Zinc is an essential element and cause deleterious effect at high concentration to both the animals and plants. In the present study, we observed that the Zinc chloride at lethal concentration fifty alters the behavior of fish which also change the physico-chemical properties of water. It was observed that the Zinc chloride steadily increased the pH, conductivity, free carbon dioxide and total alkalinity of water from 24 to 96 hrs, whereas the dissolved oxygen concentration in water was gradually decreased. It is concluded that the increase in pH, conductivity, free carbon dioxide and total alkalinity of water and decrease in oxygen could be due to increase in the metabolic processes of Ophiocephalus punctatus upon exposure to zinc chloride at lethal concentration 44.25 mg/l.
Article Citation:
Swapan S. Bacher and Arun M. Chilke.
Effect of Zinc Chloride (LC-50) on physicochemical parameters of water upon exposure to Ophiocephalus punctatus.
Journal of Research in Animal Sciences (2012) 1(1): 007-012.
Full Text:
http://janimalsciences.com/documents/AS0007.pdf
Original Title
Effect of Zinc Chloride (LC-50) on Physicochemical Parameters of Water Upon Exposure to Ophiocephalus Punctatus
Zinc is an essential element and cause deleterious effect at high concentration to both the animals and plants. In the present study, we observed that the Zinc chloride at lethal concentration fifty alters the behavior of fish which also change the physico-chemical properties of water. It was observed that the Zinc chloride steadily increased the pH, conductivity, free carbon dioxide and total alkalinity of water from 24 to 96 hrs, whereas the dissolved oxygen concentration in water was gradually decreased. It is concluded that the increase in pH, conductivity, free carbon dioxide and total alkalinity of water and decrease in oxygen could be due to increase in the metabolic processes of Ophiocephalus punctatus upon exposure to zinc chloride at lethal concentration 44.25 mg/l.
Article Citation:
Swapan S. Bacher and Arun M. Chilke.
Effect of Zinc Chloride (LC-50) on physicochemical parameters of water upon exposure to Ophiocephalus punctatus.
Journal of Research in Animal Sciences (2012) 1(1): 007-012.
Full Text:
http://janimalsciences.com/documents/AS0007.pdf
Zinc is an essential element and cause deleterious effect at high concentration to both the animals and plants. In the present study, we observed that the Zinc chloride at lethal concentration fifty alters the behavior of fish which also change the physico-chemical properties of water. It was observed that the Zinc chloride steadily increased the pH, conductivity, free carbon dioxide and total alkalinity of water from 24 to 96 hrs, whereas the dissolved oxygen concentration in water was gradually decreased. It is concluded that the increase in pH, conductivity, free carbon dioxide and total alkalinity of water and decrease in oxygen could be due to increase in the metabolic processes of Ophiocephalus punctatus upon exposure to zinc chloride at lethal concentration 44.25 mg/l.
Article Citation:
Swapan S. Bacher and Arun M. Chilke.
Effect of Zinc Chloride (LC-50) on physicochemical parameters of water upon exposure to Ophiocephalus punctatus.
Journal of Research in Animal Sciences (2012) 1(1): 007-012.
Full Text:
http://janimalsciences.com/documents/AS0007.pdf
Effect of Zinc Chloride (LC-50) on physicochemical parameters of water
upon exposure to Ophiocephalus punctatus
Keywords: Zinc, Physico-chemical parameter, Ophiocephalus punctatus. 007-012 | JRAS | 2012 | Vol 1 | No 1 Ficus Publishers. This Open Access article is governed by the Creative Commons Attribution License (http:// creativecommons.org/licenses/by/2.0), which gives permission for unrestricted use, non- commercial, distribution, and reproduction in all medium, provided the original work is properly cited. Submit Your Manuscript www.ficuspublishers.com http://ficuspublishers.com/ Authors: Swapan S. Bacher and Arun M. Chilke.
Institution: Division of Toxicology and Biomonitoring, Department of Zoology, Shree Shivaji Arts, Commerce and Science College, Rajura-442805 (India).
Corresponding author: Arun M. Chilke.
