Bio-Rad

Laboratories Life Science Group

2000 Alfred Nobel Drive
Hercules, California 94547
Telephone: 510-741-1000
Facsimile: 510-741-5800

Gene Pulser Xcell™ Electroporation System

Solo Source Specifications

Choice of Exponential or Square Waveform

The Gene Pulser Xcell system generates both exponential and square waveforms enabling the
customer to choose the waveform and protocol that will work best for their cells. It is the only
system available that can produce both waveforms. Both exponential and square waves have been
used very effectively for both electroporation and electrofusion. The shape of the electroporation
wave can have a significant effect on the transformation efficiency for t different cell types.

Pulse Trac circuitry and Arc Protection provide Reliable, Reproducible and safe
Performance

Bio-Rad’s unique, patented microprocessor controlled circuitry delivers reproducible results for
any protocol, giving the user confidence that the voltage delivered values can be tested and
repeated worldwide, regardless of the media used. Pulse Trac and Arc Protection:

• Facilitate user-related capacitor re calibration to maintain accurate pulse specification,

correcting for capacitor drift that occurs overtime
• Provide prepulse sample resistance measurement
• Tighten the already rigorous precision of the low-voltage capacitors in the CE module
from 20 to 10%
• Enable safe, automatic discharge of current if the pulse or circuit is interrupted
• Reduce the risk of arcing in the high-voltage circuit, protecting both instrument and
sample.

Modular System

The Gene Pulser Xcell is available in a choice of systems allowing the purchase of components
appropriate for the application being considered and permitting future upgrades for long-term
value.
User-friendly Interface

East programming and complete reporting of results make each electroporation simple to perform.
The graphical interface on the main unit enables east programming and controls all functions,
including those of any connected accessory modules. The interface consists of a single screen used
with function keys and an alphanumeric keypad. Programming is simple and intuitive using on-
screen prompts. The screen is used for programming, to display stored and set programs,
parameters delivered and a graphical display of the waveform.

Pre-optimized programs for rapid programs selection of commonly used microbial

and mammalian cells.

This program proposes a specific and pre-optimized set of parameters for electro-transformation of
commonly used bacterial, fungal, and mammalian cells.

User Method Storage for quick access to favorite methods

The method storage program enables 12 users to each store 12 programs, a total of 144 protocols
including all instrument settings. The program can be saved by the name using the alphanumeric
keypad and recalled to deliver a pulse.

Optimization Protocol for easy determination of the optimal pulse parameters

Optimal conditions need to be determined for each new experimental system by using a series of
preliminary experiments to determine the optimal pulse parameters. The optimization protocol
enables experiments to be carried out by increasing or decreasing the voltage.

Data Management

The data management program enables user to access pulse parameters and results for the last 100
pulses logged by date and time.

ShockPod™ Shocking Chamber designed for one-handed use to allow easier and
more rapid sample handling

The new innovative Shockpad enables rapid and easy one-handed operation with a single cuvette.
A simple catch on the front of the pod open the lid. The cuvette slot is designed so that the cuvette
may only be inserted in the proper orientation.
The ShockPod has a safety interlock that prevents a pulse being delivered to the cuvette when the
ShockPod lid is open. (Although the instrument will still indicate that a pulse has been discharged,
this is done safely within the system.) Additionally, the top must also be closed to use the
ShockPod to measure resistance of electroporation media.

Meets all safety requirements

The Gene Pulser Xcell is designed and certified (in any configuration) to meet the safety
requirement of EN61010 and the EMC requirements of EN61326 (for Class A) and conforms to
the “Class A” standards for electromagnetic emissions intended for laboratory equipment
applications. EN61010, which covers European low-voltage Directive for electrical safety and
electromagnetic emission levels. Products carrying the CE mark not only satisfy EN61010 but also
European EMC Directive for electromagnetic susceptibility.