CAP is antibiotic that has been used by human for medication and for supplement

food for animal, as its price, high efectivity and good pharmacokinetic characteristic.
Some animal husbandry misapplied it thus some unwanted residue in large amount
left in animal product. Another bad effect of CAP accumulation is causing bone
marrow depression, aplastic anemia, gray baby syndrome and leukemia.
Despite of its bad effect, CAP is still widely used to maximize production of some
aquaculture product such as supplement food for shrimp cultivation and therapy for
precautionary of disease. Therefore larger amount of residues accumulated in
aquaculture product as export commodity. In the last years, exported shrimp from
South east Asian states, has been banned by importer countries as the lock of food
grade standard.
European commission has defined the minimum required performance limit (MRPL)
for CAP in animal food product by the level of 0,3 g/kg (commission
decision/2013/18/ European commission). Trace amounts of CAP in food products
have to be measured to ensure the product wont harm the human health
Therefore, the development of sensitive and rapid method for monitoring CAP
residues in food, such as shrimp. The method includes sample preparation method that
selectively through adsorption process for removing other residues of antibiotics thus
that just obtained matrix components of CAP. Therefore, we need a selective
adsorbent for the CAP. MIP Based adsorbent that can improve the selectivity of the
sample and allows the sample to be analyzed by the simpler and cheaper method of
analysis. MIP is an artificial synthetic material that produces the identifier that can
bind to specific target molecules back in the related compounds.
Analysis CAP has been used with LC/MS/MS, GC, EI-GC/MS, ELISA and HPLC.
HPLC is a potentially profitable method to determine the amount of trace organic
compounds. So in this study will be conducted characterization mip.
Characterization of MIP based on adsorption can be done using a batch system. It is
performed to determine the selectivity of the adsorbent is formed and applied kinetic
models for the mechanisms controlling the adsorption process. Therefore, in this
research, the CAP adsorption method uses MIP-based adsorbent with a batch system.
MIP synthesized using precipitation polymerization method with CAP as a template,
acid methyl methacrylate (MMA) as the functional monomer, ethylene glycol
dimethacrylate (EGDMA) as the crosslinker, chloroform as porogen and benzoyl
peroxide as an initiator. The structure and surface morphology characterized MIP
synthesized using SEM and BET analysis is used to explain the morphological
characterization. Analysis of FT-IR is used to ensure the MIP has been formed, then
the selectivity and kinetics of MIP-based adsorbent will be examined.
Oleh karena itu, diperlukan pengembangan metode yang sensitif dan cepat untuk memantau
residu CAP dalam makanan, seperti pada udang. Metode tersebut mencakup metode

preparasi sampel yang selektif melalui proses adsorpsi untuk menghilangkan residu antibiotik
lain dari komponen matriks sehingga hanya didapatkan CAP. Oleh karena itu, diperlukan
sebuah adsorben yang selektif untuk CAP. Adsorben yang berbasis molecularly imprinted
polymer (MIP) dapat meningkatkan selektivitas sampel dan memudahkan sampel untuk
dianalisis dengan metode analisis yang lebih sederhana dan murah.
MIP merupakan material sintetis buatan yang menghasilkan sisi pengenal yang dapat
mengikat kembali molekul target yang spesifik dalam senyawa yang terkait.
Therefore, the development of sensitive and rapid method for monitoring CAP residues in
food, such as shrimp. The method includes sample preparation method that selectively
through adsorption process for removing residues of antibiotics other than that just obtained
matrix components CAP. Therefore, we need a selective adsorbent for the CAP. Based on
molecularly imprinted adsorbent polymer (MIP) can improve the selectivity of the sample
and allows the sample to be analyzed by the method of analysis is simpler and cheaper.
MIP is an artificial synthetic material that produces the identifier that can bind to specific
target molecules back in the related compounds.

