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According to this theory life originated on early earth through physico-chemical processes of atoms combining to form molecules, molecules in turn reacting to produce
inorganic and organic compounds. Organic compounds interacted to produce all types
of macromolecules which organised to form the first living system or cells.
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Modem views regarding the origin of life include chemical evolution and biological
evolution:
A. Chemical Evolution (Chemogeny):
1. The Atomic Phase:
Early earth had innumerable atoms of all those elements (e.g., hydrogen, oxygen,
carbon, nitrogen, sulphur, phosphorus, etc.) which are essential for the formation of
protoplasm. Atoms were segregated in three concentric masses according to their
weights, (a) The heaviest atoms of iron, nickel, copper, etc. were found in the centre of
the earth, (b) Medium weight atoms of sodium, potassium, silicon, magnesium, aluminium, phosphorus, chlorine, fluorine, sulphur, etc. were collected in the core of the
earth, (c) The lightest atoms of nitrogen, hydrogen, oxygen, carbon etc. formed the
primitive atmosphere.
2. Formation of Inorganic Molecules:
Free atoms combined to form inorganic molecules such as H2(Hydrogen),
N2 (Nitrogen), H20 (Water vapour), CH4 (Methane), NH3 (Ammonia), C02 (Carbon
dioxide). Hydrogen atoms were most numerous and most reactive in primitive
atmosphere.
First hydrogen atoms combined with all oxygen atoms to form water and leaving no free
oxygen. Thus primitive atmosphere was reducing atmosphere (without free oxygen)
unlike the present oxidising atmosphere (with free oxygen).
Hydrogen atoms also combined with nitrogen, forming ammonia (NH3). So water and
ammonia were probably the first molecules of primitive earth.
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Torrential rains must have fallen. As the water rushed down, it must have dissolved
away and carried with it salts and minerals, and ultimately accumulated in the form of
oceans. Thus ancient oceanic water contained large amounts of dissolved NH3, CH4,
HCN, nitrides, carbides, various gases and elements.
CH4 + C02 + H20 > Sugars + Glycerol + Fatty Acids
CH4 + HCN + NH3 + H20 > Purines + Pyrimidines
CH4 + NH3 + C02 + H20 > Amino Acids
Some external sources must have been acting on the mixture for reactions. These
external sources might be (i) solar radiations such as ultra-violet light, X-rays, etc., (ii)
energy from electrical discharges like lightning, (iii) high energy radiations are other
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sources of energies (probably unstable isotopes on the primitive earth). There was no
ozone layer in the atmosphere.
A soup-like broth of chemicals formed in oceans of the early earth from which living
cells are believed to have appeared, was termed by J.B. Haldane (1920) as prebiotic
soup (also called hot dilute soup). Thus the stage was set for combination of various
chemical elements. Once formed, the organic molecules accumulated in water because
their degradation was extremely slow in the absence of any life or enzyme catalysts.
Experimental Evidence for Abiogenic Molecular Evolution of Life:
Stanley Miller in 1953, who was then a graduate student of Harold Urey (1893-1981) at
the University of Chicago, demonstrated it clearly that ultra-violet radiation or electrical
discharges or heat or a combination of these can produce complex organic compounds
from a mixture of methane, ammonia, water (stream of water), and hydrogen. The ratio
of methane, ammonia and hydrogen in Millers experiment was 2:1:2.
Miller circulated four gases methane, ammonia, hydrogen and water vapour in an air
tight apparatus and passed electrical discharges from electrodes at 800C. He passed
the mixture through a condenser.
He circulated the gases continuously in this way for one week and then analysed the
chemical composition of the liquid inside the apparatus. He found a large number of
Modern Theory on the Origin of Life
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simple organic compounds including some amino acids such as alanine, glycine and
aspartic acid. Miller conducted the experiment to test the idea that organic molecules
could be synthesized in a reducing environment.
Other substances, such as urea, hydrogen cyanide, lactic acid and acetic acid were also
present. In another experiment Miller circulated the mixture of the gases in the same
way but he did not pass the electric discharge. He could not get the significant yield of
the organic compounds.
Later on many investigators have synthesized a great variety of organic compounds
including purines, pyrimidines and simple sugars, etc. It is considered that the essential
building blocks such as nucleotides, amino acids, etc. of living organisms could thus
have formed on the primitive earth.
