JATROPHA (Jatropha curcas) SEED GERMINATION

EFFECT FROM SEED SOAKING AND TIME

TEMPERATURE IN DIFFERENT COMBINATION

By :

Anggi Nindita

SURFACTANT AND BIOENERGY RESEARCH CENTER

Bogor Agricultural University
Kampus IPB Baranangsiang
Jl. Raya Pajajaran, Bogor – Indonesia
E-mail : info@sbrc-ipb.com
sbrc.info@gmail.com
Telp : 0251- 30970; Fax : 0251 - 330977
 OUTLINE
I. INTRODUCTION
II. MATERIAL AND METHODS
III. RESEARCH
I. INTRODUCTION
 Jatropha Seed
 Availability good quality seed is needed to
Jatropha
 Availability of High Seed Production is
needed
 Soaking Temperature and Time of Soaking is
one of factor to Seedling growth requirement
JATROPA PLANT
(Jatropha curcas L)
 Family of Euphorbiaceae
 Bush or small plant with irregular
branches, 1-7 m height,
 Grow wild or grown as fence,
 Grow well in infertile land with dry
weather,
 Widespread in almost all regions
in Indonesia, Live until 25-30
years,
JATROPA SEED

 Round-oval shaped seed,

 Blackish-brown seed coat,
 Brownish-white seed,
 Size : length 2 cm, width 1cm,
Advantages of Jatropa Curcas Linn as Biodiesel Raw Material

• seed has high oil content (30 - 50 %)

• Oil is not edible --> not interfere edible oil supply

• Can be grown and developed in dry and marginal lands (10

million ha of potential land in Indonesia)

• Easy to grow and widely adaptable

• Does not drain soil nutrients nor reduce water reserve,
makes soil fertile, instead
Growth Requirements

 Grow well in lowland up to 1000 m above sea

level,

 Grow at rainfall of 300-2380 mm/yr, Optimum

rainfall: 625 mm/yr,

 Grow well in porous as well as infertile

(marginal) soil,

 Required average temperature is 20-28 oC,

 Requirement acidity of soil between 5 – 6,5

 Can’t grow in peat moss land

CONVENTIOANAL SEEDLING METHOD
TISSUE CULTURE SEEDLING METHOD
OBJECTIVE
 High Production of Seedling growth
 High Production Of Jatropha Plantation
 Determined Right Temperature and Time
to seed soaking treatment
II. RESEARCH METHODS
II. MATERIAL AND METHODS
Analysis Using Statistical Analysis System (SAS) and Duncan
Multiple Range Test (DMRT) Interval level 5%

EXPERIMENT DESIGN

Yijk = + Ti + Jj + (TJ)ij + Pk

Completely Randomized Block Design

This method of this research used Random Group
Design (RGD), arranged in two factorial.
The factor is as follow :

1. Temperature of Seed Soaking , Consist of three:

Tb = Room Temperature, 270C
T40 = 40 oC Temperature
T70 = 70 oC Temperature
2. Time of seed soaking, consist of two :
J1 = Time of Seed Soaking 6 Hour
J2 = Time of Seed Soaking 12 Hour
Treatment

 This experiment was conducted at

Surfactant and Bioenergy Research
Center
 Seed Soaking Temperature are
Temperature 27 oC (Tb), 40 oC (T40) and
Temperature 70 oC (T70)
 Time Of Seed Soaking Temperature are
6 Hour and 12 Hour
 Experiment Flow chart

Soaking Seed in Different

Temperature

Planting

Time of Soaking
Seed
Preparation

Planting

Without Soaking
SEED SOAKING AND TIME TEMPERATURE
TREATMENT
Normal Germinates Criteria

