SY-8100 Manual Operacion

Users Handbook
For
SY-8100 HPLC System

and
software of workstation
SY-8100 HPLC Chromatograph
CONTENT
CHAPTER ONE
PREFACE
SOPs in this manual...............................................................................................1

Unpacking ...............................................................................................................1
Packing list..............................................................................................................1
CHAPTER TWO
HPLC PUMP
Brief descriptions of HPLC Pump ..........................................................................2

1. Front view of HPLC Pump ..............................................................................2
2. Rear panel view of the HPLC pump ................................................................3
Operation of HPLC pump.......................................................................................4
1. Connection of power supply ............................................................................4
2. Users page characteristics and operation........................................................4
Simple Maintenance: ..............................................................................................7
1. Replacing and purging the pump head.............................................................7
HPLC pump technical parameters ........................................................................10
CHAPTER THREE
UV DETECTOR
Brief descriptions of UV Detector ........................................................................ 11

1. General description ........................................................................................ 11
2. Optical path of the UV detector ..................................................................... 11
Preparation of UV detector before the operation:.................................................12
1. Front view of UV detector .............................................................................12
2. Rear view of UV detector ..............................................................................14
3. Power supply, ON/OFF, and self-test.............................................................14
Operation of UV detector......................................................................................15
1. Internal software structure and menu explanation .........................................15
2. Installation of flow cell ..................................................................................17
1
Simple Maintenance..............................................................................................18
1. Lamp maintenance .........................................................................................18
2. Cleaning the flow cell ....................................................................................18
3. Flow cell.........................................................................................................19
Technical data of UV detector ..............................................................................20
CHAPTER FOUR
CONNECTION
Connection of detector to HPLC system ..............................................................21

Connection of UV detector with computer ...........................................................22
CHAPTER FIVE
WORK STATION controlled instrument
Brief instructions of work station .........................................................................23

1. Characteristics of work station.......................................................................23
2. Running environment of work station software.............................................23
3. Integral environment of work station.............................................................24
Operation of work station .....................................................................................25
1. Preparation of work station before the operation...........................................25
2. Operation procedures of work station............................................................27

CHAPTER ONE PREFACE
Thanks for purchasing Model SY-8100 Liquid Chromatograph developed by our company.
This device includes HPLC Pump,UV detector and software of work station. Read this manual
carefully before the operation.
SOPs in this manual
The Standard Operation Procedures (SOP) provided with this manual offer a convenient way of
structuring complex tasks in the operation of your HPLC pump and UV detector. They include
step-by-step instructions leading the user through all routine tasks during the operation. They can
be used for documentation purpose and be copied, applied signed, and filed in order to document
the performance of the instrument.
Please operate the instrument and all accessories according to instructions and SOPs in this
manual. This ensures proper results and longevity of the instrument.
Unpacking
Unpack the instrument and check the device and accessories thoroughly for any damage that may
have occurred during transport. If necessary, put forward any claim for damages to the carrier
and inform of your local dealer immediately.
Use packing list to check the device delivery is complete. Please contact your local dealer if you
miss something or if you need support. Please fill out the guarantee registration card and return it
to the local dealer immediately.
Remove the transport protections from the display.
Packing list
Packing list (Standard delivery)
Quantity
HPLC Pump
1Set
UV Detector
1Set
Standard flow cell
1Pc.
Start-up kit,analytical
1Set
Operation manual
1Copy
Power cable 220V/10A
2Pcs.
RS232 Cable
1Pcs.
Software of work station
1Set

CHAPTER TWO
HPLC PUMP
Brief descriptions of HPLC Pump
1. Front view of HPLC Pump
Fig. 1
Front view of Pump
1-Display information area
2- Foil key area
Display area and key area (1 and 2 in Fig.1)
Fig 2 Display area and key area

1-Display information area
2.1 Pump START/STOP
2.2-Purge START/STOP
2.3-Numeric key pad
2.4-Arrow keys and ENTER key
3-Pump front door

Connections of piping (3 and 4 in Fig, 1)
Fig.3
Piping connection of Pump head with pressure transducer
3.1-Backflushing capillaries
3.3-Head set screws
3.5-Eluent outlet
4.2-Peek screws by hands
4.4-Eluent outlet
3.2-Outlet valve housing

3.4-Inlet valve housing
4.1-Inlets to transducer
4.3-De-areation screw
2. Rear panel view of the HPLC pump
Fig. 4 Rear panel view of the HPLC pump

4.1 Power connector
4.2 Fuse compartment
4.3 ON/OFF Switch
4.4 12V Ventilator
4.5 12V Ventilator
4.7 Gradient program trigger
4.8 Plug for solvent organizer
4.9 RS-232 Interface

Operation of HPLC pump
HPLC pump is controlled by keyboard and work station of the computer control. The following
describes the operation of the keyboard. The work station of the computer control is described in
the Chapter four.
1. Connection of power supply

