DPPH Radical Scavenging Activity

Table 1. Absorbance at 517nm and DPPH radical scavenging activity of ascorbic acid solutions
Concentratio
n of Ascorbic
Acid (g/mL)

Absorbance at 517nm

Percent Radical Scavenging Activity (%)

Trial 1

Trial 2

Trial 3

Trial 1

Trial 2

Trial 3

Mean

0.87312

0.87311

0.86221

0.78133

0.78131

0.77516

10.515

10.514

10.096

10.375

0.74948

0.74933

0.76183

14.161

14.177

11.642

13.327

0.66849

0.66856

0.58282

23.437

23.428

32.404

26.423

0.58345

0.58473

0.55876

33.176

35.129

35.194

33.800

10

0.32013

0.32263

0.32179

63.335

63.048

62.678

63.020

Table 2. Absorbance at 517nm and DPPH Radical Scavenging Activity of fractions of ethanolic
extract of ESP 15 gathered during Dry season at 100g/mL.

ESP 15 DRY
Absorbance at 517nm
Trial 1
Trial 2
Trial 3

Trial 1

% Radical Scavenging Activity

Trial 2
Trial 3
Mean

SD

Blank
Crude
Fraction

0.86948
0.82089

0.86948
0.81258

0.86948
0.82423

0
5.588

0
6.544

0
5.5204

0
5.779

0.000
0.690

0.84453

0.85422

0.85016

2.870

1.755

2.222

2.282

0.560

2
3
4
5
6

0.85851
0.77155
0.84579
0.83931
0.85973

0.85394
0.77167
0.84457
0.84138
0.84209

0.85376
0.77652
0.84482
0.85054
0.84218

1.262
11.263
2.725
3.470
1.121

1.787
11.249
2.865
3.232
3.150

1.808
10.691
2.836
2.178
3.140

1.619
11.068
2.809
2.960
2.470

0.309
0.326
0.074
0.688
1.169

Table 3. Absorbance at 517nm and DPPH Radical Scavenging Activity of fractions of ethanolic
extract of ESP 15 gathered during Wet season at 100g/mL.

ESP 15 WET
Absorbance at 517nm

% Radical Scavenging Activity

Trial 1

Trial 2

Trial 3

Trial 1

Trial 2

Trial 3

Mean

SD

Blank

0.86948

0.86948

0.86948

Crude

0.82857

0.82386

0.82431

4.705

5.247

5.195

3.352

0.299

0.79263

0.79562

0.79524

8.839

8.495

8.510

8.615

0.194

0.83675

0.84063

0.84338

3.318

3.318

3.002

3.361

0.383

0.84489

0.84569

0.84601

2.828

2.736

2.699

2.754

0.066

0.84851

0.84855

0.84205

2.412

2.407

3.155

2.658

0.430

0.80608

0.81027

0.80623

7.292

6.810

7.274

7.125

0.273

0.82738

0.82700

0.82632

4.842

4.886

4.964

4.897

0.062

Fraction

FERRIC REDUCING ANTIOXIDANT POWER

Table 4. Absorbance at 595nm of FeSO47H2O solutions.

FeSO47H2O
(uM)

Absorbance at 595nm
Trial 1
0.21478
0.24626
0.28418
0.36035
0.46013
0.52997

0
62.5
250
500
750
1000

Trial 2
0.24626
0.24561
0.28436
0.36040
0.46021
0.52977

Trial 3
0.24634
0.24634
0.28319
0.36188
0.46053
0.53030

Average
0.21478
0.24607
0.28391
0.36088
0.46029
0.53001

Table 5. Absorbance at 595nm and Ferric Reducing Ability Power of ESP 15- DRY
ethanolic extract at 100g/mL.

ESP 15 - DRY
Absorbance at 525nm

Reducing Power (mmol Fe2+/g fraction)

Trial 1

Trial 2

Trial 3

Trial 1

Trial 2

Trial 3

Mean

SD

0.70853

0.71726

0.70954

2.484

2.526

2.489

2.500

0.023

0.32936

0.33153

0.32944

0.679

0.689

0.679

0.682

0.006

0.73589

0.73613

0.74106

2.615

2.616

2.639

2.623

0.014

0.63144

0.63167

0.61234

2.117

2.118

2.026

2.087

0.053

0.29108

0.28621

0.29110

0.497

0.473

0.497

0.489

0.014

0.32056

0.32062

0.30479

0.637

0.637

0.562

0.612

0.043

0.36028

0.36407

0.36427

0.826

0.844

0.845

0.838

0.11

Crude
Fraction

Table 6. Absorbance at 595nm and Ferric Reducing Ability Power of ESP 15- WET
ethanolic extract at 100g/mL.

