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Description

NucleicAcidProductSheet

Geobactermetallireducens
Lovleyetal.(ATCC
53774D5)
PleasereadthisFIRST

Source:GeobactermetallireducensLovleyetal.
Designation:GenomicDNAfromstrainGS15[ATCC53774]
Description:
GenomicDNAisolatedfromGeobactermetallireducensstrainGS15.Thisbacterialstrainisalsoavailableas
ATCCCatalogNo.53774.
Note:
GenomicDNAisolatedfrombacteriaisappropriateforPCR*andothermolecularbiologyapplications.
*Thepolymerasechainreaction(PCR)processiscoveredbypatentsownedbyHoffmannLaRocheInc.Use
ofthePCRprocessrequiresalicense.

BatchSpecificInformation
RefertotheCertificateofAnalysisforbatchspecifictestresults.

BiosafetyLevel
1

IntendedUse
Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.

CitationofStrain
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:GeobactermetallireducensLovleyetal.
(ATCC53774D5)

NucleicAcidInformation

QualityControlInformation
1. BacterialgenomicDNAisprovidedinadriedform.Storeat2Cto8Cuponreceipt.Storeat20Cif
storedformorethan6months.Note:Donotstoreinfreezerswithadefrostcycle.Thiswillexpose
theproducttoincreasedtemperatures.
2. TotalDNAbyPicoGreenmeasurementwasfoundtobeapproximately5g.
3. Purity:OD260/OD280ratio.
4. IntegrityofDNAwasdeterminedbyelectrophoresisona1%agarosegelstainedwithSYBRSafe,
andwasfoundtobeofhighmolecularweight.
5. NoRNAwasdetectedbyelectrophoresis.
6. FunctionalactivitywasconfirmedbyPCRamplification16SribosomalRNAgene.
7. Identityconfirmedbysequencingof16SribosomalRNAgene(first~500basepairs).

BiosafetyLevel:1
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Laboratorysafetyisdiscussedin
thecurrentpublicationoftheBiosafetyinMicrobiologicalandBiomedicalLaboratoriesfromtheU.S.
DepartmentofHealthandHumanServicesCentersforDiseaseControlandPreventionandNationalInstitutes
forHealth.

Atleast5gin1XTEbuffer

ATCCWarranty
TheviabilityofATCCproductsiswarrantedfor30daysfromthedateofshipment,andisvalidonlyifthe
productisstoredandculturedaccordingtotheinformationincludedonthisproductinformationsheet.ATCC
liststhemediaformulationthathasbeenfoundtobeeffectiveforthisstrain.Whileother,unspecifiedmedia
mayalsoproducesatisfactoryresults,achangeinmediaortheabsenceofanadditivefromtheATCC
recommendedmediamayaffectrecovery,growthand/orfunctionofthisstrain.Ifanalternativemedium
formulationisused,theATCCwarrantyforviabilityisnolongervalid.

Disclaimers

AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org
800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

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Thisproductisintendedforlaboratoryresearchpurposesonly.Itisnotintendedforuseinhumans.
WhileATCCusesreasonableeffortstoincludeaccurateanduptodateinformationonthisproduct
sheet,ATCCmakesnowarrantiesorrepresentationsastoitsaccuracy.Citationsfromscientificliterature
andpatentsareprovidedforinformationalpurposesonly.ATCCdoesnotwarrantthatsuchinformationhas
beenconfirmedtobeaccurate.
Thisproductissentwiththeconditionthatyouareresponsibleforitssafestorage,handling,anduse.
ATCCisnotliableforanydamagesorinjuriesarisingfromreceiptand/oruseofthisproduct.While
reasonableeffortismadetoinsureauthenticityandreliabilityofstrainsondeposit,ATCCisnotliablefor
damagesarisingfromthemisidentificationormisrepresentationofcultures.
PleaseseetheenclosedMaterialTransferAgreement(MTA)forfurtherdetailsregardingtheuseofthis
product.TheMTAisalsoavailableonourWebsiteatwww.atcc.org
AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.
ATCC2013.Allrightsreserved.ATCCisaregisteredtrademarkoftheAmericanTypeCultureCollection.[02/26]

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