Genetic variation of CAT mRNA gene

in eukaryotic mammalia
Philip W. Chrich, University of Southampton
Catalase is a ‘Catalase’ is was placed into (NM_214301.1),
vital enzyme coded for by the BLAST (Basic Homo sapiens
which converts gene ‘CAT’. As Local Alignment (NM_001752.3),
hydrogen seen in the Search Tool) [2] Pongo abelii
peroxide (a Bioinformatics and a nucleotide (NM_001131267.
waste product experiment which BLAST search 1) and Canis
of many I have carried was performed to lupus familiaris
metabolic out, a very find any (NM_001002984.
processes) into similar form of organisms which 1). The reason I
water and the gene coding possessed the chose these 5
oxygen. I for the enzyme is same/similar organisms was
performed an present in many sequence within because these
investigation different their genomes. were the most
into the organisms, and closely related
variation of the the difference is organisms in
gene across a minimal. The This was the terms of the
number of enzyme Catalase single strand of coded sequence,
species of is essential in the DNA used: excluding any
animals by catalysis of the with partial CDS,
performing a decomposition of tgtcctgaccactgga synthetic,
BLAST search hydrogen ggtggtaatccagta predicted and
using a peroxide into ggagacaaactcaat unnamed
sequence of water and agtctgacagtaggg sequences.
single stranded oxygen by the ccccgagggccccttc Within the detail
DNA and an equation: tcgtccaggatgtggt of the search hits
EMBOSS tttcactgatgaaatg were the
search, to 2 H2O2 → 2 H2O + gctcactttgaccggg sequences of
compare the O2 [1] agagaattcctgaga amino acids
translational As hydrogen gagtcgtgcacgcca which were
protein peroxide is aaggagcaggggctt coded for by the
sequence. From formed as a by- ttggctactttgaggtc strand of DNA.
this I was able product of many acacatgacattacca This information
to construct a metabolic gatactccaaggcga was placed into a
phylogenetic processes, nearly aggtgtttgagcatatt comparison
tree showing all known ggaaa search using
graphically the eukaryotae EMBOSS [3], part
difference in Then, 5 of the European
possess a form of organisms which
the gene the enzyme, and Molecular Biology
between possessed the Laboratory. The
most prokaryotae same gene
species, and also possess it. sequences of
was able to (mRNA CAT) were amino acids in
come to a MA chosen from an each organism
conclusion TERIALS AND extensive list. were compared
about the METHODS These organisms against each
similarity. which were other, in every
Primarily the chosen were Bos combination
The gene that sequence of the taurus possible, to
codes for the given single (NM_001035386), identify
enzyme stranded DNA Sus scrofa similarities in the
 coded amino acid XM_508362.2 PREDICTED The EMBOSS CDS
Pan
sequence, and troglodytes comparison
hence the catalase results received
presence of the gave scores of
All of the results I
same enzyme. between 186 and
chose had a
The similarity 267.
maximum
score was then Phylogenetic tree
identity of over
noted, and will be constructed from
90%, which
used to formulate above results.
makes my results
a phylogenetic From the
highly reliable,
tree. This tree EMBOSS
and the total
will show the comparison
score for all of
similarity of the results I
my chosen
gene coding for concluded that
organisms were
the Catalase the Pongo abelii
enzyme, and and Homo
Organism Score
detailed sapiens CAT
comparison
formulation of genes were very
the tree will be Bos taurus + Sus scrofa 211.0 similar, having
discussed in the Bos taurus + Homo 199.0
the highest score
next section. sapiens (267.0), as both
species are
Bos taurus + Pongo 200.0
primates, and
abelii
Homo sapiens
Bos taurus + Canis 213.0 are evolved from
lupus familiaris
primates, and
RESULTS Sus scrofa + Homo 186.0 hence have very
sapiens similar genes.
Here is a list of Sus scrofa + Pongo 189.0 These two
some of the abelii organisms would
results from the be placed on the
Sus scrofa + Canis 219.0
BLAST search: lupus familiaris same branch.
Next, I concluded
Accession No. Description Pongo abelii + Homo 267.0
that Bos taurus
sapiens
would be placed
Pongo abelii + Canis 209.0 on a branch on
lupus familiaris
NM_00103538 Bos taurus its own on a
6.1 (CAT) mRNA phylogenetic
Homo sapiens + Canis 213.0
GQ204786.1 Capra hircus lupus familiaris tree, as Sus
(CAT) mRNA scrofa and Canis
NM_214301.1 Sus scrofa
over 340. lupus familiaris
(CAT) mRNA have the next
D89812.1 Sus scrofa higher
(CAT) mRNA comparison
NM_001752.3 Homo scores than Bos
sapiens taurus do with
(CAT) mRNA
any of the 5
AK291585.1 Homo species, but I
sapiens
placed them on a
cDNA
sub-branch of has obviously [3]http://www.ebi oss/align/index.ht
Bos taurus, as been a slight .ac.uk/Tools/emb ml
the three gave mutation in the
quite close gene which codes
scores. for the catalase
enzyme between
Sus scrofa and
DISCUSSION Bos taurus
From the somewhere along
EMBOSS the evolutionary
comparison pathway. The
scores and the conclusion I have
phylogenetic tree come to is that
which I have even though
constructed, it is there are slight
plain to see that changes in the
the primates gene which codes
(Homo sapiens for the Catalase
and Pongo abelii) enzyme, it is
have very similar similar enough to
CAT genes, which determine that
could explain millions of years
Homo sapiens ago, there would
evolution from have only been
primates, one such gene,
although many and as evolution
more tests would has taken its
have to be course, this
conducted for single gene has
further evidence. mutated and
The taxonomic been expressed
orders of Sus in many different
scrofa, Canis organisms. This
lupus familiaris supports the
and Bos taurus theory that all
Artiodactyla, known species
Carnivora and descended from
Artiodactyla, a single common
respectively, ancestor, and
which would gene pool.
suggest that Bos
taurus and Sus REFERENCES
scrofa would be [1]http://www.cat
on the same alase.com/cataex
branch on the t.htm
tree, but the [2]http://blast.nc
EMBOSS results bi.nlm.nih.gov/Bl
would suggest ast.cgi
otherwise. There