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(a)
(i)
(ii)
(b)
(c)
(i)
max 2
max 3
(ii)
(iii)
(i)
(ii)
At low temperatures/9C;
Relatively little kinetic energy/molecules only moving slowly;
Fewer collisions with enzyme;
Slower rate of reaction/takes longer for lactose to be reduced/some
substrate goes through unchanged;
or
Enzyme concentration limiting;
Substrate in excess;
Saturation of active sites/all occupied;
Some substrate goes through unchanged;
(d)
(i)
(ii)
max 3
1
[16]
2.
(a)
(i)
Energy put in to get reaction started (Look for idea of getting started);
(ii)
Sciencemathsmaster.weebly.com
(b)
(c)
(d)
(i)
(ii)
(i)
(ii)
max 2
3
[12]
3.
(a)
(i)
(ii)
Double;
Increase of 10C doubles rate of reaction;
OR
Increase;
Increased KE/ increased energy of molecules / increased movement;
(b)
To show that enzyme was responsible for conversion, (no other factor);
2
1
[5]
4.
(a)
(b)
(c)
(i)
Transfers phosphate;
(ii)
(i)
(ii)
C12H22O11;
Sciencemathsmaster.weebly.com
(d)
3
[10]
5.
(a)
(i)
(ii)
max. 3
(b)
(c)
1
[6]
6.
(a)
(i)
(ii)
(b)
max 3
max 2
[8]
7.
(a)
(i)
(ii)
Sciencemathsmaster.weebly.com
(b)
(c)
Keeps pH constant;
So proteins / enzymes in mitochondria not denatured / affected;
(d)
(e)
6 max
[15]
8.
(a)
(b)
(c)
(i)
Plasma cells;
(ii)
(d)
2 max
3 max
Measles
One antigen/
unchanging
One type of memory
cell/ antibody
needed;
Influenza
Several antigens/
changing
Several types of
memory cell/
antibodies needed;
[8]
9.
(a)
(i)
Sciencemathsmaster.weebly.com
(ii)
(iii)
(b)
2 max
(i)
5;
(ii)
3
[8]
10.
(a)
(i)
(ii)
(iii)
(b)
2 max
[7]
11.
(a)
(b)
max 2
[5]
12.
(a)
Sciencemathsmaster.weebly.com
(b)
It is a protein;
[Reject: blue or pink colour]
(i)
(ii)
(iii)
(c)
max 2
[9]
13.
(a)
(i)
(ii)
(b)
(c)
(i)
(ii)
Sciencemathsmaster.weebly.com
max 3
(d)
max 6
[15]
14.
(a)
(i)
Hydrolysis;
(ii)
(iii)
(b)
max 2
(c)
(i)
(ii)
(d)
1
2
3
4
5
6
7
max 6
[15]
15.
(a)
(i)
Sciencemathsmaster.weebly.com
(ii)
max 3
(b)
(Control) to show that sand did not affect reaction (with ground liver);
(c)
(i)
(ii)
2
[9]
16.
(a)
(b)
(i)
(ii)
(i)
To keep pH constant;
A change in pH will slow the rate of the reaction / denature the amylase /
optimum for reaction;
(ii)
Purple/lilac/mauve/violet;
Do not allow blue or pink.
(iii)
Protein present;
The enzyme/amylase is a protein;
Not used up in the reaction / still present at the end of the reaction;
max 2
[7]
17.
(a)
(b)
Sciencemathsmaster.weebly.com
(c)
3 max
[7]
18.
(a)
Lilac/purple/mauve/violet;
Xanthine oxidase is a protein;
(b)
3
[7]
19.
(a)
(b)
(i)
150;
(ii)
27;
100;
number of peptide bond hydrolysed = total number present / all peptide
bonds have been hydrolysed;
(c)
(d)
(e)
Sciencemathsmaster.weebly.com
3 max
(f)
20.
(a)
(b)
(c)
(d)
3
[8]
21.
(a)
amino acid;
(b)
violet/purple/mauve/lilac;
(c)
3
[5]
Sciencemathsmaster.weebly.com
10
22.
