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Culture Documents
What is Microbiology?
Microbiology the study of organisms that are difficult
or impossible to observe unaided.
Bacteriology
(Bacteria)
Virology
(Viruses)
Mycology
(Fungi)
Medical Microbiology
Disease causing microbes.
Environmental Microbiology
Studies microorganisms outside of medical importance.
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Germ Theory
Microorganisms are the causative agents for
many diseases and the spread of disease goes
hand in hand with their transmission.
Kochs Four Postulates:
Step 1:
Association
Step 2:
Isolation
Step 3:
Inoculation
Step 4:
ReIsolation
Important Terms
Nosocomial An infection not present upon admission
to a hospital but incurred while being treated there.
Aseptic Free of pathogenic organisms.
Pathogen Disease causing agent.
Inoculation Implantation of micro-organism into or
upon culture media.
Turbid Cloudy appearance of a nutrient solution in a
test tube due to growth of microbe population.
Safety in Lab
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Disposal of Materials
Sterilization /decontamination for most
materials prior to use or being disposed off.
Sterilization techniques commonly used:
Autoclave
Incineration
UV cross-link
Containment
Containment of Micro-organisms:
Primary Containment Protection of
the Lab personnel and the lab
environment.
Secondary Containment Protection
of the outside Lab environment from
exposure to infectious organisms.
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An introduction to Microscopy
Antonie Philips van Leeuwenhoek:
(October 24, 1632 August 26, 1723)
commonly referred to as "the Father
of Microbiology", and considered to
be the first microbiologist.
Bright field or light microscope
because objects examined appear as
dark objects in bright visual field.
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Parts of a Microscope
0.61
Resolution : =
Oil Immersion
Highest
magnification of a
light microscope.
Oil has same
refractive index of
glass and prevents
light loss.
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Microscope Care
Transport Always use both hands. One hand to grasp the
microscope around the arm and the second to support it at the
bottom.
Electric cord Never leave the cord dangling, wrap it loosely
around the base of the scope.
Protection against dust and chemicals If a dust cover is provided,
be sure to use it.
Lens cleaning unacceptable images mostly result from dirty
lenses. Cleaning dirt , dust and stain off the lens is must after each
use. To avoid scratches , only LENS PAPER should be used. Small
amount of alcohol or xylene can be used to remove oily residue.
Bacillus
Spirillum
Cocci
Microscope use.
Staining of microorganisms and use of
microscopes.
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Isolation of Microbes
This exercise will be for a lab report
Ubiquity of Microbes:
Microbes inhabit almost every environment (ubiquitous).
Although microbes inhabit many locations, their detection requires that they
be:
removed
transferred to growth media
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Staining Microbes
Most microorganisms are transparent on slides
and nearly impossible to differentiate.
Stains are used for visualization and come in
many varieties:
Simple
Differential
Negative
Positive
Negative Stain
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