HPLC Pump is equipped with a universal power supply for input voltage 22020V Ac,
frequency 50 0.5Hz. 1KVA stabilizer is required. Connect the power cable to the socket on
the rear panel of the instrument and switch on the device with the ON/OFF key, see (4.1) and
(4.3) in Fig.4.
CAUTION: Make sure that the main power supply is properly grounded and a
correct three-pronged power cable is used. Connecting the instrument to faulty power
sockets can cause damages.
2. Users page characteristics and operation

Press the key in 2.2 of Fig.2 at any time to make system purge, again press this key to stop
purging.
CAUTION: If the purge is required, first loose PEEK screw by hands, see 4.2 in
Fig3, otherwise chromatographic column will be damaged!
Self-test page
After switching on the instrument, firstly enter into the self-test status, display the self-test page
as shown the follows:
BFRL
SY-8100 HPLC PUMP

RAM TEST OK
BFRL SY-8100 HPLC PUMP

EEPROM TEST OK
Page of switching on
FLOW0.00ml P.max 19.0 MPa
A
PRES 0.0mPa P.min 0.0 MPa STOP
Turn on the switch of the pump on this page. After completing the self-test of the pump, the
above page is appeared. Press 2.1 key in Fig.2 to make the pump START/STOP.
CAUTION: Never run the HPLC Pump without liquid in the pump head or in the piston
backflushing compartment. Operating the pump without solvent may lead to damage of the
pump seals
SOP 1:
4

Press ENTER key, move the triangle cursor among Flow, P. max. and P. min.
As shown in Fig., the triangle cursor points Flow, use the numeric key for flow input, again
press ENTER key to confirm the setting. Therefore, the system flow is transferred to the flow
value of setting.
As the same operation, press ENTER key to move the cursor to P. max. and P. min.
The data value enables to be renewed with the same operation.
Press the arrow andkeys to enter into the other pages.
Vocabulary explanation:
Flow
Current flow of pump
P. max. Maximum pressure allowable for the system
A Pump Series number of Pump
Pressure Current pressure of pump
P. min.
Minimum pressure of system
Stop
Running status of pumpWhen pump stops, displayStop, when
running, displayRun
Gradient mode page
GE MODE: High-Pressure Gradient A
1: HP. GE A 2: HP. GE B
3: LP. GE
This page is the selection page of the gradient mode of the current pump
Press the numerical keys 1, 2 or 3, again press ENTER key to select the corresponding gradient
mode.
SOP 2
Press 1, 2 or 3 keys, again press ENTER key to select the corresponding gradient mode.
Press andkeys to enter into the other pages.
HP. GE A Pump located in A pump system of binary high pressure system
HP. GE B B pump in binary high pressure system
LP. GE
Pump in quarter low pressure gradient system.
During the operation of high pressure gradient and low pressure gradient, the corresponding
setting is required in SY8000 chromatograph work station to realize the work station to fully
control the instrument.See the control section in the operation manual of data work station of
chromatograph.
Pressure zeroing page
Pressure zero Adj

ENTER to zero adj
This page is the pressure zeroing page. There is no need for the user to operate it. User can press
ENTER key to adjust the pressure zeroing unless PEEK screw is loosen and the pressure
value does not display the zero in the page of switching on the instrument.
SOP 3
5

Press ENTER key to adjust the pressure to be the zero.
Pressandkeys to enter into the other pages.
Pressure zero Adj Current pressure display value in the system is adjusted
to be the zero.
Status page
SN: 815299
Total time
Total volume
0.1 (H)
0.1 (L)
This page is a using status page of current pump, the user can not operate it any more.
SOP 4
Pressandkeys to enter into the other pages.
SN Production series of the instrument, not series No. of the delivery
Total time
Accumulated using time of current pump.
Total volume Accumulated transfusion volume of current pump.
Remark page
BFRL HPLC PUMP

SN. 815299
Rev: 1.7
This page shows Model of HPLC Pump, Series No and Version No. It is not necessary for
the user to operate it.
SOP 5
PressandKeys to enter into the other pages.
BFRL HPLC Pump
SN. 815299
Rev: 1.7
Model of instrument
Production number of instrument
Software version No.

Simple Maintenance:
1. Replacing and purging the pump head

1.1 Disassembling the pump head
CAUTION: If the organic residential is remained in the pump head, it may cause the
skin irritation..
Use proper cleaning reagent to purge the pump head, and then to purge it with the distilled water.
After so, `the replacement for the pump head is made.
SOP 6:
1. LoosenEluent outlet, see Fig.33.5
Loosen inlet valve housing, see Fig. 3(3.4).
2. Use 3#3mmhexagonal spanner to loose fixing screws of pump head on the two
slanting angles, see Fig. 3 (3.3). Remove them.
3. Carefully loosen the rest two screws about half a turn, alternating from one to the other
to avoid the damaging the pump head. Unscrew the two screws strictly alternating due
to strong force of springs, it may be help to either clamp the pump head or to press one
of its side surfaces against a table with one hand and remove it.
4. Carefully remove the pump head
Disassembling and Check piston
SOP 7
1. Detach the pump head.
2. The piston is shown as Fig.511. A clipper (nipper) is available for detaching it.
Use the clipper to clamp the metal material part of the piston end and carefully pull it out along
on the straight line.
CAUTION:
If the piston is broken, the pump head must be thoroughly checked for the damage.
1.2 Detaching the pump head

