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Cariology and Operative Dentistry, Department of Restorative Science, Graduate School, Tokyo Medical and Dental University,
1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan
b
Faculty of Dentistry, School for Dental Technology, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan
c
Department of Conservative Dentistry, Kings College London Dental Institute at Guys, Kings College and St. Thomas Hospitals,
Kings College London, London Bridge, London SE1-9RT, UK
d
Global Center of Excellence (GCOE) Program, International Research Center for Molecular Science in Tooth and Bone Diseases at Tokyo
Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan
article info
abstract
Article history:
Methods: Forty extracted human molars with coronal carious lesions were used in this
9 May 2009
experiment. The occlusal dentin surfaces including the caries-affected dentin in each group
were treated as follows: group 1, rinsed with water; group 2, treated with 6% NaOCl for 15 s;
group 3, treated with 6% NaOCl for 30 s; group 4, application with Accel for 30 s after NaOCl30 s pretreatment. After rinsing with water and air-drying, the treated dentin surfaces were
Keywords:
applied with self-etch systems (Bond Force and Clearfil Protect Bond) according to the
Smear layer
manufacturers instructions, and built-up with resin composite. After 37 8C water storage
Caries-affected dentin
for 24 h, the bonded normal or caries-affected dentin areas were isolated to create an
Dentin bonding
NaOCl
Antioxidant
Results: NaOCl-15 s pretreatment significantly improved the mTBS of both self-etch adhesives to caries-affected dentin, while the 30 s pretreatment did not affect them. For normal
dentin, NaOCl-30 s pretreatment significantly reduced the mTBS of both self-etch adhesives
although the 15 s pretreatment did not alter them. Furthermore, the application of Accel
with a reducing effect increased the mTBS to normal and caries-affected dentin treated with
NaOCl for 30 s.
Conclusions: The effects of NaOCl pretreatment on bonding of both self-etch adhesives were
dependent upon type of dentin (normal and caries-affected dentin) and the treatment time.
# 2009 Elsevier Ltd. All rights reserved.
1.
Introduction
morphological and chemical structures. Caries-infected dentin is infected and quite demineralized, and must be removed.
On the other hand, caries-affected dentin is partially demineralized and should be preserved in clinical treatment
* Corresponding author. Tel.: +81 3 5803 5483; fax: +81 3 5803 0195.
E-mail address: genkainada31990326@yahoo.co.jp (G. Taniguchi).
0300-5712/$ see front matter # 2009 Elsevier Ltd. All rights reserved.
doi:10.1016/j.jdent.2009.06.005
770
2.
771
Table 1 Chemical composition and application mode of the materials used in the study.
Material
Clearfil Protect Bond
(PB; Kuraray Medical)
pH; 2.0 (Primer)
Composition
Procedures
Abbreviations: MDP: 10-methacryloyloxydecamethylene phosphoric acid; HEMA: 2-hydroxyethyl methacrylate; Bis-GMA: Bis-phenol A
diglydidylmethacrylate; MDPB: 12-methacryloy-loxydodecylpyridinum bromide; TEGDMA: triethyleneglycol dimethacrylate.
3.
Statistical analysis
4.
SEM observation of normal and cariesaffected dentin surface after NaOCl pretreatment
6.
Further eight teeth were used for SEM observation. After
treatment with or without NaOCl for 15 and 30 s, the dentin
surface was rinsed off with water and then dehydrated in
ascending concentrations of ethanol in the following steps: 25%
ethanol for 20 min, 50% for 20 min, 75% for 20 min, 95% for
30 min and 100% for 60 min. After the final ethanol step, the
specimens were immersed in hexamethyldisilazane (HMDS) for
10 min, and dried by placement on filter paper inside a covered
glass vial at room temperature for 24 h.19,27 Subsequently, the
dentin surfaces were gold-sputter-coated and observed with a
Scanning Electron Microscope (JXA-840, JEOL, Tokyo, Japan).
5.
Results
The mTBS bond strengths of protect bond (PB) and bond force (BF)
to normal and caries-affected dentin are shown in Table 2.
ANOVA analysis revealed that there was no significant
difference among the materials ( p = 0.32) and there was
significant difference among the pretreatment methods
( p < 0.001) and type of dentin ( p < 0.001) and there was a
SEM observation
Caries-affected dentin
27.9
41.6
33.3
39.9
(6.2) A
(6.6) B
(5.2) A
(6.7) B
029.8
040.6
031.4
035.6
(4.6) C
(7.7) D
(8.5) C
(9.1) CD
772
Inrerfaciala
Mixedb
Cohesive
In dentinc
Protect Bond
Normal
Group 1
Group 2
Group 3
Group 4
Caries-affected
Group 1
Group 2
Group 3
Group 4
Bond force
Normal
Group 1
Group 2
Group 3
Group 4
Caries-affected
Group 1
Group 2
Group 3
Group 4
In resind
0
0
1
0
10
11
11
10
2
1
0
1
0
0
0
1
1
0
0
0
10
11
10
10
1
1
1
2
0
0
1
0
1
0
1
0
9
10
10
11
0
1
0
0
2
1
1
1
1
0
0
0
9
11
10
10
1
0
1
0
1
1
1
2
7.
Discussion
773
774
8.
Conclusions
Acknowledgement
This work was supported by the grant from the Japanese
Ministry of Education, Global Center of Excellence (GCOE)
Program, International Research Center for Molecular
Science in Tooth and Bone Diseases.
references
775
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