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Culture Documents
INTRODUCTION
Medicinal plants are part and parcel of human society to
combat diseases from the dawn of civilization. India has
an ancient heritage of traditional systems of medicine
such as Ayurveda, Siddha and Unani. According to
World Health Organization, 80% of the population of
developing countries are unable to afford pharmaceutical
drugs. They rely on plant based traditional medicines for
their primary health care needs1.
The plant Stachytarpheta jamaicensis belongs to the
family Verbenaceae. It is an erect or struggling
perennial herb of about 60-90cm in height. The stem is
smooth and somewhat woody especially at the base. The
leaves are opposite and whorled, ovate or oblong, elliptic
and between 4-11 cm long and 2-4.5cm wide, rounded to
broadly acute at the apex, widely toothed at the margins,
smooth on both surfaces with short petioles. The flowers
are bluish with a white threat and a tubular corolla about
10mm long and lobes about 3mm long. They are
sparsely grouped along and immersed in the axis of the
inflorescence2.
Ethnobotanically Stachytarpheta jamaicensis is used as
antacid, analgesic, antihelmintic, anti-inflammatory,
antispasmodic, antiulcerogenic, diuretic, hypotensive,
spasmodic, laxative, lactogogue, purgative, sedative and
Page 234-239
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8.
S. No.
Organism
Escherichia coli
(MTCC 1675)
Staphylococcus aureus
(MTCC 7505 )
Enterococcus faecalis
(MTCC 459)
Klebsiella pneumonia
(MTCC 7028)
Bacillus subtilis
( MTCC 2757)
Pseudomonas aeruginosa
(MTCC2495)
Salmonella typhi
(MTCC 733)
1
2
3
4
5
6
7
Zone diameter in mm
5
2.5
mg/ml
mg/ml
20
mg/ml
10
mg/ml
1.25
mg/ml
Std
18
16
15
13
10
17
19
18
15
13
10
22
14
13
12
11
18
Nil
Nil
Nil
Nil
Nil
18
17
15
14
12
11
24
Nil
Nil
Nil
Nil
Nil
17
14
13
11
10
16
S.
No.
1
2
3
4
5
6
7
Organism
Escherichia coli
(MTCC 1675)
Staphylococcus aureus
(MTCC 7505 )
Enterococcus feacalis
(MTCC 459)
Klebsiella pneumonia
(MTCC 7028)
Bacillus subtilis
(MTCC 2757)
Pseudomonas aeruginosa
(MTCC2495)
Salmonella typhi
(MTCC 733)
Zone diameter in mm
5
2.5
mg/ml
mg/ml
20
mg/ml
10
mg/ml
1.25
mg/ml
Std
14
13
10
17
15
14
12
Nil
22
Nil
Nil
Nil
Nil
Nil
18
Nil
Nil
Nil
Nil
Nil
18
16
14
13
12
24
Nil
Nil
Nil
Nil
Nil
17
15
14
13
10
16
Page 234-239
S.
No.
1
2
3
4
Organism
Aspergillus niger (MTCC 1344)
Aspergillus fumigatus (MTCC 3377)
Candida albicans (MTCC 7253)
Saccharomyces cerevisiae (MTCC 823)
Zone diameter in mm
20mg/
10mg/ml
5mg/ml
ml
Nil
Nil
Nil
Nil
Nil
Nil
15
14
12
16
13
12
Std
19
17
19
18
Organism
Ext
20
mg/ml
10
mg/ml
5
mg/ml
2.5
mg/ml
Activity
1.25
mg/ml
0.625
mg/ml
0.312
mg/ml
0.156
mg/ml
0.078
mg/ml
0.039
mg/ml
Escherichia coli
(MTCC 1675)
CE
AE
Staphylococcus aureus
(MTCC 7505 )
CE
AE
Enterococcus faecalis
(MTCC 459)
CE
AE
Bacillus subtilis
(MTCC 2757)
CE
AE
Salmonella typhi
(MTCC 733)
CE
AE
Organism
Ext
20
mg/ml
10
mg/ml
5
mg/ml
2.5
mg/ml
1.25
mg/ml
0.625
mg/ml
0.312
mg/ml
0.156
mg/ml
Candida albicans
(MTCC 7253)
CE
Saccharomyces cerevisiae
(MTCC 823)
CE
AE: Alcohol extract, CE: Chloroform extract (+) --- presence of growth,
The results are the average of the triplicates
Test
Alkaloid
Amino acids
Coumarin
Flavone
Glycoside/Sugar
Phenol
Quinone
Steroid
Tannin
Terpenoid
Carboxylic acids
Furanoids
Saponins
n-Hexane
Chloroform
Ethanol
+
+
-
+
+
+
+
+
-
+
+
+
+
+
+
+
+
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Chart: 1
Chart: 2
Chart: 3
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