Professional Documents
Culture Documents
377-388
ISSN: 2222-2510
2011 WAP journal. www.waprogramming.com
Graphene:
Synthesis and Applications in Biotechnology - A Review
Mohammad Hakimi
Paransa Alimard
Chemistry Department,
Payame Noor University,
19395-4697 Tehran, Iran
mohakimi@yahoo.com
Chemistry Department
Payame Noor University
19395-4697 Tehran
Abstract: Graphene has emerged as an exotic material of the 21st century, and received world-wide attention
due to its exceptional charge transport, thermal, optical, and mechanical properties. However, the severe
chemical conditions required to prepare graphene from naturally occurring graphite has become the biggest
limiting factor for high scale graphene production and commercialization. Many bioapplications have been
proposed for this material. In this review various synthesis processes of single layer graphene are reviewed
and selectively current advances in the field of graphene bioapplications are analyzed.
Key word: Graphene, synthesis, bioapplication, biotechnology.
I.
INTRODUCTION
Graphene is a two-dimensional (2D) layer of carbon atoms ordered into a honeycomb lattice as shown in Fig. 1.
Graphene, one of the allotropes (carbon nanotube, fullerene, diamond) of elemental carbon, is a planar monolayer of
carbon atoms with a carboncarbon bond length of 0.142 nm [1]. Electrons in graphene behave like massless
relativistic particles, which contribute to very peculiar properties [211] such as dirac spectrum of low-lying
quasiparticles [3], large mean-free-path [4], and high electron mobility [12, 13].
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II.
SYNTHESIS OF GRAPHENE
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could insert into and increase the distance between adjacent layers of graphite facilitating the separation of graphene
sheets in surfactant solutions.
2.
3.
4.
Catalytic decomposition of methane on Cu to form CxHy upon the exposure of Cu to methane and hydrogen.
In this process, the Cu surface is either undersaturated, saturated, or supersaturated with CxHy species,
depending on the temperature, methane pressure, methane flow, and hydrogen partial pressure.
Formation of nuclei as a result of local supersaturation of CxHy where undersaturated Cu surface does not
form nuclear.
Nuclei grow to form graphene islands on Cu surface saturated, or supersaturated with CxHy species.
Full Cu surface coverage by graphene under certain temperature (T), methane flow rate (JMe), and methane
partial pressure (PMe).
An interesting feature of the CVD approach to synthesize graphene is the possibility for substitutional doping by
introducing other gases, such as NH3, during the growth [3537]. The nitrogen atoms can be doped into graphene as
pyridinic, graphitic and pyrrolic forms. These nitrogen doped graphene (N-graphene) layers have
demonstrated interesting properties.
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before processing devices [40,4143]. When SiC substrate is heated under UHV, silicon atoms sublimate from the
substrate. The removal of Si leaves surface carbon atoms to rearrange into graphene layers. The thickness of graphene
layers depends on the annealing time and temperature. The formation of few-layer graphene (FLG) typically
requires few minutes annealing of the SiC surface at temperature around 1200 C [44]. More recently, vapor phase
annealing has been used to produce FLG on SiC. At the expense of a higher temperature (typically 400 C above UHV
temperature) [45] this method leads to the formation of FLG on SiC with an improved thickness homogeneity [46].
Another uncertainty involves the different epitaxial growth patterns on different SiC polar face (i.e. Si-face or C-face).
Unusual rotational graphene stacking were observed in multilayers graphene grown on the C-face surface but not on
Si-face surface. Such mismatch of graphene growth process has profound effects on the physical and electronic
properties of epitaxial graphene. On the C-face, the twisted interface leads to the decoupling between different
layers of graphene, each of which behaves as a single layer [47].
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hydrazine as a reductant of graphene oxide although it can be slowly hydrolyzed by water. The NaBH4 treatment
eliminated all the parent oxygen containing groups and the resultant solid became IR inactive like pure graphite.
Other chemical reduction routes including using hydroquinone [59], gaseous hydrogen (after thermal expansion) [60],
and strongly alkaline solutions [61] have also been investigated. Thermal reduction is another approach to reduce GO
to reduced graphene oxide that utilizes the heat treatment to remove the oxide functional groups from graphene oxide
surfaces [62, 63].
Recently, Dubin et al. reported a simple one-step, solvothermal reduction method to produce reduced graphene oxide
dispersion in organic solvent [64]. The solvothermally reduced graphene oxide layers remained in a stable dispersion
after the reaction. This approach provides a simple, low-temperature method to produce reduced graphene oxide.
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Fig. 2. (a) Oxidation of graphite to graphene oxide and reduction to reduced graphene oxide. (b) A proposed reaction
pathway for epoxy reduction by hydrazine.
Fig. 3. Isocyanate treatment of GO where organic isocyanate reacts with the hydroxyl and carboxyl groups of the
grapheme oxide sheets.
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III.