Normal
III. RESEARCH RESULTS
 Recapitulation of Anova and Statistic Analysis
No Parameter Treatment
Soaking Time of Soaking Interaction
DegreeTemperature Treatment
1 Germination Ability ** ** *
2 First Count Day of Germination nd nd nd
3 Dry Weight of Seed Germination ** * nd
4 Percentage of Normal Seed Germination Per
Day
Day 1 nd nd nd
Day 2 nd nd nd
Day 3 nd nd nd
Day 4 ** * **
Day 5 nd nd nd
Day 6 nd nd nd
Day 7 nd nd nd
Day 8 ** ** **
Day 9 ** ** **
Day 10 ** ** **
Day 11 ** ** **
Day 12 ** ** **
Day 13 ** ** **
Day 14 ** ** **
Percentage of Normal Germination
25.00
Number of Seed Germination

20.00
T0J0
TbJ6
15.00
TbJ12
T40J6
10.00 T40J12
T70J6
T70J12
5.00

0.00
1 2 3 4 5 6 7 8 9 10 11 12 13 14
Day After Plant
First Count Of Germination
First Count of Germination

0.09
0.08
Number of Seed

0.07
Germination

0.06
0.05
0.04
0.03
0.02
0.01
0.00
T0J0 TbJ6 TbJ12 T40J6 T40J12 T70J6 T70J12
Treatment Combination
 Interaction of Time Seed Soaking Temperature and Time Seed Soaking
Temperature to Percentage Of Normal Germination From SAS Analysis
and DMRT.

Treatment Day After Planting

Combination 8 9 10 11 12 13 14
TbJ6 8,33a 12,67ab 14,33a 17,33ab 17,33ab 18,67a 18,6
7a
TbJ12 7,33ab 8,67b 10.00a 13,33b 13,33b 13,67b 13,6
7b
T40J6 10,33a 15,33a 15,33a 18,33a 18,33a 20,67a 19,0a
T40J12 0,67c 1,67c 2,33b 1,67c 1,67c 1,67c 1,67c
T70J6 0c 0c 0b 0c 0c 0c 0c
T70J12 0c 0c 0b 0c 0c 0c 0c

Note : Based on Duncan Multiple Range Test interval 5%

Germination Ability
Germination Ability

0.80
0.70
0.60
Number of Seed
Germination

0.50
0.40
0.30
0.20
0.10
0.00
T0J0 TbJ6 TbJ12 T40J6 T40J12 T70J6 T70J12
Treatment Combination
Interaction of Time Seed Soaking Temperature and Time Seed Soaking
Temperature to Germination Ability From Analysis of SAS and DMRT.

Treatment Combination Time of Seed Soaking

6 Hour (J6) 12 Hour (J12)

Temperature 270C (Tb) 0,75a 0,54b

Temperature 400C (T40) 0,76a 0,07c

Temperature 700C (T70) 0c 0c

Note : Based on Duncan Multiple Range Test interval 5%

Germination Dry Weight
Germination Dry Weight

70.00
Number of Seed Germination

60.00

50.00

40.00

30.00

20.00

10.00

0.00
T0J0 TbJ6 TbJ12 T40J6 T40J12 T70J6 T70J12
Treatment Combination
 Effect of Seed Soaking Temperature Dry Weight of Seed
Germination From SAS Analysis and DMRT.

Treatment Dry Weight of Seed

Germination (g)

Temperature 270C (Tb) 50,23a

Temperature 400C (T40) 11,32b

Temperature 700C (T70) 0b

Note : Based on Duncan Multiple Range Test interval 5%

 Effect of Time of Seed Soaking Dry Weight of Seed
Germination From SAS Analysis and DMRT.

Treatment Dry Weight of Seed

Germination (g)

Time Temperature 28,124a

6 Hour (J6)

Time Temperature 12,92b

12 Hour (J12)

Note : Based on Duncan Multiple Range Test interval 5%

CONCLUSION
Different Germination Growth To
Jatropha

T0J0 T40J6 T40J12

No Germinates are
growth at 70 C
degreee

TbJ6 TbJ12
Documentation
 THANK YOU
Surfactant and Bioenergy Research Center SBRC
Bogor Agricultural University
Telp : (0251) 330 970, Fax : (0251) 330 977
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