CHM 3120 Exam 3

(Form Code A)

November 20, 2014

Instructions: On your scantron sheet enter your name, UF ID number (start on the first space and leave
the last space blank), and Form Code (see above). This exam consists of 20 multiple choice questions each
worth 10 points for a total maximum of 200 pts. You may retain your exam sheet. Turn in only the
scantron. Bubbling errors of any kind will count as an incorrect response.
1. Which of the following is not true concerning dispersive (gratings) vs. non-dispersive Photodiode
Array (PDA) optical detection systems: (A) PDA analysis is faster. (B) PDAs show poorer
S/N. (C) PDAs show better resolution. (D) PDAs are more rugged. (E) PDAs experience more stray
light. (Both B and C are acceptable)
2. Which of the following statements concerning electromagnet radiation is not true?
(A) Energy is directly proportional to frequency. (B) Energy is indirectly proportional to wavelength.
(C) Red light is more energetic than blue light. (D) Microwaves are lower energy than IR radiation.
(E) UV is more energetic than Visible radiation
3. Which of the following is not a correct statement concerning mass spectrometry (MS)?
(A) The combination of a magnetic sector and ESA focusing allows high MS resolution (B) All MS
ions of the same mass have the same velocity. (C) In calculating resolving power for MS, m/m gives
half the value of m/m1/2 . (D) Chemical ionization produces less fragmentation than does electron
ionization.
4. What accelerating voltage is required to pass a triply charged nitric oxide (NO) molecule through the
exit slit of a magnetic sector mass spectrometer if the magnet has a field strength of 0.240T and the
radius of curvature through the magnetic field is 12.7 cm? [e = 1.6 x 10-19 C]
(A) 2.495 x 103 V (B) 4.469 x 103 V (C) 3.607 x 103 V (D) 2.979 x 103 V (E) other
5. In time-of-flight (TOF) mass spectrometry , which of the following is not true:
(A) A reflectron TOF reduces the amount of ion energy spread. (B) In a linear TOF, a shorter flight
tube gives decreased resolution. (C) In a reflectron TOF, the lower energy ions of a given m/z traverse
a longer flight path. (D) Ions must be injected as pulses (not continuously) for proper operation.
6.

A 2.96 104 M solution of compound A exhibited an absorbance of 0.624 at 238 nm in a 1.000-cm

cuvet; a blank solution containing only solvent had an absorbance of 0.029 at the same wavelength.
An unknown solution of compound A in the same solvent was diluted from an initial volume of
5.00 mL to a final volume of 25.00 mL and then showed an absorbance of 0.533. What is the molar
concentration of A in the unknown solution?
(A) 3.77 x 10-3 (B) 4.69 x 10-4 (C) 4.90 x 10-4 (D) 1.25 x 10-3 (E) other

7. What is the emission wavelength (nm) of excited atoms that lie 3.787 x102 kJ per mole above the
ground state?
(A) 316.1 (B) 361.1 (C) 275.0 (D) 589 (E) other
8. Element X with emission at 550 nm [degeneracies (g*/go) = 3] is excited
in a flame with a temperature of 3000K. What is the calculated N*/No ratio?
(A) 2.03 x 10-4 (B) 3.27 x 10-4 (C) 1.78 x 10-4 (D 4.90 x 10-4 (E) other
9. A 4.236 g quinine tablet was dissolved in 0.150 M HCl and diluted to 500 mL of solution. Dilution
of a 25.0 mL aliquot to 100 mL yielded a solution that gave a fluorescence reading at 347.5 nm of 438
arbitrary units. A second 25.0 mL aliquot of the original solution was mixed with 4.0 mL of 50
ppm quinine solution before dilution to 100 mL. The fluorescence intensity of this solution was 642.
Calculate the percent quinine in the tablet.
(A) 0.32 % (B) 0.41% (C) 0.25% (D) 0.20% (E) other
10. Fe+3 reacts with [SCN]-1 to form a colored complex, Fe(SCN)n +3-n
that absorbs at 455 nm. A plot of absorbance vs. mole fraction of [SCN]-1
yields a maximum at 0.67. What is the value of n in Fe(SCN)n+3-n?
(A)one (B) two (C) three (D) four (E) other

