Confirm. of right reception of FRAME

CHECK SUM DETERMINATION:
ASCII sum of all characters of the FRAME ( excluding STX and ETX ).
Module 256 = 0.
Example:
FOR I =1 TO LEN (FRAMES)
CKS! = CKS! + ASC ( MIDS FRAMES, I, 1 )
NEXT I
Check sum = RIGHT ("0"+ HEX (256 - (CKS! (MOD 256) ) ), 2 )
Chapter10 - TROUBLESHOOTING


CHAPTER 10

- TROUBLESHOOTING GUIDE -

P R O B L E M

POSSIBLE CAUSE/RIMEDY
THE SAMPLING PROBE DOES NOT
ASPIRATE.
The probe could be obstructed; plunge the
probe.
If the diluter module works, check if there
are squeezed or torn tubing.
THE SAMPLING PROBE DOES NOT
DISPENSE THE EASH SOLUTION
INTO THE WASHING WELL
Chech the valve of the diluter.
Check if there is enough wash solution in the
bottle.
Check all tubing connection.

REPEATIBILITY OD RESULTS
INSUFFICIENT

Sampling probe dirthy.
Hydraulic leak and/or air bubbles in the
hydraulic circuit (sampling).
Check sampling probe fit.
Check the fit of the hydraulic tubes and their
connection: if necessary replace the tubes
and/or adjust their connection fittings.
Deterioration of the Reagent (s).
Substitute the bad reagent(s).
Light bulb not stable..
Change the light bulb following the
procedure described in Chapter 06
Maintanance.
Check the probe cleaner pad. It may be
dirthy. In this case, change the probe cleaner
pad.
Check the flow cell. Make sure it is clean; if
necessary, carry out the procedure described
in Chapter 06 Maintanance.
THE ASPIRATION INTO THE FLOW
FLOW CELL IS NOT CONSISTENT
The aspiration probe may be dirthy or
obstructed.
The tubing may be torn or obstructed.
Check that the probe descend enough below
the liquid level to correctly aspirate.
THE ASPIRATION ARM DESCENDS
ENOUGH BELOW THE LIQUID
LEVEL TO CORRECTLY ASPIRATE
BUT DOES NOT ASPIRATE
The silicon tubing of the peristaltic pump
may be torn.
The peristaltic pump rotori s too tight.
The probe may be obstructed or unhooked.


THE WASHING WELL IS NT
EMPTIED EVEN IF THE
PERISTALTIC PUMP WORKS
PROPERLY

The tubing from the washing well to the
electro-valve may be torn or not properly
connected.
Check the electro-valve (of the aspiration
circuit).
Check the peristaltic pump tubing.

I VALORI DI AUTOZERO SONO
TUTTI INFERIORI A XXXXX COUNT

The microflow cell may be empty. Fill it with
water.
The peristltic pump may not be working
properly. Check if tubings need to be
changed.
Check the aspiration probe and its
connection with the microflow cell. The may
be some leakage or air bubbles in the
micoflow cell, or the probe may be dirty.
The wash solution bottle may be empty.
Check the peristaltic pump electro-valve.
Adjust the aspiration volume; there may be
too much or to little that has been aspirated.
The microflow cell is dirty or air bubbles get
into the cell.

I VALORI DI AUTOZERO SONO
TUTTI INFERIORI AI XXX COUNT

Light bulb nearing the end of its life cycle or
premature deterioration. In both cases,
change the light bulb following the
procedure described in Chapter 07-
Maintenance.

THE RESULTS OF THE CONTRLS
ARE OUT OF RANGE

Make sure that the controls have been
analyzed with he methods specified by the
manufacturer.
Make sure that the settings are correct for the
following parameters: wavelenght,
temperature, sampling volume, reagent
volume, factor.
Make sure that the water used to prepare the
reagent and controls is either bi-distilled or
deionized water.
Check reagents, controls and standards Make
sure that they have been prepared in
accordance with the instructions given by the
manufacturer.
Check that the recommended wash solution
was used and not simply distilled water.


KINETICS TESTS (PARTICULARLY
GOT OR GPT) GIVE LOW RESULTS.
The microflow cell may be contaminated.
Clean the microflow cel following the
procedure described in Chapter 07
Maintenance.
The reagent container may be contaminated.
The reagent may be expired or not fresh.
Check if the correct factor is used..
Check the volume settings.
Check the filter.
Check the temperature.

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