Chemical Oxygen Demand (COD)

(Reaction Digestion Method)

1.0 Objective
To determine the chemical oxygen demand of the samples

2.0 Apparatus
1. Digestion reactor / COD reactor
2. Spectrophotometer
3. Pipette, 0.1-1ml +tips
4. COD vials rack

3.0 Reagents / Chemicals

1. COD digestion reagent vials
2. COD standard solution (HACH COD standard solution of 300 mg/l or 1000 mg/l)

4.0 Procedure
Digestion of sample:
1.100 ml of sample was been blended for 30 sec in order to get the homogenize sample.

2. The homogenized sample was poured into 250 ml beaker and been stirred by using a magnetic
stir plate.
3. The sample then was pipetted into 3 bottles of vial (each vial about 2.0 ml)
4. All vials were capped tightly and been inverted gently for several times to make sure that the
content was mixed. The exterior of all the COD were rinsed with deionised water and wiped by
using paper towel. The vials then were placed in the preheated COD reactor
5. A blank and standard solution were prepared by repeating steps 3-4.(but only 1 bottle of vial
for each solution)
6. The exterior wall of all the vials were wiped , cleaned ,before been heated simultaneously in
the digestion reactor for two hours.
7. The reactor was turn off and let it cool to about 120 Celsius
8. Each vial was inverted for several times while still warm
9. The process then was proceeded to colorimetric determination in order to measure COD.

5.0 Results
READING (mg/L)
A

average

SEK 2

75

166

71

104

U 12

50

19

47

116

SEK 7

173

155

108

145

Botanical

18

16

13

Sources of
water sample
(lake)

garden
Table 5.1: Spectrophotometers reading of water sample

Sources of water sample

Reading of

(lake)

Standard Solution (mg/L)

SEK 2

76

U 12

132

SEK 7

114

Botanical garden

11

Table 5.2: Spectrophotometers reading of standard solution

Parameters

Reading

Dissolved Oxygen (%)

19.2

Dissolved Oxygen (mg/L)

1.41

Temperature

31.58

Salinity

0.03

pH

10.66

Total Dissolved Solids (TDS %)

0.046

ORP

149.3
Table 5.3: YSIs reading meter at the U12