Question 1

The production of L-aspartic acid

The reaction of fumaric acid to form L-aspartic acid is an enzymatic conversion carried out using
the aspartase activity activity of bacteria Escherichia coli (E.coli) cells.

The production of L-phenylalanine
L-phenylalanine is produced via fermentation using a mutant Brevibacterium lactofermentum.
To achieve the production of L-aspartic acid and L-phenylalanine, a fermentation process need to
undergo to grow the bacteria in the presence of appropriate nutrients. The reaction of inoculation and
nutrient feed will fermented to produce fumaric acid. Then the fumaric acid will react with ammonia to
produce aspartic acid. The production of aspartic acid will react with elutant to form L-phenylalanine.

Question 2
There is one bypass stream which is at stream 28.From F-905(amino acid crystal filter) the excess of
amino acid that not crystallized were recycle back to stream 28 to undergo the process crystallization
again. In addition, there is no recycle loop involve in this production.

Question 3
Reactant to produce Aspartic acid
Aspartic acid product
Reactant to produce L-phenylalanine
L-phenylalanine acid product
Bypass stream


Reactant to produce Aspartic acid: Tactic 1 is applied to each unit operation in succession.
a) The inoculation feed of stream 1 enter F-901and stream 2 goes to fermenter R-901.
b) Ammonia feed of stream 7 and stream 8 enter fermenter R-901.
Aspartic acid product:
i. Tactic 2 is applied to each unit operation in succession.
a) Product stream 11 leaves fermenter R-901 and goes to bacteria filter F-904.
b) Product of stream 12 leaves bacteria filter F-904. It contains all the aspartic acid product.
ii. Tactic 1 is applied to each unit operation in succession.
c) Entering bacteria filter F-904 is stream 13 from fermenter R-901.It contains all the aspartic acid
product.
d) Entering slurry storage V-901 is stream 15 from bacteria filter F-904. It contains all the aspartic acid
product.
Reactant to produce L-phenylalanine: Tactic 1 is applied to each unit operation in succession.
a) Elutant feed of stream 17 enter ion exchange column T-901.
L-phenylalanine acid product: Tactic 2 is applied to each unit operation in succession.
a) Leaving ion exchange column T-901 is stream 19.
b) Entering product crystallizer CR-901 is stream 20 from the ion exchange column T-901 and amino acid
crystal filter F-905.
c) Entering amino acid crystal filter F-905 is stream 26 from product crystallizer CR-901.
d) Product of stream 27 leaves amino acid crystal filter F-905. It contains all the L-phenylalanine acid
product.
e) Apply tactic 5 at stream 28,bypass stream.