11. Which of the following concerning flow injection is not correct?

(A) The advantages of flow injection include speed, automation, and low cost.
(B) The concentration profile of product passing through the detector depends on sample volume,
flow rate, reaction rate, and temperature.
(C) Sequential analysis suffers from larger reagent volumes and more waste.
(D) Reactions in the flowing system normally do not reach chemical equilibrium.
(E) Multiple reagent injections provide greater flexibility for analyte reactions.
12. Which of the following statements about fluorescence is correct?
(A) Fluorescent lamps employ a Hg vapor discharge, which emits in the visible region.
(B) An excitation spectrum is obtained by holding the excitation wavelength fixed.
(C) An excitation spectrum looks much like an absorption spectrum.
(D) The lifetime of fluorescence is generally longer than phosphorescence.
(E) Luminescence is inherently less sensitive than absorption.
13. At 510 nm a solution of KMnO4 containing 20.0 ppm manganese has an absorbance of 0.700 for a
1.00 cm optical path. What is the molar absorptivity of KMnO4 at 510 nm?
(A) 5.51 x 103 (B) 1.92 x 103 (C) 3.23 x 103 (D) 3.98 x 103 (E) other
14. A sample containing 32.6 g Mo/mL gave an absorbance of 0.025. If sensitivity is defined as the
concentration of Mo giving 99% transmittance, what is the sensitivity (g/mL) for Mo?
(A) 4.76 (B) 3.03 (C) 10.97 (D) 5.69 (E) other
15.

16.

Which of the following statements is incorrect? (Both C and E are acceptable)

(A) A double beam spectrophotometer allows compensation for changes in detector sensitivity
as a function of wavelength.
(B) Resolution of a photomultiplier-based instrument depends on the exit slit width and
monochromator dispersion.
(C) Photoconductive detectors (IR) operated at low temperatures show a reduced S/N.
(D) Source intensity in the UV-VIS region varies significantly with wavelength.
(E) Narrowing a spectrophotometer entrance slit width decreases resolution at the detector.
.
Which of the following statements is not correct relative to Beers Law?
(A) Beers Law applies to both atomic and molecular absorption. (B) A monochromatic light
source is required for both atomic and molecular absorption. (C) At low concentrations, selfabsorption is not a problem. (D) Increasing cell path length enhances analyte detection limits. (E)
Both absorbance and molar absorptivity vary with wavelength.

17.

Which of the following statements concerning atomic spectroscopy is not correct?

(A) Atomic line widths increase with increasing pressure.
(B) The Doppler effect reduces atomic line widths.
(C) Gas phase collisions shorten excited state lifetimes.
(D) The hollow cathode lamp produces line spectra.
(E) The graphite furnace is not particularly useful for atomic emission.

18.

A signal is averaged to obtain a signal-to-noise ratio of 30/1. If the non-averaged spectrum

showed a signal-to-noise of 5/1, how many spectra must have been averaged to obtain such an
improvement?
(A) 9 (B) 36 (C) 25 (D) 16

19.

20.

(E) other

Which of the following statements about immunoassays is not true?

(A) Fluorescence is an important detection mode. (B) An antibody is an antigen produced by the
immune system. (C) Time resolved fluorescence often can enhance immunoassay sensitivity. (D)
Antibody 1 is specific for the antigen. (E) Antibody 2 is specific for the antigen.
What is the flight time in microseconds in a TOF mass analyzer for a +2 charged ion of 435.67
mass accelerated into a 1.850 meter flight tube with an acceleration voltage of 18.0 kV?
(A) 22.7 (B) 4.54 (C) 14.6 (D) 8.82 (F) other