1) Detach the pump head.
2) Detach the piston.
3) Loosen the two retaining plate screws as shown in Fig.520half a turn,
alternating from one to the other to avoid damaging the retaining plate.
4) Because the two screws are very tight, it may be help to either clamp the pump head or
to press one of its side surfaces against a table with one hand while loosening the
screws.
5) Unscrew the screws strictly alternating due to strong force of the spring behind the plate
as shown in Fig. 510and remove them.
6) Dismount the retaining plate, see Fig.512.
7) The moment, remove spring guide sleevesee Fig.519and spring:.
8) Use the spinner to loosen retaining nut as shown in Fig.59, half a turn alternately.
9) Remove Rear pump head as shown in Fig 517and take out15 and16.
10)Insert the piston into high pressure seal ring, and then pull it out. The moment, the
seal ring is adhered on the piston and take it out.
Disassemble diagram of the pump head and parts sequence.

Ser. No
Parts Name
Retaining screw of pump head
Front pump head
Front connection tube
High pressure seal ring
Stainless steel block ring
O- ring
Rear purge tube
Low pressure seal ring
Retaining bolt
10
Spring
11
Piston
12
Retaining plate
13
Valve sleeve
14
Single way valve
15
Plastic baffle ring
16
Rear purge tube
17
Rear pump head
18
Bushing
19
Guide for spring
20
Screw of retaining plate

Table 1
Pump head

1.3 Assembling the pump head
All positions of components of the pump head refer to Fig 5
SOP 8
1. Detach the pump head to check it. After solving the problems, mount it into (4
15
and5at the front pump head in regular sequence.

2. After mounting the pump head, piston the head in a straight line onto the pump
housing (9and then take the piston out .
3. Mount spring guide sleeve and spring, see Fig.519and (10).
4. Install retaining plate, see Fig.512.
5. Insert and tighten the two screws strictly alternating due to strong force of the spring
behind the plate.
6. Alternating from one to the next, tighten two screws half a turn at a time on the
retaining board so as not to damage it.
7. Carefully insert the piston rod, see Fig.511without bending or quenching the rod..
8. Both the spacing bolts and retaining board screws must be tightened securely
as before.
1.4 Installing the pump head
SOP 9:
1. Make sure that the pump head has been correctly assembled.
2. Piston the head in a straight line onto the pump housing..
3. Tighten all four pump head set screws a few turns by hand, see Fig.510.
4. Alternating from one to the next, tighten two diagonally opposed screws half a turn at a
Time until the pump head is correctly seated
5. Tighten the two remaining screws. Make sure that all four pump head set screws are
Securely tightened, see Fig. 5 (1).
6. Mount the capillary connection between the pump head outlet and the pressure
transducer.

HPLC pump technical parameters
Flow rate range:
Structure of Pump:
0.019.99 ml/min
Dual piston rod, series type oscillating
displacement pump
Flow accuracy
<1%
Flow reproducibility 0.5%
Pressure pulsation
<0.04MPa
Pressure range
042MPa
Remote control
RS-232 interfaceComputer control
System protection
Available for protection of minimum and
maximum pressures
Display
LCD 216
Dimension
350190210 mmLWX H
10

CHAPTER THREE UV Detector
Brief descriptions of UV Detector
1. General description
BFRL UV detector is a valuable new type detectorespecially developed for the routine analysis.
The deuterium lamp is used as the light source of the instrument.
UV detector selects the flow cell of standard typeOptical path:10mmvolume:10Lthe flow
rate range of eluent phase of UV detector is available from 0.0019.999mL/min.
After powering up, the UV detector performs the self test and calibration automatically.
z The internal software includes an auto-calibration of the wavelength scale. The
position of the grating reflection (0. order) will be located automatically.
z The deuterium lamp with the wavelength of 656nm is used to check and to calibrate
the display.
The distance of the wavelength passed through the monochromator should be 1nm for per
step, arbitrary setting can be made in the range of 190nm to 740nm. Accuracy is 2nm.
In addition, UV detector still have the function of the whole wavelength or the wavelength
range set by user to process stopping the flow scanning.
UV detector provides with very low noise210-5AUand shift1510-5AU/havailable
for auto-zeroing of the full scale.
Key pad is clear, clean film, convenient for completing the operation.
The control and data acquisition of the UV detector is digitalization to secure running
without the trouble.
2. Optical path of the UV detector

1. Deuterium lamp
2. Slit
3. Filter
4. Concave mirror
5. Plane grating monochromator
6. Half transparent mirror
7. Flow cell
8. Photodiode (Sample)
9. Photodiode (Reference)
9.
Optical Path of UV detector
Instructions for optical path

