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Vivapure AdenoPACK 20
Adenovirus (Ad5) purification and concentration kit for up to 20 ml cell culture
volume (E.g. 1 +15 cm plate)
85030-523-69
Vivapure AdenoPACK 20
Cat. Number
Number of purifications possible
with AdenoPACK 20
AdenoPACK Maxi spin columns
Vivaclear Maxi 0.45 m PES
Empty 50 ml tubes
Loading Buffer (10 +)
Washing Buffer (10+)
Elution Buffer
Benzonase * (12.5 U/l)
Vivaspin 20, 100 kDa MWCO
Technical data sheet
Materials of construction
AdenoPACK Maxi spin column
housing
Vivaclear Maxi housing
AdenoPACK membrane
Buffer containers
Purification buffers
Kit specifications
Sample size
Virus particles (VP) per ml
VP/IU
Processing time
Endotoxin level
VS-AVPQ020
6+20 ml
6
6
6
25 ml
30 ml
20 ml
120 l
6
1 each for kit and Vivaspin
Polypropylene
Polypropylene
Stabilised RC
LDPE
Proprietary
20 ml of Adenovirus
supernatants
Typically up to 1+10111012
50100
Typically 1 hour
<0.025 EU/ml
Kit contents
Yellow
Loading
Buffer 10+
1+ 25 ml Loading Buffer
6+ Vivaspin 20 concentrators
with 100 kDa membrane
Washing
Buffer 10+
1+ 30 ml Washing Buffer
Elution
Buffer
1+ 20 ml Elution Buffer
Re-suspended pellet
Reserved supernatant
Debris
Discard
3
4. . Ad
Eq d
ui 10
lib +
ra Lo
te ad
Ad in
en g B
oP u
AC ffe
K r to
M s
ax am
is p
pi le
n
co
lu
m
ns
1. Freeze|thaw 3 times
2. Centrifuge to pellet debris
3. Re-combine with reserved supernatant
Viral supernatant
spin
1. Treat with
Benzonase
2. Filter with
Vivaclear Maxi
spin
5. Bind virus
and wash away
contaminants
spin
6. Elute
purified virus
Final
concentration|
buffer
exchange
! Caution:
Do not reduce the volume
to less than 200 l in order
to avoid aggregation and
loss of infectivity.
General information
Typical performance
For a normal yielding vector,
1+15 cm culture plates purified
using this method should yield a
range of up to 1 +1012 viral particles (see table 1).
Usage tips
It is recommended that virus
is exchanged into normal
physiological buffer before
use in tissue culture or cell
based assays.
Table1: Purification results from preparations with certain Ad5 GFPconstructs depending on individual conditions values may be different.
Purification
method
20 ml culture
500 ml CsCl
Process
time
1 hour
1248 hours
Eluate
Recovery**
1 ml
12* ml
6570%
6070%
Viral
particles
1+1011-12
1+1011-12
* after dialysis
** before buffer exchange
Ordering information
Ordering Information
VS-AVPQ020
Vivapure AdenoPACK 20, 20 ml culture volume
VS-AVPQ022
Vivapure AdenoPACK 20 RT, 20 ml culture volume*
VS-AVPQ101
Vivapure AdenoPACK 100, 200 ml culture volume
VS-AVPQ102
Vivapure AdenoPACK 100 RT, 200 ml culture volume*
VS-AVPQ501
Vivapure AdenoPACK 500, 500 ml culture volume
VS-AVPQ502
Vivapure AdenoPACK 500RT, 500 ml culture volume*
Pack Size
6
6
1
1
1
1
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