Email: achilke.2011@rediffmail.com
Web Address: http://ficuspublishers.com/ documents/AS0007.pdf Dates: Received: 05 Mar 2012 /Accepted: 15 Mar 2012 /Published: 04 Apr 2012 Article Citation: Swapan S. Bacher and Arun M. Chilke. Effect of Zinc Chloride (LC-50) on physicochemical parameters of water upon exposure to Ophiocephalus punctatus. Journal of Research in Animal Sciences (2012) 1: 007-012 An International Online Open Access Publication group Original Research J o u r n a l
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Journal of Research in Animal Sciences ABSTRACT:
Zinc is an essential element and cause deleterious effect at high concentration to both the animals and plants. In the present study, we observed that the Zinc chloride at lethal concentration fifty alters the behavior of fish which also change the physico-chemical properties of water. It was observed that the Zinc chloride steadily increased the pH, conductivity, free carbon dioxide and total alkalinity of water from 24 to 96 hrs, whereas the dissolved oxygen concentration in water was gradually decreased. It is concluded that the increase in pH, conductivity, free carbon dioxide and total alkalinity of water and decrease in oxygen could be due to increase in the metabolic processes of Ophiocephalus punctatus upon exposure to zinc chloride at lethal concentration 44.25 mg/l. Journal of Research in Animal Sciences An International Open Access Online Research Journal
INTROCUTION: Heavy metals accumulate in the aquatic organism through various sources like sediments, soil erosion and runoff, air depositions of dust, aerosol, and discharge of waste water (Labonne et al., 2001; Goodwin et al., 2003). But majority of aquatic ecosystems are greatly affected by the industrial waste containing variety of heavy metals. Heavy metals from industries not only disturb the aquatic environment but leads to environmental health hazards (Shukla et al., 2007; Gupta and Srivastava, 2006; Agtas et al., 2007; Yoon et al., 2008). Zinc is a common trace metal essential in minute quantities for mammal and fish (Frieden, 1972 and Holcombe et. al., 1979), and is generally regarded as one of the less hazardous element, though its toxicity may be enhanced by the presence of other heavy metals like cadmium, copper and ferrous etc. However small quantities of zinc are required for normal development and metabolism of organisms, if levels exceed the physiological requirements, zinc can act as a toxicant. Exposure to excess zinc has been reported to bring about biochemical as well as histological changes in various organs of fishes (Agrawal and Srivastava, 2003; Srivastava and Verma, 2009). Indeed, much work is available on the effect of zinc on various aspects of animal behavior, histopathology, biochemical and histochemical variation etc. But very little work has been done with respect to effect of heavy metal on physicochemical change of water (Bacher and Chilke, 2012) upon exposure to fish in general. In the present work the attempt was made to fill out the lacunae by correlating the effect of zinc on alteration of physicochemical properties of water upon exposure to Ophiocephalus puctatus.
MATERIALS AND METHODS: Healthy fingerlings of Ophiocephalus puctatus measuring the size 12-15cm were purchased from fisherman of Mulchera (District-Gadchiroli). It was carried to the laboratory in hygienic condition and acclimatized for fifteen days. During this period of acclimatization fish were fed with boiled eggs and rice bran. Zinc LC-50 for 96 hrs was carried out thrice to confirm exact dose of LC-50. Then the fish were divided into six groups. Each group was contained twenty fish each. Later all these fish were exposed to lethal concentration of Zinc for 24, 48, 72 and 96 hrs. The estimation of water was carried out at the interval of 24 hrs after real start time. All the physicochemical parameters were tested according to APHA (1989). Values were calculated by one way ANOVA with software Graph pad-5 demo version.
RESULTS: It fact the zinc is an essential inorganic element requires to perform normal physiological function. Deficiency of this element affects the general physiology. However the higher concentration causes the detrimental effect to all the aquatic organisms. In the present study we observed that the Ophiocephalus punctatus exhibited 50% mortality at 44.25mg/l of zinc chloride. At 50% lethal concentration of zinc chloride fish exhibited notable changes in its behavior like continuous fin movement, intermittent twitch and rapid opening and closing of operculum and mouth. All these activities were much more frequent in initial time of exposure compared to the later period but remained higher than the control throughout the experimental period. It was observed that the exposed fish secreted large quantity of mucus from 24 hrs to 96 hrs and till 96 hrs caudal region became blood red colored (Fig. 1). Atmospheric temperature was constant (25C) during experimental tenure i.e. from 24 to 72 hours of exposure. However, the water temperature was gradually increased from 24 to 72 hrs. and at 96 hrs it was observed to decrease (Table-1, Fig. 2). Bacher and Chilke, 2012 008 Journal of Research in Animal Sciences (2012) 1: 007-012 The water conductivity was steadily increased (Table-1, Fig. 3) from 24 to 96 hrs, the maximum conductivity (0.519 0.006) was observed at 96 hrs. and lowest at 24 hrs (0.379 0.011). However the pH (negative logarithmic hydrogen ion concentration) also increased perpetually from 24 to 96 hours of exposure (Fig. 4). The maximum pH (6.84 0.013) was noticed on fourth day (96 hrs) of exposure and minimum (6.35 0.009) at 24 hrs. Dissolved oxygen (DO) in water was maximum (Fig. 5) at 24 hrs (0.68 0.87 mg/l) and minimum (0.28 0.60 mg/l) was recorded at 98 hrs. The DO was observed decreasing consistently from 24 to 96 hrs of exposure. But opposite results were obtained for the free carbon dioxide. The free carbon dioxide was continuously increased from 48 to 96 hrs. (Fig. 6), it was recorded minimum at 24 hrs and maximum at 96 hrs of exposure. It was also observed that the total alkalinity as calcium carbonate increased consistently similar to free carbon dioxide from 24 to 96 hrs. The total alkalinity was noted minimum at 24 hrs and maximum at 96 hrs (Fig. 7).