A beam of emitted light from the deuterium lamp1is faded out by a slit2. After passing a
filter3 it is focused by a concave mirror4on a plane grating monochromator 5so
as to obtain a beam light of characteristic wavelength. The bream is then split by a half
transparent mirror6. One part beam delivers the reference signal9and the other after
pathing the flow cell7irradiates to photodiode (8) to produce the measurement signal.
11

Preparation of UV detector before the operation:
1. Front view of UV detector
1.Display information area

Fig.7
2.Foil key pad
3.UV detcector front door
Front panel of UV detector and flow cell
Display area and foil key pad
Fig. 8
Display area and foil key pad
1. Display area
2. Control function key
3.Input key
4. Arrow key and ENTER key
Information display area

ABS0.0001 AU
LAMP IS LIT
WAVE
254 nm
Fig. 9
Display information area
Information display area displays real time parameters and result data of UV detector.
Foil key pad area
Foil key pad includes control function keyAuto-zeroing key and running program key,Data
12

input key, arrows keys and ENTER key.
Auto
Zero
Prog
Auto-zeroing
Press this key to adjust the baseline of the instrumentbaseline returns to zero.
Normally press this key before the analysis. Use sampling valve of signal cable
(7725i) equipped with the instrument for sampling, the work station will
automatically control to process the zeroing adjustment for once at the same time
the valve is changed over. The moment, there is no need for user to press this key
again.
Running program
Press this key to run the wavelength scanning program set by user.
Data input key
As shown in Fig.83. Data input key is used to modify the operation parameters.
At the same time, CE key is set at the key areaCANCEL key. It is used to
cancel error parameters being input.
Arrow keys and ENTER key

As shown in Fig.84. Arrow keys are used to turn over the pages. Enter key is
used to confirm currently entered or selected parameters.
Flow cell area
Fig. 10
Flow cell area and flow cell
1. Knurled cell screw
2. Flow cell cradle
3. Inlet liquid
4. Cell body
5. Outlet liquid
Inlet liquid of the flow cell is connected to column, outlet liquid is connected to waste liquid
bottle or collecting device. Every constituent separated by the column is entered into the flow
cell to make the determination.
13
2. Rear view of UV detector
Fig. 11
Rear view of
UV detector
1.RS232 interface
2.Remote terminal
3. Analog output
4. 24V ventilator
5.12V ventilator
6. Power switch
7., power socket
8. Slow melting fuse1A
3. Power supply, ON/OFF, and self-test

UV detector operates with 22020V AC of 500.5Hz. 1A slow melting fuse is used. The
setting is done by the manufacture on custom request. The actual setting is indicated on the
rear panel of the instrument (Fig. 12).
Caution:
Cut off power supply before opening cover.
22020V 500.5 Hz
100VA
FUSE: 1A SLOW
Fig. 12 Indication of the input voltage
CAUTION: Make certain that the correct voltage has been set on the rear panel of the
instrument, the power supply is reliably grounded and a corresponding 3-pole power cable
is used.
Connect the detector to the power supply. Switch on the instrument. Power switch is on the rear
part of the instrument.
After switching on, the instrument first performs the self-test as follows:
BFRL
RAM
UV/VIS
TEST
DETECTOR
OK
14
BFRL UV/VIS
EEPROM TEST
DETECTOR
OK
>
Afterwards, display model of instrument and simultaneously the deuterium lamp is lit.
BFRL UV/VIS
Pre-heating
DETECTOR
Power on the instrument a time, the wavelength should be calibrated for once, display as
follows:
BFRL UV/VIS
Check Home Pos
DETECTOR
After a certain period of zeroing calibrationabout one minute, display as follows

BFRL UV/VIS
Wave calib
DETECTOR
The detector enters into the following page afterward. This page is also a acquiesce page of
UV detector
ABS
WAVE
0.0001 AU
254 nm
LAMP IS LIT
15 minutes later, UV detector is ready for the analytical determination.

Operation of UV detector
1. Internal software structure and menu explanation

The software is divided into several menus. Settings and operation modes are made on every
menu. Useand keys to enter into each menu as shown in Fig.13.
ABS
WAVE
0.0001 AU
254 nm
RESPONSE
LAMP IS LIT
1.00
0.0510S
Press [ENTER] TO Change
15
D2 LAMP ENERGY
USED TIME 50.3 h
SMPL 0.794583
REF.
0.401205
SERIAL
825365
1:D2 OFF 2WAVE CALIB
Option
[Enter]
BFRL UV/VIS DETECTOR