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S.W. Fox of the University of Miami has demonstrated that if a nearly dry mixture of
amino acids is heated, polypeptide molecules are synthesized. Similarly simple sugars
could form polysaccharides and fatty acids could combine to produce fats. Amino acids
could form proteins, when other factors were involved.
Thus the small simple organic molecules combined to form large complex organic
molecules, e.g., amino acid units joined to form polypeptides and proteins, simple sugar
units combined to form polysaccharides, fatty acids and glycerol united to form fats,
sugars, nitrogenous bases, and phosphates combined into nucleotides which polymerized into nucleic acids in the ancient oceans.
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(c) The system of replicators must have required a continuous supply of free energy and
partial isolation from the general environment.
The high temperature in early earth would have fulfilled the requirement of mutation.
1. Protobionts or Protocells:
These are at least two types of fairly simple laboratory produced structures Oparins
coacervates and Foxs microspheres which possess some of the basic prerequisites of
proto cells.
Although these structures were created artificially, they point to the likelihood that nonbiological membrane enclosures (proto cells) could have sustained reactive systems for
at least short periods of time and led to research on the experimental production of
membrane bound vesicles containing molecules, i.e., proto cells.
(i) Coacervates:
The first hypothesis was proposed by Oparin (1920). According to this hypothesis early
proto cell could have been a coacervate. Oparin gave the term coacervates. These were
non-living structures that led to the formation of the first living cells from which the
more complex cells have today evolved.
Oparin speculated that a proto cell consisted a carbohydrates, proteins, lipids and
nucleic acids that accumulated to form a coacervate. Such a structure could have
consisted of a collection of organic macromolecules surrounded by a film of water
molecules.
This arrangement of water molecules, although not a membrane, could have functioned
as a physical barrier between the organic molecules and their surroundings. They could
selectively take in materials from their surroundings and incorporate them into their
structure.
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Coacervates have been synthesized in the laboratory. They can selectively absorb
chemicals from the surrounding water and incorporate them into their structure. Some
coacervates contain enzymes that direct a specific type of chemical reaction.
Because they lack a definite membrane, no one claims coacervates are alive, but they do
exhibit some life like characters. They have a simple but persistent organization. They
can remain in solution for extended periods. They have the ability to increase in size.
(ii) Microspheres:
An another hypothesis is that early proto cell could have been a microsphere. A
microsphere is a non-living collection of organic macromolecules with double layered
outer boundary. The term microsphere was given by Sydney Fox (1958-1964).
Sidney Fox demonstrated the ability to build microspheres from proteinoids.
Proteinoids are protein like structures consisting of branched chains of amino acids.
Proteinoids are formed by the dehydration synthesis of amino acids at a temperature of
180C. Fox, from the University of Miami, showed that it is feasible to combine single
amino acids into polymers of proteinoids. He also demonstrated the ability to build
microspheres from these proteinoids.
Fox observed small spherical cell-like units that had arisen from aggregations of
proteinoids. These molecular aggregates were called proteinoid microspheres. The first
non-cellular forms of life could have originated 3 billion years back. They would have
been giant molecules (RNA, Proteins, Polysaccharides etc.).
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Microspheres can be formed when proteinoids are placed in boiling water and slowly
allowed to cool. Some of the proteinoid material produces a double-boundary structure
that encloses the microsphere. Although these walls do not contain lipids, they do
exhibit some membrane like characteristics and suggest the structure of a cellular
membrane.
Microspheres swell or shrink depending on the osmotic potential in the surrounding
solution. They also display a type of internal movement (streaming) similar to that
exhibited by cells and contain some proteinoids that function as enzymes. Using ATP as
a source of energy, microspheres can direct the formation of polypeptides and nucleic
acids. They can absorb material from the surrounding medium.
They have the ability of motility, growth, binary fission into two particles and a capacity
of reproduction by budding and fragmentation. Superficially, their budding resembles
with those of bacteria and fungi.
According to some investigators, microspheres can be considered first living cells.
2. Origin of Prokaryotes:
Prokaryotes were originated from proto cells about 3.5 billion years ago in the sea. The
atmosphere was anaerobic because free oxygen was absent in the atmosphere.
Prokaryotes do not have nuclear membrane, cytoskeleton or complex organelles. They
divide by binary fission. Some of the oldest known fossil cells appear as parts of
stromatolites. Stromatolites are formed today from sediments and photosynthetic
prokaryotes (mainly filamentous cynobacteria blue green algae).
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