BIOAPPLICATIONS OF GRAPHENE
Probe type
Probe sequence
Target
ssDNA
Graphene
oxide
Graphene
oxide
ssDNA
Graphene
oxide
ssDNA
dsDNA
Graphene
oxide
ssDNA
Graphene
oxide
Hairpinstruct
ured
DNA
Aptamer
5-FAM-AATCAACTG
GGA
GAA
TGTAACTG-3
5-FAM-AGTCAGTGTGGAAAATCTCTAGC3
5-FAMTCTCTCAGTCCGTGGTAGGGCAGGTTGGGG
TGACT-3
5-FAMGGAATTCTAATGTAGTATAGTAATCCGCTC
-3
5(T)15GAGCGGATTACTATACTACATTAGAA
TTCC-3
5-FAM- TCGTTGGAGTTTGTCTG-3
5-Cy5-CCCTAATCCGCCCAC-3
5-ROX-CCTGGTGCCGTAGAT-3
5-DabcylCGACGGAGAAAGGGCTGCCACGTCG-FAM3
5-FAMCTCTCTTCTCTTCATTTTTCAACACAACACA
C-3
5-FAM-GGTTGGTGTGGTTGG-3
Graphene
oxide
Aptamer
SDBSgraphene
Graphene
oxide
Aptamer
Graphene
oxide
ssDNA
MB
and
5-DabcylCGACGGAGAAAGGGCTGCCACGTCG-Cy53
5-H2N-(T)6GCACACGCGCAC-3
383
cDNA
LO(n
M)
N/A
Ref
s
[81]
cDNA
N/A
[83]
Human
thrombin
2.0
[83]
SCV helicase
0.625
[84]
cDNA
0.1
[85]
cDNA
2.0
[86]
[87]
Bovine
thrombin
survivin
mRNA
0.0313
[88]
N/A
[89]
200
[90]
Au
labeled
cDNA
NP-
Mohammad Hakimi and Paransa Alimard, World Applied Programming, Vol (2), No (6), June 2012.
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Mohammad Hakimi and Paransa Alimard, World Applied Programming, Vol (2), No (6), June 2012.
fromenzymatic cleavage during delivery to inter- or intracellular spaces; and (iii) GO-nS can act as a real-time sensing
platform in living cells with high fluorescence quenching efficiency [93].
The capability of graphene for DNA protection from cleavage during cellular delivery has indeed been demonstrated:
MBs can be used as oligonucleotide probes in conjunction with GO-nS to deliver DNA to HeLa cells [89].
III.4.2. GRAPHENE FET FOR LIVING CELL DETECTION
Nanomaterial-based FETs have been proven to be powerful building elements for nanoscale bioelectronic interfaces
with cells and tissues, owing to their ability to form coupled interfaces with cell membranes. Graphene-based FETs
have been reported in recent studies as promising chemical and biological sensors in living cells [94, 95]. For example,
a graphene-based FET has been used to investigate electrogenic cells [94]. The FET conductance signals that are
recorded from beating chicken embryonic cardiomyocytes yields well-defined extracellular signals with a signaltonoise ratio routinely above 4, which exceeds typical values for other planar devices. A graphene-based FET (with
active channel of 20.89.8 m) has also been used as a biosensor to detect hormonal catecholamine molecules in
neuroendocrine PC12 rat adrenal medulla cells [95]. This patterned GO film-based FET has realized the label-free and
real-time monitoring of catecholamine secretion from living cells.
IV.
CONCLUSIONS
It has become evident that the exceptional properties of graphene made it compelling for various engineering and
bioapplications. However, grapheme as a new material still faces many challenges ranging from synthesis and
characterization to the final device fabrication. The synthesis of graphene has made substantial progress, especially
over the past decade. Grapheme has been synthesized by a wide range of chemical synthetic procedures.
As a result of the fascinating properties of graphene, with respect to structures that can be oriented and surfaces that
can be modified, we believe that it offers some important advantages for biotechnological applications, especially in
the areas of bioelectronics, biosensors and medicine.
In this review the various synthesis processes of graphene and selectively biofunctionalization and bioapplication of
grapheme are analyzed.
ACKNOWLEDGEMENTS
Support of this work by the Payame Noor University is gratefully acknowledged.
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Mohammad Hakimi received the B. Sc degree in chemistry from the Ferdowsi University of Mashhad, Iran in
1993 and the Ph. D. degree in inorganic chemistry from Ferdowsi University of Mashhad in 2003. He has
published over 40 peer-reviewed journal papers and contributed to more than 30 conference papers and
presentations. He was collaborated in chemistry group of Newcastel University during 2008. His research
activities include synthesis of Co, Cu, Ag, Au and Cd complexes in low oxidation, metal triazole and triazine
complexes, Amino alcohols complexes, mixed ligand complexes, nano polyoxometales & theirs catalysis and
macrocyclic chemistry. He has published three books in advance inorganic chemistry and inorganic chemistry
issue one and two during 2007 to 2011.
Paransa Alimard was born in 1981 in Iran. She achieved a Masters degree in inorganic chemistry at the Islamic
Azad University of Shahr- e-Rey Branch in 2011. At present, she is in the first year of her Ph.D thesis at Payame
Noor University. Her research interests are in the synthesis of graphene and its chemical applications.
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