SN. 825282
Rev: 1.70
Fig. 13
Menu sequence of UV detector
Describe every menu in details as follows. When the parameter on the menu is required to
modify, use the data input key to modify the setting of the corresponding parameter. Thus press
the ENTER keythe moment the value just input is confirmed. If error parameter is input, use
CEkey to delete it, then input the correct one according to the procedures mentioned above.
Signal menu
ABS
-0.0001AU
WAVE
254nm
LAMP IS LIT
Signal menuor main menuincludes: absorbancesignal intensityD2 lamp status and

wavelength. Absorbance is in unit of AU. D2 lamp status shows ON and OFF. The
wavelength is in unit ofnm.
The signal value for real measurement displays in four digits. The wavelength range is from 190
to 740nm for arbitrary setting.
Response time menu
RESPONCE
1.00 0.0510S
Press [Enter] TO Change
Use the response time (time constant) to make the signal smooth. Press ENTER key to select the
input. Respectively set: 0.050.100.150.200.250.300.350.400.450.500.55
0.600.650.700.750.800.850.900.951.001.502.002.503.003.504.00
4.505.006.007.008.009.0010.00sec. The value is more bigger, the signal is more smooth.
Time constant of 1second. is satisfied with the most of the analysis requirements.
D2 lamp energy and using time menu
D2 LAMP ENERGY
USED TIME 50.3 h
SMPL 0.794583
REF 0.401205
D2 lamp energy menu displayed is the energy values of ultraviolet light received from the
16

photodiode on the reference signal board and the sample of the detector. These two energy
values can be directly known the energy of the deuterium lamp. If these energy values show too
small, replace D2 lamp on time.
The used time means the ignition time of D2 lamp.
Series No. and operation function menu
SERIAL 825365
1D2 OFF 2WAVE CALB
Option
[Enter]
Series No. means production series No. of the detector, not delivery series No.
Operation function menu includes:1OFF lamp
, 2Wavelength calibration. Respectively
input the numeric number corresponded to the operation function under this menu. Press
Enterkey to complete the corresponding function. For example: Complete the operation of
switching off the lamp, press the corresponding digit1under this menu and then pressEnter
key to finish the operation of OFF lamp. As the same, complete the operation of the wavelength
calibration, press the corresponding digit2on this menuafter so, pressEnterkey to finish
the operation of the wavelength calibration.
Identification menu
BFRL UV/VIS DETECTOR
SN. 825282
Rev: 170
Identification menu displays model and name of the instrument simultaneously display
production series number of the instrument and software version No. It is not necessary for the
user to modify any item in the identification menu.
2. Installation of flow cell

The flow cell of UV detector has not been delivered during transport. There is a dummy flow cell
without any of optics equipped with the instrument. Before the detector is used, a proper
standard flow cell must be installed.
CAUTION: There is no need to change the status of the instrument and the lamp
during the replacement of the flow cell.
SOP 1 Installation of flow cell
This instruction refers to UV detector as shown in Fig10 flow cell area and flow cell (see the
preparation of UV detector before the operation in the concerned section).
a) Loosen the two knurled cell screws (1) by hand and remove them.
b) Pull out the flow cell cradle (2).
c) Take the cell or dummy cell with two figures and remove it upward.
d) Insert the new flow cell and make sure engraved specification towards the user
(.can be read) and the fixing hole on the back side of the cell meets the
corresponding metal pin.
e) Now push the complete system towards the housing, insert the two screws and
tighten them manually.
17

Simple Maintenance
1. Lamp maintenance
The deuterium used with UV detector has an extended lifetime to ensure long-time functionality
and reliable measurements with low noise and baseline drift as well as high sensitivity. (life time
about 2000 hours). The actual using time of the deuterium lamp depends on different factors, like
the number of lamp starts, the average burning time and your requirements concerning noise and
sensitivity.
To check the functionality of the lamp, the two intensity values of sample and referenceseeD2
lamp energy and used time menuthe help information for D2 lamp can be found there. The
reference value refers to the intensity of the light measured in the reference channel and be used
for checking the quality of the lamp.
We recommend to check the reference value at regular intervals under the conditions mentioned
abovedummy cell, = 240nm. This applies especially in case higher noise levels or decreased
sensitivity is observed on working with UV detector. In these observations coincide with a
reference value of approx. 0.1 or less, or used time is over 2000h, a new deuterium lamp should
be installed.
2. Cleaning the flow cell

Noisy baselines and low sensitivities may be due to a dirty flow cell. This may also be indicated
by a low absorbance value in the signal menu when flushing the cell with pure solvent. That
shows the flow cell has been polluted. In most cases it is sufficient to purge the flow cell
according to the following SOP.
SOP 1 Purging the flow cell
This instruction applies to the flow cell delivered with UV detector
a) Purge the flow cell using one of following solvents: sodium dodecyle sulfate
(SDS), 1m HCL, 1m NaOH, ethanol, or acetone.
b) Run the solvent through the flow cell using a syring and leave for approximately 5
minutes.
c) Rinse extensively with water and the blow dry using a gentle stream of pure
nitrogen.
CAUTION: Never dry with compressed air to blow the flow cell, this will contain
microdroplets of oil that will coat the cell.
CAUTION: When the optics module is not in use, disconnect the flow cell and clean
outer traces of salt and protein with a syringe filled with distilled water.
Before storing the flow cell inject a dilute solution (10-25%) of ethanol or
i-propanol to prevent microbial growth.
CAUTION: In case the flow cell purging do not provide sufficient success, all flow cells
can easily be disassembled for cleaning the lenses.
18
3. Flow cell
Fig.17
Sectional view of
flow cell
SOP 2 Cleaning the flow cell

