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STUDENT ID
14WAR08925
14WAR10521
GROUP: RBS 2 A1
DATE: 2 JULY 2014
DEMONSTRATER: DR LOH KHYE ER
Objectives:
1. To determine the effect of colchicine on onion root tips.
Introduction:
Mitosis
Mitosis is the nuclear division of a eukaryotic cell which produces two daughter
cells identical to the dividing parent cell. Mitosis is important for growth in our human
body by replacing old cells and tissue repair. Mitosis also is part of asexual reproduction
for single celled and some multicellular eukaryotes. Mitosis is a one portion of the cell
cycle. One complete cell cycle is divided into interphase, mitosis and cytoplasmic
division in eukaryotic cells. Most of the time, the cell are in interphase, which during the
cell synthesizes nuclear components and cytoplasmic.
DNA replication also occurs in interphase. This process involves the precise
duplication of the entire DNA in the nucleus, in preparation for mitosis. Mitosis creates
two identical nuclei, one for each daughter cell in the physical division of the nucleus.
Cytoplasmic division results in the partitioning of the cytoplasmic components and the
physical separation of the two new daughter cells.
Mitosis has four basic stages; they are prophase, metaphase, anaphase
and telophase. Prophase is where the replicated chromosomes condense into compact
rods. Each rod consists of two sister chromatids joined at the centromere. The spindle
apparatus made of microtubules and centrioles at opposite poles of the cell. Microtubules
attached at the centromere of each chromosome and then connect the chromosomes to the
spindle apparatus. The nuclear membrane breaks up within nucleus during these changes.
In metaphase, the chromosomes are in their most condensed form, line up at the spindle
equator. For anaphase stage, the two sister chromatids break their attachment to each
other. As separate chromosomes, they are pulled to opposite spindle poles by the
microtubules. The last stage is telophase. Now, the cell has two clusters of chromosomes,
which are located at opposite poles. A new nuclear membrane begins to form around each
of these clusters. This is resulting in two new nuclei, one for each new daughter cell. The
DNA replication during interphase is to ensure that each daughter cell receives a full
chromosomal complement of 46 chromosomes. Cytoplasmic division is started at this
point in mitosis. In plants, a cell plate is formed by vesicles filled with cell wall
material. These vesicles combine at the former site of the spindle equator. Cellulose
deposits accumulate at this plate. Eventually, a new cell wall is formed and separating the
parent cell into two daughter cells.
The Role of Spindle Fibers in Mitosis
Spindle fibers play an important role in mitotic process due to them contains a
highly organized array of microtublules, which composed of globular proteins in long,
hollow, unbranched tubes. Microtubules are components of other cellular structures,
including cytoskeleton. They form the core of both cilia and flagella. Besides involving in
separating chromosomes during mitosis, microtubules are structural supports and
organizers for cells. In plant cells, it maintains cell shape through their influence on the
formation of the cell wall during interphase. Apart from that, microtubules also maintain
internal organization of cells and are responsible for intracellular motility of
Watch glass was warmed by over a spirit lamp until white vapors were seen (~1 min)
The watch glass was closed with another watch glass and allowed it to cool for about 5 to
10 mins.
The tip of the root (~ 1min) was cut and discarded the rest.
1 or 2 drops of 1% aceto orcein were placed on the glass slide with the tip of root.
The glass rod was used to tap gently on the root tips so that the cells get separated.
The excess stain was blotted by tissue and without removing the coverslip.
The slide was inverted carefully onto a filter paper and pressed gently with thumb
without shifting the slide.
(II)
References:
1. Alberts, Bruce, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts and
Peter Walter. Molecular Biology of the Cell. 5th ed. New York: Garland Science,
Taylor and Francis Group, LLC, 2008.
2. Brooker, Robert. Genetics: Analysis and Principles. New York: McGraw-Hill Publishing, 2005.
3. Lodish, Harvey, Arnold Berk, S. Lawrence Zipursky, Paul Matsudaira, David
Baltimore and James Darnell.Molecular Cell Biology. 4th ed. New York: WH
Freeman and Company, 2000.
4. Starr, Cecie and Ralph Taggart. Cell Biology and Genetics. California: Thomson
Brooks/Cole, 2006.