Professional Documents
Culture Documents
of the
University
of Chemical
Technology
Metallurgy,
1, 2009
Journal
of the
University
of Chemical
Technology
andand
Metallurgy,
44,44,
1, 2009,
50-54
ABSTRACT
In the present work the rheological behaviour of the whole blood of healthy persons in relation to its parameters
(hematocrite, hemoglobin and RBC) was investigated. It was found that the whole blood exhibits non-Newtonian behaviour
which can be described by the power law rheological model. The blood apparent viscosity in women is lower than that in
men which can be explained by lower HCT values for the females. The observed HCT values are not always directly
related to the erythrocytes number it depends also on the RBC dimensions. The dependencies of the rheological parameters K and n on the hematocrite values are found. Both dependencies could be described by linear relationship with HCT.
The dependencies of the rheological parameters on the RBC concentration are also found. The dependency of K on RBC
could be described by 2nd degree polynomial relationship. On the other hand the flow index values are almost unaffected
by the erythrocytes concentration. So it could be taken a mean value for n.
Keywords: blood rheology, hematocrite, RBC concentration.
INTRODUCTION
Biorheology is the branch of biological sciences
that studies the flow and deformation of biological material under the influence of constraints applied to it.
The part of the biorheology focusing on blood is termed
hemorheology. Its purpose is therefore to study the flow
of the blood in interaction with its surrounding environment, in both macro- and microcirculation. The
blood is a fluid circulating in the human body and transferring the oxygen from the lungs to all important organs of the body. As a tissue the blood consists of cells
and intracellular substance plasma, i.e. the blood can
be treated as a suspension. The blood cells are: erythrocytes or red blood cells (Er or RBC), leucocytes (leuc)
and trombocytes (Trh) suspended in the blood plasma.
One of the most important characteristics of the
blood is the hematocrite which is the relationship be-
50
Plaques or trombocytes
Leucocytes and erythrocytes have their origin in
cells maturation. In the contrary, plackets form through
the fragmentation process. Bloods plaques amount is
150000 to 300000 per mm3. They are in charge of avoiding bloods extravasations if an eventual damage takes
place. Plaques get together over a damage forming a
plaque cap. They also play an important role in protein
activation during coagulation since they free
tromboplastine and on the other side serotonin (vessel
constrictor) acting over straight muscle cells of the vessels, thus helping to diminish bloods flow in the injured area.
Plasma: an intracellular substance
Plasma is a colloidal solution, or a system of
proteins dissolved in an aqueous medium. These proteins are called plasmatic proteins and are mostly synthesized by the liver. The most abundant and the largest
is the albumin. Its main function is to transport different molecules. It possesses different electrical charges
on its surface which allows establishing unions between
the different elements it carries. Thus, molecules with
low solubility can be carried. The albumin also, joins
water molecules which produce an aqueous retention
within the intravascular compartment. Water is the largest component of plasma and acts as a solvent of plasmatic proteins and inorganic salts. These three components regulate arterial pressure.
It is evident that the hydrodynamic and mass
transfer functions of the blood are closely related to its
composition and the amount of the suspended in it red
blood cells. It could be estimated by measurement of
the rheological properties of the blood.
Recently the number of publications on this question
increases [2-7]. They are dedicated to the relationship between the blood viscosity and its other parameters (hematocrite,
lactates, etc.) in trained and untrained persons as well as to
the effect of the exercise on these properties.
In the literature different experimental conditions and methods are reported. Different rheometers
were used for the studies (capillary, rotational, oscillating, etc.) the blood samples were prepared by different
methods, and their properties were followed for temperatures of 37 0C or of 20 0C. Therefore the data are to
be treated carefully to avoid possible errors.
51
100
, mPa
Blood rheology
Rheological measurements of whole blood and
red blood cell suspensions demonstrate unique nonNewtonian behaviour, i.e. yield stress, shear thinning,
thixotropy and viscoelasticity. However, few studies have
quantitatively considered the viscoelastic relaxation of
blood cells and links with bulk rheology are not established [8].
A number of the literature data concern the blood
rheology studied with rotational viscometers with coaxial cylinders or cone-and-plate [2, 9,12], falling ball
viscometers at high shear rates [10], tube viscometers
[11] at low shear rates, and oscillating flow tube viscometers to measure the elastic properties of blood [12].
Different shear rates and temperatures, as well as different methods for blood preservation are used, so it is
quite difficult to make a direct comparison of the results of different authors. Usually, only an apparent viscosity for a given shear rate (or shear stress, depending
on the measuring device) are presented, not the whole
flow curves.
The aim of the present work was to study the
rheological behaviour of the whole blood of healthy
persons in relation to its parameters (hematocrite, hemoglobin and RBC).
80
TAU2j
60
TAU3j
40
TAU4m
20
TAU5m
0
0
500
1000
,s
1500
-1
EXPERIMENTAL
Materials and methods
Blood was sampled from 10 healthy male and
female volunteers in vacuteiners containing EDTA as
anticoagulant. The rheological measurements were made
using a co-axial cylinder viscometer Rheotest RV2.1
(Germany) with N cylinder (sample volume of 11 cm3).
The flow curves were taken at temperature of 200C.
Unique analysis with Z cylinder showed no slip effect
on the wall, but it was found that sensibility of the device was not enough for that experiments. That was the
reason to continue the experiments with N cylinder.
The hematological analyses were done in authorized
laboratory, where the values of hematocrite, RBC and
hemoglobin were determined.
RESULTS AND DISCUSSION
In Fig. 1 four typical flow curves: two for male
and two for female persons, are presented. Samples 2
52
= K n
Here K is the consistency index, characterizing
n
the sample consistency, mPas ;
n is the rheological flow index (more it differs
unity, more the fluid is non-Newtonian);
is the shear stress, mPa;
is the shear rate, s-1.
The rheological parameters of the samples were
found statistically using MSExcell.
In Fig. 2 the dependencies of the rheological parameters on the hematocrite values are presented. It was
found that both dependencies could be described by
linear relationship with HCT. The equations found give
satisfactory accuracy: Maximal relative error for K being 14 %, for n 4.9 %
1,4
1,2
K,n
1
0,8
Linear (n)
0,6
Linear (K)
0,4
0,2
K,n
1,4
1,2
1
0,8
0,6
0,4
0,2
0
K
n
Linear (n)
Poly. (K)
0
0,3
0,4
RBC,T/L
0,5
HCT
K = 0.8693HCT + 0.6707
n = 0.4604 HCT + 0.3994
In Fig. 3 the dependencies of the rheological parameters on the RBC concentration are presented. It
was found that the dependency of K on RBC could be
described by 2nd degree polynomial relationship:
CONCLUSIONS
From the experiments done the following conclusions could be driven:
The whole blood exhibits non-Newtonian
behaviour which can be described by the power law
rheological model.
The blood apparent viscosity in women is lower
than that in men which can be explained by lower HCT
values for the females.
The observed HCT values are not always directly related to the erythrocytes number it depends also
on the RBC dimensions.
The dependencies of the rheological parameters K and n on the hematocrite values are found. Both
dependencies could be described by linear relationship
with HCT.
The dependencies of the rheological parameters on the RBC concentration are also found. The
dependency of K on RBC could be described by 2nd
degree polynomial relationship. On the other hand the
flow index values are almost unaffected by the erythrocytes concentration. So it could be taken a mean
value for n.
Acknowledgements
This work was supported financially by the Research Sector of UCTM-Sofia, contract No 2/87-2009.
53
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