This instruction applies to the flow cells enclosed in UV detector.
a) Unscrew the outer threads with 3mm hexagonal spanner enclosed in the flow
cells delivery.
b) Remove the black gasket that carries the lenses with a pair of tweezers or by
gently tapping it on a clean surface. The lens is embedded in the gasket and sealed
against the flow path with PTFE seal. This seal should be changed every time
when assembling the flow cell.
c) Take out the lenses and clean them by wiping them with a soft cloth or with an
appropriate solvent in an ultrasonic bath. Be careful not to touch the lenses
with the figures.
d) Reassemble the cell in the reverse manner, making sure that PTFE seal does not
block the light path.
e) Tighten the outer threads carefully with the spanner in order not to damage the
lenses.
19

Technical data of UV detector
Wavelength
Lamp
Wavelength accuracy
Scan function
Measurement range
Sensitivity
Noise
Baseline drift
Time constant
Integrator output
Auto-zero range
Display
Control
Power supply
Weight
Dimensions
190~740nm
Deuterium lamp
2nm
Spectral scanning
0~2AU
410-5AU at 240nm and time constant 1.0S
210-5AUat 240nm and time constant 1.0S
1510-5AUat 240nm and time constant 1.0S
0.0510.00
1.0V
Full scale
216Digits
RS232interface, analog output, remote connector
22020 V500.5Hz100VA
8Kg
350190210mm (LWH
20

CHAPTER FOUR
Connection
Connection of detector to HPLC system

CAUTIONBefore taking a measurement cell filled with fluid into operation, please
make certain that the reagent used is miscible with that one used
previously. Or purge the flow cell with a medium miscible and two
miscible reagents.
The connection of the fluid piping in the isocratic system is shown in the following Fig.
Convenient for the instruction, the pump head represents HPLC pump and UV detector shows as
flow cell.
15
Isocratic HPLC system
Brief description of fluid system

a) Flow path of fluidAs shown above the Fig, Fluid is aspirated from sampling inlet
by HPLC pump, then flow into the sampling valve (2) from the fluid outlet,
afterwards, from the sampling valve (3) to the column. The fluid is entered into
the flow cell of the detector coming from the column. At last enter into the waste
liquid bottle or collection device.
b) Flow path of sampleAs shown in Fig 16, see the operation mode of the 7725i
sampling valveThe operation of 7725i sampling valve is divided into two parts:
preparation status and sampling status.
Preparation status
Fig. 16
Sampling status
Operation mode of 7725i sampling valve
Preparation status: The pump is directly connected with the columnconnection

of hole 2 with hole 3and the needle hole is connected with the sample ring. The
moment, use the sampling needle to aspirate 2-3 times sample (or more, to
21

achieve the higher accuracy) of sample ring volume to inject it into the sample
ring. The residual sample is drained off through the discharge tube which is
connected with the hole 6.
Sampling status: The pump is connected with the sample ring (hole 1 and hole
2)Purge all of samples from the sample ring to the column. Needle port is
connected with the discharge tube (hole 5).
The sample under the sampling status is brought into the column by the fluid for
the separation.
Connection of UV detector with computer
UV detector is connected with computer by RS232 interface. The analytical result enables to
display in the software of HPLC chromatograph by this interface. The software of HPLC
chromatograph can be also realizing the modification of the detection parameters by this
interface..
RS-232 series interface is located on the back of the instrument as shown in Fig.11see the
section described in the preparation of UV detector before the operation. Use this interface to
connect the computer installed the software of HPLC chromatograph with UV detector.
The series interface of the computer is directly connected with this interface.
22

CHAPTER FIVE
Work station controlled instrument
Brief instructions of work station

The work station controlled SY-8000 series HPLC Chromatograph is a specialized instrument
controlled software. To realize full control of the instrument, user can input control parameters of
the instrument in the work station for controlling running of the instrument so as to avoid the
operation on the instrument panel manually.
The section only describes how the work station to control the instrument, the data processing of
the work station refers to operation manual of SY-8000 Chromatograph work station.
1. Characteristics of work station

All in one data file structure: including method file, data file, report module file and sample
queuing file.
Method file---- including peak integral parameter, quantitative parameter, constituent table,
calibration curve, report format, method information and instrument conditions, etc. File
expended namemth, file path as work station directory\mth.
Data file--------- including chromatogram data, sample information, peak table, analytical result
and method. File extended name DAT, file path at will.
Report module file---Save the required parameters for printing the specialized report. File
extended name flm, file path as work station directory\REP.
Sample queuing file----Save sample name, multiplier and divisor, etc. File extended nameSDL,
file path at will. It is available for analyzing the batch sample continuously.
There are another three files including in the data file. Those three files are respectively
independent file. It is very convenient for user to recall the required file freely. The structure of
all in one data file can be able to open the file from the method file, or from the sample data file.
Custom report tool: The custom report editor provides the user for self designing the report
content and the required format. It is similar to the character treatment program. Therefore, the
characters, color and margin for page can be changed and available to drive block (the block is
copied to the cut and paste board, and then the block is adhered to word or wps file). Insert the
chromatogram, graphics, system information and format, etc to let user flexible edit the required
report, adjust its appearance, contents and location.
The report module is used to control the printing format file in the chromatograph work station.
All of parameters required for printing the report are saved in the report module file and these
parameters will directly influence on the report appearance. The report module can be designed
by yourself with custom report (custom report editor). The report designed can also be saved
as a new module. The system is automatically acquiesced and the module file extended name
is flm , Saving path: installation directory of work station\REP.
Multi-chromatogram result report: The custom report editor still provides the analytical function
of multi-overlapped chromatogram. That means, If multi-parallel experiments are required for
testing the reproducibility and the average value, the report system will directly calculate result
mean value.
Prompt operation page: Pages are very clear, easy operation. Such as: editing page of all
parameters in the method file is saved in the window of the method file edition in form of cards.
2. Running environment of work station software