ESP 15- WET

Absorbance at 595nm

Reducing Power (mmol Fe2+/g fraction)

Trial 1

Trial 2

Trial 3

Trial 1

Trial 2

Trial 3

Mean

SD

0.70923

0.70128

0.69877

2.488

2.450

2.438

2.459

0.026

0.44502

0.44499

0.44496

1.230

1.229

1.229

1.229

0.072

0.43036

0.43030

0.43193

1.160

1.160

1.167

1.162

0.004

0.44992

0.44669

0.44673

1.253

1.238

1.238

1.243

0.009

0.28804

0.28807

0.29497

0.482

0.482

0.515

0.493

0.019

0.36710

0.36726

0.37965

0.859

0.918

0.918

0.879

0.034

0.29248

0.28783

0.28963

0.503

0.481

0.490

0.491

0.011

Crude
Fraction

Ferrous Ion Chelating Activity

Table 7. Absorbance at 562nm and Ferrous ion-Chelating ability of EDTA solution
Concentration
of EDTA
(g/mL)
0
5
10
12.5
20
25

Absorbance at 562nm

Percent Chelation (%)

Trial 1

Trial 2

Trial 3

Trial 1

Trial 2

Trial 3

Mean

1.09236

1.09236

1.09236

0.58345

0.58473

0.55876

33.176

33.029

35.194

33.8

0.58245

0.58249

0.58247

33.291

33.286

32.445

33.007

0.52578

0.52574

0.52577

39.781

39.785

39.021

39.529

0.25058

0.25058

0.25058

71.301

71.300

70.937

71.179

0.05232

0.05238

0.05245

94.008

94.001

93.917

93.975

Table 8. Absorbance at 562nm and Ferrous ion-Chelating ability of fractions of ethanolic extract
of ESP 15 gathered during Dry season at 100g/mL.

ESP 15 DRY
Absorbance at 562nm

% Radical Scavenging Activity

Trial 1

Trial 2

Trial 3

Trial 1

Trial 2

Trial 3

Mean

SD

Blank

1.09583

1.09583

1.09583

0.000

Crude

0.62981

0.62991

0.62991

42.527

42.518

42.518

42.521

0.005

0.34515

0.34514

0.34514

68.503

68.504

68.504

68.504

0.001

0.47231

0.47218

0.47218

56.899

56.911

56.911

56.907

0.007

0.63889

0.63889

0.63884

41.698

41.698

41.703

41.7

0.003

0.50655

0.50655

0.50655

53.775

53.775

53.775

53.775

0.000

0.48328

0.48312

0.48288

55.898

55.913

55.935

55.915

0.019

0.05959

0.05962

0.05957

94.562

94.559

94.564

94.562

0.003

Fraction

Table 9. Absorbance at 562nm and Ferrous ion-Chelating ability of fractions of ethanolic extract
of ESP 15 gathered during Wet season at 100g/mL.

ESP 15 WET
Absorbance at 562nm
Blank

% Radical Scavenging Activity

Trial 1

Trial 2

Trial 3

Trial 1

Trial 2

Trial 3

Mean

SD

1.09583

1.09583

1.09583

0.62991

20.552

20.573

20.579

20.568

0.014

Crude
Fraction
1

0.41634

0.41634

0.41635

62.007

62.007

62.006

62.007

0.001

0.40199

0.40198

0.40196

63.316

63.317

63.319

63.317

0.002

0.46106

0.46100

0.46107

57.926

57.931

57.925

57.927

0.003

0.28813

0.28804

0.28810

73.707

73.715

73.709

73.710

0.004

0.06502

0.06505

0.06502

94.067

94.064

94.067

94.066

0.002

0.17888

0.17886

0.16879

83.676

83.678

84.597

83.984

0.531