Karakterisasi pada adsorpsi berbasis MIP dapat dilakukan menggunakan sistem batch.
Hal ini dilakukan untuk mengetahui selektivitas adsorben yang terbentuk dan diaplikasikan
model kinetik untuk mengontrol mekanisme pada proses adsorpsi. Oleh karena itu, pada
penelitian ini dilakukan metode adsorpsi CAP menggunakan adsorben berbasis MIP dengan
sistem batch. MIP disintesis menggunakan metode polimerisasi presipitasi dengan CAP
sebagai template, asam metil metakrilat (MMA) sebagai monomer fungsional, etilen glikol
dimetakrilat (EGDMA) sebagai crosslinker, kloroform sebagai porogen dan benzoil
peroksida sebagai inisiator. Struktur dan morfologi permukaan MIP hasil sintesis
dikarakterisasi dengan menggunakan analisis SEM dan BET digunakan untuk menjelaskan
karakterisasi morfologi. Analisis FT-IR digunakan untuk memastikan MIP telah terbentuk,
kemudian diteliti selektivitas dan kinetika dari adsorben berbasis MIP.
Characterization of MIP based on adsorption can be done using a batch system. It is
performed to determine the selectivity of the adsorbent is formed and applied kinetic models
for the mechanisms controlling the adsorption process. Therefore, in this research, the CAP
adsorption method using MIP-based adsorbent with a batch system. MIP synthesized using
precipitation polymerization method with CAP as a template, acid methyl methacrylate
(MMA) as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the
crosslinker, chloroform as porogen and benzoyl peroxide as an initiator. The structure and
surface morphology characterized MIP synthesized using SEM and BET analysis is used to
explain the morphological characterization. Analysis of FT-IR is used to ensure the MIP has
been formed, then examined the selectivity and kinetics of MIP-based adsorbent.
Berdasarkan uraian latar belakang di atas, maka dapat dirumuskan masalah sebagai
berikut.
Based on the description above background, it does appear the following issue.

1. Apakah MIP yang disintesis dengan teknik presipitasi menggunakan CAP sebagai
template, MMA sebagai monomer, EGDMA sebagai crosslinker, kloroform sebagai
porogen dan benzoil peroksida sebagai inisiator dapat digunakan sebagai adsorben
yang selektif untuk CAP pada sistem batch?
1. Is MIP synthesized by precipitation technique using CAP as a template, MMA as
monomer, EGDMA as the crosslinker, chloroform as porogen and benzoyl peroxide as an
initiator can be used as a selective adsorbent for CAP in a batch system?
1. Bagaimana kinetika adsorpsi dari MIP terhadap analit CAP pada sistem batch?
2. How is the kinetic adsorption of the analyte CAP MIP on a batch system?
3.
Bagaimana adsorpsi isotermal dari permukaan MIP terhadap analit CAP di
dalam sistem batch?
3. How is the isothermal adsorption of the analyte to the MIP surface of the CAP in
the batch system?
2. Berapakah besarnya kapasitas adsorpsi dan faktor pemekatan CAP pada MIP dalam
sistem batch ?
3. What is the magnitude of adsorption capacity and concentration factors of CAP on
the MIP in a batch system?
4. Tujuan umum
5. Mengetahui selektivitas, faktor pemekatan, kapasitas dan kinetika adsorpsi CAP pada
adsorben MIP yang disintesis dengan teknik presipitasi dalam sistem batch.
The general objective
Knowing selectivity, concentration factor, adsorption capacity and kinetics of CAP on
MIP adsorbents were synthesized by precipitation techniques in a batch system
Tujuan khusus
1. Mengetahui kemampuan MIP yang disintesis menggunakan teknik polimerisasi
presipitasi dengan CAP sebagai template, MMA sebagai monomer, EGDMA sebagai
crosslinker, kloroform sebagai porogen dan benzoil peroksida sebagai inisiator
sebagai adsorben yang selektif untuk CAP pada sistem batch.
2. Mengetahui kinetika adsorpsi dari MIP terhadap analit CAP di dalam sistem batch.
3. Mengetahui mekanisme interaksi adsorpsi dari analit CAP dengan permukaan MIP
dalam sistem batch.
4. Menentukan kapasitas adsorpsi dan faktor pemekatan CAP pada adsorben MIP dalam
sistem batch.

special purpose
1. Determine the ability of MIP synthesized using precipitation polymerization
technique with CAP as a template, MMA as monomer, EGDMA as the crosslinker,
chloroform as porogen and benzoyl peroxide as an initiator as selective adsorbents
for CAP in a batch system.
2. Determine the kinetics of adsorption of the analyte MIP CAP in the batch system.
3. Determine the mechanism of adsorption of analyte CAP interaction with the
surface of MIP in a batch system.
4. Determine the adsorption capacity and concentration factor at the CAP MIP
adsorbent in a batch system.
Manfaat Penelitian
Manfaat dari penelitian ini adalah diharapkan dapat diperoleh informasi tentang
karakteristik dan kinetika adsorpsi CAP pada adsorben MIP yang disintesis dengan teknik
presipitasi dan dihasilkan MIP sebagai adsorben yang selektif untuk pemisahan dan
pemekatan CAP pada proses analisis CAP.
benefits of Research
The benefits of this research are expected to obtain information about the
characteristics and adsorption kinetics of CAP on MIP adsorbents were synthesized
by precipitation techniques and produced MIP as a selective adsorbent for the
separation and concentration of CAP in the CAP analysis.