Hardware: Better 586, CPU
23

Better 64M
One free series interface on the system board (COM1 and COM2)
VGA Color display (better use an accelerated card of graphics)
Optical drive
Mouth
Printer
Software: Better Windows 20000 operation system
3. Integral environment of work station

Double-click the graphic of chromatograph work station on the table to enter into the intergral
environmental page of work station.
Main menu
Prompt button
Running indicating lamp
Spectrogram display window
Information line
Fig 1
The main page of the integral environment consists of five parts: main menu, graphic display
window, prompt button, controlling menu, running indicating lamp and information line.
Main menu
As same as the other application program, according to the different requirement of treating data,
all of the function commands are classified, after so, respectively put them into eight menus, so
every menu has own group commands.
Prompt button
Some common used functions in the main menu are marked on these buttons with display
presentation. Therefore, these functions in common use can be directly selected by pressing the
concerned button without selecting it from the menu in order to speed up the operation.
Spectrogram display window: including plotting area, time coordinate scale, level coordinate
scale and peak hold time.
Plotting areaActively display the real time spectrogram area, the sizes can be automatically
adjusted with the size change of the spectrogram display window. When the user makes the
spectrogram display window shortening to a certain extent, the system automatically delete the
24

plotting coordinate scale and display of the hold time to secure observing the graphics clearly
even in a small size window.
Time coordinate scaleIt is used as X axle coordinate scale, in unit: Min. The initial value is
automatically adjusted with the change of the sampling time. Modify the full time difference on
screen plotting parameter edition, minimum time is 1min.
Level coordinate scaleIt is used as Y axle coordinate scale of the spectrogram plotting, in
unit : Mv. Modify the full scale maximum value and minimum value on screen plotting
parameter.
Peak hold time During starting sampling, automatically display the hold time after
appearing the chromatograph peak top. In the screen plotting parameter edition, select no
display the hold time to close it.
Running indicating lamp
The red lamp is flash when the data is acquiring, while the green lamp is lit under the state of
preparation
Information line
It displays currently inputting level, sampling time, current sample name and current time.
As the main page mentioned above, when you select some command in the main menu or
single-click some of prompt button, the corresponding window or dialogue block will be
appeared.
Operation of work station
1. Preparation of work station before the operation

Before operating the work station, SY-8000 series HPLC Chromatograph should be connected
with the computer installed the work station software. Power the instrument on. When
completing the self test of the instrument, the computer displays the following page, That shows
the connection of the computer is successful.
Current Wavelength
Success for the connection
Current
setting
flow
Fig 2
25
Current
pressure
of

After success for the connection of the instrument with the computer, the operation page of the
work station simultaneously display current setting flow speed and current pressure of pump and
current wavelength of UV detector.
If the instrument failed to connect to the instrument, under instrument control menu, make
setting of communicating terminal and let the terminal number of the setting be in accord with
the terminal number connected with the instrument shown as the following figure:
Setting of communicating terminal

Fig. 3
After success for the connection of the instrument with work station, firstly click Pump or
Detector on the work station, check the setting of working parameters of the pump and
detector as shown in the following:
Setting of pump
Fig. 4
26
Setting of detector
Fig. 5
The pump requires to set the control mode (control mode divides: single pump, double pump
running independently, double pump high pressure gradient and single pump low pressure
gradient), If using gradient table, pump flow and up limit and down limit of pressure; Detector
requires to set the wavelength and deuterium lamp status, if using the wavelength program table,
etc. The sensitivity of the detector is set by the acqueuiescence. The spectral scanning is set
according to the concerning conditions when it is required.
2. Operation procedures of work station

Use the work station software to do the experiment after the settings mentioned above. As
example for outer standard method as follows:
Operation procedures of outer standard method
1. Create a new method file parameter to do the experiment. Its procedures are as follows:
1) Start HPLC chromatograph and work station software;
Fig. 6
27

