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Introduction
To study gastrointestinal diseases in Pig farms in
Venezuela, an invasive method by using videoendoscopy
with a fexible fberendoscope was standardized. Previous
study early published (1) have shown that modern imaging
technology is a useful tool to study Porcine Ileitis with a
noninvasive techniques such as ultrasound of the abdomen
of pigs with Proliferative porcine enteritis (PPE). The present
protocol has proven to be a feasible, accurate and highly specifc
procedure to evaluate the mucosa of the gastrointestinal tract
after aspirating gastro-duodenal fuids lavage (GDFL), biopsy
tissues sampling for molecular biology, ultrastructure and
microbiology virus-bacterial isolation, among other uses.
Materials and Methods
A frst step study was run evaluating a total of 150
stomachs, 50 obtained from the slaughter house from fatter
pigs, 50 newborn piglets studied and 50 pigs 12 week old
studied at PRRS (-) PCV2 (+) farms. An exploration of
the Stomach was done from each one. Those areas were
videoendoscoped and handled with a fexible fberendoscope
(Eickemeyer, Fig.1a,b,c,d), perfectly identifed. Thereafter,
the 50 stomachs from the slaughter were opened and the tracts
of the endoscope verifed (Fig.2a,b,c). Gastroduodenal fuid
(GDF) was withdrawn by vaccum pump (EasyVac). Thirty mL
of GDF were collected in sterile containers for bacterial virus
isolation. Paired Biopsies were taken from cardiac fundic -
pyloric and duodenal regions normal and ulcerated lesions
(Fig.3a,b,Fig.2c). One for histopathology (Fig.3b,c) and the
other homogenized for Helycobacter spp. and PCV2 DNA
extraction. The prevalence of Porcine Gastro Duodenal Ulcers
Syndrome (PGDUS) was assessed. A second step study of the
endoscopy protocol was done on 50 newborn-weaned pigs
and 50 pigs 12 week old after 12 hours fasting (Fig.2b). The
latter pigs were previously anesthetized with Xylazine (22 mgr/
Kg) / Ketamine (2 mgr/Kg). After relaxation, the fberendoscope
was introduced through the mouth-pharynges and let it to be
swallowed (Fig. 3c,d) through the Esophagic-Cardiac passage.
Previous protection of the endoscope was done with a curve
tube externally to the endoscope, mouth restraining and a
dental mouth opener to protect the unit.
Results and discussion
Videoendoscopy technique proved to be very useful
tool to evaluate gastrointestinal tract. Stomachs from fatter pigs
showed 95% PGDU prevalence, 92% in weaned piglets and
20% in newborns. as well as in weaned. The degree of PGDUS
ranged from mild (Grade I) to severe chronic-active (Grade IV)
more frequent in fatters. Many factors are associated with the
incidence of GDUS in pigs. Diet is one of them. However Table
I showed the positiveness to DNA of PCV2 extracted from
the GDF in the three cathegories studied. Helicobacter spp.
DNA extractions from pigs with GDUS have been previously
assessed by our research team (2). Likewise previous report
by using saliva was done to assessed PCV2 DNA extraction in
fatters with PNDs in deep bedding system (3). , report at the
IPVS 2008, DURBAN Bermudez etal.,South Africa).
Table I. Prevalence of Porcine Gastric-Duodenal Ulcers
Syndrome (GDUS) in weaned and fatters pigs.
Pigs GDUS DNA Helicobacter PCV2/ rtPCR
Newborns = 50 20% - +
Weaned = 50 92% + +
Fatters = 50 95% + +
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Acknowledgements
This work was funded by Consejo de Desarrollo
Cientfco y Humanstico de la Universidad Central de
Venezuela PG # 11.00.6631.2007.
References
1. Bermdez et al. (2000). Proc. IPVS 2000, p.34.
2. Morales, A. et al., Proc. IPVS, 2008, 0709.
3. Bermdez et al. (2008). Proc. IPVS 2008.

P.053
VIDEOENDOSCOPY OF PORCINE GASTRO-DUODENAL ULCER SYNDROME (PGDUS) IN SWINES TO STUDY VIRUS
BACTERIA INTERACTION
V.M. Bermdez G., M.M. Solorzano, A. Morales, J.M. Moreno, M. Devera, O. La Touch, E. Kwiecien.
Universidad Central de Venezuela, Veterinaria, Venezuela.