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Cultivation of Algae in Photobioreactor
Algae cultivation can be achieved in two ways: open ponds and photobioreactors (PBR). A photobioreactor is a closed
equipment which provides a controlled environment and enables high productivity of algae. As it is a closed system, all
growth requirements of algae are introduced into the system and controlled according to the requirements. PBRs
facilitate better control of culture environment such as carbon dioxide supply, water supply, optimal temperature, efficient
exposure to light, culture density, pH levels, gas supply rate, mixing regime, etc.,
Photobioreactor - How it works
Working of a photobioreactor:
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Flow description:
1. From the feeding vessel, the flow progresses to the diaphragm pump which moderates the flow of the algae into the
actual tube. Built into the pump is the CO2 inlet valve.
2. The photobioreactor itself is used to promote biological growth by controlling environmental parameters including light.
The tubes are made of acrylic and are designed to have light and dark intervals to enhance the growth rate.
3. The photobioreactor has a built-in cleaning system that internally cleans the tubes without stopping the production.
4. After the algae have completed the flow through the photobioreactor, it passes back to the feeding vessel. As it
progresses through the hoses, the oxygen sensors determine how much oxygen has built up in the plant and this oxygen
is released in the feeding vessel itself. It is also at this stage that the optical Cell Density sensor determines the
harvesting rate.
5. When the algae are ready for harvesting, they pass through the connected filtering system. This filter collects the algae
that are ready for processing, while the remaining algae passes back to the feeding vessel.
6. The flow continues.
Advantages of Photobioreactors
Cultivation of algae is in controlled circumstances, hence potential for much higher productivity
Large surface-to-volume ratio. PBRs offer maximum efficiency in using light and therefore greatly improve
productivity. Typically the culture density of algae produced is 10 to 20 times greater than bag culture in which
algaeculture is done in bags - and can be even greater.
Better control of gas transfer.
Reduction in evaporation of growth medium.
More uniform temperature.
Better protection from outside contamination.
Space saving - Can be mounted vertically, horizontally or at an angle, indoors or outdoors.
Reduced Fouling - Recently available tube self cleaning mechanisms can dramatically reduce fouling.
Covering ponds does offer some of the benefits that are offered by photobioreactors, but enclosed systems will still provide
better control of temperature, light intensity, better control of gas transfer, and larger surface area-to-volume ratio. An
enclosed PBR design will enhance commercial algal biomass production by keeping algae genetics pure and reducing the
possibility of parasite infestation.
Disadvantages of Photobioreactors
Capital cost is very high. This is one of the most important bottlenecks that is hindering the progress of algae fuel
industry.
Despite higher biomass concentration and better control of culture parameters, data accumulated in the last two
decades have shown that the productivity and production cost in some enclosed photobioreactor systems are not
much better than those achievable in open-pond cultures.
The technical difficulty in sterilizing these photobioreactors has hindered their application for algae culture for
specific end-products such as high value pharmaceutical products.
General Specifications of a Photobioreactor
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Data i n the tabl e are for the Aquasearch-coupl ed producti on system for photosyntheti c mi crobes from HR Bi opetrol eum. Source: HR Bi opetrol eum
Requirements to develop a high-performance photobioreactor for algal cultivation:
1. In order to attain high productivity, the volume of the non-illuminated parts of the reactor should be minimized.
2. In order to ensure a high efficiency of light use by the culture, the design must provide for the uniform illumination of
the culture surface and the fast mass transfer of CO
2
and O
2
.
3. To prevent rapid fouling of light-transmitting surfaces of reactors, photobioreactors must be frequently shut down for
their mechanical cleaning and sterilization
4. High rates of mass transfer must be attained by means that neither damage cultured cells nor suppress their
growth.
5. For the industrial-scale production of biomass, the energy consumption required for mass transfer and the
arrangement of the light-receiving surface of the algal suspension must be reduced to its minimum possible.
PBRs are complex systems composed of several subsystems. The key systems are:
Light system
Optical transmission system
Air handling & gas exchange systems
Mixing system
Nutrient system
Instrumentation system
Electrical system
Some of the key sub-components of the above system are:
Oxygen & CO2 sensors
Temperature sensor
pH sensor
Light sensor
Conductivity sensor
Recirculation pump
Harvest pump
CO2 injection valve
Substrate pump
Filtrate recirculation valve
Water inlet valve purge valve
Connectors and hoses
Oxygen release system
PLC control panel
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Feeding tank
Several of the subsystems of a PBR interact. For instance, the optical transmission system and gas exchange system
interact via the mixing that takes place in the reaction area.
To know more about Photobioreactors, buy our Comprehensive Oilgae report with its
recent updated version. List of contents under this topic include
1. Concepts
2. Types of Bioreactors Used for Algae Cultivation
3. Parts & Components
4. Design Principles
5. Costs
6. PBR Manufacturers & Suppliers
7. Photobioreactors Q&A
8. Research Done on Bioreactors and Photobioreactors
9. Challenges & Efforts in Photobioreactor
10. Photobioreactor Updates and Factoids
11. Useful Resource
Related Links:
Microalgal Photobioreactors: Scale - up and Optimization
Photobioreactor Technology for Microalgae Cultivation
Closed Photobioreactors for Microalgal Cultivation
Photobioreactors for Cultivation of Microalgae under Strong Irradiances: Modelling, Simulation and Design
High-Density Algal Photobioreactors Using Light-Emitting Diodes
Materials, geometry, and net energy ratio of tubular photobioreactors for microalgal hydrogen production
Algae Photobioreactor Design Considerations for Commercial Scale Production
https://mospace.umsystem.edu/xmlui/bitstream/handle/10355/956/Algae%20Photobioreactor%20Design%20[abstract].pdf?
sequence=3
Study of light requirements of a Photobioreactor
Efficient nutrient removal from swine manure in a tubular biofilm photo-bioreactor using algae-bacteria consortia
A New Photobioreactor for Continuous Microalgal Production in Hatcheries Based on External-Loop Airlift and Swirling
Flow
Microalgae Grown in Photobioreactors for Mass Production of Biofuel
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Algae to Ethanol?
Related Forum Discussions
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Can sugar replace light in PBR's?
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Yield?
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Algae will make you
Live Longer
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