2) Record parameters of editing data
Signal-click the graphic of data record parameter to appear the window of the method editing,
set the position of file storage.
Fig.7
3) Editing integral parameter
Single-click the graphic of integral parameter to appear the window of the method edition.
Where set the integral parameter value and edit time program table, If it is not set any, only
press the acquiescent value.
Fig. 8
4) Edit sample information table
Click the graphic of sample information, edit sample information table, define the name of
the experimentalized data file as sample name as shown in the following:
28
Fig.9
5) Standard sample anslysis
When running the chromatograph stable, input the standard sample. During valve sampling, at
the same time, the program automatically process the data acquisition to the spectragram, and
remark the hold time. When all of peaks are appeared, stop the program manually, or set stop
time in integral parameter table to stop it automatically.
6) Sample analysis
Input the unknown sample. As same as the procedures 5. (this step can be made at last.) Data file
obtained from the procedures 5 and 6 will be saved automatically by the system.. The data files
are automatically, continuously named. Such as: Test001.dat, Test002.dat, Test003.dat..
7) Draft the calibration curve
a. Edit the quantitative parameter
Click the graphic of the quantitative parameter to appear the window of the method
edition:
Fig. 10
Set well quantitative mode, quantitative method (outer standard method), standard
( eight concentration standard sample, select eight points), standard concentration
unit and sample result unit.
b. Filling in constituent table
Click the graphic of constituent table to appear the window of the method edition, fill in
the analytical sample constituent table name, hold time and series standard concentration.
29
Fig.11
c. Definition calibration curve
a) Click the graphic of calibration curve to appear the window of the method edition.
Fig.12
b) Definite spectrogram peak file required at every point. Firstly use the mouth to move
to the point number of the standard sample, then click the right key, this number is
covered by blue frame, again click the left key to select the menu:
Fig.13
c) Select Add item (this operation shows at the same concentration point, add the data
30

of several parallel experiment to calculate the mean value), the window of
spectrogram selection is appeared.
Fig.14
Here select the corresponding spectrogram file. + appears before the file of sequence
number point. After completing the definition foe every points, click re-read peak file to
display the calibration curve.
8) Check sample result
Enter re-treatment page, click the graphic of open data file to appear the window of
spectrogram selection, select the sample spectrogram file here as follows:
Fig.15
And then click the button of re-calculate, Again click the graphic of screen report to look at
the screen report table.
31
Fig.16
9) Printing report
2. Recall the method file parameters originally saved to do the experiment.
1) Use the mode of open method
Select open method in file menu of work station, appear the window of method file
selection, select the method file here as shown as follows:
Fig.17
Sampling is directly made after recalling the method file without the operation procedures from
1-7. After the experiment, the step 8 is made directly to look at the sample content.
2) Use mode of open method from data
Select open method from data in file menu of work station (it is another mode for recalling
the method file, only recall the method file with the date), appear the window of spectrogram
selection, the moment, select the method file here as shown as the follows:
32
Fig.18
The descriptions as same as the method above mentioned, Sampling analysis can be made after
recalling the method file without the operation procedures from 1-7 steps. The procedure of step
8 is made after completing the experiment in order to directly obverse the sample content.
33

SY-8100 Manual Operacion

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Users Handbook

SY-8100 HPLC System

SY-8100 HPLC Chromatograph

SOPs in this manual...............................................................................................1

Brief descriptions of HPLC Pump ..........................................................................2

Brief descriptions of UV Detector ........................................................................ 11

SY-8100 HPLC Chromatograph

Connection of detector to HPLC system ..............................................................21

WORK STATION controlled instrument

Brief instructions of work station .........................................................................23

SY-8100 HPLC Chromatograph

Standard flow cell

Power cable 220V/10A

Software of work station

SY-8100 HPLC Chromatograph

Brief descriptions of HPLC Pump

1. Front view of HPLC Pump

Front view of Pump

1-Display information area

2- Foil key area

Display area and key area (1 and 2 in Fig.1)

Fig 2 Display area and key area

2.1 Pump START/STOP

2.3-Numeric key pad

2.4-Arrow keys and ENTER key

3-Pump front door

SY-8100 HPLC Chromatograph

Piping connection of Pump head with pressure transducer

3.2-Outlet valve housing

2. Rear panel view of the HPLC pump

Fig. 4 Rear panel view of the HPLC pump

SY-8100 HPLC Chromatograph

1. Connection of power supply

2. Users page characteristics and operation

SY-8100 HPLC PUMP

BFRL SY-8100 HPLC PUMP

SY-8100 HPLC Chromatograph

Pressure zero Adj

SY-8100 HPLC Chromatograph

BFRL HPLC PUMP

SY-8100 HPLC Chromatograph

1. Replacing and purging the pump head

1.2 Detaching the pump head

SY-8100 HPLC Chromatograph

Disassemble diagram of the pump head and parts sequence.

Retaining screw of pump head

Front pump head

Front connection tube

High pressure seal ring

Stainless steel block ring

Rear purge tube

Low pressure seal ring

Single way valve

Plastic baffle ring

Rear purge tube

Rear pump head

Guide for spring

Screw of retaining plate

SY-8100 HPLC Chromatograph

and5at the front pump head in regular sequence.

SY-8100 HPLC Chromatograph

SY-8100 HPLC Chromatograph

2. Optical path of the UV detector