DISCUSSION: Zinc has been recognized to play a vital role in almost all aspects of living systems either directly or indirectly (Alabaster and Lloyd, 1982 and Shukla et al., 2003). When zinc occurs at higher levels than normal, it can act as a pollutant (Agrawal and Srivastava, 2003). However, no report is available on the extent of changes in the physicochemical factors of water after exposure of fish to the LC-50 of Zinc chloride. Therefore the present study was undertaken to find out the induction of change in the physicochemical factors of water upon exposure of Bacher and Chilke, 2012 Journal of Research in Animal Sciences (2012) 1: 007-012 009 Fig 1. Ophiocephalaus punctatus showing an excess secretion of mucous and redness of caudal fin on exposure to Zinc Chloride LC-50 up to 96 hrs. Fig 2. Showing the difference in Ambient and Water Temperature during the experiment from 24hrs to 96hrs upon exposure of Ophiocephalaus punctatus to Zinc LC-50. Fig 3. Showing change in Water Conductivity from 24hrs to 96hrs upon exposure of Ophiocephalaus punctatus to Zinc LC-50.
Ophiocephalus punctatus to the Zinc chloride. The murrel O. punctatus is well known to live in oxygen deficit polluted water in the Indian fresh water ecosystem due to presence of accessory respiratory organs. Bacher and Chilke (2012) have reported the effect of Cadmium LC-50 on the alternation of physicochemical characteristics of water. It has been observed that the ambient temperature influence the temperature of aquatic media and in general temperature of water is always lower than the ambient temperature. The Zinc at LC-50 were observed to decrease initially value of temperature till 72 hrs of exposure and later in 96 hrs that got insignificantly increased. This decrease in temperature could be due to decrease in basic metabolic rate of fish. It was observed that the fish showed continuous movement of mouth, operculum and fins but fish was quiescent. However in the last period of experiment from 72 to 96 hrs the entire activity was seemed to increase as compared to earlier period along with excessive secretion of mucus. This could be implicated to slightly increase in basic metabolic rate of fish till 96 hrs of incubation. The overall conductivity of water consistently increased from 24 to 96 hrs of exposure and this increase in water conductivity could be due to successive increase in the ionic strength of water. It could be concluded that the zinc at above tolerance capacity induce the loss of ions by the fish. The pH of water gradually changed from the first day of exposure till 96 hrs was reported (Bacher and Chilke, 2012) upon exposure of fish to cadmium chloride LC-50. The same was observed in the present study that zinc also induces the loss of protons by the Bacher and Chilke, 2012 010 Journal of Research in Animal Sciences (2012) 1: 007-012 Fig 5. Showing alternation in Dissolved Oxygen in water during the experiment from 24hrs to 96hrs upon exposure of Ophiocephalaus punctatus to Zinc LC-50. Table-1. Effect of Zinc chloride (LC-50) on Physicochemical Parameters of Water Sr.No. Parameters 24-Hrs 48-Hrs 72-Hrs 96Hrs. 1 Ambient Temp. 25 C 25C 25C 25C 2 Water Temp. 24C 24C 23.2C 23.3C 3 Conductivity 0.379 0.011 0.452 0.006 0.483 0.008 0.519 0.006 4 pH 6.35 0.009 6.43 0.011 6.70 0.013 6.84 0.013 5 Dissolved O 2 0.68 0.87 0.58 0.60 0.45 0.60 0.28 0.60 6 Free CO 2 15.48 0.14 21.51 0.28 23.39 0.11 27.69 0.15 7 Total Alkalinity 23.67 0.42 25.5 0.43 27.33 0.49 32.67 1.05 Fig 4. Showing change in pH (logarithmic hydrogen ion concentration) during the experiment from 24hrs to 96hrs upon exposure of Ophiocephalaus punctatus to Zinc LC-50. fish at lethal concentration-50. The gases like the oxygen and the carbon dioxide exhibited dramatic change from 24 to 96 hrs. It was observed that quantitatively the level of oxygen decreased and contrary to this the level of carbon dioxide increased. This finding indicates the rate of oxygen consumption increased and hence the carbon dioxide level in water increased might be due to increase in metabolic rate of fish. The total alkalinity as calcium carbonate was also increased from 24 to 96 hrs of exposure could be due to loss of calcium by the fish which reacts with the excess of carbonic acid to form the calcium carbonate.
ACKNOWLEDGEMENT Authors are very thankful to the Principal, Shree Shivaji Arts, Commerce and Science College, Rajura (M.S.) for providing the laboratory facilities.
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