(a)
(b)
max. 4
enzymes involved;
formation of the enzyme-substrate complex reliant on the correct pH;
pH affects the active site;
by disrupting bonds/altering charge;
lowering temperature will reduce pH;
enzymes have optimum pH;
pH change will slow the rates of reactions;
max. 5
[9]
23.
(a)
(b)
(c)
(i)
(ii)
No, would show that there was nothing else in the potato that
produced the reaction;
1
[6]
24.
(a)
(b)
(c)
COOH;
NH2.
(Chain folded into) tertiary structure / particular 3-D shape (not just globular);
active site formed;
substrate molecules fit this site:
reduce activation energy for reaction
2
[7]
Sciencemathsmaster.weebly.com
11
25.
(a)
(b)
(i)
maltose.
(ii)
max 4
[8]
26.
(a)
(b)
(i)
(ii)
max 3
[6]
27.
(a)
Active site;
(b)
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12
(c)
(d)
(i)
(ii)
Max rate =
Dis tan ce 11 11
/ /
;
Time
4 4 60
28.
(a)
(b)
Temperature
Rate of reaction increases;
Increasing temperature increases rate of movement of molecules/
kinetic energy;
Collide more often/substrate enters active site more often/more
enzyme-substrate complexes formed;
Up to optimum;
Rate of reaction decreases;
High temperatures cause denaturation/loss of tertiary structure/3D structure;
By breaking specified bonds (not peptide bond);
Active site altered/substrate cannot bind/fit/
(i)
(ii)
Competitive inhibition;
Ethanol/ethylene glycol compete for same active site;
Molecules similar shape (not same)/both complementary to/both
fit active site;
Prevents/slows production or build up of oxalic acid/toxic products;
Ethylene glycol excreted (without causing death);
4
[14]
Sciencemathsmaster.weebly.com
13
29.
(a)
(b)
(i)
48
(ii)
description
increase up to 48 / optimum allow ECF from (i);
decrease above 48 / optimum allow ECF from (i);
explanation of increase
increased KE / move faster;
therefore more collisions / more enzyme-substrate complexes formed;
with active site;
(ii)
(c)
56-58
explanation of decrease
denaturation / 3D structure changed / tertiary structure changed;
detail e.g. breaking of hydrogen / sulphur bonds; (reject peptide bonds)
shape of active site changed;
substrate no longer fits;
6 max
[14]
30.
(a)
(b)
Sciencemathsmaster.weebly.com
14
(ii)
31.
(a)
(i)
(ii)
(b)
3 max
2 max
[7]
32.
(a)
C12 ; H22O11 ;
(b)
(i)
(ii)
2
[6]
33.
(a)
Sciencemathsmaster.weebly.com
15
(b)
3
[6]
34.
(a)
(b)
(c)
(d)
7.6 5.6
x 100;
7.6
= 26.32%; (accept 26% or 26.3%)
(correct answer = 2 marks)
decrease in rate
(principle x 100 = 1 mark)
maxrate
2 max
1
[7]
35.
(i)
absorbs/transports triglycerides/fats/lipids/chylomicrons;
(ii)
2
[3]
36.
(a)
(i)
absorbed by diffusion;
no energy/ATP available / active transport requires energy/ATP;
2 max
Sciencemathsmaster.weebly.com
16
(b)
(c)
2
[7]
37.
(a)
(b)
(i)
(ii)
curve drawn above curve on graph and starting at same point but
finishing above;
(i)
1
2
3
(ii)
4
5
6
7
8
9
7 max
Sciencemathsmaster.weebly.com
17
38.
(a)
(b)
(c)
2 max
(d)
2
[7]
39.
(a)
(i)
Glucose;
Fructose;
Any order.
(ii)
(i)
(ii)
Sciencemathsmaster.weebly.com
18
(iii)
1
[9]
40.
(a)
3 max
(b)
2 max
(i)
(ii)
2 max
[8]
Sciencemathsmaster.weebly.com
19